{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T21:21:38Z","timestamp":1774128098996,"version":"3.50.1"},"reference-count":130,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Alicyclobacillus acidoterrestris is an acidothermophilic bacterium considered a significant challenge to the food industry, particularly in the production of fruit juices and concentrates. Its ability to survive pasteurization and form spores and biofilms makes it a persistent contaminant that can spoil products and generate off-flavors even during product storage. Recent studies have increasingly focused on developing new strategies to eliminate both vegetative cells and biofilms, with special attention on natural compounds such as plant extracts, essential oils and antimicrobial metabolites. These natural agents offer promising alternatives for controlling A. acidoterrestris and might contribute to improvement in safety and quality of juice products. This article presents a comprehensive overview of current strategies for controlling Alicyclobacillus species in food processing environments, with an emphasis on A. acidoterrestris as a major spoilage organism in the fruit juice industry. It summarizes the established physical and chemical control methods, as well as highlights emerging novel approaches involving natural-origin antimicrobial compounds considered useful for mitigating Alicyclobacillus contamination.<\/jats:p>","DOI":"10.3390\/molecules31020257","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T14:57:25Z","timestamp":1768229845000},"page":"257","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Alicyclobacillus acidoterrestris Eradication Strategies with Physical Methods and Natural-Origin Substances Intended for Food Industry"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9011-2548","authenticated-orcid":false,"given":"Agnieszka","family":"Tyfa","sequence":"first","affiliation":[{"name":"Institute of Agricultural and Food Biotechnology\u2014State Research Institute (IBPRS-PIB), Food Quality Department, Al. Marsza\u0142ka J. Pi\u0142sudskiego 84, 92-202 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4611-9109","authenticated-orcid":false,"given":"Alina","family":"Kunicka-Styczy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Sugar Industry and Food Safety Management, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, W\u00f3lcza\u0144ska 171\/173, 90-530 Lodz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6422","DOI":"10.1007\/s11947-025-03836-3","article-title":"Exploitation of Impedometric Analysis and Fluorescence Microscopy: Assessment of Alicyclobacillus acidoterrestris Life Cycle and Innovative Inactivation Strategy","volume":"18","author":"Bancalari","year":"2025","journal-title":"Food Bioprocess Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"625","DOI":"10.3390\/microorganisms3040625","article-title":"Alicyclobacillus spp.: New Insights on Ecology and Preserving Food Quality through New Approaches","volume":"3","author":"Ciuffreda","year":"2015","journal-title":"Microorganisms"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"18","DOI":"10.15586\/ijfs.v37i3.3128","article-title":"Recent Advances in Innovative Strategies for Controlling Microbial Growth in Food System: A Concise Review","volume":"37","author":"Pellegrino","year":"2025","journal-title":"Ital. J. Food Sci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Tyfa, A., Kunicka-Styczy\u0144ska, A., Molska, M., Gruska, R.M., and Baryga, A. (2025). Clove, Cinnamon, and Peppermint Essential Oils as Antibiofilm Agents Against Alicyclobacillus acidoterrestris. Molecules, 30.","DOI":"10.3390\/molecules30112312"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Shymialevich, D., W\u00f3jcicki, M., and Soko\u0142owska, B. (2024). The Novel Concept of Synergically Combining: High Hydrostatic Pressure and Lytic Bacteriophages to Eliminate Vegetative and Spore-Forming Bacteria in Food Products. Foods, 13.","DOI":"10.3390\/foods13162519"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.foodcont.2009.05.002","article-title":"Combining Eugenol and Cinnamaldehyde to Control the Growth of Alicyclobacillus acidoterrestris","volume":"21","author":"Bevilacqua","year":"2010","journal-title":"Food Control."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.foodcont.2012.12.001","article-title":"Biofilm Formation in Food Industries: A Food Safety Concern","volume":"31","author":"Srey","year":"2013","journal-title":"Food Control."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Carrascosa, C., Raheem, D., Ramos, F., Saraiva, A., and Raposo, A. (2021). Microbial Biofilms in the Food Industry\u2014A Comprehensive Review. