{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:37:30Z","timestamp":1790620650866,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T00:00:00Z","timestamp":1755907200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003720","name":"Korea Atomic Energy Research Institute","doi-asserted-by":"publisher","award":["523320-24"],"award-info":[{"award-number":["523320-24"]}],"id":[{"id":"10.13039\/501100003720","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003720","name":"Korea Atomic Energy Research Institute","doi-asserted-by":"publisher","award":["2022R1A2C2013345"],"award-info":[{"award-number":["2022R1A2C2013345"]}],"id":[{"id":"10.13039\/501100003720","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["523320-24"],"award-info":[{"award-number":["523320-24"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["2022R1A2C2013345"],"award-info":[{"award-number":["2022R1A2C2013345"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Isoegomaketone [(E)-1-(furan-3-yl)-4-methylpent-2-en-1-one; 1] is abundant in the essential oil of Perilla species and exhibits various biological activities, such as anticancer and anti-inflammatory effects. In order to discover compounds with reduced toxicity or enhanced biological activity through structural modification of natural product-derived components, isoegomaketone was irradiated with an electron beam at five different doses, and (\u00b1)-8-methoxy-perilla ketone (2) was obtained with the highest yield of 3.8% (w\/w) at 80 kGy. Its structure was identified by one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy and high-resolution chemical ionization mass spectrometry. Compound 2 inhibited nitric oxide production and inducible nitric oxide synthase mRNA expression in a dose-dependent manner in lipopolysaccharide-stimulated RAW 264.7 cells. It also dose-dependently suppressed the mRNA expression of pro-inflammatory mediators such as IL-1\u03b2, IFN-\u03b2, and MCP-1, while having no significant effect on IL-6 mRNA levels. Furthermore, ELISA analysis demonstrated that 2 reduced MCP-1 protein expression but did not affect the protein level of TNF-\u03b1 or IL-6. This study provides a reference for the structural analysis of compounds related to 2 by presenting NMR data acquired with chloroform-d, and is the first to report the anti-inflammatory properties of 2.<\/jats:p>","DOI":"10.3390\/molecules30173466","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:09:53Z","timestamp":1756080593000},"page":"3466","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Ionizing Radiation-Induced Structural Modification of Isoegomaketone and Its Anti-Inflammatory Activity"],"prefix":"10.3390","volume":"30","author":[{"given":"Euna","family":"Choi","sequence":"first","affiliation":[{"name":"Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si 56212, Jeollabuk-do, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6604-8862","authenticated-orcid":false,"given":"Chang Hyun","family":"Jin","sequence":"additional","affiliation":[{"name":"Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si 56212, Jeollabuk-do, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trung","family":"Huy Ngo","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Yeungnam University, Gyeongsan-si 38541, Gyeongsangbuk-do, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jisu","family":"Park","sequence":"additional","affiliation":[{"name":"Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si 56212, Jeollabuk-do, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5502-0736","authenticated-orcid":false,"given":"Joo-Won","family":"Nam","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Yeungnam University, Gyeongsan-si 38541, Gyeongsangbuk-do, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7824-4441","authenticated-orcid":false,"given":"Ah-Reum","family":"Han","sequence":"additional","affiliation":[{"name":"Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si 56212, Jeollabuk-do, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8809792","DOI":"10.1155\/2022\/8809792","article-title":"Advances in the Pharmacological Activities and Effects of Perilla Ketone and Isoegomaketone","volume":"2022","author":"Wang","year":"2022","journal-title":"Evid. 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