{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:22:55Z","timestamp":1785435775999,"version":"3.56.0"},"reference-count":37,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS-PAN Joint Research Project"},{"DOI":"10.13039\/501100007533","name":"Kobayashi Foundation for Cancer Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007533","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/02qjq5342","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008656","name":"Iketani Science and Technology Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008656","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/0382jhd52","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019433","name":"Shibuya Science Culture and Sports Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100019433","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>[111In]In-diethylenetriaminepentaacetic acid-5,10,15,20-tetraphenylporphyrin ([111In]In-DTPA-TPP) nanodroplets were developed for cancer theranostics, featuring ultrasound-sensitive properties. The designed nanodroplets that encapsulate the low-boiling-point liquid perfluorocarbon and IR-780 iodide, a near-infrared fluorescent dye, with surface conjugation of 111In-labeled porphyrin derivative, were synthesized and evaluated by in vitro and in vivo experiments. The cellular uptake of [111In]In-DTPA-TPP nanodroplets was significantly higher than that of control nanodroplets without TPP. Biodistribution experiments revealed greater tumor accumulation in mice injected with [111In]In-DTPA-TPP nanodroplets than in those injected with control nanodroplets lacking TPP. Additionally, the accumulation of [111In]In-DTPA-TPP nanodroplets in the tumor was visualized by single-photon emission computed tomography. Sonodynamic therapeutic experiments revealed that DTPA-TPP nanodroplets at 10 \u00b5mol total lipids\/kg weight with a single ultrasound irradiation onto the tumor area significantly inhibited tumor growth. These results indicate that [111In]In-DTPA-TPP nanodroplets would be promising cancer theranostic agents.<\/jats:p>","DOI":"10.3390\/molecules31071114","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T09:57:24Z","timestamp":1774864644000},"page":"1114","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preparation and Evaluation of Radiolabeled Porphyrin-Functionalized Lipid Nanodroplets for Cancer Theranostics"],"prefix":"10.3390","volume":"31","author":[{"given":"Nur Izni Binti","family":"Ramzi","sequence":"first","affiliation":[{"name":"Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kisa","family":"Tamamura","sequence":"additional","affiliation":[{"name":"Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3405-4512","authenticated-orcid":false,"given":"Masayuki","family":"Munekane","sequence":"additional","affiliation":[{"name":"Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5071-7574","authenticated-orcid":false,"given":"Kenji","family":"Mishiro","sequence":"additional","affiliation":[{"name":"Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8141-1212","authenticated-orcid":false,"given":"Takeshi","family":"Fuchigami","sequence":"additional","affiliation":[{"name":"Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Hu","sequence":"additional","affiliation":[{"name":"Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7989-5083","authenticated-orcid":false,"given":"Renata","family":"Jastrz\u0105b","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Adama Mickiewicz University in Poznan, Uniwersytetu Pozna\u0144skiego 8, 61-614 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seigo","family":"Kinuya","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, Kanazawa University Hospital, Kanazawa University, Takara-machi 13-1, Kanazawa 920-8641, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuaki","family":"Ninomiya","sequence":"additional","affiliation":[{"name":"Faculty of Biological Science and Technology, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1691-7302","authenticated-orcid":false,"given":"Kazuma","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Ishikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Gu, Z., Da Silva, C.G., Van der Maaden, K., Ossendorp, F., and Cruz, L.J. (2020). Liposome-Based Drug Delivery Systems in Cancer Immunotherapy. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12111054"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3379","DOI":"10.2147\/IJN.S504503","article-title":"Extensive Review of Nanomedicine Strategies Targeting the Tumor Microenvironment in PDAC","volume":"20","author":"Liu","year":"2025","journal-title":"Int. J. Nanomed."},{"key":"ref_3","first-page":"742","article-title":"Progress in Nanomedicine: Approved and Investigational Nanodrugs","volume":"42","author":"Ventola","year":"2017","journal-title":"Pharm. Ther."