{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T06:04:43Z","timestamp":1773900283469,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T00:00:00Z","timestamp":1768608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Minist\u00e8re de l\u2019Education Nationale et de la Recherche"},{"name":"Collectivit\u00e9s Locales Mayennaises"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Non-alcoholic fatty liver disease (NAFLD), often associated with obesity, has become a serious public health matter. NAFLD is characterized by an excessive lipid accumulation in hepatocytes, mainly stored as triglycerides. The marine microalga Phaeodactylum tricornutum is well known for its richness of bioactive compounds, particularly lipids. Therefore, different natural lipid extracts from P. tricornutum are deciphered to jugulate or prevent obesity leading to NAFLD. In this study, the main focus was on the effects of purified neutral and polar lipid extracts from P. tricornutum in a cellular model of NAFLD. Human HepG2 cells were used and exposed for 24 h to 250 \u03bcM palmitate to induce NAFLD with or without microalgal lipid extracts. Data showed that neutral lipid extract presented lower viability and cytotoxic activities on HepG2 at 75 \u00b5g\/mL. The impact on apoptosis was around 5% and below the threshold. Nevertheless, the use of neutral lipid at 50 \u00b5g\/mL induced a decrease in the number and size of lipid droplets, and so, preventing NAFLD. On the contrary, the polar lipid extract had no effect on the accumulation of triglycerides in HepG2 cells. To conclude, neutral lipid extract seemed to be a good candidate to prevent NAFLD.<\/jats:p>","DOI":"10.3390\/molecules31020323","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T09:57:02Z","timestamp":1768816622000},"page":"323","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Protective Effects of Neutral Lipids from Phaeodactylum tricornutum on Palmitate-Induced Lipid Accumulation in HepG2 Cells: An In Vitro Model of Non-Alcoholic Fatty Liver Disease"],"prefix":"10.3390","volume":"31","author":[{"given":"Marion","family":"Peyras","sequence":"first","affiliation":[{"name":"Biology of Organisms, Stress, Health and Environment, D\u00e9partement G\u00e9nie Biologique, Institut Universitaire de Technologie, Le Mans Universit\u00e9, IUML-FR 3473 CNRS, F-53020 Laval CEDEX 9, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rose-Marie","family":"Orhant","sequence":"additional","affiliation":[{"name":"Biology of Organisms, Stress, Health and Environment, D\u00e9partement G\u00e9nie Biologique, Institut Universitaire de Technologie, Le Mans Universit\u00e9, IUML-FR 3473 CNRS, F-53020 Laval CEDEX 9, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4646-6036","authenticated-orcid":false,"given":"Giuliana","family":"Parisi","sequence":"additional","affiliation":[{"name":"Department of Agriculture Food Environment and Forestry, University of Florence, Via delle Cascine 5, I-50144 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7635-1075","authenticated-orcid":false,"given":"Cecilia","family":"Faraloni","sequence":"additional","affiliation":[{"name":"Institute for BioEconomy, Department of Biology, Agriculture and Food Sciences, National Research Council, Sesto Fiorentino, I-50019 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graziella","family":"Chini Zittelli","sequence":"additional","affiliation":[{"name":"Institute for BioEconomy, Department of Biology, Agriculture and Food Sciences, National Research Council, Sesto Fiorentino, I-50019 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Blanckaert","sequence":"additional","affiliation":[{"name":"Biology of Organisms, Stress, Health and Environment, D\u00e9partement G\u00e9nie Biologique, Institut Universitaire de Technologie, Le Mans Universit\u00e9, IUML-FR 3473 CNRS, F-53020 Laval CEDEX 9, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2468-0659","authenticated-orcid":false,"given":"Virginie","family":"Mimouni","sequence":"additional","affiliation":[{"name":"Biology of Organisms, Stress, Health and Environment, D\u00e9partement G\u00e9nie Biologique, Institut Universitaire de Technologie, Le Mans Universit\u00e9, IUML-FR 3473 CNRS, F-53020 Laval CEDEX 9, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1016\/j.fertnstert.2017.02.104","article-title":"Obesity Pandemic: Causes, Consequences, and Solutions\u2014But Do We Have the Will?","volume":"107","author":"Meldrum","year":"2017","journal-title":"Fertil. 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