{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:02:40Z","timestamp":1784127760349,"version":"3.55.0"},"reference-count":22,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Baise Science and Technology Plan Project","award":["BG252901"],"award-info":[{"award-number":["BG252901"]}]},{"name":"Joint Special Project of Guangxi Science and Technology Plan","award":["2025JJH140224"],"award-info":[{"award-number":["2025JJH140224"]}]},{"name":"Baise Medical and Health High-level Talent Research Project","award":["RZ2400001367"],"award-info":[{"award-number":["RZ2400001367"]}]},{"name":"Guangxi University Young and Middle-Aged Teachers\u2019 Scientific Research Basic Ability Improvement Project","award":["2025KY0575"],"award-info":[{"award-number":["2025KY0575"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Paris polyphylla (Chonglou), a medicinal herb documented in Shennong\u2019s Classic of Materia Medica and a key component of formulas such as Yunnan Baiyao, is a rare and endangered plant prized for its bioactive steroidal saponins, notably polyphyllin I (PPI) and II (PPII). However, its pharmacological potential is hampered by inefficient extraction and unreliable compound identification. Herein, we developed a sustainable and efficient extraction strategy using ultrasound-assisted deep eutectic solvents (DES), optimized via an L9(34) orthogonal experimental design. Extraction efficiencies across the seven Paris species ranged from 2.04% to 16.51%, achieved by systematically optimizing key parameters such as the choline chloride-to-ethanol molar ratio (1:1.8), material-to-liquid ratio (1:20 g mL\u22121), and extraction time (100 min). By ultra-high-performance liquid chromatography\u2013tandem mass spectrometry (UHPLC-MS\/MS) analysis, PPI and PPII were quantified using specific retention times and characteristic fragment ions, revealing content ranges of 3.282\u201321.452 mg g\u22121 and 4.201\u201317.975 mg g\u22121, respectively. This methodology provides a robust platform for quality control and standardization of Paris-derived medicines, while paving the way for sustainable utilization and in-depth study of its steroidal saponins.<\/jats:p>","DOI":"10.3390\/molecules31030473","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T12:49:07Z","timestamp":1769690947000},"page":"473","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Composition Study of Polyphyllin in Paris polyphylla by Ultrasound-Assisted Deep Eutectic Solvent Extraction Combined with UHPLC-MS\/MS"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4006-1683","authenticated-orcid":false,"given":"Jinyu","family":"Guo","sequence":"first","affiliation":[{"name":"School of Pharmacy, Youjiang Medical University for Nationalities, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajia","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Youjiang Medical University for Nationalities, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minlong","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Youjiang Medical University for Nationalities, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenlin","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Youjiang Medical University for Nationalities, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huayin","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Youjiang Medical University for Nationalities, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Youjiang Medical University for Nationalities, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"114455","DOI":"10.1016\/j.phytochem.2025.114455","article-title":"Antitumor activity and mechanistic study of steroidal saponins from the rhizomes of Paris polyphylla var. yunnanensis","volume":"235","author":"Zhang","year":"2025","journal-title":"Phytochemistry"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zhou, J., Liao, B., Miao, J., and Chen, X. 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