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Elghobary, M.E.N., Munekane, M., Mishiro, K., Fuchigami, T., and Ogawa, K. (2023). Preparation and Evaluation of Thermosensitive Liposomes Encapsulating I-125-Labeled Doxorubicin Derivatives for Auger Electron Therapy. Molecules, 28.","DOI":"10.3390\/molecules28041864"},{"key":"ref_5","first-page":"6387","article-title":"A new concept for macromolecular therapeutics in cancer chemotherapy: Mechanism of tumoritropic accumulation of proteins and the antitumor agent smancs","volume":"46","author":"Matsumura","year":"1986","journal-title":"Cancer Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"8018","DOI":"10.7150\/thno.38587","article-title":"Transcytosis\u2014An effective targeting strategy that is complementary to \u201cEPR effect\u201d for pancreatic cancer nano drug delivery","volume":"9","author":"Liu","year":"2019","journal-title":"Theranostics"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1016\/j.drudis.2006.07.005","article-title":"Exploiting the enhanced permeability and retention effect for tumor targeting","volume":"11","author":"Iyer","year":"2006","journal-title":"Drug Discov. Today"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jconrel.2011.09.063","article-title":"Drug targeting to tumors: Principles, pitfalls and (pre-) clinical progress","volume":"161","author":"Lammers","year":"2012","journal-title":"J. Control Release"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4385","DOI":"10.1021\/nn406258m","article-title":"Radioactive 198Au-Doped Nanostructures with Different Shapes for In Vivo Analyses of Their Biodistribution, Tumor Uptake, and Intratumoral Distribution","volume":"8","author":"Black","year":"2014","journal-title":"ACS Nano"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.jconrel.2016.11.015","article-title":"To exploit the tumor microenvironment: Since the EPR effect fails in the clinic, what is the future of nanomedicine?","volume":"244","author":"Danhier","year":"2016","journal-title":"J. Control Release"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2225","DOI":"10.1021\/acs.bioconjchem.6b00437","article-title":"Nanodrug Delivery: Is the Enhanced Permeability and Retention Effect Sufficient for Curing Cancer?","volume":"27","author":"Nakamura","year":"2016","journal-title":"Bioconjug. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1038\/nmat3792","article-title":"Strategies for advancing cancer nanomedicine","volume":"12","author":"Chauhan","year":"2013","journal-title":"Nat. Mater."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5728","DOI":"10.1021\/acs.molpharmaceut.4c00732","article-title":"Development of Radiolabeled Probes with Improved Imaging Contrast by Releasing Urinary Excretable Radiolabeled Compounds from Thermosensitive Liposomes in the Blood","volume":"21","author":"Munekane","year":"2024","journal-title":"Mol. Pharm."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0304-3835(95)03762-L","article-title":"Tumor-localizing activity of porphyrin and its affinity to LDL, transferrin","volume":"92","author":"Nakajima","year":"1995","journal-title":"Cancer Lett."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1093\/oxfordjournals.jbchem.a122396","article-title":"Mechanisms involved in the cellular uptake of hematoporphyrin by rat hepatoma cells","volume":"103","author":"Naitoh","year":"1988","journal-title":"J. Biochem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/S1357-2725(01)00092-9","article-title":"Low-density lipoprotein receptors in the uptake of tumour photosensitizers by human and rat transformed fibroblasts","volume":"34","author":"Polo","year":"2002","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1142\/S1088424612501192","article-title":"Cancer cells uptake porphyrins via heme carrier protein 1","volume":"17","author":"Hiyama","year":"2013","journal-title":"J. Porphyr. Phthalocyanines"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1002\/jlcr.3166","article-title":"Radiolabelled porphyrins in nuclear medicine","volume":"57","author":"Waghorn","year":"2014","journal-title":"J. Labelled Comp. Radiopharm."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Austin, E., Wang, J.Y., Ozog, D.M., Zeitouni, N., Lim, H.W., and Jagdeo, J. (2025). Photodynamic Therapy: Overview and Mechanism of Action. J. Am. Acad. Dermatol.","DOI":"10.1016\/j.jaad.2025.02.037"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s00424-020-02482-2","article-title":"Perfluorocarbon-based oxygen carriers: From physics to physiology","volume":"473","author":"Jagers","year":"2021","journal-title":"Pflugers Arch."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3023","DOI":"10.1039\/D1MA01197A","article-title":"Making waves: How ultrasound-targeted drug delivery is changing pharmaceutical approaches","volume":"3","author":"Delaney","year":"2022","journal-title":"Mater. Adv."