{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:33:28Z","timestamp":1787762008107,"version":"build-2784847793"},"reference-count":32,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["BR24992760"],"award-info":[{"award-number":["BR24992760"]}]},{"name":"Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["AP19576946"],"award-info":[{"award-number":["AP19576946"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A new iodine\u2013dextrin\u2013lithium complex (IDLC) was synthesized and structurally characterized as a hybrid supramolecular system combining antiseptic, stabilizing, and biocompatible components. The compound integrates iodine as the primary antimicrobial agent, lithium as a coordination and stabilization element, and dextrin as a biodegradable polysaccharide matrix enabling sustained release. Physicochemical analyses confirmed the formation of a uniform, thermally stable complex. Biological evaluation revealed strong bactericidal activity, with minimum bactericidal concentrations (MBCs) ranging from 1.95 to 15.63 \u00b5g mL\u22121 against both Gram-positive and Gram-negative pathogens, including multidrug-resistant Staphylococcus aureus and Acinetobacter baumannii. Cytotoxicity studies revealed moderate, concentration-dependent effects on human peripheral blood mononuclear cells (CC50 = 0.23\u20130.48 mg\/mL; 11.7\u201324.4 \u03bcg I\/mL) and low toxicity toward MDCK cells (CC50 = 10\u201320 mg\/mL; 507\u20131014 \u03bcg I\/mL), confirming a favorable safety profile. IDLC exhibited cytotoxic effects on tumor cell lines (HepG2, HeLa, AGS, K562, and H9) as well as on the normal MeT-5A cell line; however, the CC50 values are similar, and selectivity indices are close to 1, indicating no selective cytotoxicity toward tumor cells. Thus, IDLC demonstrates non-specific cytotoxicity at high concentrations, consistent with its iodine content. The research confirms that iodine can be effectively stabilized within a dextrin-lithium framework to yield a biologically active, thermally resistant complex, suitable for pharmaceutical use.<\/jats:p>","DOI":"10.3390\/molecules30244822","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T14:32:03Z","timestamp":1766068323000},"page":"4822","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Iodine\u2013Dextrin\u2013Lithium Complex: Morphology, Antibacterial Activity, and Cytotoxicity"],"prefix":"10.3390","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0471-0587","authenticated-orcid":false,"given":"Daniil","family":"Shepilov","sequence":"first","affiliation":[{"name":"JSC Scientific Center for Anti-Infectious Drugs, Almaty 050060, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9621-3534","authenticated-orcid":false,"given":"Seitzhan","family":"Turganbay","sequence":"additional","affiliation":[{"name":"JSC Scientific Center for Anti-Infectious Drugs, Almaty 050060, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8610-7321","authenticated-orcid":false,"given":"Ardak","family":"Jumagaziyeva","sequence":"additional","affiliation":[{"name":"JSC Scientific Center for Anti-Infectious Drugs, Almaty 050060, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3100-6523","authenticated-orcid":false,"given":"Tamara","family":"Bukeyeva","sequence":"additional","affiliation":[{"name":"JSC Scientific Center for Anti-Infectious Drugs, Almaty 050060, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Askarova","sequence":"additional","affiliation":[{"name":"JSC Scientific Center for Anti-Infectious Drugs, Almaty 050060, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didar","family":"Bolatova","sequence":"additional","affiliation":[{"name":"Departement of Science, International IT University, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3454-8889","authenticated-orcid":false,"given":"Yerlan","family":"Doszhanov","sequence":"additional","affiliation":[{"name":"UNESCO Chair in Sustainable Development, Al-Farabi Kazakh National University, Al-Farabi Ave., 71, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6256-9814","authenticated-orcid":false,"given":"Arman","family":"Zhumazhanov","sequence":"additional","affiliation":[{"name":"UNESCO Chair in Sustainable Development, Al-Farabi Kazakh National University, Al-Farabi Ave., 71, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8048-4970","authenticated-orcid":false,"given":"Almagul","family":"Kerimkulova","sequence":"additional","affiliation":[{"name":"Department of Material Sciences, Nanotechnology and Engineering Physics, Satbayev University, Satbayev Str. 22a, Almaty 050013, Kazakhstan"},{"name":"Institute of Combustion Problems, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Saurykova","sequence":"additional","affiliation":[{"name":"Institute of Combustion Problems, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3677-8685","authenticated-orcid":false,"given":"Aitugan","family":"Sabitov","sequence":"additional","affiliation":[{"name":"Institute of Combustion Problems, Almaty 050012, Kazakhstan"},{"name":"Faculty of Natural Sciences, Kazakh National Women\u2019s Teacher Training University, Almaty 050000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e0104724","DOI":"10.1128\/spectrum.01047-24","article-title":"Exploring Antimicrobial Interactions between Metal Ions and Quaternary Ammonium Compounds toward Synergistic Metallo-Antimicrobial Formulations","volume":"12","author":"Lekhan","year":"2024","journal-title":"Microbiol. Spectr."