{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T04:06:12Z","timestamp":1779163572849,"version":"3.51.4"},"reference-count":27,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ICGEB"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Euryops floribundus has traditionally been recognized for its therapeutic benefits, although its pharmacological potential has not been fully explored. This study investigated the leaves of E. floribundus for volatile compounds, in vitro anticancer potential, antioxidant activity, and phytochemical composition using standard methods. Terpenoids, flavonoids, glycosides, tannins, saponins, and steroids were identified using qualitative screening. FTIR analysis verified that the functional groups corresponded to bioactive substances. Methanol, ethanol, and aqueous extracts showed dose-dependent 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Nitric oxide scavenging activity ranged from 7.2% to 22.5% at 2500 \u03bcg\/mL. GC-MS profiling of essential oils revealed monoterpenes as the primary constituents (60%), with sabinene (27.86%) and terpinen-4-ol (13.63%) as major chemicals. Significant antiproliferative effects were shown by ethanol and methanol extracts against DU-145, PC-3, and SK-UT-1 cancer cell lines with cell viability inhibition ranging from 82.6% to 85.6%, and IC50 values ranging from 1.7 \u00b1 0.02 to 2.7 \u00b1 0.03 \u00b5g\/mL. These findings support the potential therapeutic uses of E. floribundus leaves and necessitate additional bioassay-guided research since they indicate that the leaves are rich in bioactive phytochemicals with antioxidant and anticancer activities.<\/jats:p>","DOI":"10.3390\/molecules30234555","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T11:43:22Z","timestamp":1764157402000},"page":"4555","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Composition of Volatiles, Phytochemical Analysis, Antioxidant and Anticancer Activity of Euryops floribundus Ne.Br. Leaves (Asteraceae)"],"prefix":"10.3390","volume":"30","author":[{"given":"Zoleka","family":"Mhinana","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Biological Sciences, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buyisile","family":"Mayekiso","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biological Sciences, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7140-8327","authenticated-orcid":false,"given":"Siphamandla","family":"Lamula","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biological Sciences, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa"},{"name":"Infectious Diseases and Medicinal Plants Research Niche Area, Department of Biotechnology and Biological Sciences, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0613-2808","authenticated-orcid":false,"given":"Thando","family":"Bhanisa","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biological Sciences, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa"},{"name":"Infectious Diseases and Medicinal Plants Research Niche Area, Department of Biotechnology and Biological Sciences, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0527-1750","authenticated-orcid":false,"given":"Martha","family":"Wium","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology (ICGEB), Weirner & Beit Building, Anzio Rd, Observatory, Cape Town 7935, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1086-4588","authenticated-orcid":false,"given":"Juliano","family":"Paccez","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology (ICGEB), Weirner & Beit Building, Anzio Rd, Observatory, Cape Town 7935, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0736-9508","authenticated-orcid":false,"given":"Luiz","family":"Zerbini","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology (ICGEB), Weirner & Beit Building, Anzio Rd, Observatory, Cape Town 7935, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9031-1117","authenticated-orcid":false,"given":"Callistus","family":"Bvenura","sequence":"additional","affiliation":[{"name":"Horticultural Sciences Department, Faculty of Applied Sciences, Cape Peninsula University of Technology, Symphony Way, Bellville, Cape Town 7535, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1592-9459","authenticated-orcid":false,"given":"Lisa","family":"Buwa-Komoreng","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biological Sciences, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa"},{"name":"Infectious Diseases and Medicinal Plants Research Niche Area, Department of Biotechnology and Biological Sciences, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"685","DOI":"10.5897\/AJB2005.000-3127","article-title":"Phytochemical Constituents of Some Nigerian Medicinal Plants","volume":"4","author":"Edeoga","year":"2005","journal-title":"Afr. 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