{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T18:05:44Z","timestamp":1768586744998,"version":"3.49.0"},"reference-count":28,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82160671"],"award-info":[{"award-number":["82160671"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Endophytic FoRo54 was isolated from the roots of Oryza rufipogon (Dongxiang wild rice) collected in China. Based on morphological characteristics and phylogenetic analysis of the 16S rRNA gene sequence, strain FoRo54 was identified as closely related to Micromonospora rosaria. The complete genome of FoRo54 consists of a linear chromosome of 7,057,852 bp with a GC content of 73.8 mol%. Genome mining using antiSMASH revealed 27 biosynthetic gene clusters (BGCs) potentially involved in secondary metabolite biosynthesis, including those associated with kanamycin, rosamicin, and asukamycin, consistent with the antibacterial activities of the strain. Application of a combined genome mining strategy enabled further exploration of the strain\u2019s metabolic potential. One new rosamicin derivative, N-demethyl rosamicin (1), together with three known compounds, rosamicin (2), SCH 23831 (3), and tylactone (4), were isolated from fermentation broth. Antibacterial evaluation revealed that compounds 1-4 exhibited potent inhibitory activity against Staphylococcus aureus. Furthermore, based on genomic analysis, the biosynthetic pathway and putative gene functions responsible for these metabolites were proposed. Collectively, these findings highlight the metabolic versatility of the endophytic Micromonospora rosaria FoRo54, underscoring its potential as a valuable source of novel bioactive metabolites and providing a genomic framework for future heterologous expression and functional genetic characterization.<\/jats:p>","DOI":"10.3390\/molecules31020301","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T15:12:04Z","timestamp":1768403524000},"page":"301","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Discovery of a New Rosamicin Derivative from Endophytic Micromonospora\u00a0rosaria FoRo54 Using Genome Mining Technology"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9814-6686","authenticated-orcid":false,"given":"Zhi-Bin","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Biodiversity Conservation and Bioresource Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang 330022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6061-2970","authenticated-orcid":false,"given":"Qi","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biodiversity Conservation and Bioresource Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang 330022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Dong","family":"Song","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biodiversity Conservation and Bioresource Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang 330022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Wen","family":"Xiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Microbial Medicine Research of Jiangxi Province, College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ri-Ming","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biodiversity Conservation and Bioresource Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang 330022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Du","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biodiversity Conservation and Bioresource Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang 330022, China"},{"name":"Key Laboratory of Natural Microbial Medicine Research of Jiangxi Province, College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/s43141-021-00156-9","article-title":"Secondary metabolites and biodiversity of actinomycetes","volume":"19","author":"Selim","year":"2021","journal-title":"J. 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