{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T10:42:53Z","timestamp":1774348973621,"version":"3.50.1"},"reference-count":55,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001321","name":"South African National Research Foundation","doi-asserted-by":"publisher","award":["PMDS230711131848"],"award-info":[{"award-number":["PMDS230711131848"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Tshwane University of Technology research grants of JLS and CT"},{"name":"NRF Innovation Doctoral Scholarships","award":["131247"],"award-info":[{"award-number":["131247"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Agapanthus africanus (L.) Hoffmanns. is a medicinal plant traditionally used in South Africa for its promise as a source of bioactive compounds with anticancer properties. This study aimed to investigate the apoptotic effects of A. africanus fractions on cancer cell lines and to identify the bioactive phytochemical constituents using gas chromatography-mass spectrometry analysis. To test for cytotoxicity, MCF-7, A549, and HeLa cancer cells were treated with crude extract, n-hexane, n-butanol, dichloromethane, and aqueous fractions of A. africanus extracts at different concentrations (0.00\u20131000 \u00b5g\/mL). Total apoptosis was quantified using Annexin V\/PI staining. The 4\u2032,6-diamidino-2-phenylindole was used to detect nuclear morphological changes and the Caspase-GLO 3\/7 assay was employed to check the caspase activation in the cancer cells. Expression of apoptosis-related (caspase-3, bax, bcl-2) genes was evaluated using real time-polymerase chain reaction. The crude extract of A. africanus exhibited dose-dependent cytotoxicity against MCF-7, A549, and HeLa cells, with IC50 values of 130 \u00b5g\/mL, 380 \u00b5g\/mL, and &lt;125 \u00b5g\/mL, respectively. Among the tested fractions, the n-butanol fraction showed cytotoxicity towards MCF-7 cells with an IC50 value of &lt;870 \u00b5g\/mL. In contrast, n-hexane, dichloromethane and the aqueous fractions exhibited higher IC50 values against cancer cells. Flow cytometry analysis, which was applied to quantify total apoptosis, revealed that the crude extract of A. africanus induced apoptosis by (~60%) compared to the n-butanol fraction, which exhibited a moderate apoptotic effect (~27%). DAPI nuclear staining showed nuclear shrinkage and chromatin condensation in the MCF-7 cell line, whereas in Caspase-GLO 3\/7, the crude extract and n-butanol fraction resulted in significant luminescence, indicating activation of caspase-3\/7. Caspase-3\/7 analysis showed A. africanus treatments produced varying levels of apoptotic activation. The crude extract increased caspase activity by 2.9-fold, while the n-butanol fraction induced a 1.7-fold rise compared with untreated cells. GC-MS chromatograms detected and identified 16 compounds in the fractionated n-butanol and 23 compounds from the crude extract of A. africanus. The major compounds identified from the n-butanol fraction included n-hexadecanoic acid; \u03b1-tocopherol and 9,12,15-octadecatrienoic acid, while the GC\u2013MS profile of the crude extract was dominated by 6,10,14-trimethylpentadecan-2-one; 1,3,5-Triphenylcyclohexane and phytol. The study indicates the pro-apoptotic potential of A. africanus, particularly in its crude form, supporting its ethnopharmacological use and suggesting its relevance as a candidate for anticancer drug discovery.<\/jats:p>","DOI":"10.3390\/molecules31071062","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T08:02:49Z","timestamp":1774339369000},"page":"1062","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Apoptotic Effects of Agapanthus africanus Extracts and Identification of Volatile Compounds from the n-Butanol Fraction"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4965-9343","authenticated-orcid":false,"given":"Makgwale S.","family":"Mphahlele","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8687-001X","authenticated-orcid":false,"given":"Kingsley C.","family":"Mbara","sequence":"additional","affiliation":[{"name":"Nanomedicines Manufacturing, Biopharmaceutics and Diagnostics Research Laboratory, Department of Pharmaceutical Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9960-5565","authenticated-orcid":false,"given":"Daniel M.","family":"Tswaledi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, School of Science and Technology, Sefako Makgatho Health Sciences University, Pretoria 0204, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8394-7096","authenticated-orcid":false,"given":"Raymond T.","family":"Makola","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Biotechnology, School of Molecular and Life Science, University of Limpopo, Turflop Campus, Sovenga 0727, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5444-4457","authenticated-orcid":false,"given":"Clemence","family":"Tarirai","sequence":"additional","affiliation":[{"name":"Nanomedicines Manufacturing, Biopharmaceutics and Diagnostics Research Laboratory, Department of Pharmaceutical Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4799-7437","authenticated-orcid":false,"given":"Jeremia L.","family":"Shai","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/S1470-2045(23)00182-1","article-title":"Global funding for cancer research between 2016 and 2020: A content analysis of public and philanthropic investments","volume":"24","author":"McIntosh","year":"2023","journal-title":"Lancet Oncol."