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18042014"},{"key":"ref_9","first-page":"224","article-title":"Fruchtsaftverderb Durch Bacillen: Isolierung Und Charakterisierung Des Verderbserregers","volume":"179","author":"Cerny","year":"1984","journal-title":"Eur. Food Res. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1271\/bbb.60.543","article-title":"Isolation and Identification of Alicyclobacillus acidoterrestris from Acidic Beverages","volume":"60","author":"Yamazaki","year":"1996","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_11","first-page":"292","article-title":"Spoilage of Iced Tea by Alicyclobacillus","volume":"52","author":"Duong","year":"2000","journal-title":"Food Aust."},{"key":"ref_12","unstructured":"Bah\u00e7eci, K.S., G\u00f6kmen, V., and Acar, J. (2003). Occurrence of Alicyclobacillus acidoterrestris on Apples and in Apple Juice Concentrates and Effect of Process Technology on A. acidoterrestris Spores in Apple Juice. Fruit Process., 10 328\u2013331."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1111\/j.1472-765X.2007.02229.x","article-title":"Membrane Filtration Method for Enumeration and Isolation of Alicyclobacillus spp. from Apple Juice","volume":"45","author":"Lee","year":"2007","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1016\/j.fm.2010.06.010","article-title":"Alicyclobacillus acidoterrestris in Pasteurized Exotic Brazilian Fruit Juices: Isolation, Genotypic Characterization and Heat Resistance","volume":"27","author":"McKnight","year":"2010","journal-title":"Food Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"M130","DOI":"10.1111\/j.1750-3841.2010.02032.x","article-title":"Characterization of a Wild Strain of Alicyclobacillus acidoterrestris: Heat Resistance and Implications for Tomato Juice","volume":"76","author":"Bevilacqua","year":"2011","journal-title":"J. Food Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.fm.2010.10.008","article-title":"Prevalence, Concentration, Spoilage, and Mitigation of Alicyclobacillus spp. in Tropical and Subtropical Fruit Juice Concentrates","volume":"28","author":"Danyluk","year":"2011","journal-title":"Food Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Zhang, J., Yue, T., and Yuan, Y. (2013). Alicyclobacillus Contamination in the Production Line of Kiwi Products in China. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0067704"},{"key":"ref_18","first-page":"73","article-title":"Isolation of Alicyclobacillus acidoterrestris from Commercial Spoiled Apple Juice and Study on Some Influence Parameters on Its Growth in Apple Juice","volume":"5","author":"Tayefe","year":"2014","journal-title":"Egypt. Acad. J. Biol. Sci. H. Bot."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s40550-015-0008-0","article-title":"Fruit Juice and Puree Characteristics Influence Enrichment Requirements for Real-Time PCR Detection of Alicyclobacillus acidoterrestris","volume":"2","author":"Shayanfar","year":"2015","journal-title":"Int. J. Food Contam."},{"key":"ref_20","first-page":"5","article-title":"Incidence of Alicyclobacillus spp. in Polish Apple and Dark Berry Juice Concentrates and the Ability of Isolated A. acidoterrestris Strains to Spoilage of These Juices","volume":"71","author":"Niezgoda","year":"2016","journal-title":"PNiTPRS"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1590\/S1517-83822013000400015","article-title":"Chemicals and Lemon Essential Oil Effect on Alicyclobacillus acidoterrestris Viability","volume":"44","author":"Maldonado","year":"2013","journal-title":"Braz. J. Microbiol."},{"key":"ref_22","first-page":"29","article-title":"Bior\u00f3\u017cnorodno\u015b\u0107 Szczep\u00f3w Alicyclobacillus acidoterrestris","volume":"65","author":"Niezgoda","year":"2010","journal-title":"Pr. Inst. I Lab. Badaw. Przemys\u0142u Spo\u017cywczego"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.ijfoodmicro.2010.07.003","article-title":"Identification and Haplotype Distribution of Alicyclobacillus spp. from Different Juices and Beverages","volume":"142","author":"Durak","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1111\/j.1365-2621.2006.01427.x","article-title":"Alicyclobacillus acidoterrestris: An Increasing Threat to the Fruit Juice Industry?","volume":"43","author":"Walker","year":"2008","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_25","first-page":"87","article-title":"The Possibility of Thermal Inactivation of Alicyclobacillus acidoterrestris Spores in Fruit and Vegetable Juices","volume":"75","author":"Papiewska","year":"2011","journal-title":"Biotechnol. Food Sci."},{"key":"ref_26","first-page":"386","article-title":"Inactivation of Alicyclobacillus acidoterrestris by Non Thermal Processing Technologies\u2014A Review","volume":"1","author":"Kumar","year":"2013","journal-title":"Int. J. Adv. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ijfoodmicro.2011.02.002","article-title":"Isolation of Alicyclobacillus and the Influence of Different Growth Parameters","volume":"146","author":"Witthuhn","year":"2011","journal-title":"Int. J. Food Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1111\/j.1472-765X.2004.01636.x","article-title":"Identification of Medicinal Off-Flavours Generated by Alicyclobacillus Species in Orange Juice Using GC-Olfactometry and GC-MS","volume":"40","author":"Gocmen","year":"2005","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Goto, K. (2007). Differentiation and Identification of Alicyclobacillus Species. Alicyclobacillus Thermophilic Acidophilic Bacilli, Springer.","DOI":"10.1007\/978-4-431-69850-0_5"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.1472-765X.2007.02307.x","article-title":"Identification of Thermo-Acidophilic Bacteria Isolated from the Soil of Several Japanese Fruit Orchards","volume":"46","author":"Goto","year":"2008","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1080\/10408410490435089","article-title":"Alicyclobacillus spp. in the Fruit Juice Industry: History, Characteristics, and Current Isolation\/Detection Procedures","volume":"30","author":"Chang","year":"2004","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/j.1365-2621.2005.tb07201.x","article-title":"Best Estimated Aroma and Taste Detection Threshold for Guaiacol in Water and Apple Juice","volume":"70","author":"Eisele","year":"2005","journal-title":"J. Food Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5984","DOI":"10.1021\/jf060602n","article-title":"Odor Thresholds of Microbially Induced Off-Flavor Compounds in Apple Juice","volume":"54","author":"Siegmund","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1046\/j.1472-765X.1997.00373.x","article-title":"Methods for the Detection and Enumeration of Alicyclobacillus acidoterrestris and Investigation of Growth and Production of Taint in Fruit Juice and Fruit Juice-Containing Drinks","volume":"24","author":"Pettipher","year":"1997","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.4315\/0362-028X-63.11.1517","article-title":"Detection of Guaiacol Produced by Alicyclobacillus acidoterrestris in Apple Juice by Sensory and Chromatographic Analyses, and Comparison with Spore and Vegetative Cell Populations","volume":"63","author":"Orr","year":"2000","journal-title":"J. Food Prot."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1046\/j.1365-2621.1999.00243.x","article-title":"Alicyclobacillus acidoterrestris in Fruit Juices and Its Control by Nisin","volume":"34","author":"Komitopoulou","year":"1999","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/0043-1354(95)00173-5","article-title":"Taste and Odour Threshold Concentrations of Potential Potable Water Contaminants","volume":"30","author":"Young","year":"1996","journal-title":"Water Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1046\/j.1472-765X.2003.01251.x","article-title":"Role of Alicyclobacillus acidoterrestris in the Development of a Disinfectant Taint in Shelf-Stable Fruit Juice","volume":"36","author":"Jensen","year":"2003","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/lam.13385","article-title":"Control of the Growth of Alicyclobacillus acidoterrestris in Industrialized Orange Juice Using Rosemary Essential Oil and Nisin","volume":"72","author":"Silva","year":"2021","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.foodcont.2013.06.051","article-title":"Combined Effect of Natural Antimicrobials and Thermal Treatments on Alicyclobacillus acidoterrestris Spores","volume":"35","author":"Huertas","year":"2014","journal-title":"Food Control."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Fan, Q., Liu, C., Gao, Z., Hu, Z., Wang, Z., Xiao, J., Yuan, Y., and Yue, T. (2021). Inactivation Effect of Thymoquinone on Alicyclobacillus acidoterrestris Vegetative Cells, Spores, and Biofilms. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.679808"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4158","DOI":"10.1128\/AEM.68.8.4158-4161.2002","article-title":"Inhibitory Effects of High Pressure and Heat on Alicyclobacillus acidoterrestris Spores in Apple Juice","volume":"68","author":"Lee","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1023\/A:1025167818169","article-title":"Inactivation of Alicyclobacillus acidoterrestris Vegetative Cells in Model System, Apple, Orange and Tomato Juices by High Hydrostatic Pressure","volume":"19","author":"Alpas","year":"2003","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.ijfoodmicro.2011.02.019","article-title":"Germination and Inactivation of Bacillus Coagulans and Alicyclobacillus acidoterrestris Spores by High Hydrostatic Pressure Treatment in Buffer and Tomato Sauce","volume":"152","author":"Vercammen","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref_45","first-page":"187","article-title":"Zastosowanie