{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:41:46Z","timestamp":1790217706784,"version":"4.1.0"},"reference-count":70,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T00:00:00Z","timestamp":1774742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Leishmaniasis is caused by parasitic protozoans of the Leishmania genus and has been classified as a Neglected Tropical Disease. Control of this parasite relies mainly on chemotherapy; however, conventional available drugs are unsatisfactory. Phytomedicine, particularly essential oils, is a promising alternative. In this study, the chemical composition and antileishmanial properties of essential oil from leaves of Schinus terebinthifolia Raddi (EO-St) were determined. Chemical components were identified by GC-MS. Antileishmanial activity on promastigotes of L. amazonensis was assayed, followed by the evaluation of the essential oil\u2019s effects on the mitochondrial membrane potential and redox state of the parasite. Finally, the activity was confirmed on intracellular amastigotes and compared with cytotoxicity on peritoneal macrophages from BALB\/c mice. In the essential oil, 78 compounds were identified. The major component was \u03b4-3-carene with 14.8%. The IC50 values of 18.2 \u00b1 1.4 \u00b5g\/mL and 15.0 \u00b1 1.6 \u00b5g\/mL against promastigote and amastigote forms, respectively, were obtained. The cytotoxicity for the host cells was approximately four-fold higher than those for the parasite. The essential oil was able to cause a disruption in the mitochondrial membrane potential. The quantified redox parameters showed statistical differences (p &lt; 0.05) between EO-St-treated cultures and control groups (untreated and treated with DMSO). In summary, EO-St was active in vitro against both forms of L. amazonensis, possible mediated by mitochondrial dysfunction and redox imbalance.<\/jats:p>","DOI":"10.3390\/molecules31071125","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T09:57:24Z","timestamp":1774864644000},"page":"1125","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antileishmanial Activity of Schinus terebinthifolia Essential Oil: Chemistry, In Vitro and Mechanistic Studies"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1958-809X","authenticated-orcid":false,"given":"Lianet","family":"Monzote","sequence":"first","affiliation":[{"name":"Parasitology Department, Institute of Tropical Medicine \u201cPedro Kouri\u201d, Havana 10400, Cuba"},{"name":"Research Network Natural Products Against Neglected Diseases (ResNetNPND), 48149 Munster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lillyam","family":"Betancourt","sequence":"additional","affiliation":[{"name":"Pharmacological Research Department, Institute of Tropical Medicine \u201cPedro Kouri\u201d, Havana 10400, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n","family":"Scull","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Institute of Pharmacy and Food, Havana University, La Coronela, La Lisa, Havana 13600, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2950-1074","authenticated-orcid":false,"given":"Prabodh","family":"Satyal","sequence":"additional","affiliation":[{"name":"Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizette","family":"Gil","sequence":"additional","affiliation":[{"name":"Pharmacological Research Department, Institute of Tropical Medicine \u201cPedro Kouri\u201d, Havana 10400, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1223-0201","authenticated-orcid":false,"given":"Lars","family":"Gille","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, Department of Biomedical Sciences, University of Veterinary Medicine, Veterin\u00e4rplatz 1, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3639-0528","authenticated-orcid":false,"given":"William N.","family":"Setzer","sequence":"additional","affiliation":[{"name":"Research Network Natural Products Against Neglected Diseases (ResNetNPND), 48149 Munster, Germany"},{"name":"Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA"},{"name":"Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Selvapandiyan, A., Croft, S.L., Rijal, S., Nakhasi, H.L., and Ganguly, N.K. (2019). Innovations for the Elimination and Control of Visceral Leishmaniasis. