{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:48:12Z","timestamp":1791290892836,"version":"4.3.0"},"reference-count":48,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["22276049"],"award-info":[{"award-number":["22276049"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Sustainable Development Demonstration Zone Project","award":["2022sfq49"],"award-info":[{"award-number":["2022sfq49"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Phytic acid (PA), owing to its strong acidity and multidentate metal-chelating properties, readily forms multiple adduct\/complex ions in mass spectrometry and is prone to pronounced matrix effects, resulting in complicated spectra and compromised sensitivity and quantitative robustness, which poses a major challenge for rapid and accurate PA quantification. Herein, we developed a rapid quantitative method for PA based on trimethylsilyldiazomethane (TMSD) methyl-ester derivatization coupled with paper spray mass spectrometry (PS\u2013MS). PA was derivatized with TMSD to yield the methylated product (PA-Me), and the derivative solution was purified via \u201cpost-derivatization nitrogen blow-down followed by water reconstitution\u201d, thereby markedly reducing background interference. In positive-ion mode, the stable sodium adduct ion [PA-Me+Na]+ (m\/z 851.04) was used as the quantifier, enabling fast quantification with selected ion monitoring (SIM). PS\u2013MS was performed with a 15 \u03bcL spotting volume and methanol\/water (90\/10, v\/v, containing 0.1% formic acid) as the spray solvent, allowing rapid analysis without chromatographic separation. The method exhibited good linearity over 0.125\u201330 \u03bcg\/mL (R2 \u2265 0.9965), with a limit of detection (LOD, S\/N = 3) of 0.080 \u03bcg\/mL and a limit of quantification (LOQ, S\/N = 10) of 0.270 \u03bcg\/mL. The intra-day and inter-day precision values were both &lt; 10% (RSD), and recoveries ranged from 87.2% to 122.4%. This LC-free strategy features low solvent consumption and high analytical throughput, and was validated using rice bran protein and rice bran polysaccharide samples, providing technical support for rapid screening and quality control of PA in complex food\/plant matrices.<\/jats:p>","DOI":"10.3390\/molecules31050799","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T17:05:16Z","timestamp":1772211916000},"page":"799","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Rapid Analysis of Phytic Acid by Paper Spray Mass Spectrometry"],"prefix":"10.3390","volume":"31","author":[{"given":"Ping","family":"Guo","sequence":"first","affiliation":[{"name":"Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, No. 36, Lushan Road, Changsha 410081, China"},{"name":"Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research of Ministry of Education, Hunan Normal University, No. 36, Lushan Road, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijie","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, No. 36, Lushan Road, Changsha 410081, China"},{"name":"Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research of Ministry of Education, Hunan Normal University, No. 36, Lushan Road, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, No. 36, Lushan Road, Changsha 410081, China"},{"name":"Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research of Ministry of Education, Hunan Normal University, No. 36, Lushan Road, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1111\/j.1365-2621.2009.01991.x","article-title":"Determination of phytic acid in cereals\u2014A brief review","volume":"44","author":"Wu","year":"2009","journal-title":"Int. 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