},{"key":"ref_2","first-page":"209","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. Clin."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"220","DOI":"10.4102\/sajo.v6i0.220","article-title":"Estimating the burden of cancer in South Africa","volume":"6","author":"Finestone","year":"2022","journal-title":"S. Afr. J. Oncol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e414","DOI":"10.1016\/S1470-2045(15)00162-X","article-title":"Establishment of a cancer surveillance programme: The South African experience","volume":"16","author":"Singh","year":"2015","journal-title":"Lancet Oncol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"117535","DOI":"10.1016\/j.ejmech.2025.117535","article-title":"Mechanistic study of cancer drug delivery: Current techniques, limitations, and prospects","volume":"290","author":"Imtiaz","year":"2025","journal-title":"Eur. J. Med. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"100681","DOI":"10.1016\/j.phyplu.2024.100681","article-title":"Evaluation of Southern African wild edible plants for potential herb-drug interactions through ex vivo p-glycoprotein and in vitro cytochrome P450 3A4 inhibitory effects","volume":"5","author":"Kheoane","year":"2025","journal-title":"Phytomed. Plus"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s00606-003-0038-6","article-title":"Taxonomic implications of genome size and pollen colour and vitality for species of Agapanthus L\u2019H\u00e9ritier (Agapanthaceae)","volume":"241","author":"Zonneveld","year":"2003","journal-title":"Plant Syst. Evol."},{"key":"ref_8","unstructured":"Manning, J., and Goldblatt, P. (2012). Plants of the Greater Cape Floristic Region. 1: The Core Cape Flora, CABI."},{"key":"ref_9","unstructured":"Rebelo, A.G., Boucher, C., Helme, N., Mucina, L., and Rutherford, M.C. (2006). Fynbos biome 4. The Vegetation of South Africa, Lesotho and Swaziland, SANBI."},{"key":"ref_10","unstructured":"Pooley, E. (1998). A Field Guide to Wildflowers: KwaZulu-Natal and the Eastern Region, The Flora & Fauna Publications Trust."},{"key":"ref_11","unstructured":"Hutchings, A., and Scott, A.H. (1996). Zulu Medicinal Plants: An Inventory, University of Natal Press."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0378-8741(98)00224-4","article-title":"Pharmacological effects of Agapanthus africanus on the isolated rat uterus","volume":"66","author":"Veale","year":"1999","journal-title":"J. Ethnopharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1016\/j.sajb.2022.09.029","article-title":"The genus Agapanthus: A review of traditional uses, pharmacological and phytochemical attributes","volume":"150","author":"Younis","year":"2022","journal-title":"S. Afr. J. Bot."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2808","DOI":"10.1021\/acsomega.2c07766","article-title":"Structure Elucidation of 16 Undescribed Steroidal Glycosides from the Underground Parts of Agapanthus africanus and Apoptosis-Inducing Activity in Small-Cell Lung Cancer Cells","volume":"8","author":"Takahashi","year":"2023","journal-title":"ACS Omega"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Siddik, Z.H., and Mehta, K. (2009). Oxidative Stress and Drug Resistance in Cancer. Drug Resistance in Cancer Cells, Springer.","DOI":"10.1007\/978-0-387-89445-4"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1186\/s12937-016-0217-2","article-title":"An overview on the role of dietary phenolics for the treatment of cancers","volume":"15","author":"Anantharaju","year":"2016","journal-title":"Nutr. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1080\/01926230701320337","article-title":"Apoptosis: A Review of Programmed Cell Death","volume":"35","author":"Elmore","year":"2007","journal-title":"Toxicol. Pathol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1146\/annurev-genet-102108-134850","article-title":"The Role of Mitochondria in Apoptosis","volume":"43","author":"Wang","year":"2009","journal-title":"Annu. Rev. Genet."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.sajb.2024.05.026","article-title":"Phytochemical characterisation and biological evaluation of Agapanthus africanus: A combined experimental and in silico study","volume":"170","author":"Gomaa","year":"2024","journal-title":"S. Afr. J. Bot."