{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T12:32:51Z","timestamp":1780921971205,"version":"3.54.1"},"reference-count":47,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2021YFD1600301"],"award-info":[{"award-number":["2021YFD1600301"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2021YFD1600105"],"award-info":[{"award-number":["2021YFD1600105"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Yuelushan Laboratory Breeding Program","award":["YLS-2025-ZY04057"],"award-info":[{"award-number":["YLS-2025-ZY04057"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Lilies (Lilium spp.) are highly valued in China for their edible and medicinal properties; however, bitterness in certain varieties limits consumer acceptance. Although historically attributed to colchicine, the presence of alkaloids in lilies remains a subject of debate. This research screened five lily species for colchicine and its 15 biosynthetic precursors, using Gloriosa superba and Colchicum autumnale as positive controls. While detected in the controls, none were detected in any tissues (bulbs, roots, stems, flowers, and leaves) of the five lilies. A comparative analysis of five lily varieties\u2014Longyahong, Lanzhou, Lilium lancifolium, Longya, and Guiyanghong\u2014revealed that Longyahong exhibited the strongest bitterness, which was localized exclusively in the bulb peels. Based on comparative LC-MS profiling between bitter and non-bitter varieties, three high-abundance compounds were selected for isolation and subsequent sensory evaluation. Two monomeric compounds were isolated and confirmed via chromatographic methods as the primary bitter components. This study provides compelling chemical and biochemical evidence of the presence of colchicine in the examined lilies. By identifying two specific bitter components in Longyahong bulb peels, these findings refute the long-standing misconception regarding colchicine in lilies and provide a chemical foundation for improving the palatability and commercial value of bitter lily varieties.<\/jats:p>","DOI":"10.3390\/molecules31040721","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T15:57:29Z","timestamp":1771516649000},"page":"721","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["LC-MS-Based Screening for Colchicine and Characterization of Major Bitter Constituents in Lily"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2895-7582","authenticated-orcid":false,"given":"Juhua","family":"Zhong","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yishuo","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faying","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyue","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewei","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbao","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haobo","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonghe","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguo","family":"Zeng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixing","family":"Qing","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"},{"name":"Changsha Changxu Biotechnology Ltd., Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"485","DOI":"10.4314\/ajtcam.v10i6.16","article-title":"A Study on the Extraction and Purification Process of Lily Polysaccharide and Its Anti-Tumor Effect","volume":"10","author":"Han","year":"2013","journal-title":"Afr. J. Trad. Compl. Alt. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"113852","DOI":"10.1016\/j.jep.2021.113852","article-title":"Genus Lilium: A Review on Traditional Uses, Phytochemistry and Pharmacology","volume":"270","author":"Zhou","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1093\/jpp\/rgae078","article-title":"Preparation, Characteristic, Biological Activities, and Application of Polysaccharide from Lilii Bulbus: A Review","volume":"76","author":"Duan","year":"2024","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/s41467-024-55545-8","article-title":"The Giant Genome of Lily Provides Insights into the Hybridization of Cultivated Lilies","volume":"16","author":"Liang","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_5","unstructured":"Chinese Pharmacopoeia Commission (2020). Pharmacopoeia of the People\u2019s Republic of China, China Medical Science Press. Part 1."},{"key":"ref_6","first-page":"785","article-title":"Research Advances in Lily Processing Technologits and Products","volume":"4","author":"Wu","year":"2025","journal-title":"J. Cold-Arid. Agric. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"105062","DOI":"10.1016\/j.jfca.2022.105062","article-title":"Geographical Origin and Species Identification of Lilii Bulbus Using C\/N\/H\/O Stable Isotopes and Multi-Elemental Combined Chemometrics","volume":"116","author":"Hai","year":"2023","journal-title":"J. Food Compos. Anal."