{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:06:19Z","timestamp":1790910379888,"version":"4.1.0"},"reference-count":97,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study aimed to investigate the phytochemical constituents of C. odorata leaves and stems and to evaluate their antioxidant, total phenol, \u03b1-glucosidase, and antibacterial activities. Furthermore, liquid chromatography-high-resolution mass spectrometry (LC\u2013HRMS)-based metabolite profiling combined with principal component analysis (PCA) was applied to correlate metabolite composition with functional activities, providing comprehensive insights into the metabolomic diversity and bioactive differentiation between plant parts. The plant materials were extracted using 70% and 100% ethanol for 24 h. The leaf extract of ethanol 70% (EtOH 70) exhibited the highest antioxidant activity (IC50 of 223.33 \u00b1 9.20 \u00b5g\/mL) and total phenolic content (113.15 mg GAE\/g), while the stem EtOH 70% extract showed the strongest antidiabetic activity through \u03b1-glucosidase inhibitory activity (78.57%). Although appearing less potent, all extracts showed dose-dependent inhibitory activity, such as Staphylococcus aureus (highest value at 9.31 mm), Escherichia coli (highest value at 9.92 mm), and Salmonella typhimurium (highest value at 9.00 mm). Comparing the plant parts, leaf extracts generally showed more potent activity than stem extracts, particularly evident against E. coli (e.g., Leaf EtOH 70% at 5 mg\/mL: 9.92 mm vs. Stem EtOH 70%: 7.97 mm). LC-HRMS analysis revealed the presence of phenolics, flavonoids, amino acids, organic acids, and alkaloids. Furthermore, the result indicates that C. odorata is a rich source of bioactive compounds with significant antioxidant, \u03b1-glucosidase inhibitory, and antibacterial potency. The findings advance existing knowledge beyond earlier phytochemical or single-activity studies, offering a more holistic understanding of C. odorata\u2019s therapeutic potential and its relevance for natural product development.<\/jats:p>","DOI":"10.3390\/molecules30214314","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T10:08:51Z","timestamp":1762423731000},"page":"4314","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Phytochemical Constituent of Devil Weed (Chromolaena odorata), Concurrent with Its Antioxidant, \u03b1-Glucosidase Inhibitory, and Antibacterial Activity"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7073-2421","authenticated-orcid":false,"given":"Anastasia Wheni","family":"Indrianingsih","sequence":"first","affiliation":[{"name":"Research Center for Food Technology and Processing, National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad F. F.","family":"Ahla","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Universitas Negeri Malang, Malang 65145, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2235-7575","authenticated-orcid":false,"given":"Anjar","family":"Windarsih","sequence":"additional","affiliation":[{"name":"Research Center for Food Technology and Processing, National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1033-8844","authenticated-orcid":false,"family":"Suratno","sequence":"additional","affiliation":[{"name":"Research Center for Food Technology and Processing, National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3951-5987","authenticated-orcid":false,"given":"Tri","family":"Wiyono","sequence":"additional","affiliation":[{"name":"Research Center for Food Technology and Processing, National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0556-4011","authenticated-orcid":false,"given":"Eka","family":"Noviana","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8524-5100","authenticated-orcid":false,"given":"Nurrulhidayah Ahmad","family":"Fadzhillah","sequence":"additional","affiliation":[{"name":"International Institute for Halal Research and Training (INHART), International Islamic University Malaysia (IIUM), Kuala Lumpur 50728, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8855-5710","authenticated-orcid":false,"given":"Ririn