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007616"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"308","DOI":"10.2174\/2589977515666230725105141","article-title":"The Prospects of Phytomedicines and Nanomedicines to Treat Leishmaniasis: A Comprehensive Review","volume":"16","author":"Swami","year":"2024","journal-title":"Curr. Drug Res. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e27","DOI":"10.1017\/erm.2025.4","article-title":"An Update on Clinical and Pathogenic Spectra of Leishmaniasis","volume":"27","author":"Selvapandiyan","year":"2025","journal-title":"Expert Rev. Mol. Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ol\u00edas-Molero, A.I., de la Fuente, C., Cuquerella, M., Torrado, J.J., and Alunda, J.M. (2021). Antileishmanial Drug Discovery and Development: Time to Reset the Model?. Microorganisms, 9.","DOI":"10.3390\/microorganisms9122500"},{"key":"ref_5","first-page":"61","article-title":"Field-Deployable Treatments for Leishmaniasis: Intrinsic Challenges, Recent Developments and next Steps","volume":"14","author":"Markle","year":"2023","journal-title":"Res. Rep. Trop. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"6587150","DOI":"10.1155\/2019\/6587150","article-title":"Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses","volume":"2019","author":"Luna","year":"2019","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1017\/S0031182020001304","article-title":"Effect of Essential Oils on Leishmania amazonensis: A Systematic Review","volume":"147","author":"Oyama","year":"2020","journal-title":"Parasitology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1002\/1099-1573(200011)14:7<549::AID-PTR623>3.0.CO;2-T","article-title":"Myorelaxant and Antispasmodic Effects of the Essential Oil of Alpinia speciosa on Rat Ileum","volume":"14","author":"Bezerra","year":"2000","journal-title":"Phyther. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.pt.2005.08.004","article-title":"Plant Active Components\u2014A Resource for Antiparasitic Agents?","volume":"21","author":"Anthony","year":"2005","journal-title":"Trends Parasitol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4833","DOI":"10.1080\/14786419.2021.2012772","article-title":"The Genus Schinus (Anacardiaceae): A Review on Phytochemicals and Biological Aspects","volume":"36","author":"Mostafa","year":"2022","journal-title":"Nat. Prod. Res."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Abbas, M.G., Azeem, M., Bashir, M.U., Ali, F., Moz\u016bratis, R., and Binyameen, M. (2024). Chemical Composition, Repellent, and Oviposition Deterrent Potential of Wild Plant Essential Oils against Three Mosquito Species. Molecules, 29.","DOI":"10.3390\/molecules29112657"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5265","DOI":"10.1021\/jf900638c","article-title":"Chemical Compositions and Antioxidant\/Antimicrobial Activities of Various Samples Prepared from Schinus terebinthifolius Leaves Cultivated in Egypt","volume":"57","author":"Shaaban","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Oliveira, K.C., Franciscato, L.M.S.S., Mendes, S.S., Barizon, F.M.A., Gon\u00e7alves, D.D., Barbosa, L.N., Faria, M.G.I., Valle, J.S., Casalvara, R.F.A., and Gon\u00e7alves, J.E. (2024). Essential Oil from the Leaves, Fruits and Twigs of Schinus terebinthifolius: Chemical Composition, Antioxidant and Antibacterial Potential. Molecules, 29.","DOI":"10.3390\/molecules29020469"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.3109\/13880209.2012.666880","article-title":"Essential Oils from Schinus terebinthifolius Leaves\u2014Chemical Composition and In Vitro Cytotoxicity Evaluation","volume":"50","author":"Santana","year":"2012","journal-title":"Pharm. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Guimar\u00e3es, B.d.A., Silva, R.C., Andrade, E.H.d.A., Setzer, W.N., da Silva, J.K., and Figueiredo, P.L.B. (2023). Seasonality, Composition, and Antioxidant Capacity of Limonene\/\u03b4-3-Carene\/(E)-Caryophyllene Schinus terebinthifolia Essential Oil Chemotype from the Brazilian Amazon: A Chemometric Approach. Plants, 12.","DOI":"10.3390\/plants12132497"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ba\u015fer, K.H.C., and Buchbauer, G. (2010). Handbook of Essential Oils: Science, Technology, and Applications, CRC Press.","DOI":"10.1201\/9781420063165"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"127724","DOI":"10.1016\/j.foodchem.2020.127724","article-title":"Antioxidant and Enzyme-Inhibitory Activity of Peppermint Extracts and Essential Oils Obtained by Conventional and Emerging Extraction Techniques","volume":"338","author":"Zengin","year":"2021","journal-title":"Food Chem."