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.fshw.2019.09.001","article-title":"A Critical Review on Chemical Constituents and Pharmacological Effects of Lilium","volume":"8","author":"Wang","year":"2019","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_9","first-page":"90","article-title":"Review: Colchicine, Current Advances and Future Prospects","volume":"2","author":"Ade","year":"2010","journal-title":"Nusant. Biosci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1016\/j.bse.2010.12.004","article-title":"Colchicine Is Still a Chemical Marker for the Expanded Colchicaceae","volume":"38","author":"Vinnersten","year":"2010","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_11","first-page":"1031","article-title":"Alkaloids and Phenolics of Wurmbea and Burchardia Species","volume":"26","author":"Macfarlane","year":"1986","journal-title":"Phytochemistry"},{"key":"ref_12","first-page":"1208","article-title":"Extraction and Anti-Inflammatory Effects of Colchicine from Sagittaria Sagittifolia","volume":"18","author":"Ou","year":"2017","journal-title":"Agric. Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"111788","DOI":"10.1016\/j.ejmech.2019.111788","article-title":"Recent Developments on (\u2212)-Colchicine Derivatives: Synthesis and Structure-Activity Relationship","volume":"185","author":"Ghawanmeh","year":"2020","journal-title":"Eur. J. Med. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1016\/j.clinthera.2022.06.009","article-title":"Efficacy of Colchicine in the Treatment of Patients With Coronary Artery Disease: A Mini-Review","volume":"44","author":"Yong","year":"2022","journal-title":"Clin. Ther."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.krcp.2013.04.003","article-title":"Colchicine-Induced Myoneuropathy in a Cyclosporine-Treated Renal Transplant Recipient","volume":"32","author":"Huh","year":"2013","journal-title":"Kidney Res. Clin. Pract."},{"key":"ref_16","first-page":"5","article-title":"Supercritical Fluid Extraction-High Performance Liquid Chromatography Determination of Colchicine in Lily","volume":"1","author":"He","year":"2003","journal-title":"Nat. Prod. Res. Dev."},{"key":"ref_17","first-page":"57","article-title":"Extraction and Antibacterial Activity of Colchicine from Lily Root Powder","volume":"32","author":"Chen","year":"2011","journal-title":"Food Sci. Technol."},{"key":"ref_18","first-page":"5","article-title":"Optimization of Ultrasonic-Assisted Extraction Process of Colchicine from Lily Flower by Response Surface Methodology","volume":"36","author":"Liu","year":"2015","journal-title":"Sci. Technol. Food Ind."},{"key":"ref_19","first-page":"1013","article-title":"Determination of Colchicine Content in Shaoyang Lilium Brownii var. Viridulum with Different Harvest Time by High Performance Liquid Chromatography","volume":"9","author":"Tang","year":"2018","journal-title":"J. Food Saf. Qual."},{"key":"ref_20","first-page":"78","article-title":"Determination of Colchicine by Secondary Derivative Polarography","volume":"23","author":"He","year":"2001","journal-title":"Nat. Sci. J. Xiangtan Univ."},{"key":"ref_21","unstructured":"He, S. (2002). Analysis and Research on Lilium Mid-Autumn Narcissine and Its Active Components, Central South University."},{"key":"ref_22","first-page":"122","article-title":"Analysis of Colchicine on Lilium Brownii from the Different Producing Areas and Lili Cloudy Juice","volume":"23","author":"Cheng","year":"2007","journal-title":"Food Mach."},{"key":"ref_23","first-page":"85","article-title":"Research on Debitterizing Technology of Lily","volume":"27","author":"Hu","year":"2015","journal-title":"Acta Agric. Jiangxi"},{"key":"ref_24","first-page":"529","article-title":"Determination of Colchicine and Optimization of Extraction Method in Lilium Brownii","volume":"27","author":"Yao","year":"2015","journal-title":"Clin. J. Tradit. Chin. Med."},{"key":"ref_25","unstructured":"He, C.L. (2003). Research on the Isolation and Application of Lilium Mid-Autumn Narcissine, Hunan University."},{"key":"ref_26","unstructured":"Li, G. (2004). Supercritical Fluid Extraction of Colchicine from Lilies, Central South University."},{"key":"ref_27","first-page":"36","article-title":"Determination of Colchicine Content in Colchicum Autumnale by Soxhlet Extraction-High Performance Liquid Chromatography","volume":"45","author":"Wang","year":"2024","journal-title":"South-Cent. Agric. Sci. Technol."},{"key":"ref_28","first-page":"85","article-title":"Determination of Colchicine Content in Colchicum Autumnale and Its Bulbs","volume":"39","author":"Chen","year":"2020","journal-title":"Chin. J. Tradit. Vet. Sci."},{"key":"ref_29","first-page":"286","article-title":"Separation and Identification of Colchicine from Lily","volume":"37","author":"He","year":"2006","journal-title":"J. Cent. South Univ. (Sci. Technol.)"},{"key":"ref_30","first-page":"37","article-title":"Study on Optimum Conditions of Supercritical Fluid Extraction of Colchicine from Lily","volume":"30","author":"He","year":"2003","journal-title":"J. Hunan Univ. (Nat. Sci.)"