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In this study, we examine the stability and decomposition rates of adenosine phosphates\u20145\u2032-AMP, 5\u2032-ADP, and 5\u2032-ATP (mentioned simply as AMP, ADP and ATP hereafter)\u2014at temperatures of 22\u201325 \u00b0C, 50\u201355 \u00b0C, 70\u201375 \u00b0C, and 85\u201390 \u00b0C, at a pH of 4, over periods of 2 and 4 days, in both saltwater and ultrapure water, under unsealed and completely dried down conditions. We found that adenosine phosphates degrade rapidly under heat and dehydration, particularly at temperatures above 25 \u00b0C. Among the three compounds, AMP is the most stable, maintaining its integrity between 22 and 55 \u00b0C, whereas ATP begins to degrade at 22\u201325 \u00b0C and ADP is completely decomposed at temperatures above this range. Decomposition rates were analyzed using quantitative 31P-NMR, based on the detection of various phosphorus-containing species. AMP primarily hydrolyzed into phosphate, pyrophosphate and even formed 2\u2032,3\u2032-cAMP. In contrast, the condensed adenosine phosphates (ADP and ATP) hydrolyzed to AMP, phosphate, pyrophosphate, triphosphate, 5\u2032-AMP and, in some cases, 2\u2032,3\u2032-cyclic adenosine monophosphate (2\u2032,3\u2032-cAMP). We also investigated the formation of these compounds in the presence of N-containing additives such as thiourea, urea, imidazole, and cyanamide at temperatures between 65 and 70 \u00b0C. Among these, cyanamide and urea were particularly effective in promoting the synthesis of adenosine monophosphates (AMPs) from phosphate and adenosine. The major products observed were 2\u2032,3\u2032,5\u2032-AMPs and cyclic 2\u2032,3\u2032-AMPs. In some experiments, adenosine diphosphate (ADP) and dimeric nucleotide species were also detected. These findings suggest that moderately heated evaporating pools could facilitate the abiotic formation of AMPs. However, such environments would likely have been unsuitable for the long-term accumulation of these compounds due to continued degradation, though there would exist some level of these nucleotides at steady state.<\/jats:p>","DOI":"10.3390\/molecules30173587","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T12:04:28Z","timestamp":1756814668000},"page":"3587","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Thermal Decomposition and Prebiotic Formation of Adenosine Phosphates in Simulated Early-Earth Evaporative Settings"],"prefix":"10.3390","volume":"30","author":[{"given":"Maheen","family":"Gull","sequence":"first","affiliation":[{"name":"Department of Earth and Environmental Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA"},{"name":"Rensselaer Astrobiology Research and Education Center (RARE), Rensselaer Polytechnic Institute, Troy, NY 12180, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Mehta","sequence":"additional","affiliation":[{"name":"Howard University Plasma Lab (HUPL), Howard University, Washington, DC 20059, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Jesus Herrero","family":"Perez","sequence":"additional","affiliation":[{"name":"Department of Earth and Environmental Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA"},{"name":"Rensselaer Astrobiology Research and Education Center (RARE), Rensselaer Polytechnic Institute, Troy, NY 12180, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika","family":"Seeley","sequence":"additional","affiliation":[{"name":"Department of Earth and Environmental Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA"},{"name":"Rensselaer Astrobiology Research and Education Center (RARE), Rensselaer Polytechnic Institute, Troy, NY 12180, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karyn L.","family":"Rogers","sequence":"additional","affiliation":[{"name":"Department of Earth and Environmental Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA"},{"name":"Rensselaer Astrobiology Research and Education Center (RARE), Rensselaer Polytechnic Institute, Troy, NY 12180, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew A.","family":"Pasek","sequence":"additional","affiliation":[{"name":"Department of Earth and Environmental Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA"},{"name":"Rensselaer Astrobiology Research and Education Center (RARE), Rensselaer Polytechnic Institute, Troy, NY 12180, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2296","DOI":"10.1002\/(SICI)1521-3773(19980918)37:17<2296::AID-ANIE2296>3.0.CO;2-W","article-title":"Energy, Life, and ATP (Nobel Lecture)","volume":"37","author":"Boyer","year":"1998","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Fontecilla-Camps, J.C. (2022). The Complex Roles of Adenosine Triphosphate in Bioenergetics. ChemBioChem., 23.","DOI":"10.1002\/cbic.202200064"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Egel, R., Lankenau, D.-H., and Mulkidjanian, A.Y. (2011). On the Origin of Phosphorylated Biomolecules. Origins of Life: The Primal Self-Organization, Springer Berlin Heidelberg.","DOI":"10.1007\/978-3-642-21625-1"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Toppozini, L., Dies, H., Deamer, D.W., and Rheinst\u00e4dter, M.C. (2013). Adenosine monophosphate forms ordered arrays in multilamellar lipid matrices: Insights into assembly of nucleic acid for primitive life. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0062810"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5171","DOI":"10.1038\/s41467-018-07389-2","article-title":"Searching for lost nucleotides of the pre-RNA World with a self-refining model of early Earth","volume":"9","author":"Hud","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Robertson, M.P., and Joyce, G.F. (2012). The origins of the RNA world. Cold Spring Harb. Perspect. Biol., 4.","DOI":"10.1101\/cshperspect.a003608"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/j.cub.2015.06.016","article-title":"The RNA World as a Model System to Study the Origin of Life","volume":"25","author":"Pressman","year":"2015","journal-title":"Curr. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"9457","DOI":"10.1021\/ja201769f","article-title":"Synthesis of carbohydrates in mineral-guided prebiotic cycles","volume":"133","author":"Kim","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/nchem.2202","article-title":"Common origins of RNA, protein and lipid precursors in a cyanosulfidic protometabolism","volume":"7","author":"Patel","year":"2015","journal-title":"Nat. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/10409230490460765","article-title":"Prebiotic chemistry and the origin of the RNA world","volume":"39","author":"Orgel","year":"2004","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1039\/C7OB03017G","article-title":"Glycosylation of a model proto-RNA nucleobase with non-ribose sugars: Implications for the prebiotic synthesis of nucleosides","volume":"16","author":"Fialho","year":"2018","journal-title":"Org. Biomol. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"11328","DOI":"10.1038\/ncomms11328","article-title":"Spontaneous formation and base pairing of plausible prebiotic nucleotides in water","volume":"7","author":"Cafferty","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5640","DOI":"10.1021\/ja410124v","article-title":"Spontaneous prebiotic formation of a \u03b2-ribofuranoside that self-assembles with a complementary heterocycle","volume":"136","author":"Chen","year":"2014","journal-title":"J. Am. Chem. Soc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2118","DOI":"10.1126\/science.284.5423.2118","article-title":"Chemical etiology of nucleic acid structure","volume":"284","author":"Eschenmoser","year":"1999","journal-title":"Science"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4690","DOI":"10.1021\/acs.chemrev.9b00492","article-title":"Thermodynamics of Prebiotic Phosphorylation","volume":"120","author":"Pasek","year":"2020","journal-title":"Chem. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s11084-009-9172-7","article-title":"Charles Darwin and the origin of life","volume":"39","author":"Bada","year":"2009","journal-title":"Orig. Life Evol. Biosph."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Damer, B. (2016). A Field Trip to the Archaean in Search of Darwin\u2019s Warm Little Pond. Life, 6.","DOI":"10.3390\/life6020021"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"13249","DOI":"10.1002\/anie.201606239","article-title":"Darwin\u2019s warm little pond: A one-pot reaction for prebiotic phosphorylation and the mobilization of phosphate from minerals in a urea-based solvent","volume":"55","author":"Burcar","year":"2016","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Gull, M., Omran, A., Feng, T., and Pasek, M.A. (2020). Silicate-, Magnesium Ion-, and Urea-Induced Prebiotic Phosphorylation of Uridine via Pyrophosphate; Revisiting the Hot Drying Water Pool Scenario. Life, 10.","DOI":"10.3390\/life10080122"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1126\/science.171.3970.490","article-title":"Urea-inorganic phosphate mixtures as prebiotic phosphorylating agents","volume":"171","author":"Lohrmann","year":"1971","journal-title":"Science"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1023\/A:1016513114799","article-title":"Phosphorylation of adenosine with trimetaphosphate under simulated prebiotic conditions","volume":"32","author":"Cheng","year":"2002","journal-title":"Origins Life Evol. Biosph."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1038\/218443a0","article-title":"Phosphorylation of adenosine with linear polyphosphate salts in aqueous solution","volume":"218","author":"Schwartz","year":"1968","journal-title":"Nature"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/BF01809496","article-title":"Phosphorylation mechanisms in chemical evolution","volume":"15","author":"Schoffstall","year":"1985","journal-title":"Origins Life Evol. Biosph."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"16729","DOI":"10.1074\/jbc.M611346200","article-title":"Nucleoside phosphorylation by phosphate minerals","volume":"282","author":"Costanzo","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/BF00927141","article-title":"Nucleoside and deoxynucleoside phosphorylation in formamide solutions","volume":"12","author":"Schoffstall","year":"1982","journal-title":"Origins Life Evol. Biosph."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/BF00927935","article-title":"Prebiotic phosphorylation of nucleosides in formamide","volume":"4","author":"Schoffstall","year":"1976","journal-title":"Origins Life Evol. Biosph."