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Babalska, Z.\u0141., Korbecka-Paczkowska, M., and Karpi\u0144ski, T.M. (2021). Wound Antiseptics and European Guidelines for Antiseptic Application in Wound Treatment. Pharmaceuticals, 14.","DOI":"10.3390\/ph14121253"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"103427","DOI":"10.1016\/j.jddst.2022.103427","article-title":"Povidone-Iodine Loaded Bigels: Characterization and Effect as a Hand Antiseptic Agent","volume":"72","author":"Vergara","year":"2022","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/S0140-6736(89)90903-3","article-title":"Topical Iodine-Containing Antiseptics and Neonatal Hypothyroidism in Very-Low-Birthweight Infants","volume":"2","author":"Smerdely","year":"1989","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"107263","DOI":"10.1016\/j.aquatox.2025.107263","article-title":"Lithium Enhanced Plasmid-Mediated Conjugative Transfer of Antimicrobial Resistance Genes in Escherichia coli: Different Concentrations and Mechanisms","volume":"279","author":"Li","year":"2025","journal-title":"Aquat. Toxicol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1089\/ind.2021.0006","article-title":"Influence of Lithium Salt on Escherichia coli Growth and Viability","volume":"18","author":"Chernova","year":"2022","journal-title":"Ind. Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5911","DOI":"10.1038\/s41598-017-05976-9","article-title":"Antimicrobial Efficacy and Synergy of Metal Ions against Enterococcus faecium, Klebsiella pneumoniae and Acinetobacter baumannii in Planktonic and Biofilm Phenotypes","volume":"7","author":"Vaidya","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.2174\/1389201024666230302153849","article-title":"Lithium and Microorganisms: Biological Effects and Mechanisms","volume":"24","author":"Plotnikov","year":"2023","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"121698","DOI":"10.1016\/j.carbpol.2023.121698","article-title":"A Novel Cationic \u03b2-Cyclodextrin Decorated with a Choline-Like Pendant Exhibits Iodophor, Mucoadhesive and Bactericidal Properties","volume":"328","author":"Pedotti","year":"2024","journal-title":"Carbohydr. Polym."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1007\/s13346-024-01586-x","article-title":"Cyclodextrins and Their Potential Applications for Delivering Vitamins, Iron, and Iodine for Improving Micronutrient Status","volume":"15","author":"Saffarionpour","year":"2025","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"107569","DOI":"10.1016\/j.foodhyd.2022.107569","article-title":"Study of Dextrin Addition on the Formation and Physicochemical Properties of Whey Protein-Stabilized Emulsion: Effect of Dextrin Molecular Dimension","volume":"128","author":"Hu","year":"2022","journal-title":"Food Hydrocoll."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.jhin.2017.07.013","article-title":"Iodine-Lithium-Alpha-Dextrin (IL\u03b1D) against Staphylococcus aureus Skin Infections: A Comparative Study of In-Vitro Bactericidal Activity and Cytotoxicity between IL\u03b1D and Povidone-Iodine","volume":"98","author":"Zisi","year":"2018","journal-title":"J. Hosp. Infect."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"746","DOI":"10.3390\/quantum3040047","article-title":"Structure of the Active Nanocomplex of Antiviral and Anti-Infectious Iodine-Containing Drug FS-1","volume":"3","author":"Yuldasheva","year":"2021","journal-title":"Quantum Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1080\/01442350903017302","article-title":"Structure, spectroscopy and dynamics of halogen molecules interacting with water","volume":"28","author":"Kerenskaya","year":"2009","journal-title":"Int. Rev. Phys. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5739","DOI":"10.1002\/ejoc.201700969","article-title":"Comparing strong and weak halogen bonding in solution: 13C NMR, UV\/Vis, crystallographic, and computational studies of an intramolecular model","volume":"2017","author":"Widner","year":"2017","journal-title":"Eur. J. Org. Chem."