{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:29:11Z","timestamp":1768508951233,"version":"3.49.0"},"reference-count":38,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T00:00:00Z","timestamp":1768089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Deanship at the University of Ha\u2019il\u2014Saudi Arabia","award":["RG-25-036"],"award-info":[{"award-number":["RG-25-036"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Erica carnea L. has recently gained attention as a promising natural source of antioxidants suitable for food and beverage applications. This study aimed to obtain an antioxidant-enriched extract by optimizing the key extraction variables. A full factorial design was used to evaluate the effects of ethanol concentration, extraction time, and temperature, followed by validation through multiple antioxidant assays, including DPPH, ABTS, hydroxyl radical scavenging, lipid peroxidation inhibition, and metal chelation. The most efficient extraction was achieved at 30% ethanol, 120 min, and 50 \u00b0C, yielding IC50 values of 18.42 \u03bcg\/mL (LP), 15.04 \u03bcg\/mL (DPPH), 5.14 \u03bcg\/mL (MC), 11.28 \u03bcg\/mL (OH), and 10.06 \u03bcg\/mL (ABTS), in agreement with the model predictions. Extraction kinetics were described using an unsteady-state diffusion model, supported by low root mean square (RMS) values and high coefficients of determination. Thermodynamic analysis indicated an irreversible, endothermic process, highlighting the energetic requirements for phenolic release from plant tissue. The combined statistical and kinetic approach provides a clearer understanding of how process variables influence antioxidant recovery. In addition to strong antioxidant activity, the optimized extract showed measurable cytotoxic and antibacterial effects. Overall, these findings highlight E. carnea as a valuable material for developing antioxidant-rich formulations, with extraction efficiency governed by predictable kinetic and thermodynamic behavior.<\/jats:p>","DOI":"10.3390\/molecules31020245","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T07:30:34Z","timestamp":1768203034000},"page":"245","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Designing Antioxidant-Enriched Extracts from Erica carnea L.: Optimization, Kinetics, and Thermodynamic Insights"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2180-8303","authenticated-orcid":false,"given":"Violeta","family":"Jevtovic","sequence":"first","affiliation":[{"name":"Chemistry Department, College of Science, University of Ha\u2019il, Ha\u2019il 81451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2971-7593","authenticated-orcid":false,"given":"Khulood Fahad Saud","family":"Alabbosh","sequence":"additional","affiliation":[{"name":"Biology Department, College of Science, University of Ha\u2019il, Ha\u2019il 81451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buthainah Ameen Al","family":"Shankiti","sequence":"additional","affiliation":[{"name":"Chemistry Department, College of Science, University of Ha\u2019il, Ha\u2019il 81451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarfah Abdulrahman M.","family":"Alaskar","sequence":"additional","affiliation":[{"name":"Chemistry Department, College of Science, University of Ha\u2019il, Ha\u2019il 81451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reem Ali","family":"Alyami","sequence":"additional","affiliation":[{"name":"Chemistry Department, College of Science, University of Ha\u2019il, Ha\u2019il 81451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walaa I.","family":"El-Sofany","sequence":"additional","affiliation":[{"name":"Chemistry Department, College of Science, University of Ha\u2019il, Ha\u2019il 81451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7885-0476","authenticated-orcid":false,"given":"Vesna Stankov","family":"Jovanovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Mathematics, University of Ni\u0161, 18000 Ni\u0161, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9351-331X","authenticated-orcid":false,"given":"Jelena","family":"Nikoli\u0107","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Mathematics, University of Ni\u0161, 18000 Ni\u0161, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vesna","family":"Veli\u010dkovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Physics and Materials, Faculty of Technical Sciences, University of Kragujevac, 32102 \u010ca\u010dak, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1176-7103","authenticated-orcid":false,"given":"Odeh A. 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Antioxid."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100257","DOI":"10.1016\/j.focha.2023.100257","article-title":"Collating Antioxidant, Reducing and Metal Chellating Properties of Spices and Acacia","volume":"2","author":"Yadav","year":"2023","journal-title":"Food Chem. Adv."},{"key":"ref_3","first-page":"598","article-title":"Free Radicals, Antioxidants, and Human Disease: Where Are We Now?","volume":"119","author":"Halliwell","year":"1992","journal-title":"J. Lab. Clin. Med."},{"key":"ref_4","first-page":"750","article-title":"In Vitro Antioxidant and Free Radical Scavenging Activity of Curcuma longa, Acorus calamus and Camellia sinensis","volume":"13","author":"Rahul","year":"2022","journal-title":"Food Nutr. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.sajb.2018.06.012","article-title":"Biological Activity of Extracts of Traditional Wild Medicinal Plants from the Balkan Peninsula","volume":"120","author":"Zengin","year":"2019","journal-title":"S. Afr. J. Bot."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.indcrop.2017.11.015","article-title":"Subcritical Water Extraction as an Environmentally Friendly Technique to Recover Bioactive Compounds from Traditional Serbian Medicinal Plants","volume":"111","author":"Barroso","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.indcrop.2017.06.023","article-title":"Phenolic Bioactives and Associated Antioxidant and Anti-Hyperglycemic Functions of Select Species of Apiaceae Family Targeting for Type 2 Diabetes Relevant Nutraceuticals","volume":"107","author":"Saleem","year":"2017","journal-title":"Ind. Crops Prod."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"767","DOI":"10.3390\/molecules19010767","article-title":"Acetylcholinesterase Inhibitory, Antioxidant and Phytochemical Properties of Selected Medicinal Plants of the Lamiaceae Family","volume":"19","author":"Kindl","year":"2014","journal-title":"Molecules"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.supflu.2017.03.023","article-title":"Application of Conventional and Non-Conventional Extraction Approaches for Extraction of Erica carnea L.: Chemical Profile and Biological Activity","volume":"128","year":"2017","journal-title":"J. Supercrit. Fluids"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Jevtovi\u0107, V., Alabbosh, K.F.S., Alyami, R.A., Alshammari, M.R., Alshammari, B., Ma\u0161kovi\u0107, P., Ma\u0161kovi\u0107, J., Nikoli\u0107, J., and Miti\u0107, M. (2025). Unveiling Cytotoxic Bioactive Compounds from Erica carnea L.: Extraction Optimization, Kinetic Modeling, and Biological Activity Assessment. Plants, 14.","DOI":"10.3390\/plants14111679"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Munteanu, I.G., and Apetrei, C. (2021). Analytical Methods Used in Determining Antioxidant Activity: A Review. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22073380"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Carciochi, R.A., Sologubik, C.A., Fern\u00e1ndez, M.B., Manrique, G.D., and Galv\u00e1n D\u2019Alessandro, L. (2018). Extraction of Antioxidant Phenolic Compounds from Brewer\u2019s Spent Grain: Optimization and Kinetics Modeling. Antioxidants, 7.","DOI":"10.3390\/antiox7040045"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.foodchem.2004.08.050","article-title":"Optimization of Extraction of Phenolic Compounds from Wheat Using Response Surface Methodology","volume":"93","author":"Shahidi","year":"2005","journal-title":"Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"100906","DOI":"10.1016\/j.cscee.2024.100906","article-title":"Optimized Extraction of Phenolic Compounds and Antioxidant Activity from Cannabis Co-Products via Solvent\u2013Ultrasound Extraction and Response Surface Methodology","volume":"10","author":"Soultana","year":"2024","journal-title":"Case Stud. Chem. Environ. Eng."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Kotsou, K., Papagiannoula, A., Chatzimitakos, T., Athanasiadis, V., Bozinou, E., Sfougaris, A.I., and Lalas, S.I. (2024). Optimization of Extraction Parameters to Enhance the Antioxidant Properties of Pyrus spinosa Fruit Extract. Beverages, 10.","DOI":"10.3390\/beverages10030056"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.indcrop.2016.07.015","article-title":"Kinetics and Thermodynamics of Solid\u2013Liquid Extraction of Total Polyphenols, Antioxidants and Extraction Yield from Asteraceae Plants","volume":"91","author":"Valinger","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Jevtovi\u0107, V., Alabbosh, K.F.S., Alyami, R.A., Alreshidi, M.A., Alshammari, M.R., Alshammari, B., Miti\u0107, J., and Miti\u0107, M. (2025). Optimization and Kinetic Modelling of Hydroxycinnamic Acid Extraction from Anethum graveolens Leaves. Processes, 13.","DOI":"10.3390\/pr13051297"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Kumar, S., Mishra, A., and Pandey, A.K. (2013). Antioxidant-Mediated Protective Effect of Parthenium hysterophorus against Oxidative Damage Using In Vitro Models. BMC Complement Altern. Med., 13.","DOI":"10.1186\/1472-6882-13-120"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.jff.2017.01.011","article-title":"Antioxidant Activities and Phenolic Profiles in 14 Subtropical Fruit Leaves","volume":"30","author":"Chen","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.indcrop.2017.03.012","article-title":"Antioxidant and Antihyperglycemic Activity of Phenolics from Sugarcane Bagasse","volume":"101","author":"Zheng","year":"2017","journal-title":"Ind. Crops Prod."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/S0140-6736(94)92211-X","article-title":"Free Radicals, Antioxidants, and Human Disease: Curiosity, Cause, or Consequence?","volume":"344","author":"Halliwell","year":"1994","journal-title":"Lancet"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/0014-5793(96)00323-7","article-title":"Antioxidant Activities of Carotenes and Xanthophylls","volume":"384","author":"Miller","year":"1996","journal-title":"FEBS Lett."