{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T14:24:20Z","timestamp":1785507860978,"version":"3.56.0"},"reference-count":76,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hebei Province key research and development program","award":["22372503D"],"award-info":[{"award-number":["22372503D"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Herba Hyssopi is a key remedy in Uighur medicine for asthma and cough, frequently used as the monarch or minister herb in prescriptions. However, the lack of effective quality assessment methods complicates the detection of adulteration with common substitutes. In this study, UPLC-LTQ-Orbitrap-MS, network pharmacology, molecular docking, and cell experiments were employed to establish scientific and effective quality control methods to differentiate Hyssopus cuspidatus Boiss from its common adulterants. The results showed that a total of 41 chemical constituents were identified from Herba Hyssopi. Network pharmacology analysis revealed 133 potential target genes associated with its therapeutic actions, among which EGFR, MMP9, TNF, PTGS2, MAPK3, ESR1, and TP53 emerged as key targets. Cellular experiments further demonstrated that diosmin, linarin, and rosmarinic acid significantly suppressed nitric oxide (NO) generation and the release of pro-inflammatory cytokines. Based on these findings, a validated HPLC method was established for the simultaneous quantification of these three bioactive markers, providing a reliable tool for the quality assessment and authentication of Herba Hyssopi. This study offers a scientific basis for improving the standardization and quality control of Herba Hyssopi in traditional medicine applications.<\/jats:p>","DOI":"10.3390\/molecules31020205","type":"journal-article","created":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T15:09:36Z","timestamp":1767712176000},"page":"205","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Study on Comprehensive Quality Control of Herba Hyssopi Based on Chemical Components and Pharmacological Mechanism Action"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6319-4569","authenticated-orcid":false,"given":"Zhenxia","family":"Zhao","sequence":"first","affiliation":[{"name":"Hebei Institute of Drug and Medical Device Inspection, Shijiazhuang 050027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7582-8121","authenticated-orcid":false,"given":"Jiangning","family":"Peng","sequence":"additional","affiliation":[{"name":"Hebei Institute of Drug and Medical Device Inspection, Shijiazhuang 050027, China"},{"name":"College of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1204-3814","authenticated-orcid":false,"given":"Yingfeng","family":"Du","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Yang","sequence":"additional","affiliation":[{"name":"Hebei Institute of Drug and Medical Device Inspection, Shijiazhuang 050027, China"},{"name":"School of Pharmacy, Henan University, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilan","family":"Fan","sequence":"additional","affiliation":[{"name":"Hebei Institute of Drug and Medical Device Inspection, Shijiazhuang 050027, China"},{"name":"School of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Li","sequence":"additional","affiliation":[{"name":"Hebei Institute of Drug and Medical Device Inspection, Shijiazhuang 050027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amatjan","family":"Ayupbek","sequence":"additional","affiliation":[{"name":"NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Uyghur Medicine, XinJiang Uygur Autonomous Region Institute for Drug Control, Urumqi 830054, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Hebei Institute of Drug and Medical Device Inspection, Shijiazhuang 050027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4328-4026","authenticated-orcid":false,"given":"Yongli","family":"Liu","sequence":"additional","affiliation":[{"name":"Hebei Institute of Drug and Medical Device Inspection, Shijiazhuang 050027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1111\/j.1365-2222.2011.03917.x","article-title":"Lung damage and airway remodelling in severe asthma","volume":"42","author":"Brightling","year":"2012","journal-title":"Clin. 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