Nur","family":"Alfiani","sequence":"additional","affiliation":[{"name":"Research Center for Food Technology and Processing, National Research and Innovation Agency (BRIN), Yogyakarta 55861, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"key":"ref_1","first-page":"1024","article-title":"Allelopathic Effects of Chromolaena odorata (L.) King and Robinson and Mikania Micrantha H.B.K. on Three Selected Weed Species","volume":"8","author":"Sahid","year":"2014","journal-title":"Aust. J. Crop Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.13057\/biodiv\/d200410","article-title":"A New Trophic Interaction between Invasive Weed, Its Biological Control Agent, and Local Insects: A Case Study of Chromolaena odorata","volume":"20","author":"Rizali","year":"2019","journal-title":"Biodivers. J. Biol. Divers."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Juru, V.N., Ndam, L.M., Tatah, B.N., and Fonge, B.A. (2024). Rhizospheric Soil Chemical Properties and Microbial Response to a Gradient of Chromolaena odorata (L) Invasion in the Mount Cameroon Region. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0312199"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Otabor, J.I., Egbon, I., Toews, M.D., and Uyi, O. (2025). The Double-Edged Sword: Local Perspectives on the Spread, Impact, Management, and Uses of the Invasive Chromolaena odorata in Southern Nigeria. Sustainability, 17.","DOI":"10.3390\/su17083514"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/wbm.12286","article-title":"Ecological Insights and Environmental Threats of Invasive Alien Plant Chromolaena odorata: Prospects for Sustainable Management","volume":"24","author":"Rai","year":"2024","journal-title":"Weed Biol. Manag."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1111\/wre.12439","article-title":"Weed or Not a Weed? Density, Perceptions and Management of Chromolaena odorata (Asteraceae) in West Africa: Voices from Ghana","volume":"60","year":"2020","journal-title":"Weed Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"34","DOI":"10.22159\/ajpcr.2018.v11i10.26863","article-title":"A Pharmacognostic and Pharmacological Review on Chromolaena odorata (Siam Weed)","volume":"11","author":"Kanase","year":"2018","journal-title":"Asian J. Pharm. Clin. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.jep.2015.04.057","article-title":"Chromolaena odorata (L.) R.M. King & H. Rob. (Asteraceae) in Sub-Saharan Africa: A Synthesis and Review of Its Medicinal Potential","volume":"183","author":"Omokhua","year":"2016","journal-title":"J. Ethnopharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"s95","DOI":"10.5530\/pj.2018.6s.18","article-title":"Microscopic Leaf Constant Numbers of Chromolaena odorata in Thailand","volume":"10","author":"Pitakpawasutthi","year":"2018","journal-title":"Pharmacogn. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"012022","DOI":"10.1088\/1757-899X\/506\/1\/012022","article-title":"Description of Chromolaena odorata L. R.M King and H. Robinson as Medicinal Plant: A Review","volume":"506","author":"Zahara","year":"2019","journal-title":"IOP Conf. Ser. Mater. Sci. Eng."},{"key":"ref_11","first-page":"1555","article-title":"A New Flavanone from the Leaves of Chromolaena odorata","volume":"10","author":"Emani","year":"2015","journal-title":"Nat. Prod. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/j.fct.2009.12.005","article-title":"Evaluation of Anti-Oxidant Activities and Total Phenolic Content of Chromolaena odorata","volume":"48","author":"Chaudhury","year":"2010","journal-title":"Food Chem. Toxicol."},{"key":"ref_13","first-page":"513","article-title":"Antidiabetic Potential of Chromolaena odorata Leave Extract and Its Effect on Nrf2\/Keap1 Antioxidant Pathway in the Liver of Diabetic-Induced Wistar Rats","volume":"23","author":"Elekofehinti","year":"2023","journal-title":"Adv. Tradit. Med."