Pasteryzacji Wysokoci\u015bnieniowej Do Inaktywacji Przetrwalnik\u00f3w Alicyclobacillus acidoterrestris w Soku Jab\u0142kowym","volume":"3","author":"Niezgoda","year":"2012","journal-title":"\u017bywno\u015b\u0107 Nauka Technol. Jako\u015b\u0107"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1111\/j.1472-765X.2007.02219.x","article-title":"Effects of High-Pressure Homogenization on the Survival of Alicyclobacillus acidoterrestris in a Laboratory Medium","volume":"45","author":"Bevilacqua","year":"2007","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2538","DOI":"10.4315\/0362-028X-67.11.2538","article-title":"Radiation-Heat Synergism for Inactivation of Alicyclobacillus acidoterrestris Spores in Citrus Juice","volume":"67","author":"Nakauma","year":"2004","journal-title":"J. Food Prot."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"339","DOI":"10.4315\/0362-028X.JFP-13-133","article-title":"Inactivation of Alicyclobacillus acidoterrestris Spores in Apple and Orange Juice Concentrates by Gamma Irradiation","volume":"77","author":"Lee","year":"2014","journal-title":"J. Food Prot."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.ijfoodmicro.2013.08.015","article-title":"UV-C Light Inactivation and Modeling Kinetics of Alicyclobacillus acidoterrestris Spores in White Grape and Apple Juices","volume":"166","author":"Baysal","year":"2013","journal-title":"Int. J. Food Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"102299","DOI":"10.1016\/j.ifset.2020.102299","article-title":"Effect of Single and Combined UV-C and Ultra-High Pressure Homogenisation Treatments on Inactivation of Alicyclobacillus acidoterrestris Spores in Apple Juice","volume":"60","author":"Gervilla","year":"2020","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Martin, S., and Feng, H. (2009). Novel Methods for Biofilm Control and Removal from Food Processing Equipment. Biofilms in the Food and Beverage Industries, Elsevier.","DOI":"10.1533\/9781845697167.3.359"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1111\/j.1745-4549.2009.00407.x","article-title":"Effect of ultrasonic treatments on thermoacidophilic Alicyclobacillus acidoterrestris in apple juice","volume":"33","author":"Yuan","year":"2009","journal-title":"J. Food Process. Preserv."},{"key":"ref_53","first-page":"46","article-title":"New Methods for Inactivation of Alicyclobacillus acidoterrestris Spores in Apple Juice Concentrate","volume":"2","author":"Djas","year":"2011","journal-title":"Chall. Mod. Technol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1007\/s12393-011-9045-5","article-title":"Processing of Fruits and Fruit Juices by Novel Electrotechnologies","volume":"4","author":"Evrendilek","year":"2012","journal-title":"Food. Eng. Rev."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.foodres.2013.02.033","article-title":"Microwave Food Processing\u2014A Review","volume":"52","author":"Chandrasekaran","year":"2013","journal-title":"Food Res. Int."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.ifset.2009.09.003","article-title":"Use of Microwave Processing to Reduce the Initial Contamination by Alicyclobacillus acidoterrestris in a Cream of Asparagus and Effect of the Treatment on the Lipid Fraction","volume":"11","author":"Giuliani","year":"2010","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_57","first-page":"705","article-title":"Efficacy of Microwave against Alicyclobacillus acidoterrestris in Apple Juice","volume":"2","author":"Zhang","year":"2014","journal-title":"Sch. J. Eng. Tech."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.4315\/0362-028X-67.6.1190","article-title":"Controlled Release of Antimicrobial Compounds from Highly Swellable Polymers","volume":"67","author":"Buonocore","year":"2004","journal-title":"J. Food Prot."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"E379","DOI":"10.1111\/j.1365-2621.2004.tb09899.x","article-title":"Effect of Ag-containing Nano-composite Active Packaging System on Survival of Alicyclobacillus acidoterrestris","volume":"69","author":"Nobile","year":"2004","journal-title":"J. Food Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.ijfoodmicro.2003.09.003","article-title":"The Use of Chlorine Dioxide to Control Alicyclobacillus acidoterrestris Spores in Aqueous Suspension and on Apples","volume":"92","author":"Lee","year":"2004","journal-title":"Int. J. Food Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.ijfoodmicro.2005.11.023","article-title":"Efficacy of Chlorine Dioxide Gas against Alicyclobacillus acidoterrestris Spores on Apple Surfaces","volume":"108","author":"Lee","year":"2006","journal-title":"Int. J. Food Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.ijfoodmicro.2009.09.015","article-title":"Inactivation of Alicyclobacillus acidoterrestris Spores in Apple Juice by