},{"key":"ref_16","first-page":"1595","article-title":"Dextrin\/Polyvinyl Alcohol\/Iodine Complexes: Preparation, Characterization, and Antibacterial, Cytotoxic Activity","volume":"35","author":"Turganbay","year":"2025","journal-title":"Eng. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"17957","DOI":"10.1021\/acsomega.8b02136","article-title":"A Solution NMR Approach to Determine the Chemical Structures of Carbohydrates Using the Hydroxyl Groups as Starting Points","volume":"3","author":"Brown","year":"2018","journal-title":"ACS Omega"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2248","DOI":"10.1002\/ejic.202100224","article-title":"Amino\u2013Organolithium Compounds and Their Aggregation for the Synthesis of Amino\u2013Organoaluminium Compounds","volume":"23","author":"Bodach","year":"2021","journal-title":"Eur. J. Inorg. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"15778","DOI":"10.1021\/la403526w","article-title":"Before and Beyond the Micellization of n-Alkyl Glycosides: A Water-1H NMR Relaxation Study","volume":"29","author":"Cardoso","year":"2013","journal-title":"Langmuir"},{"key":"ref_20","unstructured":"Council of Europe (2011). European Pharmacopoeia, Council of Europe. [7th ed.]."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/j.cmi.2024.10.001","article-title":"Preclinical and Clinical Studies in the Drug Development Process of European Medicines Agency-Approved Non-HIV Antiviral Agents: A Narrative Review","volume":"31","author":"Pracher","year":"2025","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_22","unstructured":"(1979). Reagents. Iodine. Specifications (Standard No. GOST 4159-79)."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"075602","DOI":"10.1088\/1054-660X\/25\/7\/075602","article-title":"Study of molecular iodine, iodate ions, iodide ions, and triiodide ions solutions absorption in the UV and visible light spectral bands","volume":"25","author":"Kireev","year":"2015","journal-title":"Laser Physics"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Sabitov, A., Turganbay, S., Kerimkulova, A., Doszhanov, Y., Saurykova, K., Atamanov, M., Zhumazhanov, A., and Bolatova, D. (2025). 1-Carboxy-2-phenylethan-1-aminium Iodide 2-Azaniumyl-3-phenylpropanoate Crystals: Properties and Its Biochar-Based Application for Iodine Enrichment of Parsley. Appl. Sci., 15.","DOI":"10.20944\/preprints202509.1225.v1"},{"key":"ref_25","unstructured":"Freshney, R.I. (2015). Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, John Wiley & Sons. [7th ed.]."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1186\/s13756-022-01178-9","article-title":"Effect of povidone-iodine and propanol-based mecetronium ethyl sulphate on antimicrobial resistance and virulence in Staphylococcus aureus","volume":"11","author":"Barakat","year":"2022","journal-title":"Antimicrob. Resist. Infect. Control."},{"key":"ref_28","first-page":"73","article-title":"The in vitro efficacy of neutral electrolysed water and povidone-iodine against CRS-associated biofilms","volume":"60","author":"Lux","year":"2022","journal-title":"Rhinology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.1093\/jac\/dkq212","article-title":"Standardized comparison of antiseptic efficacy of triclosan, PVP-iodine, octenidine dihydrochloride, polyhexanide and chlorhexidine digluconate","volume":"65","author":"Koburger","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Liang, C., Jiang, Q., Yu, Y., Xu, T., Sun, H., Deng, F., and Yu, X. (2021). Antibacterial evaluation of lithium-loaded nanofibrous poly (L-lactic acid) membranes fabricated via an electrospinning strategy. Front. Bioeng. Biotechnol., 9.","DOI":"10.3389\/fbioe.2021.676874"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1093\/icvts\/ivy248","article-title":"Povidone-iodine results in rapid killing of thymic epithelial tumour cells through cellular fixation","volume":"28","author":"Lee","year":"2019","journal-title":"Interact. Cardiovasc. Thorac. Surg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e202201875","DOI":"10.26508\/lsa.202201875","article-title":"Cell death and barrier disruption by clinically used iodine concentrations","volume":"6","author":"Steins","year":"2023","journal-title":"Life Sci. Alliance"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/24\/4822\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T14:05:26Z","timestamp":1766498726000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/30\/24\/4822"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,18]]},"references-count":32,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["molecules30244822"],"URL":"https:\/\/doi.org\/10.3390\/molecules30244822","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,18]]}}}