{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T01:22:11Z","timestamp":1775179331905,"version":"3.50.1"},"reference-count":50,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Dietary bioactive compounds are increasingly explored as complementary cardioprotective strategies, and the nitration of unsaturated fatty acids has emerged as a process capable of enhancing antiplatelet properties. This study investigated whether Phaseolus vulgaris L. extracts can generate nitrated fatty acids under gastric-like conditions and evaluated their effects on human platelet function. Bean extracts and major fatty acids were nitrated in vitro and tested using washed platelets to assess cytotoxicity, TRAP-6 and collagen-induced aggregation, activation markers (P-selectin, CD63), and mitochondrial responses including membrane potential, ROS production, and Ca2+ dynamics. Nitrated extracts markedly inhibited TRAP-6 induced aggregation (IC50 \u2248 1.8 mg\/mL), whereas non-nitrated extracts showed minimal activity; this effect was reversed by \u03b2-mercaptoethanol, indicating dependence on electrophilic nitroalkenes. Fractionation revealed that the lipidic fraction accounted for most of the antiplatelet effect, and isolated nitrated fatty acids (NO2-LN, NO2-LA, NO2-OA) displayed stronger inhibition than their native counterparts without increasing cytotoxicity. Nitrated species additionally reduced mitochondrial membrane potential and granule secretion without elevating ROS. These findings identify Phaseolus vulgaris L. as a natural source of bioactive nitrated fatty acids and support their potential as nutraceutical agents capable of modulating platelet activation and contributing to cardiovascular risk reduction.<\/jats:p>","DOI":"10.3390\/molecules31030488","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T11:09:22Z","timestamp":1769771362000},"page":"488","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Enhanced Antiplatelet Activity of Nitrated Fatty Acid Extracts from Phaseolus vulgaris L."],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1169-3805","authenticated-orcid":false,"given":"Lyanne","family":"Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Thrombosis and Healthy Aging Research Center, VITALIS Longevity Center, Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Medical Technology School, Universidad de Talca, Talca 3460000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6364-1356","authenticated-orcid":false,"given":"H\u00e9ctor Leonardo","family":"Montecino-Garrido","sequence":"additional","affiliation":[{"name":"Centro de Estudios en Alimentos Procesados-CEAP, Conicyt, Programa Regional R20F0001, Gore Maule, Talca 3460000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4108-8612","authenticated-orcid":false,"given":"Felipe","family":"Lagos","sequence":"additional","affiliation":[{"name":"Thrombosis and Healthy Aging Research Center, VITALIS Longevity Center, Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Medical Technology School, Universidad de Talca, Talca 3460000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basilio","family":"Carrasco","sequence":"additional","affiliation":[{"name":"Centro de Estudios en Alimentos Procesados-CEAP, Conicyt, Programa Regional R20F0001, Gore Maule, Talca 3460000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9618-8778","authenticated-orcid":false,"given":"Iv\u00e1n","family":"Palomo","sequence":"additional","affiliation":[{"name":"Thrombosis and Healthy Aging Research Center, VITALIS Longevity Center, Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Medical Technology School, Universidad de Talca, Talca 3460000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1160-8463","authenticated-orcid":false,"given":"Paulina","family":"Ormazabal","sequence":"additional","affiliation":[{"name":"Escuela de Obstetricia, Facultad de Ciencias para el Cuidado de la Salud, Universidad San Sebasti\u00e1n, Lota 2465, Providencia, Santiago 8420524, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9721-7577","authenticated-orcid":false,"given":"Andr\u00e9s","family":"Trostchansky","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica and Centro de Investigaciones Biom\u00e9dicas (CEINBIO), Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo 11200, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0099-4108","authenticated-orcid":false,"given":"Eduardo","family":"Fuentes","sequence":"additional","affiliation":[{"name":"Thrombosis and Healthy Aging Research Center, VITALIS Longevity Center, Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Medical Technology School, Universidad de Talca, Talca 3460000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"101622","DOI":"10.1016\/j.cpcardiol.2023.101622","article-title":"Global Cardiovascular Diseases Death Rate Prediction","volume":"48","author":"Gaidai","year":"2023","journal-title":"Curr. 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