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The accumulation of amyloid-\u03b2 (A\u03b2) aggregates on neuronal surfaces is a known important event that typifies AD. Although cell membrane architecture and cytoskeletal tension are thought to be involved in the process of A\u03b2 aggregation, it remains unclear how cytoskeleton-derived tension alters the function of cell membranes, which serve as a scaffold for A\u03b2 aggregation. In this study, we investigated whether cytoskeletal tension promotes A\u03b2 aggregation on neuroblastoma, SH-SY5Y cells. Cytoskeletal tension was enhanced by jasplakinolide, an actin depolymerization inhibitor, and calyculin A, a serine\/threonine phosphatase inhibitor that promotes myosin II activation. Real-time imaging with quantum-dot-labeled A\u03b2 nanoprobes revealed that both pharmacological treatments significantly increased A\u03b2 deposition on the surface of living cells. Our findings suggest that cytoskeletal tension promotes A\u03b2 aggregation over the membrane barrier, providing new insights into the biophysical mechanisms underlying A\u03b2 accumulation in AD pathogenesis.<\/jats:p>","DOI":"10.3390\/molecules31040718","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T13:47:02Z","timestamp":1771508822000},"page":"718","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhancement of Cytoskeletal Tension Promotes Amyloid-\u03b2 Aggregation on the Neuronal Cell Surface"],"prefix":"10.3390","volume":"31","author":[{"given":"Juri","family":"Nakayama","sequence":"first","affiliation":[{"name":"Graduate School of Engineering, Muroran Institute of Technology, Mizumoto 27-1, Muroran 050-8585, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuna","family":"Fujiya","sequence":"additional","affiliation":[{"name":"Graduate School of Engineering, Muroran Institute of Technology, Mizumoto 27-1, Muroran 050-8585, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7747-4808","authenticated-orcid":false,"given":"Kiyotaka","family":"Tokuraku","sequence":"additional","affiliation":[{"name":"Graduate School of Engineering, Muroran Institute of Technology, Mizumoto 27-1, Muroran 050-8585, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4958-1286","authenticated-orcid":false,"given":"Masahiro","family":"Kuragano","sequence":"additional","affiliation":[{"name":"Graduate School of Engineering, Muroran Institute of Technology, Mizumoto 27-1, Muroran 050-8585, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Breijyeh, Z., and Karaman, R. 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