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Jungers, J.M., Fargione, J.E., Sheaffer, C.C., Wyse, D.L., and Lehman, C. (2013). Energy Potential of Biomass from Conservation Grasslands in Minnesota, USA. PLoS ONE, 8.","DOI":"10.1371\/annotation\/dcd700ef-e870-41d2-bfd1-068aa5b8b717"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1007\/s12155-014-9555-0","article-title":"Harvest Date Effects on Biomass Yield, Moisture Content, Mineral Concentration, and Mineral Export in Switchgrass and Native Polycultures Managed for Bioenergy","volume":"8","author":"Gamble","year":"2015","journal-title":"BioEnergy Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"966","DOI":"10.18520\/cs\/v118\/i6\/966-969","article-title":"Effect of Extractive Content on Fuelwood Characteristics of Certain Woody and Non-Woody Biomass","volume":"118","author":"Kumar","year":"2020","journal-title":"Curr. Sci."},{"key":"ref_17","first-page":"183","article-title":"Simulated Heat Integration Study of Reactive Distillation Column for Ethanol Synthesis","volume":"38","author":"Singh","year":"2019","journal-title":"Iran. J. Chem. Chem. Eng."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.foodhyd.2017.10.022","article-title":"Effect of Ethanol on the Solubilization of Hydrophobic Molecules by Sodium Caseinate","volume":"77","author":"Qu","year":"2018","journal-title":"Food Hydrocoll."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1007\/s12010-012-0013-1","article-title":"Characterization of a Recombinant Flocculent Saccharomyces cerevisiae Strain That Co-Ferments Glucose and Xylose: I. Influence of the Ratio of Glucose\/Xylose on Ethanol Production","volume":"169","author":"Matsushika","year":"2013","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Gulcin, \u0130., and Alwasel, S.H. (2023). DPPH Radical Scavenging Assay. Processes, 11.","DOI":"10.3390\/pr11082248"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.jpba.2005.07.042","article-title":"V Determination of DPPH Free Radical Scavenging Activity by Reversed-Phase HPLC: A Sensitive Screening Method for Polyherbal Formulations","volume":"40","author":"Chandrasekar","year":"2006","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4251","DOI":"10.1021\/jf500180u","article-title":"Re-Evaluation of the 2,2-Diphenyl-1-Picrylhydrazyl Free Radical (DPPH) Assay for Antioxidant Activity","volume":"62","author":"Xie","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"63","DOI":"10.32598\/ijt.14.2.612","article-title":"Antioxidant and Hypolipidemic Effects of Methanolic Root Extract of Chromolaena odorata in Alloxan-Induced Diabetic Rats","volume":"14","author":"Omonije","year":"2020","journal-title":"Iran. J. Toxicol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"183","DOI":"10.3889\/oamjms.2021.5982","article-title":"Evaluation of Antioxidant Activity of Botto-Botto Leaf Fraction (Chromolaena odorata L.) Using Dpph and Abts Methods","volume":"9","author":"Tahir","year":"2021","journal-title":"Open Access Maced. J. Med. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"145","DOI":"10.22159\/ijap.2024.v16s1.31","article-title":"Antioxidant Activity of the Active Fraction of Mangosteen Rind Extract (Garcinia mangostana)","volume":"16","author":"Widayanti","year":"2024","journal-title":"Int. J. Appl. Pharm."},{"key":"ref_26","first-page":"8441","article-title":"Effects of Extraction Technique and Solvent on Phytochemicals, Antioxidant, and Antimicrobial Activities of Cultivated and Wild Rosemary (Rosmarinus officinalis L.) from Taounate Region (Northern Morocco)","volume":"12","author":"Zeroual","year":"2022","journal-title":"Biointerface Res. Appl. Chem."},{"key":"ref_27","first-page":"219","article-title":"Phytocompounds of the Extracts of Four Medicinal Plants of C\u00f4te d\u2019ivoire and Assessment of Their Potential Antioxidant by Thin Layer Chromatography","volume":"24","author":"Konan","year":"2008","journal-title":"Eur. J. Sci. Res."