{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:57:18Z","timestamp":1789664238704,"version":"4.0.1"},"reference-count":38,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:00:00Z","timestamp":1760054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Background: Eleutherococcus senticosus (Rupr. et Maxim.) Maxim., widely used in Russian and Chinese traditional medicine for its anti-inflammatory activity, contains bioactive compounds capable of stabilizing epithelial function and reducing inflammation. Despite prior research on its effects in the colon, the impact and mechanism of action of E. senticosus fruit extract on epithelial tissues of the upper digestive and respiratory tract remains unexplored. Objectives: This study aimed to evaluate the influence of E. senticosus fruit extract on the transepithelial electrical potential and resistance in the tracheal and small intestinal epithelium of rabbits. In addition, the chemical composition of the extract was also profiled by the means of UHPLC-DAD-MS. Methods: Tissue segments from the trachea and small intestine of New Zealand white male rabbits were examined using the Ussing chamber technique. Three concentrations of E. senticosus fruit extract (0.001, 0.1, 10 mg\/100 mL) were applied, and changes in transepithelial electrical potential (dPD) and resistance (R) were recorded. Chemical analysis of the extract was conducted using UHPLC-DAD-MS. Results: For the first time, we have discovered that the E. senticosus extract increased membrane resistance in tracheal tissue, suggesting enhanced barrier integrity. In contrast, a slight decrease in resistance was observed in small intestinal tissue. UHPLC-DAD-MS confirmed the presence of chlorogenic acid, dicaffeoylquinic acids, quercetin derivatives, and myo-inositol, compounds known for their antioxidant, anti-inflammatory, and membrane-stabilizing effects. Conclusions: The differential response of respiratory and intestinal epithelium to the E. senticosus extract highlights its tissue-specific action and supports its traditional use in the prevention and treatment of diseases characterized by epithelial barrier dysfunction, such as asthma, COPD, and Crohn\u2019s disease.<\/jats:p>","DOI":"10.3390\/molecules30204041","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:50:16Z","timestamp":1760107816000},"page":"4041","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Eleutherococcus senticosus Fruit Extract Stimulates the Membrane Potential of the Trachea and Small Intestine in Rabbits"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7474-9338","authenticated-orcid":false,"given":"Filip","family":"Graczyk","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Botany and Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 Marie Curie-Sk\u0142odowska Street, 85-094 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El\u017cbieta","family":"Piskorska","sequence":"additional","affiliation":[{"name":"Department of Pathobiochemistry and Clinical Chemistry, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 Marie Curie-Sk\u0142odowska Street, 85-094 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7131-7051","authenticated-orcid":false,"given":"Dorota","family":"Gawenda-Kempczy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany and Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 Marie Curie-Sk\u0142odowska Street, 85-094 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krystian","family":"Krolik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany and Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 Marie Curie-Sk\u0142odowska Street, 85-094 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2743-4071","authenticated-orcid":false,"given":"Jakub","family":"G\u0119balski","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany and Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 Marie Curie-Sk\u0142odowska Street, 85-094 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9742-451X","authenticated-orcid":false,"given":"Dorota","family":"Olszewska-S\u0142onina","sequence":"additional","affiliation":[{"name":"Department of Pathobiochemistry and Clinical Chemistry, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 Marie Curie-Sk\u0142odowska Street, 85-094 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneta","family":"Kondrzycka-D\u0105da","sequence":"additional","affiliation":[{"name":"University of Social Sciences, 9 Sienkiewicza Str., 90-113 \u0141\u00f3d\u017a, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0466-1343","authenticated-orcid":false,"given":"Magdalena","family":"W\u00f3jciak","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Medical University of Lublin, 4a Chodzki Str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8010-0180","authenticated-orcid":false,"given":"Orazio","family":"Taglialatela-Scafati","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Montesano 49, 80132 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6180-1424","authenticated-orcid":false,"given":"Robert","family":"Verpoorte","sequence":"additional","affiliation":[{"name":"Natural Products Laboratory, Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7821-7002","authenticated-orcid":false,"given":"Daniel","family":"Za\u0142uski","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany and Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 Marie Curie-Sk\u0142odowska Street, 85-094 