{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:14:24Z","timestamp":1791918864761,"version":"4.3.4"},"reference-count":69,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MIUR (Ministry of University and Research), Italy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Polyalthia longifolia, a member of the Annonaceae family, is traditionally used for its medicinal properties, including as an antidiabetic remedy, primarily in Asia and sub-Saharan Africa. This study investigated the potential of six P. longifolia extracts in counteracting hyperglycemia and diabetes-related complications. Aqueous, ethanol, and methanol extracts from leaves and stems were evaluated for their antihyperglycemic, antiglycation, and antiradical properties using \u03b1-glucosidase, BSA, and ORAC assays, respectively. Phytochemical characterization was conducted using TPC and TFC assays, and HPLC analysis identified specific bioactive compounds, including various phenolic compounds (gallic acid, (+)-catechin, epicatechin, caffeic acid, ellagic acid and rosmarinic acid) and flavonoids (luteolin, kaempferol and baicalein). The MTT assay on the human cell line HT-29 assessed the activity of extracts on cell viability, showing slight cytotoxicity. Results demonstrated significant antidiabetic activity of the ethanol and methanol extracts from P. longifolia leaves. This study provides new insights into the potential use of P. longifolia in diabetes mellitus and supports the valorization of traditional medicinal plants.<\/jats:p>","DOI":"10.3390\/molecules30214264","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T16:18:42Z","timestamp":1762186722000},"page":"4264","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring the Antidiabetic Properties of Polyalthia longifolia Leaf and Stem Extracts: In Vitro \u03b1-Glucosidase and Glycation Inhibition"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5936-1789","authenticated-orcid":false,"given":"Guglielmina","family":"Froldi","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1556-332X","authenticated-orcid":false,"given":"Marguerite Kamdem","family":"Simo","sequence":"additional","affiliation":[{"name":"Antimicrobial & Biocontrol Agents Unit, Department of Biochemistry, University of Yaound\u00e9 I, Yaound\u00e9 P.O. Box 812, Cameroon"},{"name":"Department of Biological Sciences, University of Maroua, Maroua P.O. Box 814, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Tomasi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Tadiotto","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4172-1010","authenticated-orcid":false,"given":"Francine Medjiofack","family":"Djeujo","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier Gabriel","family":"Fopokam","sequence":"additional","affiliation":[{"name":"Antimicrobial & Biocontrol Agents Unit, Department of Biochemistry, University of Yaound\u00e9 I, Yaound\u00e9 P.O. 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Box 24 157, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3147-364X","authenticated-orcid":false,"given":"Fabrice","family":"Fekam Boyom","sequence":"additional","affiliation":[{"name":"Antimicrobial & Biocontrol Agents Unit, Department of Biochemistry, University of Yaound\u00e9 I, Yaound\u00e9 P.O. Box 812, Cameroon"},{"name":"Advanced Research & Health Innovation Hub, University of Yaound\u00e9 I, Yaound\u00e9 P.O. Box 337, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"109119","DOI":"10.1016\/j.diabres.2021.109119","article-title":"IDF Diabetes Atlas: Global, Regional and Country-Level Diabetes Prevalence Estimates for 2021 and Projections for 2045","volume":"183","author":"Sun","year":"2022","journal-title":"Diabetes Res. Clin. 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