},{"key":"ref_28","first-page":"6689271","article-title":"Phytochemistry, Biological, and Toxicity Study on Aqueous and Methanol Extracts of Chromolaena odorata","volume":"2023","author":"Shrestha","year":"2023","journal-title":"Sci. World J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1453612","DOI":"10.1155\/2019\/1453612","article-title":"A New Flavanone as a Potent Antioxidant Isolated from Chromolaena odorata L. Leaves","volume":"2019","author":"Putri","year":"2019","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7321","DOI":"10.1007\/s13197-015-1820-5","article-title":"Depletion\/Protection of \u03b2-Carotene in Estimating Antioxidant Activity by \u03b2-Carotene Bleaching Assay","volume":"52","author":"Dawidowicz","year":"2015","journal-title":"J. Food Sci. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"8983","DOI":"10.1021\/jf302218g","article-title":"\u03b2-Carotene Assay Revisited. Application to Characterize and Quantify Antioxidant and Prooxidant Activities in a Microplate","volume":"60","author":"Prieto","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6020","DOI":"10.52711\/0974-360X.2024.00914","article-title":"Phytochemical Composition by GC\u2013MS, Invitro Antioxidant, Insilico Chemical Active Compound of Chromolaena odorata L. Weed Extract Targeting EGFR as Anti Lung Cancer","volume":"17","author":"Hardianti","year":"2024","journal-title":"Res. J. Pharm. Technol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/j.lwt.2007.06.010","article-title":"In Vitro Antioxidant Properties of Rutin","volume":"41","author":"Yang","year":"2008","journal-title":"LWT\u2014Food Sci. Technol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Gong, T., Yang, X., Bai, F., Li, D., Zhao, T., Zhang, J., Sun, L., and Guo, Y. (2020). Young Apple Polyphenols as Natural \u03b1-Glucosidase Inhibitors: In Vitro and in Silico Studies. Bioorg. Chem., 96.","DOI":"10.1016\/j.bioorg.2020.103625"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1007\/s11094-024-03057-6","article-title":"Flavonoids from the Leaves of Chromolaena odorata and Their \u03b1-Glucosidase Inhibitory Activity","volume":"57","author":"Giang","year":"2024","journal-title":"Pharm. Chem. J."},{"key":"ref_36","first-page":"772","article-title":"Pharmacological Evaluation of Antidiabetic Activity of Chromolaena odorata Leaves Extract in Streptozotocin-Induced Rats","volume":"11","author":"Yusuf","year":"2020","journal-title":"Syst. Rev. Pharm."},{"key":"ref_37","first-page":"522","article-title":"In Vitro Investigations of Chemical Composition, Antibacterial, Antioxidant, Antidiabetic, and Anti-Inflammatory Activities of Chromolaena odorata Flower Extracts","volume":"46","author":"Tran","year":"2024","journal-title":"Songklanakarin J. Sci. Technol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.biopha.2017.10.001","article-title":"Mechanisms of Antidiabetic Effects of Flavonoid Rutin","volume":"96","author":"Ghorbani","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.cbi.2009.07.018","article-title":"Efficacy of Coumarin on Hepatic Key Enzymes of Glucose Metabolism in Chemical Induced Type 2 Diabetic Rats","volume":"181","author":"Pari","year":"2009","journal-title":"Chem. Biol. Interact."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.bcab.2018.05.014","article-title":"Phytochemical Screening, Free Radical Scavenging and Antimicrobial Potential of Chromolaena odorata Leaf Extracts against Pathogenic Bacterium in Wound Infections\u2014A Multispectrum Perspective","volume":"15","author":"Vijayaraghavan","year":"2018","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"107799","DOI":"10.1016\/j.micpath.2025.107799","article-title":"Antimicrobial Activity of Chromolaena odorata Crude Extracts against Streptococcus suis","volume":"206","author":"Phetburom","year":"2025","journal-title":"Microb. Pathog."},{"key":"ref_42","first-page":"1521","article-title":"In-Silico, in-Vitro Antibacterial Activity and Toxicity Profile of New Quinoline Derivatives","volume":"52","author":"Sambavekar","year":"2013","journal-title":"Indian J. Chem.\u2014Sect. B Org. Med. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1080\/10715762.2018.1456658","article-title":"Depletion of Reactive Oxygen Species Induced by Chlorogenic Acid Triggers Apoptosis-like Death in Escherichia coli","volume":"52","author":"Lee","year":"2018","journal-title":"Free Radic. Res."