Supercritical Carbon Dioxide","volume":"136","author":"Bae","year":"2009","journal-title":"Int. J. Food Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2013.11.035","article-title":"Efficacy of Ozone against Alicyclobacillus acidoterrestris Spores in Apple Juice","volume":"172","author":"Torlak","year":"2014","journal-title":"Int. J. Food Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.5604\/17322693.1133100","article-title":"Lizozym\u2014Wyst\u0119powanie w Przyrodzie, W\u0142a\u015bciwo\u015bci Biologiczne i Mo\u017cliwo\u015bci Zastosowa\u0144","volume":"68","author":"Gajda","year":"2014","journal-title":"Post\u0119py Hig. I Med. Do\u015bwiadczalnej"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1111\/ijfs.12302","article-title":"Effects of Lysozyme on Alicyclobacillus acidoterrestris under Laboratory Conditions","volume":"49","author":"Bevilacqua","year":"2014","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_66","first-page":"47","article-title":"Antimicrobial Effectiveness of Lysozyme against Alicyclobacillus acidoterrestris","volume":"29","author":"Bevilacqua","year":"2007","journal-title":"Adv. Food Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"861","DOI":"10.4315\/0362-028X-69.4.861","article-title":"Antimicrobial Effectiveness of Lysozyme Immobilized on Polyvinylalcohol-Based Film against Alicyclobacillus acidoterrestris","volume":"69","author":"Conte","year":"2006","journal-title":"J. Food Prot."},{"key":"ref_68","first-page":"44","article-title":"Wp\u0142yw Nizyny i Lizozymu Na Wzrost Szczep\u00f3w Alicyclobacillus acidoterrestris Oraz Mo\u017cliwo\u015b\u0107 Zastosowania Tych Zwi\u0105zk\u00f3w Jako Biokonserwant\u00f3w w Soku Jab\u0142kowym","volume":"4","author":"Niezgoda","year":"2012","journal-title":"\u017bYWNO\u015a\u0106 Nauka. Technol. Jako\u015b\u0107"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Chandrasekaran, M., Kim, K., and Chun, S. (2020). Antibacterial Activity of Chitosan Nanoparticles: A Review. Processes, 8.","DOI":"10.3390\/pr8091173"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1590\/S0104-14282009000300013","article-title":"A Review of the Antimicrobial Activity of Chitosan","volume":"19","author":"Goy","year":"2009","journal-title":"Pol\u00edmeros"},{"key":"ref_71","first-page":"142","article-title":"Effectiveness of Pasteurization on Alicyclobacillus acidoterrestris Spores in the Presence of Low Molecular Weight Chitosan","volume":"15","author":"Falcone","year":"2003","journal-title":"J. Food Sci."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"130214","DOI":"10.1016\/j.ijbiomac.2024.130214","article-title":"Antibacterial Effects of Various Molecular Weight Chitosans against Alicyclobacillus acidoterrestris in Orange Juice","volume":"262","author":"Ulfadillah","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"5408","DOI":"10.1007\/s13197-014-1666-2","article-title":"Strategies for the Use of Bacteriocins in Gram-Negative Bacteria: Relevance in Food Microbiology","volume":"52","author":"Vanetti","year":"2015","journal-title":"J. Food Sci. Technol."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Naskar, A., and Kim, K.-S. (2021). Potential Novel Food-Related and Biomedical Applications of Nanomaterials Combined with Bacteriocins. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13010086"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"107638","DOI":"10.1016\/j.fbio.2025.107638","article-title":"A Nisin-Mediated Organic-Inorganic Hybrid for the Inhibition of Alicyclobacillus acidoterrestris in Apple Juice","volume":"73","author":"Jia","year":"2025","journal-title":"Food Biosci."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1006\/fmic.1999.0309","article-title":"Use of Nisin for Inhibition of Alicyclobacillus acidoterrestris in Acidic Drinks","volume":"17","author":"Yamazaki","year":"2000","journal-title":"Food Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1590\/S1517-83822009000300024","article-title":"Microbial Modelling of Thermal Resistance of Alicyclobacillus acidoterrestris CRA 7152 Spores in Concentrated Orange Juice with Nisin Addition","volume":"40","author":"Massague","year":"2009","journal-title":"Braz. J. Microbiol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1007\/s11947-016-1822-y","article-title":"The Effect of RF Treatment Combined with Nisin Against Alicyclobacillus Spores in Kiwi Fruit Juice","volume":"10","author":"Zhang","year":"2017","journal-title":"Food Bioprocess Technol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.4315\/0362-028X-57.12.1080","article-title":"Growth Characteristics of Aciduric Sporeforming Bacilli Isolated from Fruit Juices","volume":"57","author":"Splittstoesser","year":"1994","journal-title":"J. Food Prot."