},{"key":"ref_27","first-page":"e2200020","article-title":"Selective Phosphorylation of RNA- and DNA-Nucleosides under Prebiotically Plausible Conditions","volume":"4","author":"Bechtel","year":"2022","journal-title":"Chem.Systems.Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5517","DOI":"10.1038\/s41467-021-25555-x","article-title":"A physicochemical orthophosphate cycle via a kinetically stable thermodynamically activated intermediate enables mild prebiotic phosphorylations","volume":"12","author":"Maguire","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1089\/ast.2020.2264","article-title":"Abiotic Synthesis of Nucleoside 5\u2032-Triphosphates with Nickel Borate and Cyclic Trimetaphosphate (CTMP)","volume":"21","author":"Kim","year":"2021","journal-title":"Astrobiology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1089\/ast.2014.1209","article-title":"Abiotic regioselective phosphorylation of adenosine with borate in formamide","volume":"15","author":"Furukawa","year":"2015","journal-title":"Astrobiology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"15816","DOI":"10.1002\/anie.201608001","article-title":"Evaporite Borate-Containing Mineral Ensembles Make Phosphate Available and Regiospecifically Phosphorylate Ribonucleosides: Borate as a Multifaceted Problem Solver in Prebiotic Chemistry","volume":"55","author":"Kim","year":"2016","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s00239-013-9605-9","article-title":"Prebiotic phosphate ester syntheses in a deep eutectic solvent","volume":"78","author":"Gull","year":"2014","journal-title":"J. Mol. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Gull, M., Mojica, M.A., Fern\u00e1ndez, F.M., Gaul, D.A., Orlando, T.M., Liotta, C.L., and Pasek, M.A. (2015). Nucleoside phosphorylation by the mineral schreibersite. Sci. Rep., 5.","DOI":"10.1038\/srep17198"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/s00239-022-10086-w","article-title":"Organophosphorus Compound Formation Through the Oxidation of Reduced Oxidation State Phosphorus Compounds on the Hadean Earth","volume":"91","author":"Gull","year":"2023","journal-title":"J. Mol. Evol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1038\/nchem.2878","article-title":"Phosphorylation, oligomerization and self-assembly in water under potential prebiotic conditions","volume":"10","author":"Gibard","year":"2018","journal-title":"Nat. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"23781","DOI":"10.1021\/jacs.3c08539","article-title":"Enhancing Prebiotic Phosphorylation and Modulating the Regioselectivity of Nucleosides with Diamidophosphate","volume":"145","author":"Cruz","year":"2023","journal-title":"J. Am. Chem. Soc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1016\/S0273-1177(02)00367-8","article-title":"Prebiotic synthesis of nucleotides at the Earth orbit in presence of Lunar soil","volume":"30","author":"Kuzicheva","year":"2002","journal-title":"Adv. Space Res."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Guo, X., Fu, S., Ying, J., and Zhao, Y. (2023). Prebiotic chemistry: A review of nucleoside phosphorylation and polymerization. Open Biol., 13.","DOI":"10.1098\/rsob.220234"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1038\/2251248a0","article-title":"Non-enzymatic hydrolysis of adenosine phosphates","volume":"225","author":"Hulett","year":"1970","journal-title":"Nature"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Moeller, C., Schmidt, C., Guyot, F., and Wilke, M. (2022). Hydrolysis rate constants of ATP determined in situ at elevated temperatures. Biophys. Chem., 290.","DOI":"10.1016\/j.bpc.2022.106878"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1089\/ast.2019.2045","article-title":"The Hot Spring Hypothesis for an Origin of Life","volume":"20","author":"Damer","year":"2020","journal-title":"Astrobiology"},{"key":"ref_42","first-page":"59","article-title":"An hypothesis concerning the relationship between submarine hot springs and the origin of life on Earth","volume":"4","author":"Corliss","year":"1981","journal-title":"Oceanol. Acta"},{"key":"ref_43","unstructured":"(2025, July 19). Darwin Correspondence Project, \u201cLetter No. 7471\u201d. Available online: www.darwinproject.ac.uk\/DCP-LETT-7471."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.gca.2022.09.027","article-title":"Serpentinization as a route to liberating phosphorus on habitable worlds","volume":"336","author":"Pasek","year":"2022","journal-title":"Geochim. Cosmochim. Acta"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.talanta.2015.12.051","article-title":"Direct and simultaneous quantification of ATP, ADP and AMP by (1)H and (31)P Nuclear Magnetic Resonance spectroscopy","volume":"150","author":"Lian","year":"2016","journal-title":"Talanta"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1038\/s42004-025-01577-0","article-title":"Phosphorylation of nucleosides by P-N bond species generated from prebiotic reduced phosphorus sources","volume":"8","author":"Gull","year":"2025","journal-title":"Commun. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Coulon, R., Papou\u0161kov\u00e1, B., Mohammadi, E., Otyepka, M., Wunnava, S., \u0160poner, J., and \u0160poner, J.E. (2023). Prebiotic Synthesis of 3\u2032,5\u2032-Cyclic Adenosine and Guanosine Monophosphates through Carbodiimide-Assisted Cyclization. Chem. Bio. Chem., 24.","DOI":"10.1002\/cbic.202300510"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e2019GC008734","DOI":"10.1029\/2019GC008734","article-title":"Probable Cold and Alkaline Surface Environment of the Hadean Earth Caused by Impact Ejecta Weathering","volume":"21","author":"Kadoya","year":"2020","journal-title":"Geochem. Geophys. Geosyst."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"Q06014","DOI":"10.1029\/2006GC001449","article-title":"Constraints on Hadean zircon protoliths from oxygen isotopes, Ti-thermometry, and rare earth elements","volume":"8","author":"Trail","year":"2007","journal-title":"Geochem. Geophys. Geosyst."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4619","DOI":"10.1073\/pnas.1702729114","article-title":"Reconstructed ancestral enzymes suggest long-term cooling of Earth\u2019s photic zone since the Archean","volume":"114","author":"Garcia","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"eaax1420","DOI":"10.1126\/sciadv.aax1420","article-title":"The Archean atmosphere","volume":"6","author":"Catling","year":"2020","journal-title":"Sci. Adv."},{"key":"ref_52","unstructured":"Lehninger, A.L., Nelson, D.L., and Cox, M.M. (2005). Lehninger Principles of Biochemistry, Macmillan."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1038\/s41564-018-0163-1","article-title":"Marsarchaeota are an aerobic archaeal lineage abundant in geothermal iron oxide microbial mats","volume":"3","author":"Jay","year":"2018","journal-title":"Nat. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1021\/acsearthspacechem.8b00141","article-title":"A Polyextreme Hydrothermal System Controlled by Iron: The Case of Dallol at the Afar Triangle","volume":"3","author":"Kotopoulou","year":"2019","journal-title":"ACS Earth Space Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/j.apgeochem.2008.12.024","article-title":"Partitioning geochemistry of arsenic and antimony, El Tatio Geyser Field, Chile","volume":"24","author":"Landrum","year":"2009","journal-title":"J. Appl. Geochem."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Semenkov, I.N., Klink, G.V., Lebedeva, M.P., Krupskaya, V.V., Chernov, M.S., Dorzhieva, O.V., Kazinskiy, M.T., Sokolov, V.N., and Zavadskaya, A.V. (2021). The variability of soils and vegetation of hydrothermal fields in the Valley of Geysers at Kamchatka Peninsula. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-90712-7"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0375-6505(92)90066-I","article-title":"Geology of the rotorua geothermal system","volume":"21","author":"Wood","year":"1992","journal-title":"Geothermics"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1007\/s12052-012-0443-9","article-title":"Prebiotic Chemistry: What We Know, What We Don\u2019t","volume":"5","author":"Cleaves","year":"2012","journal-title":"Evo. Edu. Outreach"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1126\/science.161.3836.64","article-title":"Prebiotic synthesis: Phosphorylation in aqueous solution","volume":"161","author":"Lohrmann","year":"1968","journal-title":"Science"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1007\/BF02202102","article-title":"Prebiotic synthesis of diaminopyrimidine and thiocytosine","volume":"43","author":"Robertson","year":"1996","journal-title":"J. Mol. Evol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/BF00926938","article-title":"Prebiotic condensation reactions in an aqueous medium: A review of condensing agents","volume":"7","author":"Hulshof","year":"1976","journal-title":"Origins Life Evol. Biosph."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"19447","DOI":"10.1021\/jacs.2c07774","article-title":"Azoles as Auxiliaries and Intermediates in Prebiotic Nucleoside Synthesis","volume":"144","author":"Ritson","year":"2022","journal-title":"J. Am. Chem. Soc."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Clark, B.C., and Kolb, V.M. (2020). Macrobiont: Cradle for the Origin of Life and Creation of a Biosphere. Life, 10.","DOI":"10.3390\/life10110278"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1002\/anie.201506585","article-title":"The Origin of Life\u2014Out of the Blue","volume":"55","author":"Sutherland","year":"2016","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_65","unstructured":"Faure, G. (1997). Principles and Applications of Geochemistry, Pearson. [2nd ed.]."},{"key":"ref_66","unstructured":"(2025, August 30). 31P NMR of Adenosine Phosphates. Available online: https:\/\/www.aiinmr.com\/wp-content\/uploads\/2020\/06\/31P-of-Adenosine-Phoshates.pdf."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/17\/3587\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:38:01Z","timestamp":1760035081000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/17\/3587"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,2]]},"references-count":66,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["molecules30173587"],"URL":"https:\/\/doi.org\/10.3390\/molecules30173587","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,2]]}}}