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"8785","DOI":"10.1038\/ncomms9785","article-title":"Perfluorocarbon nanoparticles enhance reactive oxygen levels and tumour growth inhibition in photodynamic therapy","volume":"6","author":"Cheng","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"81591","DOI":"10.18632\/oncotarget.20189","article-title":"Porphyrin photosensitizers in photodynamic therapy and its applications","volume":"8","author":"Kou","year":"2017","journal-title":"Oncotarget"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.freeradbiomed.2012.04.025","article-title":"Tumor growth inhibition by sonodynamic therapy using a novel sonosensitizer","volume":"53","author":"Tsuru","year":"2012","journal-title":"Free Radic. Biol. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"27934","DOI":"10.1021\/acsami.1c06381","article-title":"Water-Soluble Iridic-Porphyrin Complex for Non-invasive Sonodynamic and Sono-oxidation Therapy of Deep Tumors","volume":"13","author":"Xie","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2956","DOI":"10.1021\/acsbiomaterials.0c00407","article-title":"Perfluorohexane-Loaded Polymeric Nanovesicles with Oxygen Supply for Enhanced Sonodynamic Therapy","volume":"6","author":"Zeng","year":"2020","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0014-5793(90)81016-H","article-title":"Amphipathic polyethyleneglycols effectively prolong the circulation time of liposomes","volume":"268","author":"Klibanov","year":"1990","journal-title":"FEBS Lett."},{"key":"ref_28","first-page":"3673","article-title":"Sonodynamic therapy of cancer using novel sonosensitizers","volume":"27","author":"Kuroki","year":"2007","journal-title":"Anticancer Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1186\/s12967-016-1033-3","article-title":"Liposome encapsulated perfluorohexane enhances radiotherapy in mice without additional oxygen supply","volume":"14","author":"Xu","year":"2016","journal-title":"J. Transl. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"25968","DOI":"10.1038\/srep25968","article-title":"IR-780 Dye as a Sonosensitizer for Sonodynamic Therapy of Breast Tumor","volume":"6","author":"Li","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4218","DOI":"10.1039\/C6CS00636A","article-title":"Cellular uptake of nanoparticles: Journey inside the cell","volume":"46","author":"Behzadi","year":"2017","journal-title":"Chem. Soc. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1039\/D2MD00234E","article-title":"Synthesis and evaluation of radiolabeled porphyrin derivatives for cancer diagnoses and their nonradioactive counterparts for photodynamic therapy","volume":"13","author":"Ramzi","year":"2022","journal-title":"RSC Med. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4190","DOI":"10.1158\/1078-0432.CCR-16-3193","article-title":"64Cu-MM-302 Positron Emission Tomography Quantifies Variability of Enhanced Permeability and Retention of Nanoparticles in Relation to Treatment Response in Patients with Metastatic Breast Cancer","volume":"23","author":"Lee","year":"2017","journal-title":"Clin. Cancer Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1038\/bjc.1990.403","article-title":"Tumour scanning with indium-111 dihaematoporphyrin ether","volume":"62","author":"Quastel","year":"1990","journal-title":"Br. J. Cancer"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.ultsonch.2015.06.032","article-title":"Targeted and ultrasound-triggered cancer cell injury using perfluorocarbon emulsion-loaded liposomes endowed with cancer cell-targeting and fusogenic capabilities","volume":"28","author":"Ninomiya","year":"2016","journal-title":"Ultrason. Sonochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.nucmedbio.2013.02.008","article-title":"Development and evaluation of a radiobromine-labeled sigma ligand for tumor imaging","volume":"40","author":"Ogawa","year":"2013","journal-title":"Nucl. Med. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1246\/bcsj.20180382","article-title":"A Platinum Functional Porphyrin Conjugate: An Excellent Cancer Killer for Photodynamic Therapy","volume":"92","author":"Hu","year":"2019","journal-title":"Bull. Chem. Soc. Jpn."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/7\/1114\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T15:48:46Z","timestamp":1775058526000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/7\/1114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,27]]},"references-count":37,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["molecules31071114"],"URL":"https:\/\/doi.org\/10.3390\/molecules31071114","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,27]]}}}