},{"key":"ref_80","first-page":"321","article-title":"Effect of Nisin on the Physical and Chemical Characteristics of Apple Juice and the Control of","volume":"17","author":"Caregnato","year":"2014","journal-title":"Int. J. Sci."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1904","DOI":"10.4315\/0362-028X-69.8.1904","article-title":"Microbial Modeling of Alicyclobacillus acidoterrestris CRA 7152 Growth in Orange Juice with Nisin Added","volume":"69","year":"2006","journal-title":"J. Food Prot."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1111\/j.1745-4549.2010.00496.x","article-title":"Modeling the growth limit of Alicyclobacillus acidoterrestris CRA7152 in apple juice: Effect of pH, brix, temperature and nisin concentration","volume":"35","author":"Saraiva","year":"2011","journal-title":"J. Food Process. Preserv."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"e13051","DOI":"10.1111\/jfpp.13051","article-title":"The Effect of Nisin on the Growth and Heat Resistance of Alicyclobacillus acidoterrestris, Alicyclobacillus herbarius and Alicyclobacillus contaminans in Kiwi Juice: Control of Alicyclobacillus in kiwi fruit","volume":"41","author":"Jiangbo","year":"2017","journal-title":"J. Food Process. Preserv."},{"key":"ref_84","first-page":"61","article-title":"Synergistic Effect of Nisin and Cinnamaldehyde against Alicyclobacillus acidoterrestris in Orange Nectar","volume":"6","author":"Sobhy","year":"2015","journal-title":"Res. J. Pharm. Biol. Chem. Sci."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"315","DOI":"10.3934\/microbiol.2017.2.315","article-title":"Modeling Growth of Alicyclobacillus acidoterrestris DSM 3922 Type Strain Vegetative Cells in the Apple Juice with Nisin and Lysozyme","volume":"3","author":"Molva","year":"2017","journal-title":"AIMS Microbiol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"6712","DOI":"10.1039\/C6RA25860C","article-title":"Iron Oxide Nanoparticles Functionalized with Nisin for Rapid Inhibition and Separation of Alicyclobacillus spp","volume":"7","author":"Song","year":"2017","journal-title":"RSC Adv."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"109911","DOI":"10.1016\/j.lwt.2020.109911","article-title":"Effect of Ultraviolet Treatment (UV\u2013C) Combined with Nisin on Industrialized Orange Juice in Alicyclobacillus acidoterrestris Spores","volume":"133","author":"Ferreira","year":"2020","journal-title":"LWT"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1080\/08957959.2012.664642","article-title":"The Combined Effect of High Pressure and Nisin or Lysozyme on the Inactivation of Alicyclobacillus acidoterrestris Spores in Apple Juice","volume":"32","author":"Niezgoda","year":"2012","journal-title":"High Press. Res."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.foodcont.2013.03.040","article-title":"Primary Characterization of Bacteriocin Paracin C\u2014A Novel Bacteriocin Produced by Lactobacillus paracasei","volume":"34","author":"Pei","year":"2013","journal-title":"Food Control."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"e12350","DOI":"10.1111\/jfs.12350","article-title":"Activity of Paracin C from Lactic Acid Bacteria against Alicyclobacillus in Apple Juice: Application of a Novelty Bacteriocin","volume":"37","author":"Pei","year":"2017","journal-title":"J. Food Saf."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1111\/j.1365-2672.2007.03710.x","article-title":"Bovicin HC5 Reduces Thermal Resistance of Alicyclobacillus acidoterrestris in Acidic Mango Pulp","volume":"104","author":"Vanetti","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"3994","DOI":"10.1002\/jsfa.11747","article-title":"Bovicin HC5 and Nisin Reduce Cell Viability and the Thermal Resistance of Alicyclobacillus acidoterrestris Endospores in Fruit Juices","volume":"102","author":"Ribeiro","year":"2022","journal-title":"J. Sci. Food Agric."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1007\/s11274-013-1491-1","article-title":"Control of Alicyclobacillus acidoterrestris in Fruit Juices by a Newly Discovered Bacteriocin","volume":"30","author":"Pei","year":"2014","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1111\/jam.12145","article-title":"Characterization of Bacteriocin Bificin C6165: A Novel Bacteriocin","volume":"114","author":"Pei","year":"2013","journal-title":"J. Appl. Microbiol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.4315\/0362-028X.JFP-12-496","article-title":"Purification and Characterization of Anti-Alicyclobacillus Bacteriocin Produced by Lactobacillus rhamnosus","volume":"76","author":"Yue","year":"2013","journal-title":"J. Food Prot."