{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T22:40:46Z","timestamp":1782427246625,"version":"3.54.5"},"reference-count":58,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Research, Development and Youth of the Slovak Republic"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Prenylated flavonoids from hops (Humulus lupulus L.) represent a distinctive class of bioactive compounds with notable antioxidant and health-promoting properties. This study investigated the extraction behavior and quantitative profiles of three major prenylflavonoids\u2014xanthohumol (XN), isoxanthohumol (IXN), and 8-prenylnaringenin (8-PN)\u2014under varying solvent polarity (ethanol vs. methanol), extraction temperature (50\u2013200 \u00b0C), and sample pretreatment (mechanical vs. cryogenic homogenization). Extractions were performed using accelerated solvent extraction (ASE), and compounds were quantified by HPLC-DAD. Ethanol exhibited higher extraction efficiency than methanol, while cryogenic pretreatment markedly enhanced the release of all target analytes. The maximum recovery was achieved at 150 \u00b0C for XN, 200 \u00b0C for IXN, and 100 \u00b0C for 8-PN. Multifactor statistical analysis (MANCOVA, ANOVA) confirmed significant effects of solvent, temperature, and pretreatment, as well as their interactions (p &lt; 0.001). The combination of ASE and cryogenic homogenization enables efficient isolation and precise quantification of prenylated flavonoids from hops, providing a valuable analytical framework for the development of standardized hop extracts and bioactive formulations.<\/jats:p>","DOI":"10.3390\/molecules30244743","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T08:30:17Z","timestamp":1765528217000},"page":"4743","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Extraction Behavior and Quantitative Profiling of Prenylated Flavonoids from Hops (Humulus lupulus L.) Under Varying Solvent Polarity, Temperature, and Cryogenic Pretreatment"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8201-1692","authenticated-orcid":false,"given":"Nora","family":"Haring","sequence":"first","affiliation":[{"name":"Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4653-0683","authenticated-orcid":false,"given":"Milan","family":"Ch\u0148apek","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6709-9942","authenticated-orcid":false,"given":"Bla\u017eena","family":"Dr\u00e1bov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Xanthohumol: Recent Advances on Resources, Biosynthesis, Bioavailability and Pharmacology","volume":"1","author":"Awouafack","year":"2023","journal-title":"Handb. Diet. 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