{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T04:50:44Z","timestamp":1782363044643,"version":"3.54.5"},"reference-count":58,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Calbee, Inc., Tokyo, Japan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Sweet potatoes (Ipomoea batatas (L.) Lam.) are known for their anti-inflammatory effects, which are attributed to their phytochemical content. Our previous study revealed that ethanol extracts of sweet potato storage roots (SP-EtOH-Ex) inhibit interleukin-6 (IL-6) production in RAW264.7 cells stimulated with lipopolysaccharide (LPS). However, the causative compounds responsible for the anti-inflammatory effect have not yet been identified. This study aims to identify the compounds responsible for the anti-inflammatory effect of SP-EtOH-Ex using liquid chromatography\u2013quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). The unknown compounds were measured using the auto MS\/MS mode (data-dependent acquisition; DDA) of LC-Q-TOF-MS, and the resulting data were analyzed using MS-DIAL and MS-FINDER and also compared with those of the corresponding reference standards in terms of retention time and fragment ions. As a result, \u03b2-sitosterol (2.527\u20134.850 \u00b5g\/mL), campesterol (75.74\u201393.63 ng\/mL), and lauroyl diethanolamide (4.568\u20139.260 ng\/mL) were identified and quantified in SP-EtOH-Ex. Moreover, the anti-inflammatory effect of these three compounds against RAW264.7 cells was investigated at varying concentrations of \u03b2-sitosterol (1 \u00b5g\/mL, 5 \u00b5g\/mL, 10 \u00b5g\/mL), campesterol (10 ng\/mL, 100 ng\/mL, 1000 ng\/mL), and lauroyl diethanolamide (1 ng\/mL, 10 ng\/mL, 100 ng\/mL). The phytosterols \u03b2-sitosterol and campesterol suppressed LPS-induced IL-6 production at concentrations comparable to those present in SP-EtOH-Ex. In contrast, lauroyl diethanolamide did not similarly suppress LPS-induced IL-6 production. These results suggest that \u03b2-sitosterol and campesterol in sweet potato storage roots contribute to their anti-inflammatory effects. The lack of activity in lauroyl diethanolamide further supports that phytosterols are the primary anti-inflammatory constituents. The edible portion of sweet potatoes holds promise as a promising raw material with anti-inflammatory properties.<\/jats:p>","DOI":"10.3390\/molecules31030456","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T15:56:12Z","timestamp":1769615772000},"page":"456","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of Active Anti-Inflammatory Compounds in Sweet Potato Storage Roots Extracted with Ethanol via LC-Q-TOF-MS"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3677-7609","authenticated-orcid":false,"given":"Ryohei","family":"Sakuraoka","sequence":"first","affiliation":[{"name":"Research and Development Division, Calbee, Inc., Utsunomiya 321-3231, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9855-1973","authenticated-orcid":false,"given":"Hirofumi","family":"Masutomi","sequence":"additional","affiliation":[{"name":"Research and Development Division, Calbee, Inc., Utsunomiya 321-3231, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuyuki","family":"Ishihara","sequence":"additional","affiliation":[{"name":"Research and Development Division, Calbee, Inc., Utsunomiya 321-3231, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102208","DOI":"10.1016\/j.fbio.2022.102208","article-title":"Nutrition-related health outcomes of sweet potato (Ipomoea batatas) consumption: A systematic review","volume":"50","author":"Qin","year":"2022","journal-title":"Food Biosci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1016\/j.foodchem.2015.12.057","article-title":"Carotenoids and \u03b2-carotene in orange fleshed sweet potato: A possible solution to vitamin A deficiency","volume":"199","author":"Islam","year":"2016","journal-title":"Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Chen, C.C., Lin, C., Chen, M.H., and Chiang, P.Y. 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