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Iguchi, T., Shimazaki, T., and Mimaki, Y. (2025). Agapanthussaponin A from the Underground Parts of Agapanthus africanus Induces Apoptosis and Ferroptosis in Human Small-Cell Lung Cancer Cells. Molecules, 30.","DOI":"10.3390\/molecules30153189"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e40036","DOI":"10.14393\/BJ-v40n0a2024-71135","article-title":"Dodecanoic acid induces oxidative stress-mediated death in liver cancer cells through the mitochondrial pathway","volume":"40","author":"Chen","year":"2024","journal-title":"Biosci. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.6026\/973206300191075","article-title":"Anti-inflammatory activity of lauric acid, thiocolchicoside and thiocolchicoside-lauric acid formulation","volume":"19","author":"Mustafa","year":"2023","journal-title":"Bioinformation"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"130229","DOI":"10.1016\/j.molstruc.2021.130229","article-title":"Evaluation of the anticancer potential of Hexadecanoic acid from brown algae Turbinaria ornata on HT\u201329 colon cancer cells","volume":"1235","author":"Bharath","year":"2021","journal-title":"J. Mol. Struct."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Mathe, E., Sethoga, L., Mapfumari, S., Adeniran, O., Mokgotho, P., Shai, J., and Gololo, S. (2024). Phytochemical Screening and Characterisation of Volatile Compounds from Three Medicinal Plants with Reported Anticancer Properties Using GC-MS. Life, 14.","DOI":"10.3390\/life14111375"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"105853","DOI":"10.1016\/j.fbio.2025.105853","article-title":"Exploring the anti-colon cancer potential of Polygonum minus: Integrating in vitro and in silico studies","volume":"64","author":"Yang","year":"2025","journal-title":"Food Biosci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Park, J.E., Lee, T.H., Ham, S.L., Subedi, L., Hong, S.M., Kim, S.Y., Choi, S.U., Kim, C.S., and Lee, K.R. (2022). Anticancer and Anti-Neuroinflammatory Constituents Isolated from the Roots of Wasabia japonica. Antioxidants, 11.","DOI":"10.3390\/antiox11030482"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1080\/14786419.2014.921686","article-title":"An insight into the cytotoxic activity of phytol at in vitro conditions","volume":"28","author":"Pejin","year":"2014","journal-title":"Nat. Prod. Res."},{"key":"ref_28","first-page":"1","article-title":"Phytol induces apoptosis and ROS-mediated protective autophagy in human gastric adenocarcinoma AGS cells","volume":"4","author":"Song","year":"2015","journal-title":"Biochem. Anal. Biochem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0165-0270(98)00147-2","article-title":"Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells","volume":"86","author":"Schutte","year":"1998","journal-title":"J. Neurosci. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Neffati, M., Najjaa, H., and M\u00e1th\u00e9, \u00c1. (2017). A Review of African Medicinal and Aromatic Plants. Medicinal and Aromatic Plants of the World\u2014Africa Volume 3, Springer.","DOI":"10.1007\/978-94-024-1120-1"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.jep.2006.04.003","article-title":"Anti-infective potential of natural products: How to develop a stronger in vitro \u2018proof-of-concept\u2019","volume":"106","author":"Cos","year":"2006","journal-title":"J. Ethnopharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.phymed.2008.12.018","article-title":"Synergy research: Approaching a new generation of phytopharmaceuticals","volume":"16","author":"Wagner","year":"2009","journal-title":"Phytomedicine"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Chaudhry, G.-e.-S., Md Akim, A., Sung, Y.Y., and Sifzizul, T.M.T. (2022). Cancer and apoptosis: The apoptotic activity of plant and marine natural products and their potential as targeted cancer therapeutics. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.842376"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1007\/s10495-016-1333-3","article-title":"Methods to detect apoptotic cell death","volume":"22","author":"Banfalvi","year":"2017","journal-title":"Apoptosis"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5389","DOI":"10.1007\/s00216-014-7949-7","article-title":"A miniaturised device for bioluminescence analysis of caspase-3\/7 activity in a single apoptotic cell","volume":"406","year":"2014","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/sj.cdd.4400476","article-title":"Emerging roles of caspase-3 in apoptosis","volume":"6","author":"Porter","year":"1999","journal-title":"Cell Death Differ."},{"key":"ref_37","first-page":"69","article-title":"The value of plants used in traditional medicine for drug discovery","volume":"109","author":"Fabricant","year":"2001","journal-title":"Environ. Health Perspect."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1152\/physiolgenomics.00025.2005","article-title":"GAPDH as a housekeeping gene: Analysis of GAPDH mRNA expression in a panel of 72 human tissues","volume":"21","author":"Barber","year":"2005","journal-title":"Physiol. Genom."