{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:32:43Z","timestamp":1790105563352,"version":"4.0.1"},"reference-count":69,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:00:00Z","timestamp":1773273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007061","name":"Secretar\u00eda Nacional de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["FID2024-072"],"award-info":[{"award-number":["FID2024-072"]}],"id":[{"id":"10.13039\/501100007061","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007061","name":"Secretar\u00eda Nacional de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["SNI-EGDL"],"award-info":[{"award-number":["SNI-EGDL"]}],"id":[{"id":"10.13039\/501100007061","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In this study, sixteen secondary metabolites, including two chromones, four dibenzofurans, three lipids, three depsides, two aromatic compounds, a quinone, and a terpene, were detected in the methanol:acetone (1:1 v\/v) extract of the lichen Hypotrachyna enderythraea (Zahlbr.) Hale, using High-Performance Liquid Chromatography coupled to Orbitrap Electrospray Ionization tandem Mass Spectrometry (HPLC-Orbitrap ESI tandem MS\/MS). These metabolites were characterized by analysis of their exact molecular masses and corresponding fragmentation patterns. The retention times of the identified metabolites were compared with those of standard compounds, confirming the presence of naturally occurring bioactive compounds. Density Functional Theory (DFT) calculations were employed to investigate preferential deprotonation sites in representative polyprotic metabolites. All these findings may contribute to expanding the spectrum of compounds identified within the genus Hypotrachyna and to evaluating their potential biological activities.<\/jats:p>","DOI":"10.3390\/molecules31060954","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T14:46:31Z","timestamp":1773326791000},"page":"954","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification of Secondary Metabolites from the Lichen Hypotrachyna enderythraea (Zahlbr.) Hale by HPLC-ESI-MS\/MS"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7746-8627","authenticated-orcid":false,"given":"Fernando","family":"Carrasco","sequence":"first","affiliation":[{"name":"Facultad de Ingenier\u00eda, Universidad de Lima, Av. Javier Prado Este 4600, Lima 15023, Peru"},{"name":"Departamento Acad\u00e9mico de Qu\u00edmica Org\u00e1nica, Escuela de Qu\u00edmica, Facultad de Qu\u00edmica e Ingenier\u00eda Qu\u00edmica, Universidad Nacional Mayor de San Marcos, Calle German Amezaga 375, Lima 15081, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfredo","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Facultad de Ingenier\u00eda, Universidad de Lima, Av. 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