{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T18:59:22Z","timestamp":1768417162590,"version":"3.49.0"},"reference-count":47,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Project of Jilin Province","award":["JJKH20240246KJ"],"award-info":[{"award-number":["JJKH20240246KJ"]}]},{"name":"Natural Science Foundation that from Jilin Provincial Department of Science and Technology","award":["YDZJ20261ZYTS547"],"award-info":[{"award-number":["YDZJ20261ZYTS547"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Listeria monocytogenes (LM) is a lethal foodborne intracellular pathogen. Internalins A and B (inlA and inlB) are critical virulence factors that promote LM\u2019s adhesion and invasion into host cells. InlA is covalently anchored to the cell wall by LM SortaseA (SrtA), while inlB is anchored to the cell wall via non-covalent bonds. Therefore, inhibiting SrtA and inlB is expected to suppress LM\u2019s adhesion and invasion of host cells, enabling the prevention and control of infections. This study demonstrated that Luteolin inhibited the activity of purified LM SrtA protein in vitro. Interactive mechanism analysis indicated that Luteolin generates interaction with the critical active sites of SrtA, which may affect its binding to its natural substrates, thereby reducing the anchoring of inlA on the cell wall and achieving the inhibition of bacterial adhesion and invasion. In addition, Luteolin binds to the groove at the binding interface between inlB and its host receptor. The key residues in inlB that interact with the host receptor form weak interactions (Hydrogen bonds and van der Waals interactions) with Luteolin, this binding may inhibit their binding, suppressing LM\u2019s adhesion and invasion of host cells. At the tested concentrations, Luteolin did not affect the growth of LM, but remarkably reduced the mortality and alleviated the infection symptoms of LM-infected Galleria mellonella. These results provide additional theoretical evidence for the application of Luteolin in the prevention and control of LM infections, which is expected to accelerate its application progress.<\/jats:p>","DOI":"10.3390\/molecules31020297","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T11:01:14Z","timestamp":1768388474000},"page":"297","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Luteolin Inhibits Invasion of Listeria monocytogenes by Interacting with SortaseA and InternalinB"],"prefix":"10.3390","volume":"31","author":[{"given":"Junlu","family":"Liu","sequence":"first","affiliation":[{"name":"College of Biological and Food Engineering, Jilin Engineering Normal University, Changchun 130052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering, Jilin Engineering Normal University, Changchun 130052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang","family":"Pan","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering, Jilin Engineering Normal University, Changchun 130052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahui","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering, Jilin Engineering Normal University, Changchun 130052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiong","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering, Jilin Engineering Normal University, Changchun 130052, China"},{"name":"Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guizhen","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering, Jilin Engineering Normal University, Changchun 130052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1007\/s00248-023-02269-9","article-title":"Listeria monocytogenes an Emerging Pathogen: A Comprehensive Overview on Listeriosis, Virulence Determinants, Detection, and Anti-Listerial Interventions","volume":"86","author":"Ravindhiran","year":"2023","journal-title":"Microb. 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