{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T03:08:37Z","timestamp":1787368117081,"version":"build-2736575974"},"reference-count":67,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"I\u011fd\u0131r University Scientific Research Projects Coordinatorship","award":["TBY0624Y22"],"award-info":[{"award-number":["TBY0624Y22"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The polyphenol oxidase (PPO) enzyme leads to undesirable consequences by causing enzymatic browning during the processing of vegetables and fruits. As these browning reactions occur, many phenolic compounds of PPO can lead to significant changes in active metabolites due to substrate utilization. This may cause a loss of appearance and nutritional and commercial value of food. The sickleweed (Falcaria vulgaris Bernh.) plant studied in the current research is considered an edible and medicinal food. In the present research, polyphenol oxidase was purified 15.65-fold with a yield of 23.61% by affinity chromatography. The optimum pH and temperature for catechol, 4-methylcatechol, and 3,4-dihydroxyphenylpropionic acid substrates were determined in separate experiments. For all three substrates, the optimum pH was 7.0, while the optimum temperature was 20 \u00b0C. The catalytic efficiency ratio (Vmax\/Km) was employed to assess the substrate specificity. Since the highest Vmax\/Km ratio reflects the greatest substrate affinity, 4-methylcatechol was identified as the substrate with the highest affinity for sickleweed PPO based on these values. pH stability and thermal stability were examined in the presence of 4-methylcatechol. The inhibitory effects of widely used antibrowning agents, sodium metabisulphite, citric acid, and ascorbic acid, on PPO activity were investigated. The results show that ascorbic acid was the most efficient inhibitor.<\/jats:p>","DOI":"10.3390\/molecules30244806","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T10:41:23Z","timestamp":1765968083000},"page":"4806","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Purification and Biochemical Characterization of Polyphenol Oxidase from Falcaria vulgaris Bernh."],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7615-6101","authenticated-orcid":false,"given":"Ceylan Buse","family":"Atlas Okut","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Science and Art, I\u011fd\u0131r University, 76000 I\u011fd\u0131r, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2195-9435","authenticated-orcid":false,"given":"Ay\u015fe","family":"T\u00fcrkhan","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Chemical Processing Technologies, Vocational School of Technical Sciences, I\u011fd\u0131r University, 76000 I\u011fd\u0131r, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"ref_1","first-page":"3568","article-title":"Traditional knowledge of wild edible plants of Igdir Province (East Anatolia, Turkey)","volume":"86","author":"Cakir","year":"2017","journal-title":"Acta Soc. 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