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.ijfoodmicro.2005.03.010","article-title":"Control of Alicyclobacillus acidoterrestris in Fruit Juices by Enterocin AS-48","volume":"104","author":"Grande","year":"2005","journal-title":"Int. J. Food Microbiol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s00284-005-4456-2","article-title":"Purification and Characterization of Warnericin RB4, Anti-Alicyclobacillus Bacteriocin, Produced by Staphylococcus warneri RB4","volume":"51","author":"Minamikawa","year":"2005","journal-title":"Curr. Microbiol."},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Pei, J., Jin, W., Wang, J., Huang, Y., Li, X., Zhang, H., Zhang, Y., Ramadan, A., and El-Aty, A.M.A. (2021). Purification and Characterization of Plantaricin YKX and Assessment of Its Inhibitory Activity Against Alicyclobacillus spp. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.783266"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"100460","DOI":"10.1016\/j.jfp.2025.100460","article-title":"Control of Alicyclobacillus acidoterrestris in Apple Juice with Natural Antimicrobial Glycolipid","volume":"88","author":"Wang","year":"2025","journal-title":"J. Food Prot."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"3621","DOI":"10.1002\/fsn3.3360","article-title":"Bacteriophages and Food Safety: An Updated Overview","volume":"11","author":"Imran","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Shymialevich, D., W\u00f3jcicki, M., \u015awider, O., \u015arednicka, P., and Soko\u0142owska, B. (2023). Characterization and Genome Study of a Newly Isolated Temperate Phage Belonging to a New Genus Targeting Alicyclobacillus acidoterrestris. Genes, 14.","DOI":"10.3390\/genes14061303"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.ijfoodmicro.2008.02.030","article-title":"Alicyclobacillus acidoterrestris: New Methods for Inhibiting Spore Germination","volume":"125","author":"Bevilacqua","year":"2008","journal-title":"Int. J. Food Microbiol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1111\/j.1365-2621.2007.01604.x","article-title":"Inhibition of Alicyclobacillus acidoterrestris Spores by Natural Compounds","volume":"43","author":"Bevilacqua","year":"2008","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1111\/j.1472-765X.2004.01538.x","article-title":"Antimicrobial Activities of Eucalyptus Leaf Extracts and Flavonoids from Eucalyptus maculata","volume":"39","author":"Takahashi","year":"2004","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/j.foodcont.2012.12.014","article-title":"Control of Alicyclobacillus acidoterrestris in Apple Juice by Citrus Extracts and a Mild Heat-Treatment","volume":"31","author":"Bevilacqua","year":"2013","journal-title":"Food Control."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1016\/j.lwt.2015.02.021","article-title":"Evaluation of Bioactivity of Pomegranate Fruit Extract against Alicyclobacillus acidoterrestris DSM 3922 Vegetative Cells and Spores in Apple Juice","volume":"62","author":"Molva","year":"2015","journal-title":"LWT"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.lwt.2014.07.029","article-title":"Antimicrobial Activity of Grape Seed Extract on Alicyclobacillus acidoterrestris DSM 3922 Vegetative Cells and Spores in Apple Juice","volume":"60","author":"Molva","year":"2015","journal-title":"LWT"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.foodcont.2015.07.018","article-title":"Control of Alicyclobacillus spp. Vegetative Cells and Spores in Apple Juice with Rosemary Extracts","volume":"60","author":"Piskernik","year":"2016","journal-title":"Food Control."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.indcrop.2018.02.073","article-title":"Piperaceae Extracts for Controlling Alicyclobacillus acidoterrestris Growth in Commercial Orange Juice","volume":"116","author":"Lorenzetti","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.ijfoodmicro.2012.08.004","article-title":"Inactivation of Alicyclobacillus acidoterrestris in Orange Juice by Saponin Extracts Combined with Heat-Treatment","volume":"159","author":"Alberice","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"e16310917907","DOI":"10.33448\/rsd-v10i9.17907","article-title":"Antibacterial Activity of Crude Extract of Tabernaemontana catharinensis Latex (A. DC) against Alicyclobacillus spp","volume":"10","author":"Richard","year":"2021","journal-title":"Res. Soc. Dev."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"104597","DOI":"10.1016\/j.fm.2024.104597","article-title":"Comparative Assessment of Antibacterial Activity of Matricaria chamomilla L. Extract, Nisin and of Its Combination against Alicyclobacillus spp.","volume":"124","author":"Pretes","year":"2024","journal-title":"Food Microbiol."