{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T18:43:47Z","timestamp":1785782627199,"version":"3.56.0"},"reference-count":56,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:00Z","timestamp":1760140800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Committee of Science of the Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["AP23484420"],"award-info":[{"award-number":["AP23484420"]}]},{"name":"the Committee of Science of the Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["BR21882220"],"award-info":[{"award-number":["BR21882220"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A novel inclusion complex of a fluorinated pyrazolopiperidine derivative (5-benzyl-7-(2-fluorobenzylidene)-2,3-bis(2-fluorophenyl)-3,3a,4,5,6,7-hexahydro-2H-pyrazolo [4,3-c]pyridine hydrochloride, PP\u00b7HCl) with \u03b2-cyclodextrin (PP\u03b2CD) was designed, synthesized, and characterized as a potential therapeutic agent for chemotherapy-induced myelosuppression and lymphopenia. Encapsulation of PP within \u03b2-cyclodextrin increased aqueous solubility by approximately 3.4-fold and improved dissolution rate by 2.8-fold compared with the free compound. Structural analysis using IR, ^1H\/^13C NMR, and TLC confirmed the formation of a stable 1:1 host\u2013guest complex, and the disappearance of free PP signals further supported complete encapsulation. In vivo evaluation in a cyclophosphamide-induced myelosuppression model demonstrated that PP\u03b2CD accelerated hematopoietic recovery, restoring leukocyte and erythrocyte counts 35\u201340% faster than methyluracil, without any signs of systemic toxicity. These findings indicate that \u03b2-cyclodextrin complexation significantly enhances solubility, dissolution, and biological efficacy of the pyrazolopiperidine scaffold, supporting further preclinical development of PP\u03b2CD as a supportive therapy for chemotherapy-related hematological complications.<\/jats:p>","DOI":"10.3390\/molecules30204047","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T06:06:32Z","timestamp":1760508392000},"page":"4047","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Design, Characterization, and Hematopoietic Efficacy of a Fluorinated Pyrazolopiperidine Inclusion Complex"],"prefix":"10.3390","volume":"30","author":[{"given":"Zhanargul","family":"Koshetova","sequence":"first","affiliation":[{"name":"Laboratory of Synthetic and Natural Medicinal Compounds Chemistry, A.B. Bekturov Institute of Chemical Sciences, 106 Sh. 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