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1152\/physrev.00013.2006","article-title":"Mitochondrial Membrane Permeabilisation in Cell Death","volume":"87","author":"Kroemer","year":"2007","journal-title":"Physiol. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.cell.2011.02.013","article-title":"Hallmarks of Cancer: The Next Generation","volume":"144","author":"Hanahan","year":"2011","journal-title":"Cell"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.jep.2009.09.014","article-title":"Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts","volume":"126","author":"Fronza","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"132","DOI":"10.4103\/2221-1691.338922","article-title":"Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress","volume":"12","author":"Sangpairoj","year":"2022","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"448","DOI":"10.4103\/apjtb.apjtb_395_24","article-title":"Phytochemical profiling and anticancer potential of Cymbopogon citratus extract","volume":"14","author":"Almutairi","year":"2024","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Fan, H., Huang, W., Guo, Y., Ma, X., and Yang, J. (2022). \u03b1-Linolenic Acid Suppresses Proliferation and Invasion in Osteosarcoma Cells via Inhibiting Fatty Acid Synthase. Molecules, 27.","DOI":"10.3390\/molecules27092741"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Wadoo, R., Ali, T., Jan, I., Mir, S.A., Amin, A., Qadri, R., Alshehri, S., Shakeel, F., Bader, G.N., and Wani, S.U.D. (2025). In-vitro antioxidant and anti-proliferative activity of aerial parts of Senecio Laetus Edgew on breast cancer (MCF-7) and colon carcinoma (HCT116) cell lines. BMC Complement. Med. Ther., 25.","DOI":"10.1186\/s12906-025-04789-y"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Moore, C., Palau, V.E., Mahboob, R., Lightner, J., Stone, W., and Krishnan, K. (2020). Upregulation of pERK and c-JUN by \u03b3-tocotrienol and not \u03b1-tocopherol are essential to the differential effect on apoptosis in prostate cancer cells. BMC Cancer, 20.","DOI":"10.1186\/s12885-020-06947-6"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Sharifi-Rad, J., Sureda, A., Tenore, G.C., Daglia, M., Sharifi-Rad, M., Valussi, M., Tundis, R., Sharifi-Rad, M., Loizzo, M.R., and Ademiluyi, A.O. (2017). Biological Activities of Essential Oils: From Plant Chemoecology to Traditional Healing Systems. Molecules, 22.","DOI":"10.3390\/molecules22010070"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Isa, A.I., Awouafack, M.D., Dzoyem, J.P., Aliyu, M., Magaji, R.A., Ayo, J.O., and Eloff, J.N. (2014). Some Strychnos spinosa (Loganiaceae) leaf extracts and fractions have good antimicrobial activities and low cytotoxicities. BMC Complement. Altern. Med., 14.","DOI":"10.1186\/1472-6882-14-456"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0378-8741(99)00161-0","article-title":"Cytotoxicity of some medicinal plant extracts used in Tanzanian traditional medicine","volume":"70","author":"Kamuhabwa","year":"2000","journal-title":"J. Ethnopharmacol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colourimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.brainresprot.2004.04.001","article-title":"Quantification of sPLA2-induced early and late apoptosis changes in neuronal cell cultures using combined TUNEL and DAPI staining","volume":"13","author":"Daniel","year":"2004","journal-title":"Brain Res. Protoc."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/s00216-016-9998-6","article-title":"Parallel single-cell analysis of active caspase-3\/7 in apoptotic and non-apoptotic cells","volume":"409","author":"Ledvina","year":"2017","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/1476-4598-7-50","article-title":"Effect of bortezomib on human neuroblastoma: Analysis of molecular mechanisms involved in cytotoxicity","volume":"7","author":"Combaret","year":"2008","journal-title":"Mol. Cancer"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"115542","DOI":"10.1016\/j.jep.2022.115542","article-title":"Antidiabetic effects of Psidium x durbanensis Baijnath & Ramcharun ined. (Myrtaceae)Leaf extract on streptozotocin-induced diabetes in rats","volume":"297","author":"Mbara","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1186\/s13287-023-03488-0","article-title":"Paracrine signalling between keratinocytes and SVF cells results in a new secreted cytokine profile during wound closure","volume":"14","author":"Balko","year":"2023","journal-title":"Stem Cell Res. Ther."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/7\/1062\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T08:03:33Z","timestamp":1774339413000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/7\/1062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,24]]},"references-count":55,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["molecules31071062"],"URL":"https:\/\/doi.org\/10.3390\/molecules31071062","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,24]]}}}