{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:14:52Z","timestamp":1790198092925,"version":"4.1.0"},"reference-count":71,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82405014"],"award-info":[{"award-number":["82405014"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004001","name":"Guizhou Science and Technology Department","doi-asserted-by":"crossref","award":["GMULH (2025) 019"],"award-info":[{"award-number":["GMULH (2025) 019"]}],"id":[{"id":"10.13039\/501100004001","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Qiankehe Foundation","award":["[2024] Youth 205"],"award-info":[{"award-number":["[2024] Youth 205"]}]},{"name":"Qiankehe Foundation","award":["MS [2025] 222"],"award-info":[{"award-number":["MS [2025] 222"]}]},{"name":"Qiankehe jichu","award":["ZK [2022] Yiban201"],"award-info":[{"award-number":["ZK [2022] Yiban201"]}]},{"name":"Xinzhiqianyan Leading Project of Guizhou Provincial Association for Science and Technology","award":["2025XZQYXM-01-09"],"award-info":[{"award-number":["2025XZQYXM-01-09"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Rosa roxburghii Tratt (RR) and Rosa kweichonensis var. sterilis (RS) are both edible medicinal plants. However, they are often confused due to their similar phenotypic characteristics, which may limit their targeted development and utilization. Here, we integrated targeted metabolomics (UPLC-MS\/MS) and transcriptomics (Illumina HiSeq) to systematically dissect the metabolic and transcriptional differences between the two species. Metabolomic profiling identified 558 differentially accumulated metabolites (DAMs), defined as metabolites with significantly different abundance between RS and RR, predominantly classified as flavonoids and phenolic acids. Among these, vitamin C (L-ascorbic acid) and argininosuccinic acid were prioritized as key DAMs based on their significant fold changes, high abundance, and functional relevance to bioactivity and stress tolerance. Transcriptomic analysis further revealed that vitamin C synthesis is primarily driven by the coordinated up-regulation of USP and GME genes in the L-ascorbic acid metabolic pathway, while argininosuccinic acid production, as an intermediate in the urea cycle, is mainly mediated by the up-regulated glnA gene. These findings not only clarify the molecular basis of metabolic divergence between RR and RS but also provide potential biomarkers for their identification, laying a solid foundation for their development as distinct functional foods.<\/jats:p>","DOI":"10.3390\/molecules31050831","type":"journal-article","created":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T16:17:31Z","timestamp":1772641051000},"page":"831","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative Metabolomics and Transcriptomics Analysis of Rosa roxburghii Tratt and Rosa kweichonensis var. sterilis"],"prefix":"10.3390","volume":"31","author":[{"given":"Xiaohui","family":"Wu","sequence":"first","affiliation":[{"name":"Key Laboratory of Minority Medical Resources Development and Utilization in Guizhou Province of State Ethnic Affairs Commission, Guizhou Minzu University, Guiyang 550025, China"},{"name":"School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Minority Medical Resources Development and Utilization in Guizhou Province of State Ethnic Affairs Commission, Guizhou Minzu University, Guiyang 550025, China"},{"name":"School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Minority Medical Resources Development and Utilization in Guizhou Province of State Ethnic Affairs Commission, Guizhou Minzu University, Guiyang 550025, China"},{"name":"School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zha","sequence":"additional","affiliation":[{"name":"Key Laboratory of Minority Medical Resources Development and Utilization in Guizhou Province of State Ethnic Affairs Commission, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoshun","family":"Pei","sequence":"additional","affiliation":[{"name":"School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yali","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Minority Medical Resources Development and Utilization in Guizhou Province of State Ethnic Affairs Commission, Guizhou Minzu University, Guiyang 550025, China"},{"name":"School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2552-1888","authenticated-orcid":false,"given":"Li","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Minority Medical Resources Development and Utilization in Guizhou Province of State Ethnic Affairs Commission, Guizhou Minzu University, Guiyang 550025, China"},{"name":"School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, China"},{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang 561113, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"108657","DOI":"10.1016\/j.pharmthera.2024.108657","article-title":"Chemical diversity, traditional uses, and bioactivities of Rosa roxburghii Tratt: A comprehensive review","volume":"259","author":"Archana","year":"2024","journal-title":"Pharmacol. 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