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.jep.2012.12.040","article-title":"Acanthopanax senticosus Extracts Have a Protective Effect on Drosophila Gut Immunity","volume":"146","author":"Li","year":"2013","journal-title":"J. Ethnopharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"8374295","DOI":"10.1155\/2017\/8374295","article-title":"Phytoconstituents and Nutritional Properties of the Fruits of Eleutherococcus divaricatus and Eleutherococcus sessiliflorus: A Study of Non-European Species Cultivated in Poland","volume":"2017","author":"Olech","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_3","first-page":"1483","article-title":"Bioactive Compounds and Antioxidative, Antileukemic and Anti-MMPs Activity of Eleutherococcus Species Cultivated in Poland","volume":"7","author":"Smolarz","year":"2012","journal-title":"Nat. Prod. Commun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"673145","DOI":"10.1155\/2012\/673145","article-title":"A Herbal Composition of Scutellaria Baicalensis and Eleutherococcus senticosus Shows Potent Anti-Inflammatory Effects in an Ex Vivo Human Mucosal Tissue Model","volume":"2012","author":"Zhang","year":"2012","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1248\/bpb.b19-01027","article-title":"Acanthopanax senticosus Root Extract Exerts Dual Action on Mouse Ileal Smooth Muscle Function, Leading to Modulation of Gastrointestinal Motility","volume":"43","author":"Kagota","year":"2020","journal-title":"Biol. Pharm. Bull."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"117847","DOI":"10.1016\/j.jep.2024.117847","article-title":"The Eleutherococcus senticosus Fruits\u2019 Intractum Affects Changes in the Transepithelial Electric Potential in the Distal Section of the Rabbit\u2019s Large Intestine and Inhibits Hyaluronidase","volume":"325","author":"Graczyk","year":"2024","journal-title":"J. Ethnopharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Panossian, A., and Brendler, T. (2020). The Role of Adaptogens in Prophylaxis and Treatment of Viral Respiratory Infections. Pharmaceuticals, 13.","DOI":"10.3390\/ph13090236"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Zhang, X., Guan, L., Zhu, L., Wang, K., Gao, Y., Li, J., Yan, S., Ji, N., Zhou, Y., and Yao, X. (2024). A Review of the Extraction and Purification Methods, Biological Activities, and Applications of Active Compounds in Acanthopanax senticosus. Front. Nutr., 11.","DOI":"10.3389\/fnut.2024.1391601"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"113636","DOI":"10.1016\/j.jep.2020.113636","article-title":"The Intractum from the Eleutherococcus senticosus Fruits Affects the Innate Immunity in Human Leukocytes: From the Ethnomedicinal Use to Contemporary Evidence-Based Research","volume":"268","author":"Graczyk","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/1749-4877.12788","article-title":"Adaptation in Brain Structure and Respiratory and Olfactory Structures across Environmental Gradients in African and North American Muroid Rodents","volume":"19","author":"Taylor","year":"2024","journal-title":"Integr. Zool."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1111\/1749-4877.12766","article-title":"Physiological Strategies in Wild Rodents: Immune Defenses of Commensal Rats","volume":"19","author":"Mirkov","year":"2024","journal-title":"Integr. Zool."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1801448","DOI":"10.1183\/13993003.01448-2018","article-title":"What Is Early COPD and Why Is It Important?","volume":"52","author":"Soriano","year":"2018","journal-title":"Eur. Respir. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1055\/s-2005-869535","article-title":"Pathogenesis of COPD","volume":"26","author":"Spurzem","year":"2005","journal-title":"Semin. Respir. Crit. Care Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1016\/S0140-6736(23)02586-2","article-title":"Crohn\u2019s Disease","volume":"403","author":"Dolinger","year":"2024","journal-title":"Lancet"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Gryn-Rynko, A., Ho\u0142y\u0144ska-Iwan, I., Janiak, M.A., Olszewska-S\u0142onina, D., Amarowicz, R., and Graczyk, R. (2022). The Impact of Morus alba L. Leaf Extract on Intestinal Ion Transport. An in Vitro Study. Biomed. Pharmacother., 150.","DOI":"10.1016\/j.biopha.2022.112939"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ho\u0142y\u0144ska-Iwan, I., Sobiesiak, M., Kowalczyk, W., Wr\u00f3blewski, M., Cwynar, A., and Szewczyk-Golec, K. (2023). Nickel Ions Influence the Transepithelial Sodium Transport in the Trachea, Intestine and Skin. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-33690-2"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1152\/physrev.00026.2001","article-title":"Electrolyte Transport in the Mammalian Colon: Mechanisms and Implications for Disease","volume":"82","author":"Kunzelmann","year":"2002","journal-title":"Physiol. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4562","DOI":"10.1039\/D1FO02662C","article-title":"Chlorogenic Acid Improves the Intestinal Barrier by Relieving Endoplasmic Reticulum Stress and Inhibiting ROCK\/MLCK Signaling Pathways","volume":"13","author":"Song","year":"2022","journal-title":"Food Funct."