{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T17:21:05Z","timestamp":1780593665352,"version":"3.54.1"},"reference-count":35,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deanship of Scientific Research at Philadelphia University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Currently, \u03c9-3 polyunsaturated fatty acids (PUFAs), which have become popular as dietary supplements, are limited by a shortage in supply. Thus, finding safe, effective alternatives is crucial. Echium seed oil (ESO), rich in \u03b1-linolenic acid (ALA, 18:3\u03c9-3) and stearidonic acid (SDA, 18:4\u03c9-3), surpasses many other botanical seed oils. In this study, a pseudotargeted approach was applied to characterize the lipidomic profile of two unexplored Echium species from the Mediterranean region. Our findings established Echium glomeratum as a rich source of \u03c9-3 fatty acids (FAs), exceeding many other species in both quality and quantity. E. glomeratum possesses different FAs and triglyceride (TG) profiles compared to E. judaeum, with the \u03c9-3:\u03c9-6 ratio being 3.5 and 1.3, respectively. This corresponds to higher quantities of ALA (45.50%) and SDA (12.59%) in E. glomeratum. Triglycerides (TGs) comprise 93% of the total lipid content in ESO. This study also profiled the most abundant TGs (50\u201360 carbons) from both species through comprehensive assignment of the olefination patterns. The E. glomeratum oil profile, containing a higher \u03c9-3 PUFA concentration, was further screened for cytotoxic and antioxidant activities. Our preliminary results demonstrated that E. glomeratum ESO may significantly suppress colon cancer cell growth.<\/jats:p>","DOI":"10.3390\/molecules31030550","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T13:56:23Z","timestamp":1770213383000},"page":"550","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comprehensive Profiling of Triglycerides in Wild Eastern Mediterranean Echium Seed Oil Using Patern\u00f2\u2013B\u00fcchi Modulated Lipidomics"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1341-9340","authenticated-orcid":false,"given":"Manal","family":"Alhusban","sequence":"first","affiliation":[{"name":"School of Pharmacy, Philadelphia University, Amman 19392, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suha","family":"Telfah","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9242-4262","authenticated-orcid":false,"given":"Mohammad M.","family":"Al-Gharaibeh","sequence":"additional","affiliation":[{"name":"Department of Plant Production, Faculty of Agriculture, Jordan University of Science and Technology, Irbid 22110, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4823-0708","authenticated-orcid":false,"given":"Sanaa","family":"Bardaweel","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman 11942, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raghad","family":"Alkadri","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Philadelphia University, Amman 19392, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0469-3567","authenticated-orcid":false,"given":"Fang","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Oilseeds Processing of Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China"},{"name":"Hubei Hongshan Laboratory, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1006\/phrs.1999.0495","article-title":"Health benefits of docosahexaenoic acid (DHA)","volume":"40","author":"Horrocks","year":"1999","journal-title":"Pharmacol. 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