{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:47:40Z","timestamp":1785242860732,"version":"3.55.0"},"reference-count":64,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010621","name":"Medical University of Lublin, Poland","doi-asserted-by":"publisher","award":["DS30"],"award-info":[{"award-number":["DS30"]}],"id":[{"id":"10.13039\/501100010621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009687","name":"Wroclaw Medical University, Poland","doi-asserted-by":"publisher","award":["SUBZ.D110.25.050"],"award-info":[{"award-number":["SUBZ.D110.25.050"]}],"id":[{"id":"10.13039\/501100009687","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Kazakh National Medical University, Almaty, Kazakhstan"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Molecules"],"abstract":"<jats:p>Geum aleppicum Jacq. (yellow avens), a species traditionally used in folk medicine, remains understudied in the ethnopharmacological aspects. In this study, we comprehensively evaluated the phytochemical composition and biological activity of a hydroethanolic (50:50, v\/v) extract from the aerial parts of G. aleppicum collected in Kazakhstan. Using the high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-QTOF-MS\/MS), we identified 24 compounds, predominantly phenolic acids, flavonoids, tannins, and triterpenoids. The major compound was ellagic acid (2.28 mg\/g dry extract) as revealed by the reverse phase high-performance liquid chromatography\u2013diode array detector (RP-HPLC-DAD). The extract exhibited a high polyphenol content (131.45 mg GAE\/g) and strong antioxidant activity in Ferric Reducing Antioxidant Power (FRAP) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay (3.82 \u00b1 0.07 mmol Fe2+\/g and 106.61 \u00b1 0.89 mg GAE\/g, respectively). Antimicrobial assay of the extract revealed notable antifungal activity against Candida spp., especially against C. glabrata and C. tropicalis with minimum inhibitory concentration (MIC) of as low as 0.125 mg\/mL, showing fungistatic effect. Although the extract inhibited the cytopathic effect induced by Human Herpesvirus 1 (HHV-1) in VERO cells, it did not significantly reduce viral replication. Moreover, among human cancer cell lines studied, the extract exerted moderate and selective cytotoxicity against A549 lung cancer cells (CC50 = 75.51 \u00b5g\/mL, SI = 9). These findings highlight G. aleppicum as a rich source of bioactive compounds, especially phenolics, supporting its potential for development of pharmaceutical and cosmetic applications.<\/jats:p>","DOI":"10.3390\/molecules30193888","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T06:02:52Z","timestamp":1758866572000},"page":"3888","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Unraveling the Chemical Composition and Biological Activity of Geum aleppicum Jacq.: Insights from Plants Collected in Kazakhstan"],"prefix":"10.3390","volume":"30","author":[{"given":"Gulnur N.","family":"Kuntubek","sequence":"first","affiliation":[{"name":"Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martyna","family":"Kasela","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaldanay K.","family":"Kozhanova","sequence":"additional","affiliation":[{"name":"Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wirginia","family":"Kukula-Koch","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy with Medicinal Plant Garden, Medical University in Lublin, 1 Chodzki Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1626-680X","authenticated-orcid":false,"given":"\u0141ukasz","family":"\u015awi\u0105tek","sequence":"additional","affiliation":[{"name":"Department of Virology with Viral Diagnostics Laboratory, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kinga","family":"Salwa","sequence":"additional","affiliation":[{"name":"Department of Virology with Viral Diagnostics Laboratory, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5795-8605","authenticated-orcid":false,"given":"Piotr","family":"Oki\u0144czyc","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Herbal Medicines, Wroc\u0142aw Medical University, 211a Borowska Street, 50-556 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"J\u00f3zefczyk","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy with Medicinal Plant Garden, Medical University in Lublin, 1 Chodzki Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaros\u0142aw","family":"Widelski","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy with Medicinal Plant Garden, Medical University in Lublin, 1 Chodzki Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulnara M.","family":"Kadyrbayeva","sequence":"additional","affiliation":[{"name":"Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aigerim Z.","family":"Mukhamedsadykova","sequence":"additional","affiliation":[{"name":"Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1400-1971","authenticated-orcid":false,"given":"Zuriyadda B.","family":"Sakipova","sequence":"additional","affiliation":[{"name":"Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1503-7634","authenticated-orcid":false,"given":"Anna","family":"Malm","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bunse, M., Mail\u00e4nder, L.K., Lorenz, P., Stintzing, F.C., and Kammerer, D.R. 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