},{"key":"ref_18","first-page":"1799","article-title":"Chemistry and Leishmanicidal Activity of the Essential Oil from Artemisia absinthium from Cuba","volume":"9","author":"Monzote","year":"2014","journal-title":"Nat. Prod. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e20200111","DOI":"10.1590\/1678-4324-2020200111","article-title":"Bioactivity of Schinus molle L. and Schinus terebinthifolia Raddi. Essential Oils on Anticarsia gemmatalis (H\u00fcbner 1818)","volume":"63","author":"Silvestre","year":"2020","journal-title":"Braz. Arch. Biol. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"115786","DOI":"10.1016\/j.jep.2022.115786","article-title":"Geographical Variation in the Chemical Composition, Anti-Inflammatory Activity of the Essential Oil, Micromorphology and Histochemistry of Schinus terebinthifolia Raddi","volume":"301","author":"Marangoni","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1089\/jmf.2015.0093","article-title":"An In Vitro Attempt for Controlling Severe Phytopathogens and Human Pathogens Using Essential Oils from Mediterranean Plants of Genus Schinus","volume":"19","author":"Elshafie","year":"2016","journal-title":"J. Med. Food"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1590\/S0100-40422007000800030","article-title":"Seasonal Variation in the Composition of Volatile Oils from Schinus terebinthifolius Raddi","volume":"30","author":"Barbosa","year":"2007","journal-title":"Quim. Nova"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1590\/S1517-83822010000100023","article-title":"Antibacterial Activity, Chemical Composition, and Cytotoxicity of Leaf\u2019s Essential Oil from Brazilian Pepper Tree (Schinus terebinthifolius, Raddi)","volume":"41","author":"Silva","year":"2010","journal-title":"Braz. J. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e20180895","DOI":"10.1590\/0001-3765202020180895","article-title":"Insecticidal Properties and Chemical Composition of Piper aduncum L., Lippia sidoides Cham. and Schinus terebinthifolius Raddi Essential Oils against Plutella xylostella L.","volume":"92","author":"Born","year":"2020","journal-title":"An. Acad. Bras. Cienc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1590\/S1516-05722013000500017","article-title":"Composi\u00e7\u00e3o Qu\u00edmica e Atividade Inseticida Do \u00d3leo Essencial de Schinus terebinthifolius Raddi (Anacardiaceae) Sobre a Broca-Do-Caf\u00e9 (Hypothenemus hampei) Ferrari","volume":"15","author":"Santos","year":"2013","journal-title":"Rev. Bras. Plantas Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1080\/0972060X.2009.10643686","article-title":"Chemical Composition of the Essential Oils from Leaves and Fruits of Schinus molle L. and Schinus terebinthifolius Raddi from Southern Brazil","volume":"12","author":"Rossato","year":"2009","journal-title":"J. Essent. Oil Bear. Plants"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"102260","DOI":"10.1016\/j.jspr.2024.102260","article-title":"Potential Agrochemical Applications of Schinus terebinthifolius Essential Oil","volume":"105","author":"Bernardi","year":"2024","journal-title":"J. Stored Prod. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.indcrop.2015.11.077","article-title":"Composition and Biological Activity of Brazilian Rose Pepper (Schinus terebinthifolius Raddi) Leaves","volume":"83","author":"Uliana","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1007\/s13197-018-3049-6","article-title":"Assessing the Fatty Acid, Essential Oil Composition, Their Radical Scavenging and Antibacterial Activities of Schinus terebinthifolius Raddi Leaves and Twigs","volume":"55","author":"Ennigrou","year":"2018","journal-title":"J. Food Sci. Technol."},{"key":"ref_30","unstructured":"World Flora Online (2026, March 18). An Online Flora of All Known Plants. Available online: https:\/\/www.worldfloraonline.org\/."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1023\/A:1013386921596","article-title":"Regulation of Essential Oil Production in Plants","volume":"34","author":"Sangwan","year":"2001","journal-title":"Plant Growth Regul."