},{"key":"ref_31","first-page":"42","article-title":"Extraction of Colchicines from Bulbus Lilii with Ultrasonic","volume":"33","author":"Zhang","year":"2012","journal-title":"Food Ind."},{"key":"ref_32","first-page":"14","article-title":"Study on the Kinetics of Enzymatic Hydrolysis of Lily with \u03b1-Amylase and the Effect on Colchicine Extraction","volume":"31","author":"Huang","year":"2015","journal-title":"J. Guangdong Pharm. Univ."},{"key":"ref_33","first-page":"244","article-title":"Organic Solvent Extraction and Supercritical Fluid Extraction of Colchicine from Lily","volume":"35","author":"Li","year":"2004","journal-title":"J. Cent. South Univ. (Sci. Technol.)"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"143532","DOI":"10.1016\/j.foodchem.2025.143532","article-title":"Flavor Perception and Biological Activities of Bitter Compounds in Food","volume":"477","author":"Luo","year":"2025","journal-title":"Food Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1093\/chemse\/bjl052","article-title":"A Complex Relationship among Chemical Concentration, Detection Threshold, and Suprathreshold Intensity of Bitter Compounds","volume":"32","author":"Keast","year":"2007","journal-title":"Chem. Senses"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1002\/(SICI)1520-6505(1996)5:2<58::AID-EVAN5>3.0.CO;2-S","article-title":"Taste Perception and Feeding Behavior in Nonhuman Primates and Human Populations","volume":"5","author":"Hladik","year":"1996","journal-title":"Evol. Anthropol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"114778","DOI":"10.1016\/j.jpba.2022.114778","article-title":"Integrative Analysis of Metabolome and Transcriptome Provide New Insights into the Bitter Components of Lilium Lancifolium and Lilium Brownii","volume":"215","author":"Qin","year":"2022","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1080\/09168451.2018.1484273","article-title":"Tolerance of Bitter Stimuli and Attenuation\/Accumulation of Their Bitterness in Humans","volume":"82","author":"Mura","year":"2018","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"101426","DOI":"10.1016\/j.fochx.2024.101426","article-title":"Discovery of Bitter Masking Compounds from Allspice (Pimenta Dioica) Using Sensory Guided Isolation","volume":"22","author":"An","year":"2024","journal-title":"Food Chem. X"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/S0031-9422(99)00022-9","article-title":"Steroidal Saponins from the Bulbs of Lilium Candidum","volume":"51","author":"Mimaki","year":"1999","journal-title":"Phytochemistry"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"407","DOI":"10.3109\/15563650.2010.495348","article-title":"Colchicine Poisoning: The Dark Side of an Ancient Drug","volume":"48","author":"Finkelstein","year":"2010","journal-title":"Clin. Toxicol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5603","DOI":"10.1002\/jsfa.10613","article-title":"Structural Characterization of Lanzhou Lily (Lilium Davidii var. Unicolor) Polysaccharides and Determination of Their Associated Antioxidant Activity","volume":"100","author":"Li","year":"2020","journal-title":"J. Sci. Food Agric."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"146674","DOI":"10.1016\/j.foodchem.2025.146674","article-title":"Targeted Phytochemical Profiling and Functional Evaluation of Bitter and Sweet Lupinus Albus Genotypes from Diverse Origins","volume":"496","author":"Akremi","year":"2025","journal-title":"Food Chem."},{"key":"ref_44","first-page":"6173","article-title":"Evaluation of Hemp Tongue Sensation Grade and Prediction of Diester Alkaloid Content of Processed Products of Aconiti Kusnezoffii Radix Based on Electronic Tongue Bitterness Value","volume":"56","author":"Dege","year":"2025","journal-title":"Chin. Tradit. Herb. Drugs"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"103966","DOI":"10.1016\/j.fbio.2024.103966","article-title":"Sensory and Molecular Insights into the Bitterness of Wuyi Rock Tea","volume":"59","author":"Lu","year":"2024","journal-title":"Food Biosci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.foodqual.2006.05.002","article-title":"Food Quality Certification: An Approach for the Development of Accredited Sensory Evaluation Methods","volume":"18","author":"Elortondo","year":"2007","journal-title":"Food Qual. Prefer."},{"key":"ref_47","first-page":"65","article-title":"Research on Bitter Taste Analysis and Evaluation Technology for Soy Protein Isolate Using an Electronic Tongue","volume":"40","author":"Lu","year":"2019","journal-title":"J. Henan Univ. Technol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/4\/721\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T15:57:53Z","timestamp":1771516673000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/4\/721"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,19]]},"references-count":47,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["molecules31040721"],"URL":"https:\/\/doi.org\/10.3390\/molecules31040721","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,19]]}}}