{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:15:26Z","timestamp":1791317726163,"version":"4.3.1"},"reference-count":46,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Guangxi Key Research and Development Plan","award":["AB21220065"],"award-info":[{"award-number":["AB21220065"]}]},{"name":"the counterpart aid project for discipline construction from Guangxi University","award":["2023B04"],"award-info":[{"award-number":["2023B04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Selenium-enriched oyster proteins were hydrolyzed using trypsin to obtain peptides with angiotensin-I-converting enzyme (ACE) inhibitory activity. The hydrolysate was purified by ultrafiltration and two-step reversed-phase high-performance liquid chromatography (RP-HPLC), yielding the most active fraction M4-2 (selenium content: 37.00 \u00b1 0.56 mg\/kg; IC50: 0.774 mg\/mL, significantly lower than the IC50 of the crude hydrolysate, 2.801 mg\/mL). This fraction was further analyzed by LC-MS\/MS and molecular docking, leading to the identification of 91 selenium-containing peptide sequences. Two novel peptides, SeMFRTSSK and QASeMNEATGGK, showing strong binding affinities (\u22129.8 and \u22129.0 kcal\/mol, respectively), were selected. Molecular docking revealed that SeMFRTSSK bound to key residues in the ACE active pocket via hydrogen bonds, whereas QASeMNEATGGK interacted with the Zn2+ active center. Cellular assays using EA.hy926 cells demonstrated that both peptides were non-cytotoxic at concentrations up to 0.25 mg\/mL. At 0.025 mg\/mL, SeMFRTSSK and QASeMNEATGGK enhanced cellular NO release by 202.65% and 273.45%, respectively, while suppressing Endothelin-1 (ET-1) secretion by 18.03% and 27.86%, compared to the blank control group. Notably, these peptides induced higher levels of NO release and greater suppression of ET-1 secretion than those in the captopril-treated positive control group. These findings support selenium-enriched oyster-derived peptides as potential natural antihypertensive ingredients.<\/jats:p>","DOI":"10.3390\/molecules30244818","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T11:08:28Z","timestamp":1766056108000},"page":"4818","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification, Molecular Docking Mechanism and Cellular Activity of Selenium-Enriched ACE Inhibitory Peptides from Oysters"],"prefix":"10.3390","volume":"30","author":[{"given":"Zhuangzhuang","family":"Yue","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Green Chemical Materials and Safety Technology, School of Petroleum and Chemical Engineering, Beibu Gulf University, Qinzhou 535011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Xia","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Xu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Green Chemical Materials and Safety Technology, School of Petroleum and Chemical Engineering, Beibu Gulf University, Qinzhou 535011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingbing","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shufei","family":"Jiao","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Green Chemical Materials and Safety Technology, School of Petroleum and Chemical Engineering, Beibu Gulf University, Qinzhou 535011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingtang","family":"Liang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Green Chemical Materials and Safety Technology, School of Petroleum and Chemical Engineering, Beibu Gulf University, Qinzhou 535011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanzhen","family":"Yin","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Green Chemical Materials and Safety Technology, School of Petroleum and Chemical Engineering, Beibu Gulf University, Qinzhou 535011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyin","family":"Miao","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Han, R., Tian, J., Han, Y., Wang, G., Zhou, G., Dai, C., and Wang, C. 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