},{"key":"ref_32","first-page":"1147","article-title":"Factors Affecting Chemical Variability of Essential Oils: A Review of Recent Developments","volume":"4","author":"Barra","year":"2009","journal-title":"Nat. Prod. Commun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1111\/nph.12526","article-title":"Explaining Intraspecific Diversity in Plant Secondary Metabolites in an Ecological Context","volume":"201","author":"Moore","year":"2014","journal-title":"New Phytol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Etri, K., and Pluh\u00e1r, Z. (2024). Exploring Chemical Variability in the Essential Oils of the Thymus Genus. Plants, 4.","DOI":"10.3390\/plants13101375"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Hassiotis, C.N., and Vlachonasios, K.E. (2025). How Biological and Environmental Factors Affect the Quality of Lavender Essential Oils. Physiologia, 5.","DOI":"10.3390\/physiologia5010011"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.jep.2012.07.016","article-title":"Antimicrobial Activity of Plants Used as Medicinals on an Indigenous Reserve in Rio Das Cobras, Paran\u00e1, Brazil","volume":"143","author":"Nocchi","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.jep.2006.12.006","article-title":"Antileishmanial and Antifungal Activity of Plants Used in Traditional Medicine in Brazil","volume":"111","author":"Braga","year":"2007","journal-title":"J. Ethnopharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1590\/S1517-83822014000300009","article-title":"Chemical Composition of Essential Oil from Ripe Fruit of Schinus terebinthifolius Raddi and Evaluation of Its Activity against Wild Strains of Hospital Origin","volume":"45","author":"Cole","year":"2014","journal-title":"Braz. J. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s00044-023-03162-3","article-title":"Enantioselectivity of Pinene against Leishmania amazonensis","volume":"33","author":"Garcia","year":"2024","journal-title":"Med. Chem. Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Monzote, L., Herrera, I., Satyal, P., and Setzer, W.N. (2019). In-Vitro Evaluation of 52 Commercially-Available Essential Oils against Leishmania amazonensis. Molecules, 24.","DOI":"10.3390\/molecules24071248"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e202401076","DOI":"10.1002\/cbdv.202401076","article-title":"Antileishmanial and Antifungal Activities of Volatile Oils from Cinnamomum cassia Bark and Schinus molle Leaves","volume":"21","author":"Fernandes","year":"2024","journal-title":"Chem. Biodivers."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"120533","DOI":"10.1016\/j.jep.2025.120533","article-title":"Effect of Schinus molle L. Essential Oil on Leishmania amazonensis: Targeting Proliferation, Mitochondrial Potential and Autophagy","volume":"354","author":"Rosa","year":"2026","journal-title":"J. Ethnopharmacol."},{"key":"ref_43","first-page":"629","article-title":"Antileishmanial Potentialities of Croton linearis Leaf Essential Oil","volume":"13","author":"EscalonaArranz","year":"2018","journal-title":"Nat. Prod. Commun."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.exppara.2013.10.007","article-title":"Essential Oil from Chenopodium ambrosioides and Main Components: Activity against Leishmania, Their Mitochondria and Other Microorganisms","volume":"136","author":"Monzote","year":"2014","journal-title":"Exp. Parasitol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Monzote, L., Scherbakov, A.M., Scull, R., Satyal, P., Cos, P., Shchekotikhin, A.E., Gille, L., and Setzer, W.N. (2020). Essential Oil from Melaleuca leucadendra: Antimicrobial, Antikinetoplastid, Antiproliferative and Cytotoxic Assessment. Molecules, 25.","DOI":"10.3390\/molecules25235514"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Monzote, L., Mach\u00edn, L., Gonz\u00e1lez, A., Scull, R., Guti\u00e9rrez, Y.I., Satyal, P., Gille, L., and Setzer, W.N. (2024). Eugenol-Rich Essential Oil from Pimenta dioica: In Vitro and in Vivo Potentialities against Leishmania amazonensis. Pharmaceuticals, 17.","DOI":"10.3390\/ph17010064"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Monzote, L., Guti\u00e9rrez, Y., Machin, L., Staniek, K., Scull, R., Satyal, P., Gille, L., and