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Chadni, M., Isidore, E., Diemer, E., Ouguir, O., Brunois, F., Catteau, R., Cassan, L., and Ioannou, I. (2022). Optimization of Extraction Conditions to Improve Chlorogenic Acid Content and Antioxidant Activity of Witloof Chicory Root Extracts. Foods, 11.","DOI":"10.3390\/foods11091217"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"100664","DOI":"10.1016\/j.fbio.2020.100664","article-title":"Phytochemical Profile and Antioxidant Properties of Tomato By-Products as Affected by Extraction Solvents and Their Potential Application in Olive Oil","volume":"36","author":"Azabou","year":"2020","journal-title":"Food Biosci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Jevtovic, V., Alabbosh, K.F.S., Pr\u017ei\u2019c, Z., Nikoli\u2019c, J., Ali Alyami, R., Alshammari, M.R., Alshammari, B., Rakic, V., Alshammari, O.A.O., and Miti\u2019c, M. (2025). Targeted Recovery of Phenolic Antioxidants from Grape Stems: A Sequential Approach. Molecules, 30.","DOI":"10.3390\/molecules30173546"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e32421","DOI":"10.1016\/j.heliyon.2024.e32421","article-title":"Kinetics and Thermodynamic Studies on Oil Extraction from Ghanaian Cashew Kernel Using Hexane","volume":"10","author":"Baidoo","year":"2024","journal-title":"Heliyon"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1080\/10942912.2016.1163266","article-title":"Aqueous extraction kinetics of phenolic compounds from jamun (Syzygium cumini L.) seeds","volume":"20","author":"Balyan","year":"2017","journal-title":"Int. J. Food Prop."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.seppur.2012.09.004","article-title":"Kinetic modeling of total polyphenol extraction from grape marc and characterization of the extracts","volume":"100","author":"Brandelli","year":"2012","journal-title":"Sep. Purif. Technol."},{"key":"ref_29","first-page":"1984","article-title":"Kinetic and Thermodynamic Parameters of Biodiesel Oxidation with Synthetic Antioxidants: Simplex Centroid Mixture Design","volume":"25","author":"Borsato","year":"2014","journal-title":"J. Braz. Chem. Soc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.foodchem.2007.11.028","article-title":"Improving the antioxidant activity of buckwheat (Fagopyrum tataricm Gaertn) sprout with trace elementwater","volume":"108","author":"Hsu","year":"2008","journal-title":"Food Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.foodchem.2005.03.028","article-title":"Antioxidant Activities of Extracts from Selected Culinary Herbs and Spices","volume":"97","author":"Hinneburg","year":"2006","journal-title":"Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1021\/jf9908188","article-title":"Characterization of the Total Free Radical-Scavenger Capacity of Vegetable Oils and Oil Fractions Using 2,2-Diphenyl-1-picrylhydrazyl Radical","volume":"48","author":"Wichers","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"7832","DOI":"10.1021\/jf0512353","article-title":"Assessing the Antioxidant Activity of Melanoidins from Coffee Brews by Different Antioxidant Methods","volume":"53","author":"Morales","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1006\/abbi.1994.1485","article-title":"Action of Phenolic Derivatives (Acetaminophen, Salicylate, and 5-Aminosalicylate) as Inhibitors of Membrane Lipid Peroxidation and as Peroxyl Radical Scavengers","volume":"315","author":"Dinis","year":"1994","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_35","first-page":"40","article-title":"Biological Activities and Chemical Composition of Morus Leaves Extracts Obtained by Maceration and Supercritical Fluid Extraction","volume":"117","year":"2016","journal-title":"J. Supercrit. Fluids"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.indcrop.2014.09.044","article-title":"Antioxidant and Biological Activity of Chamomile Extracts Obtained by Different Techniques: The Perspective of Using Superheated Water for Isolation of Biologically Active Compounds","volume":"65","year":"2015","journal-title":"Ind. Crops Prod."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.fct.2018.09.045","article-title":"Comparative In Vitro Studies of the Biological Potential and Chemical Composition of Stems, Leaves and Berries of Aronia melanocarpa Extracts Obtained by Subcritical Water Extraction","volume":"121","author":"Zengin","year":"2018","journal-title":"Food Chem. Toxicol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1080\/01496395.2014.978019","article-title":"Kinetics of the Solid\u2013Liquid Extraction of Phenolic Antioxidants and Antioxidant Capacity from Hop (Humulus lupulus L.)","volume":"50","year":"2015","journal-title":"Sep. Sci. Technol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/2\/245\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T05:32:34Z","timestamp":1768455154000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/2\/245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,11]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["molecules31020245"],"URL":"https:\/\/doi.org\/10.3390\/molecules31020245","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,11]]}}}