{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T18:57:32Z","timestamp":1783105052634,"version":"3.54.6"},"reference-count":32,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Molecules"],"abstract":"<jats:p>Solid lipid nanoparticles (SLNs) are submicron colloidal systems widely investigated as drug carriers; however, their intrinsic biodistribution properties are also critical when SLNs are considered for diagnostic imaging. In the present proof-of-concept study, drug-free SLNs were evaluated exclusively as a radiolabeled imaging agent rather than as a drug delivery system. SLNs were radiolabeled with Technetium-99m (99mTc), and their in vivo biodistribution was investigated using gamma camera imaging, ex vivo organ counting, and confocal microscopy. SLNs were prepared by a microemulsion\u2013low-temperature solidification method and characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC). Radiolabeling efficiency was determined by instant thin-layer chromatography (ITLC) and exceeded 95%. Following intravenous administration in a rabbit model, dynamic scintigraphic imaging demonstrated predominant uptake in the liver and spleen. These findings were quantitatively confirmed by ex vivo biodistribution analysis at 4 h post-injection and qualitatively supported by confocal microscopy of liver and spleen tissues. The results indicate that 99mTc-labeled SLNs behave as RES-targeting radiocolloids and may serve as potential agents for liver\u2013spleen scintigraphy.<\/jats:p>","DOI":"10.3390\/molecules31040654","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:51:32Z","timestamp":1771005092000},"page":"654","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Biodistribution of Tc-99m-Labeled Solid Lipid Nanoparticles and Evaluation of Their Possibility as a Radiopharmaceutical"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8197-4162","authenticated-orcid":false,"given":"Hayrettin","family":"Eroglu","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Engineering and Architecture, Erzurum Technical University, 25110 Erzurum, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arif K\u00fcrsad","family":"Ayan","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, Samsun Medicana Hospital, 55100 Samsun, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayse","family":"Yenilmez","sequence":"additional","affiliation":[{"name":"Department of Mathematics and Science Education, Faculty of Education, Van Y\u00fcz\u00fcnc\u00fc Y\u0131l University, 65080 Van, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1166\/jbn.2016.2286","article-title":"An Investigation of the Usability of Solid Lipid Nanoparticles Radiolabelled with Tc-99m as Imaging Agents in Liver-Spleen Scintigraphy","volume":"12","author":"Eroglu","year":"2016","journal-title":"J. Biomed. Nanotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1016\/j.addr.2006.09.011","article-title":"Nanomedicine: Developing Smarter Therapeutic and Diagnostic Modalities","volume":"58","author":"Farokhzad","year":"2006","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"401","DOI":"10.2217\/17435889.3.4.401","article-title":"Impact of Nanoscience and Nanotechnology on Controlled Drug Delivery","volume":"3","author":"Youan","year":"2008","journal-title":"Nanomedicine"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.msec.2017.02.114","article-title":"Evaluation of radiolabeled curcumin-loaded solid lipid nanoparticles usage as an imaging agent in liver-spleen scintigraphy","volume":"75","author":"Ayan","year":"2017","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"112328","DOI":"10.1016\/j.inoche.2024.112328","article-title":"Research for a new imaging agent: In vivo examination of silymarin loaded solid lipid nanoparticles radiolabeled with 99mTc in rabbits","volume":"163","author":"Eroglu","year":"2024","journal-title":"Inorg. Chem. Commun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1517\/17425240902932908","article-title":"Multifunctional Nanoparticles for Imaging, Delivery and Targeting in Cancer Therapy","volume":"6","author":"Gindy","year":"2009","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.jconrel.2010.04.004","article-title":"Tumor-Homing Multifunctional Nanoparticles for Cancer Theragnosis: Simultaneous Diagnosis, Drug Delivery, and Therapeutic Monitoring","volume":"146","author":"Kim","year":"2010","journal-title":"J. Control. Release"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.nantod.2009.07.001","article-title":"Molecular Imaging and Therapy of Cancer with Radiolabeled Nanoparticles","volume":"4","author":"Hong","year":"2009","journal-title":"Nano Today"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1631\/jzus.B1400131","article-title":"Therapeutic Radionuclides in Nuclear Medicine: Current and Future Prospects","volume":"15","author":"Yeong","year":"2014","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1166\/jbn.2016.2172","article-title":"Exploiting Nanotechnology for the Development of MicroRNA-Based Cancer Therapeutics","volume":"12","author":"Tyagi","year":"2016","journal-title":"J. Biomed. Nanotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1021\/bc100478k","article-title":"Novel Method to Label Solid Lipid Nanoparticles with 64Cu for Positron Emission Tomography Imaging","volume":"22","author":"Andreozzi","year":"2011","journal-title":"Bioconjug. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1080\/1061186021000054933","article-title":"Lymphatic Uptake of Pulmonary Delivered Radiolabelled Solid Lipid Nanoparticles","volume":"10","author":"Videira","year":"2002","journal-title":"J. Drug Target."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1007\/s10967-014-3258-z","article-title":"An Easy and Effective Method for Radiolabelling of Solid Lipid Nanoparticles","volume":"302","author":"Banerjee","year":"2014","journal-title":"J. Radioanal. Nucl. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.ijpharm.2013.03.046","article-title":"Proof of Concept Studies to Confirm the Delivery of Curcumin Loaded Solid Lipid Nanoparticles (C-SLNs) to Brain","volume":"448","author":"Kakkar","year":"2013","journal-title":"Int. J. Pharm."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"30","DOI":"10.2174\/157341308783591816","article-title":"Solid Lipid Nanoparticles (SLNs) as a Rising Tool in Drug Delivery Science: One Step up in Nanotechnology","volume":"4","author":"Rai","year":"2008","journal-title":"Curr. Nanosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.3109\/03639045.2014.909840","article-title":"Physicochemical Characterization Techniques for Solid Lipid Nanoparticles: Principles and Limitations","volume":"40","author":"Kathe","year":"2014","journal-title":"Drug Dev. Ind. Pharm."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1166\/jbn.2009.1042","article-title":"Lipid Nanomedicines for Anticancer Drug Therapy","volume":"5","author":"Campanero","year":"2009","journal-title":"J. Biomed. Nanotechnol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Shivananjegowda, M.G., Hani, U., Osmani, R.A.M., Alamri, A.H., Ghazwani, M., Alhamhoom, Y., Rahamathulla, M., Paranthaman, S., Gowda, D.V., and Siddiqua, A. (2023). Development and Evaluation of Solid Lipid Nanoparticles for the Clearance of A\u03b2 in Alzheimer\u2019s Disease. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15010221"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Renukuntla, J., Peterson-Sockwell, S., Clark, B.A., Godage, N.H., Gionfriddo, E., Bolla, P.K., and Boddu, S.H.S. (2023). Design and Preclinical Evaluation of Nicotine\u2013Stearic Acid Conjugate-Loaded Solid Lipid Nanoparticles for Transdermal Delivery: A Technical Note. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15041043"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Creteanu, A., Lisa, G., Vasile, C., Popescu, M.-C., Spac, A.F., and Tantaru, G. (2023). Development of Solid Lipid Nanoparticles for Controlled Amiodarone Delivery. Methods Protoc., 6.","DOI":"10.3390\/mps6050097"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Santonocito, D., Sarpietro, M.G., Castelli, F., Lauro, M.R., Torrisi, C., Russo, S., and Puglia, C. (2023). Development of Solid Lipid Nanoparticles as Dry Powder: Characterization and Formulation Considerations. Molecules, 28.","DOI":"10.3390\/molecules28041545"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Aldayel, T.S., Badran, M.M., Alomrani, H.A., AlFaris, N.A., Altamimi, Z.J., Alqahtani, S.A., Nasr, A.F., Ghaffar, S., and Orfali, R. (2023). Chitosan-Coated Solid Lipid Nanoparticles as an Efficient Avenue for Boosted Biological Activities of Aloe perryi: Antioxidant, Antibacterial, and Anticancer Potential. Molecules, 28.","DOI":"10.3390\/molecules28083569"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.2967\/jnumed.115.160994","article-title":"Nanoparticles for Cardiovascular Imaging and Therapeutic Delivery, Part 1: Compositions and Features","volume":"56","author":"Stendahl","year":"2015","journal-title":"J. Nucl. Med."},{"key":"ref_24","first-page":"167","article-title":"Kat\u0131 Lipit Nanopartik\u00fcller (SLN)","volume":"25","author":"Demirel","year":"2000","journal-title":"FABAD J. Pharm. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1517\/17425247.2014.935335","article-title":"Compritol 888 ATO: A Multifunctional Lipid Excipient in Drug Delivery Systems and Nanopharmaceuticals","volume":"11","author":"Aburahma","year":"2014","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.addr.2012.09.021","article-title":"Solid lipid nanoparticles: Production, characterization and applications","volume":"64","author":"Mehnert","year":"2012","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Danaei, M., Dehghankhold, M., Ataei, S., Hasanzadeh Davarani, F., Javanmard, R., Dokhani, A., Khorasani, S., and Mozafari, M.R. (2018). Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems. Pharmaceutics, 10.","DOI":"10.3390\/pharmaceutics10020057"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1524","DOI":"10.1080\/10717544.2021.1951895","article-title":"Brain targeting efficiency of intranasal clozapine-loaded mixed micelles following radiolabeling with Technetium-99m","volume":"28","author":"Sayed","year":"2021","journal-title":"Drug Deliv."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1186\/s12951-022-01343-7","article-title":"The interaction between nanoparticles and immune system: Application in the treatment of inflammatory diseases","volume":"20","author":"Liu","year":"2022","journal-title":"J. Nanobiotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Hallan, S.S., Ferrara, F., Cortesi, R., and Sguizzato, M. (2025). Potential of the nano-encapsulation of antioxidant molecules in wound healing applications: An innovative strategy to enhance the bio-profile. Molecules, 30.","DOI":"10.3390\/molecules30030641"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Britto, N.T., Montanheri, L.R., Pelin, J.N., Siqueira, R.A., Alves, M.D.S., Martins, T.S., Hamley, I.W., Lopes, P.S., Leite-Silva, V.R., and Andreo-Filho, N. (2026). Development and Optimization of Resveratrol-Loaded NLCs via Low-Energy Methods: A Promising Alternative to Conventional High-Energy or Solvent-Based Techniques. Processes, 14.","DOI":"10.20944\/preprints202601.1115.v1"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Arroyo-Nogales, A., Plaza-Palomo, G., Gonz\u00e1lez-Larre, J., Jim\u00e9nez-Falcao, S., and Baeza, A. (2025). Silicasomes in Oncology: From Conventional Chemotherapy to Combined Immunotherapy. 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