},{"key":"ref_113","doi-asserted-by":"crossref","unstructured":"Bevilacqua, A., Corbo, M.R., and Sinigaglia, M. (2011). Use of Essential Oils to Inhibit Alicyclobacillus acidoterrestris: A Short Overview of the Literature. Front. Microbiol., 2.","DOI":"10.3389\/fmicb.2011.00195"},{"key":"ref_114","doi-asserted-by":"crossref","unstructured":"Dawan, J., Zhang, S., and Ahn, J. (2025). Recent Advances in Biofilm Control Technologies for the Food Industry. Antibiotics, 14.","DOI":"10.3390\/antibiotics14030254"},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Al-Sharify, Z.T., Al-Najjar, S.Z., Naser, Z.A., Alsherfy, Z.A.I., and Onyeaka, H. (2025). The Impact of Fluid Flow on Microbial Growth and Distribution in Food Processing Systems. Foods, 14.","DOI":"10.3390\/foods14030401"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1007\/s42770-021-00624-x","article-title":"Microbial Biofilm: Formation, Architecture, Antibiotic Resistance, and Control Strategies","volume":"52","author":"Rather","year":"2021","journal-title":"Braz. J. Microbiol."},{"key":"ref_117","doi-asserted-by":"crossref","unstructured":"Perchikov, R., Cheliukanov, M., Plekhanova, Y., Tarasov, S., Kharkova, A., Butusov, D., Arlyapov, V., Nakamura, H., and Reshetilov, A. (2024). Microbial Biofilms: Features of Formation and Potential for Use in Bioelectrochemical Devices. Biosensors, 14.","DOI":"10.3390\/bios14060302"},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Blaschek, H.P., Wang, H.H., and Agle, M.E. (2007). Biofilms in the Food Environment, Blackwell Publishing. Institute of Food Technologists Series.","DOI":"10.1002\/9780470277782"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"1893","DOI":"10.1128\/aem.53.8.1893-1897.1987","article-title":"The Role of Bacterial Cell Wall Hydrophobicity in Adhesion","volume":"53","author":"Lyklema","year":"1987","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"881","DOI":"10.3201\/eid0809.020063","article-title":"Biofilms: Microbial Life on Surfaces","volume":"8","author":"Donlan","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.resmic.2005.01.007","article-title":"Quantitative Analysis of Adhesion and Biofilm Formation on Hydrophilic and Hydrophobic Surfaces of Clinical Isolates of Staphylococcus epidermidis","volume":"156","author":"Cerca","year":"2005","journal-title":"Res. Microbiol."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"785","DOI":"10.18388\/abp.2015_1133","article-title":"Evaluation of Hydrophobicity and Quantitative Analysis of Biofilm Formation by Alicyclobacillus sp.","volume":"62","author":"Tyfa","year":"2015","journal-title":"Acta Biochim. Pol."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00248-007-9245-y","article-title":"Evaluation of Adherence, Hydrophobicity, Aggregation, and Biofilm Development of Flavobacterium johnsoniae-Like Isolates","volume":"55","author":"Basson","year":"2008","journal-title":"Microb. Ecol."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1111\/j.1365-2672.2007.03688.x","article-title":"Influence of Temperature on Biofilm Formation by Listeria monocytogenes on Various Food-Contact Surfaces: Relationship with Motility and Cell Surface Hydrophobicity","volume":"104","author":"Piccolomini","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.4315\/0362-028X.JFP-13-020","article-title":"Resistance of Alicyclobacillus acidoterrestris Spores and Biofilm to Industrial Sanitizers","volume":"76","author":"Anjos","year":"2013","journal-title":"J. Food Prot."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"510","DOI":"10.4315\/0362-028X-72.3.510","article-title":"Destruction of Alicyclobacillus acidoterrestris Spores in Apple Juice on Stainless Steel Surfaces by Chemical Disinfectants","volume":"72","author":"Podolak","year":"2009","journal-title":"J. Food Prot."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.4315\/0362-028X.JFP-13-425","article-title":"External pH Is a Cue for the Behavioral Switch That Determines Surface Motility and Biofilm Formation of Alicyclobacillus acidoterrestris","volume":"77","author":"Shemesh","year":"2014","journal-title":"J. Food Prot."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"e20190790","DOI":"10.1590\/0103-8478cr20190790","article-title":"Contribution of Environmental Factors in the Formation of Biofilms by Alicyclobacillus acidoterrestris on Surfaces of the Orange Juice Industry","volume":"50","author":"Dutra","year":"2020","journal-title":"Cienc. Rural"},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.4315\/0362-028X-63.8.1117","article-title":"Efficacy of Disinfectants in Killing Spores of Alicyclobacillus acidoterrestris and Performance of Media for Supporting Colony Development by Survivors","volume":"63","author":"Orr","year":"2000","journal-title":"J. Food Prot."},{"key":"ref_130","doi-asserted-by":"crossref","unstructured":"Kunicka-Styczy\u0144ska, A., Tyfa, A., Laskowski, D., Pluci\u0144ska, A., Rajkowska, K., and Kowal, K. (2020). Clove Oil (Syzygium aromaticum L.) Activity against Alicyclobacillus acidoterrestris Biofilm on Technical Surfaces. 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