{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T06:02:47Z","timestamp":1771048967536,"version":"3.50.1"},"reference-count":31,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hunan Provincial Department of Education Science Research Project","award":["23B0988"],"award-info":[{"award-number":["23B0988"]}]},{"name":"Hunan Provincial Department of Education Science Research Project","award":["22B0396"],"award-info":[{"award-number":["22B0396"]}]},{"DOI":"10.13039\/501100004735","name":"Hunan Provincial Natural Science Foundation of China","doi-asserted-by":"crossref","award":["2025JJ80302"],"award-info":[{"award-number":["2025JJ80302"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"crossref"}]},{"name":"\u201cLiu Liang Academician Workstation\u201d Mentorship Program","award":["24YS004"],"award-info":[{"award-number":["24YS004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Triple-negative breast cancer (TNBC) is a highly aggressive subtype with limited targeted therapies, underscoring an urgent need for novel agents. The soil-derived Streptomyces sp. CB00271, isolated from a biodiversity hotspot, was investigated for its bioactive metabolites. Bioassay-guided isolation led to the identification of actinolactomycin (1), alongside daidzein (2) and genistein (3). Remarkably, actinolactomycin (1) exhibited potent cytotoxicity against TNBC models, with IC50 values of 0.72 \u00b1 0.12 \u03bcM (MDA-MB-231) and 0.15 \u00b1 0.02 \u03bcM (4T1), demonstrating approximately 9-fold and 31-fold greater potency than cisplatin, respectively, and suggesting action through targeted pathway inhibition. This study constitutes the first report to systematically highlight the exceptional anti-TNBC potential of this rare natural product, establishing it as a promising lead compound against this challenging subtype. Furthermore, Streptomyces sp. CB00271 is identified as a valuable and scarce microbial resource for actinolactomycin, providing a new avenue to address its supply limitation and facilitate future development.<\/jats:p>","DOI":"10.3390\/molecules31040631","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T14:06:16Z","timestamp":1770905176000},"page":"631","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Actinolactomycin from Streptomyces sp. CB00271: Isolation, Identification, and Potent Anti-Triple-Negative Breast Cancer"],"prefix":"10.3390","volume":"31","author":[{"given":"Bang","family":"Gong","sequence":"first","affiliation":[{"name":"College of Pharmacy, Hunan Vocational College of Science and Technology, Changsha 410004, China"},{"name":"Xiangya International Academy of Translational Medicine, Central South University, Changsha 410013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Pan","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan Vocational College of Science and Technology, Changsha 410004, China"},{"name":"Xiangya International Academy of Translational Medicine, Central South University, Changsha 410013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan Vocational College of Science and Technology, Changsha 410004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shujuan","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan Vocational College of Science and Technology, Changsha 410004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingnan","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan Vocational College of Science and Technology, Changsha 410004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Xiangya International Academy of Translational Medicine, Central South University, Changsha 410013, China"},{"name":"Hunan Province Sino-US International Joint Research Center for Therapeutic Drugs of Senile Degenerative Diseases, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.18632\/oncotarget.12284","article-title":"Triple-Negative Breast Cancer: Is There a Treatment on the Horizon?","volume":"8","author":"Yao","year":"2017","journal-title":"Oncotarget"},{"key":"ref_2","first-page":"137","article-title":"Molecular Mechanism and Targeted Therapy Options of Triple-Negative (ER, PgR, HER-2\/neu) Breast Cancer: Review","volume":"4","author":"Kandula","year":"2013","journal-title":"World J. 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