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Bajaj, H., Kaushal, S., Kaur, V., Panwar, H., Sharma, P., and Jangra, R. (2024). Isolation of Quinic Acid from Dropped Citrus Reticulata Blanco Fruits: Its Derivatization, Antibacterial Potential, Docking Studies, and ADMET Profiling. Front. Chem., 12.","DOI":"10.3389\/fchem.2024.1372560"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.17798\/bitlisfen.1167047","article-title":"Antioxidant and Antimicrobial Capacity of Quinic Acid","volume":"11","author":"Ercan","year":"2022","journal-title":"Bitlis Eren \u00dcniversitesi Fen Bilim. Derg."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ding, Y., Wen, G., Wei, X., Zhou, H., Li, C., Luo, Z., Ou, D., Yang, J., and Song, X. (2024). Antibacterial Activity and Mechanism of Luteolin Isolated from Lophatherum gracile Brongn. against Multidrug-Resistant Escherichia coli. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1430564"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/s00203-005-0063-7","article-title":"Differential Antibacterial Activity of Genistein Arising from Global Inhibition of DNA, RNA and Protein Synthesis in Some Bacterial Strains","volume":"184","author":"Ulanowska","year":"2006","journal-title":"Arch. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"10048","DOI":"10.1080\/10408398.2023.2220031","article-title":"Total (Poly)Phenol Analysis by the Folin-Ciocalteu Assay as an Anti-Inflammatory Biomarker in Biological Samples","volume":"64","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1002\/pca.3494","article-title":"Paper-Based Device for Phenolic Content Determination in Tea Extracts","volume":"36","author":"Silver","year":"2025","journal-title":"Phytochem. Anal."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"131","DOI":"10.18311\/jnr\/2016\/7035","article-title":"Artemia Salina Lethality and Histopathological Studies of Siam Weed, Chromolaena odorata","volume":"16","author":"Sirinthipaporn","year":"2016","journal-title":"J. Nat. Remedies"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Kut, K., Tama, A., Furdak, P., Bartosz, G., and Sadowska-Bartosz, I. (2024). Generation of Hydrogen Peroxide and Phenolic Content in Plant-Material-Based Beverages and Spices. Processes, 12.","DOI":"10.3390\/pr12010166"},{"key":"ref_52","first-page":"377","article-title":"Antioxidant Capacity and Total Phenol Content of Commonly Consumed Selected Vegetables of Bangladesh","volume":"17","author":"Sharmin","year":"2011","journal-title":"Malays. J. Nutr."},{"key":"ref_53","first-page":"49","article-title":"Study of Spectra Physical for Some Samples of Medical Herbal by FTIR-ATR Spectroscopy","volume":"13","author":"Hamza","year":"2022","journal-title":"J. Pharm. Negat. Results"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"137","DOI":"10.7324\/JAPS.2019.90319","article-title":"Application of Near- and Mid-Infrared Spectroscopy Combined with Chemometrics for Discrimination and Authentication of Herbal Products: A Review","volume":"9","author":"Rohman","year":"2019","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1080\/10408347.2020.1820851","article-title":"A Glimpse into the Extraction Methods of Active Compounds from Plants","volume":"52","author":"Chuo","year":"2022","journal-title":"Crit. Rev. Anal. Chem."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Ding, H., Li, Y., Zhao, C., Yang, Y., Xiong, C., Zhang, D., Feng, S., Wu, J., and Wang, X. (2022). Rutin Supplementation Reduces Oxidative Stress, Inflammation and Apoptosis of Mammary Gland in Sheep During the Transition Period. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.907299"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"6241017","DOI":"10.1155\/2018\/6241017","article-title":"Rutin as a Potent Antioxidant: Implications for Neurodegenerative Disorders","volume":"2018","author":"Enogieru","year":"2018","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Alqudah, A., Qnais, E.Y., Wedyan, M.A., Altaber, S., Bseiso, Y., Oqal, M., AbuDalo, R., Alrosan, K., Alrosan, A.Z., and Bani Melhim, S. (2023). Isorhamnetin Reduces Glucose Level, Inflammation, and Oxidative Stress in High-Fat Diet\/Streptozotocin Diabetic Mice Model. Molecules, 28.","DOI":"10.3390\/molecules28020502"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Chunmei, Z., and Shuai, W. (2025). Molecular Mechanisms of Neuroprotective Effect of Rutin. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1599167"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Huang, L., Kim, M.-Y., and Cho, J.Y. (2023). Immunopharmacological Activities of Luteolin in Chronic Diseases. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24032136"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.3892\/etm.2021.10735","article-title":"Effects of Isoflavone Derivatives on the Production of Inflammatory Cytokines by Synovial Cells","volume":"22","author":"Mijiti","year":"2021","journal-title":"Exp. Ther. Med."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Annunziata, F., Pinna, C., Dallavalle, S., Tamborini, L., and Pinto, A. (2020). An Overview of Coumarin as a Versatile and Readily Accessible Scaffold with Broad-Ranging Biological Activities. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21134618"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.3892\/etm.2016.3212","article-title":"Luteolin Potentiates the Effects of Aminoglycoside and \u03b2-Lactam Antibiotics against Methicillin-Resistant Staphylococcus aureus in Vitro","volume":"11","author":"Joung","year":"2016","journal-title":"Exp. Ther. Med."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Morimoto, Y., Aiba, Y., Miyanaga, K., Hishinuma, T., Cui, L., Baba, T., and Hiramatsu, K. (2023). CID12261165, a Flavonoid Compound as Antibacterial Agents against Quinolone-Resistant Staphylococcus aureus. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-28859-8"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Suarez, A.F.L., Tirador, A.D.G., Villorente, Z.M., Bagarinao, C.F., Sollesta, J.V.N., Dumancas, G.G., Sun, Z., Zhan, Z.Q., Saludes, J.P., and Dalisay, D.S. (2021). The Isorhamnetin-Containing Fraction of Philippine Honey Produced by the Stingless Bee Tetragonula Biroi Is an Antibiotic against Multidrug-Resistant Staphylococcus aureus. Molecules, 26.","DOI":"10.3390\/molecules26061688"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.micinf.2003.10.005","article-title":"Phytoestrogen Genistein as an Anti-Staphylococcal Agent","volume":"6","author":"Verdrengh","year":"2004","journal-title":"Microbes Infect."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Yi, L., Bai, Y., Chen, X., Wang, W., Zhang, C., Shang, Z., Zhang, Z., Li, J., Cao, M., and Zhu, Z. (2024). Synergistic Effects and Mechanisms of Action of Rutin with Conventional Antibiotics Against Escherichia coli. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252413684"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Zai, M.J., Cheesman, M.J., and Cock, I.E. (2025). Flavonoids Identified in Terminalia spp. Inhibit Gastrointestinal Pathogens and Potentiate Conventional Antibiotics via Efflux Pump Inhibition. Molecules, 30.","DOI":"10.3390\/molecules30112300"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s12937-020-00586-0","article-title":"Daily Consumption of One Teaspoon of Trehalose Can Help Maintain Glucose Homeostasis: A Double-Blind, Randomized Controlled Trial Conducted in Healthy Volunteers","volume":"19","author":"Yoshizane","year":"2020","journal-title":"Nutr. J."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"9680508","DOI":"10.1155\/2020\/9680508","article-title":"Use of Chlorogenic Acid against Diabetes Mellitus and Its Complications","volume":"2020","author":"Yan","year":"2020","journal-title":"J. Immunol. Res."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Cai, C., Cheng, W., Shi, T., Liao, Y., Zhou, M., and Liao, Z. (2023). Rutin Alleviates Colon Lesions and Regulates Gut Microbiota in Diabetic Mice. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-31647-z"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1007\/s11655-024-3917-z","article-title":"Therapeutic Potential of Luteolin for Diabetes Mellitus and Its Complications","volume":"31","author":"Chang","year":"2025","journal-title":"Chin. J. Integr. Med."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.2174\/1871530323666230516103420","article-title":"Antidiabetic Effects of Genistein: Mechanism of Action","volume":"23","author":"Abbasi","year":"2023","journal-title":"Endocr. Metab. Immune Disord. Drug Targets"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Li, Y., Zheng, L., Chen, M., Li, R., Yu, Y., Qiao, L., Liu, J., Zhang, X., Zhang, Y., and Zhang, Y. (2025). Nootkatone Alleviates Type 2 Diabetes in Db\/Db Mice Through AMPK Activation and ERK Inhibition: An Integrated In Vitro and In Vivo Study. Molecules, 30.","DOI":"10.20944\/preprints202503.1957.v1"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"e1900978","DOI":"10.1002\/mnfr.201900978","article-title":"Flavone Hispidulin Stimulates Glucagon-Like Peptide-1 Secretion and Ameliorates Hyperglycemia in Streptozotocin-Induced Diabetic Mice","volume":"64","author":"Wang","year":"2020","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1093\/jn\/135.9.2103","article-title":"Isoleucine, a Blood Glucose-Lowering Amino Acid, Increases Glucose Uptake in Rat Skeletal Muscle in the Absence of Increases in AMP-Activated Protein Kinase Activity","volume":"135","author":"Doi","year":"2005","journal-title":"J. Nutr."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/j.jff.2015.06.018","article-title":"Phenolics and Polyphenolics in Foods, Beverages and Spices: Antioxidant Activity and Health Effects\u2014A Review","volume":"18","author":"Shahidi","year":"2015","journal-title":"J. Funct. Foods"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"135078","DOI":"10.1016\/j.foodchem.2022.135078","article-title":"Comparing the Antioxidant Activity of Gallic Acid and Its Alkyl Esters in Emulsion Gel and Non-Gelled Emulsion","volume":"407","author":"Keramat","year":"2023","journal-title":"Food Chem."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jsps.2016.04.025","article-title":"The Pharmacological Potential of Rutin","volume":"25","author":"Ganeshpurkar","year":"2017","journal-title":"Saudi Pharm. J."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1002\/ptr.5256","article-title":"Antioxidant and Anti-Inflammatory Properties of the Citrus Flavonoids Hesperidin and Hesperetin: An Updated Review of Their Molecular Mechanisms and Experimental Models","volume":"29","author":"Parhiz","year":"2015","journal-title":"Phytother. Res."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"e26701","DOI":"10.1016\/j.heliyon.2024.e26701","article-title":"Revisiting Luteolin: An Updated Review on Its Anticancer Potential","volume":"10","author":"Rauf","year":"2024","journal-title":"Heliyon"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nri3141","article-title":"How Do Plants Achieve Immunity? Defence without Specialized Immune Cells","volume":"12","author":"Spoel","year":"2012","journal-title":"Nat. Rev. Immunol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1080\/1040869059096","article-title":"Dietary Polyphenols and the Prevention of Diseases","volume":"45","author":"Scalbert","year":"2005","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.jfda.2013.11.001","article-title":"Effect of Extraction Solvent on Total Phenol Content, Total Flavonoid Content, and Antioxidant Activity of Limnophila aromatica","volume":"22","author":"Do","year":"2014","journal-title":"J. Food Drug Anal."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"7313","DOI":"10.3390\/molecules15107313","article-title":"Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties","volume":"15","author":"Dai","year":"2010","journal-title":"Molecules"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.foodchem.2005.07.042","article-title":"Phenolic Compounds in Plants and Agri-Industrial by-Products: Antioxidant Activity, Occurrence, and Potential Uses","volume":"99","author":"Balasundram","year":"2006","journal-title":"Food Chem."