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"103949","DOI":"10.1016\/j.psj.2024.103949","article-title":"Protective Effects of Chlorogenic Acid on the Intestinal Barrier of Broiler Chickens: An Immunological Stress Model Study","volume":"103","author":"Zha","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.biopha.2017.10.064","article-title":"Chlorogenic Acid (CGA): A Pharmacological Review and Call for Further Research","volume":"97","author":"Naveed","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.saa.2019.04.025","article-title":"In Vitro Methods to Determine the Antioxidant Activity of Caffeic Acid","volume":"219","author":"Spagnol","year":"2019","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/0891-5849(95)00052-Y","article-title":"Inhibition of Human Low-Density Lipoprotein Oxidation by Caffeic Acid and Other Hydroxycinnamic Acid Derivatives","volume":"19","author":"Nardini","year":"1995","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Chhetri, D.R. (2019). Myo-Inositol and Its Derivatives: Their Emerging Role in the Treatment of Human Diseases. Front. Pharmacol., 10.","DOI":"10.3389\/fphar.2019.01172"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"105524","DOI":"10.1016\/j.jff.2023.105524","article-title":"Expression of Inflammatory Genes, WBC-Derived Inflammatory Biomarkers and Liver Function Indices: Effects of Myo-Inositol Supplementation in Obese Patients with NAFLD","volume":"104","author":"Arefhosseini","year":"2023","journal-title":"J. Funct. Foods"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Arefhosseini, S., Roshanravan, N., Tutunchi, H., Rostami, S., Khoshbaten, M., and Ebrahimi-Mameghani, M. (2023). Myo-Inositol Supplementation Improves Cardiometabolic Factors, Anthropometric Measures, and Liver Function in Obese Patients with Non-Alcoholic Fatty Liver Disease. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1092544"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Quecchia, C., and Vianello, A. (2025). The Therapeutic Potential of Myo-Inositol in Managing Patients with Respiratory Diseases. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26052185"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2915","DOI":"10.1021\/mp400030n","article-title":"The Effects of Mannitol on the Transport of Ciprofloxacin across Respiratory Epithelia","volume":"10","author":"Ong","year":"2013","journal-title":"Mol. Pharm."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1002\/jgm.171","article-title":"Transfection of Epithelial Cells Is Enhanced by Combined Treatment with Mannitol and Polyethyleneglycol","volume":"3","author":"Pengg","year":"2001","journal-title":"J. Gene Med."},{"key":"ref_29","first-page":"82","article-title":"Mannitol: A Review of Its Clinical Uses","volume":"12","author":"Shawkat","year":"2012","journal-title":"Contin. Educ. Anaesth. Crit. Care Pain"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1080\/0886022X.2017.1419973","article-title":"Renal Tubular Epithelial Cells Injury Induced by Mannitol and Its Potential Mechanism","volume":"40","author":"Shi","year":"2018","journal-title":"Ren. Fail."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Miyano, T., Suzuki, A., and Sakamoto, N. (2021). Hyperosmotic Stress Induces Epithelialmesenchymal Transition through Rearrangements of Focal Adhesions in Tubular Epithelial Cells. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0261345"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5511","DOI":"10.1007\/s00253-015-6626-x","article-title":"Identification of Mannitol as Compatible Solute in Gluconobacter oxydans","volume":"99","author":"Zahid","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"312ra177","DOI":"10.1126\/scitranslmed.aad0286","article-title":"Sodium Channel Nax Is a Regulator in Epithelial Sodium Homeostasis","volume":"7","author":"Xu","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3181\/00379727-191-42881","article-title":"Nickel Absorption and Kinetics in Human Volunteers","volume":"191","author":"Sunderman","year":"1989","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"101","DOI":"10.5056\/jnm16027","article-title":"Irritable Bowel Syndrome and Nickel Allergy: What Is the Role of the Low Nickel Diet?","volume":"23","author":"Rizzi","year":"2017","journal-title":"J. Neurogastroenterol. Motil."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Brasier, A.R., Zhao, Y., and Chung, K.F. (2024). Editorial: Mucosal Adaptations to Chronic Airway Injury: Mechanisms and Interrelationships of Epithelial Plasticity on Innate Immunity and Airway Remodeling. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1435120"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1080\/17476348.2025.2492800","article-title":"A Tale as Old as Time\u2014The Importance of Accelerated Lung Aging in Chronic Obstructive Pulmonary Disease","volume":"19","author":"Wang","year":"2025","journal-title":"Expert Rev. Respir. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/S2468-1253(24)00390-X","article-title":"The Intestinal Barrier: A Pivotal Role in Health, Inflammation, and Cancer","volume":"10","author":"Neurath","year":"2025","journal-title":"Lancet Gastroenterol. Hepatol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/20\/4041\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:50:22Z","timestamp":1760107822000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/20\/4041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,10]]},"references-count":38,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["molecules30204041"],"URL":"https:\/\/doi.org\/10.3390\/molecules30204041","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,10]]}}}