{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:11:27Z","timestamp":1770909087835,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005942","name":"Butantan Foundation","doi-asserted-by":"publisher","award":["001\/0708\/000.202\/2025"],"award-info":[{"award-number":["001\/0708\/000.202\/2025"]}],"id":[{"id":"10.13039\/501100005942","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005942","name":"Butantan Foundation","doi-asserted-by":"publisher","award":["001\/0708\/000.186\/2025"],"award-info":[{"award-number":["001\/0708\/000.186\/2025"]}],"id":[{"id":"10.13039\/501100005942","id-type":"DOI","asserted-by":"publisher"}]},{"name":"S\u00e3o Paulo State Research Foundation","award":["2013\/07467-1"],"award-info":[{"award-number":["2013\/07467-1"]}]},{"DOI":"10.13039\/501100002322","name":"Brazilian National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["472744\/2012-7"],"award-info":[{"award-number":["472744\/2012-7"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Peptidomic studies in HeLa cells identified the antitumoral activity of Pep5, and our group observed that coupling Pep5 to a carrier peptide (Cpp) provides antimicrobial potential. This study evaluated the antimicrobial activity of Pep5-Cpp and its derivatives (\u0394C3-Pep5-Cpp and \u0394N-Pep5-Cpp) and investigated their potential mechanisms of action. For Staphylococcus aureus, MIC values were 6.25 \u00b5M for Pep5-Cpp and \u0394C3-Pep5-Cpp and 12.5 \u00b5M for \u0394N-Pep5-Cpp. Against Candida albicans, Pep5-Cpp showed a MIC of 3.125 \u00b5M, while both derivatives presented 6.25 \u00b5M. For Aspergillus niger, MIC values were 1.56 \u00b5M for Pep5-Cpp and \u0394N-Pep5-Cpp, and 25 \u00b5M for \u0394C3-Pep5-Cpp. Pep5-Cpp interacted with S. aureus DNA, increased fluorescence in S. aureus, and showed no change in C. albicans. Only \u0394C3-Pep5-Cpp inhibited biofilm (&gt;50%). Microbicidal assays showed that high concentrations were needed to kill S. aureus (&gt;100 \u00b5M), while lower concentrations completely inhibited C. albicans (&lt;6.25 \u00b5M). In the hemolytic assay, the peptides at their MIC values did not induce measurable hemolysis. These findings indicate that Pep5-Cpp and its derivatives have antimicrobial potential and selectivity, supporting future pharmacological development.<\/jats:p>","DOI":"10.3390\/molecules31040634","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T14:06:16Z","timestamp":1770905176000},"page":"634","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pep5-Cpp, a Cyclin D2-Derived Antimicrobial"],"prefix":"10.3390","volume":"31","author":[{"given":"Bianca Silva","family":"Souto","sequence":"first","affiliation":[{"name":"Applied Toxinology Laboratory, Butantan Institute, Rua Rudolf Virchow 250, Butant\u00e3, S\u00e3o Paulo 05345-040, SP, Brazil"},{"name":"Paulista School of Medicine, Federal University of S\u00e3o Paulo, Rua Botucatu, 740, Vila Clementino, S\u00e3o Paulo 04023-062, SP, Brazil"},{"name":"Postgraduate Program of Molecular Biology, Federal University of S\u00e3o Paulo, Rua Tr\u00eas de Maio 100, 4th Floor, Vila Clementino, S\u00e3o Paulo 04044-020, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vit\u00f3ria Stephani","family":"Hasbahr","sequence":"additional","affiliation":[{"name":"Applied Toxinology Laboratory, Butantan Institute, Rua Rudolf Virchow 250, Butant\u00e3, S\u00e3o Paulo 05345-040, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e1rbara Ribeiro Louren\u00e7o","family":"da Silva","sequence":"additional","affiliation":[{"name":"Applied Toxinology Laboratory, Butantan Institute, Rua Rudolf Virchow 250, Butant\u00e3, S\u00e3o Paulo 05345-040, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thais Lemos","family":"de Mattos","sequence":"additional","affiliation":[{"name":"Applied Toxinology Laboratory, Butantan Institute, Rua Rudolf Virchow 250, Butant\u00e3, S\u00e3o Paulo 05345-040, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Souza","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Applied Toxinology Laboratory, Butantan Institute, Rua Rudolf Virchow 250, Butant\u00e3, S\u00e3o Paulo 05345-040, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0218-9372","authenticated-orcid":false,"given":"Cec\u00edlia Mari","family":"Abe","sequence":"additional","affiliation":[{"name":"Bacteriology Laboratory, Butantan Institute, Rua Em\u00edlio Ribas 1058, Butant\u00e3, S\u00e3o Paulo 05503-100, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0654-639X","authenticated-orcid":false,"given":"Marcia Regina","family":"Franzolin","sequence":"additional","affiliation":[{"name":"Bacteriology Laboratory, Butantan Institute, Rua Em\u00edlio Ribas 1058, Butant\u00e3, S\u00e3o Paulo 05503-100, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6619-6489","authenticated-orcid":false,"given":"Pedro Ismael","family":"da Silva Junior","sequence":"additional","affiliation":[{"name":"Applied Toxinology Laboratory, Butantan Institute, Rua Rudolf Virchow 250, Butant\u00e3, S\u00e3o Paulo 05345-040, SP, Brazil"},{"name":"Paulista School of Medicine, Federal University of S\u00e3o Paulo, Rua Botucatu, 740, Vila Clementino, S\u00e3o Paulo 04023-062, SP, Brazil"},{"name":"Postgraduate Program of Molecular Biology, Federal University of S\u00e3o Paulo, Rua Tr\u00eas de Maio 100, 4th Floor, Vila Clementino, S\u00e3o Paulo 04044-020, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3285-306X","authenticated-orcid":false,"given":"Vanessa","family":"Rioli","sequence":"additional","affiliation":[{"name":"Applied Toxinology Laboratory, Butantan Institute, Rua Rudolf Virchow 250, Butant\u00e3, S\u00e3o Paulo 05345-040, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"108570","DOI":"10.1016\/j.biotechadv.2025.108570","article-title":"Advances in antimicrobial peptides: From mechanistic insights to chemical modifications","volume":"81","author":"Yang","year":"2025","journal-title":"Biotechnol. 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