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"270","DOI":"10.4161\/oxim.2.5.9498","article-title":"Plant Polyphenols as Dietary Antioxidants in Human Health and Disease","volume":"2","author":"Pandey","year":"2009","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1023\/A:1013713905833","article-title":"Metabolomics--the Link between Genotypes and Phenotypes","volume":"48","author":"Fiehn","year":"2002","journal-title":"Plant Mol. Biol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1002\/mas.21449","article-title":"Mass Spectrometry-Based Plant Metabolomics: Metabolite Responses to Abiotic Stress","volume":"35","author":"Jorge","year":"2016","journal-title":"Mass Spectrom. Rev."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1039\/B906712B","article-title":"Systems Level Studies of Mammalian Metabolomes: The Roles of Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy","volume":"40","author":"Dunn","year":"2011","journal-title":"Chem. Soc. Rev."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.pbi.2013.02.009","article-title":"Plant Sphingolipids: Function Follows Form","volume":"16","author":"Markham","year":"2013","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Sun, S., Yu, Y., Jo, Y., Han, J.H., Xue, Y., Cho, M., Bae, S.-J., Ryu, D., Park, W., and Ha, K.-T. (2025). Impact of Extraction Techniques on Phytochemical Composition and Bioactivity of Natural Product Mixtures. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1615338"},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Windarsih, A., Indrianingsih, A.W., Suryani, R., Wiyono, T., Noviana, E., Darsih, C., Rahayu, E., Pangestuti, R., Asari, S.M., and Pratiwi, S.I. (2025). Evaluation of the Antioxidant, Antidiabetic, and Antibacterial Activity of Curcuma domestica, Zingiber officinale, Alpinia galanga, Curcuma xanthorrhiza, and Kaempferia galanga Concurrent with Its Metabolite\u2019s Constituent Analysis. Chem. Biodivers., 22.","DOI":"10.1002\/cbdv.202500645"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.procbio.2024.04.034","article-title":"The Effect of Extraction Method on Biological Activity and Phytochemical Content of Artocarpus heterophyllus (Jackfruit) Leaves Extract Concurrent with Its Principal Component Analysis","volume":"143","author":"Indrianingsih","year":"2024","journal-title":"Process Biochem."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"4711","DOI":"10.1007\/s12649-025-02925-w","article-title":"In Vitro Evaluation of Antioxidant, Antibacterial, and Antidiabetes of Billygoat Weed Leaves, Stem, and Flower (Ageratum conyzoides L.) Concurrent with Its Phytochemical Constituents","volume":"16","author":"Windarsih","year":"2025","journal-title":"Waste Biomass Valorization"},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Indrianingsih, A.W., Hayati, S.N., Rosyida, V.T., Apriyana, W., Darsih, C., Nisa, K., Suryani, A.E., Wiyono, T., Windarsih, A., and Handayani, S. (2025). Improving the Functional Benefits of Powdered Ginger Beverage through the Incorporation of Ganoderma lucidum. Bioact. Carbohydrates Diet. Fibre, 34.","DOI":"10.1016\/j.bcdf.2025.100482"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"e202333","DOI":"10.25186\/.v20i.2333","article-title":"Phenolic Content and \u03b1-Glucosidase Inhibition of Tubruk-Brew Kalosi Coffee Processed by Different Post-Harvest Processing","volume":"20","author":"Yulianti","year":"2025","journal-title":"Coffee Sci."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/21\/4314\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T10:09:25Z","timestamp":1762423765000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/21\/4314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,6]]},"references-count":97,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["molecules30214314"],"URL":"https:\/\/doi.org\/10.3390\/molecules30214314","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,6]]}}}