Setzer, W.N. (2020). Antileishmanial Activity and Influence on Mitochondria of the Essential Oil from Tagetes lucida Cav. and Its Main Component. Sci. Pharm., 88.","DOI":"10.3390\/scipharm88030031"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"25120","DOI":"10.1074\/jbc.M301975200","article-title":"Antimonial-Induced Increase in Intracellular Ca2+ through Non-Selective Cation Channels in the Host and the Parasite Is Responsible for Apoptosis of Intracellular Leishmania donovani Amastigotes","volume":"278","author":"Sudhandiran","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1128\/AEM.68.4.1561-1568.2002","article-title":"The Phenolic Hydroxyl Group of Carvacrol Is Essential for Action against the Food-Borne Pathogen Bacillus cereus","volume":"68","author":"Ultee","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1002\/cbdv.200900375","article-title":"Essential-Oil Composition, Antileishmanial, and Toxicity Study of Artemisia abyssinica and Satureja punctata ssp. punctata from Ethiopia","volume":"7","author":"Tariku","year":"2010","journal-title":"Chem. Biodivers."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1007\/s00436-020-06952-5","article-title":"In Vitro Antileishmanial Potentialities of Essential Oils from Citrus limon and Pistacia lentiscus Harvested in Tunisia","volume":"120","author":"Maaroufi","year":"2021","journal-title":"Parasitol. Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5761","DOI":"10.3390\/molecules19055761","article-title":"Antiparasitic Activity of Natural and Semi-Synthetic Tirucallane Triterpenoids from Schinus terebinthifolius (Anacardiaceae): Structure\/Activity Relationships","volume":"19","author":"Morais","year":"2014","journal-title":"Molecules"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Rodrigues, A.C.J., Carloto, A.C.M., Gon\u00e7alves, M.D., Concato, V.M., Detoni, M.B., DosSantos, Y.M., Cruz, E.M.S., Madureira, M.B., Nunes, A.P., and Pires, M.F.M.K. (2023). Exploring the Leishmanicidal Potential of Terpenoids: A Comprehensive Review on Mechanisms of Cell Death. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1260448"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"112981","DOI":"10.1016\/j.jep.2020.112981","article-title":"In Vitro Leishmanicidal Activity of Monoterpenes Present in Two Species of Protium (Burseraceae) on Leishmania amazonensis","volume":"259","author":"Santana","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_55","first-page":"285","article-title":"Chemical Composition and In Vitro Cytotoxic and Antileishmanial Activities of Extract and Essential Oil from Leaves of Piper cernuum","volume":"10","author":"Capello","year":"2015","journal-title":"Nat. Prod. Commun."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"13001","DOI":"10.1080\/07391102.2021.1978320","article-title":"Leishmanicidal and Cytotoxic Activity of Essential Oil from the Fruit Peel of Myrciaria floribunda (H. West Ex Willd.) O. Berg: Molecular Docking and Molecular Dynamics Simulations of Its Major Constituent onto Leishmania Enzyme Targets","volume":"40","author":"Holanda","year":"2022","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.ijpddr.2019.06.003","article-title":"Route Map for the Discovery and Pre-Clinical Development of New Drugs and Treatments for Cutaneous Leishmaniasis","volume":"11","author":"Caridha","year":"2019","journal-title":"IJP Drugs Drug Resist."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/jphp.12334","article-title":"Essential Oils and Their Constituents as Skin Penetration Enhancer for Transdermal Drug Delivery: A Review","volume":"67","author":"Herman","year":"2015","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_59","unstructured":"Adams, R.P. (2007). Identification of Essential Oil Components by Gas Chromatography\/Mass Spectrometry, Allured Publishing. [4th ed.]."},{"key":"ref_60","unstructured":"Mondello, L. (2016). FFNSC 3, Shimadzu Scientific Instruments."},{"key":"ref_61","unstructured":"National Institute of Standards and Technology (2020). NIST20 Database."},{"key":"ref_62","unstructured":"Satyal, P. (2015). Development of GC-MS Database of Essential Oil Components by the Analysis of Natural Essential Oils and Synthetic Compounds and Discovery of Biologically Active Novel Chemotypes in Essential Oils. [Ph.D. Dissertation, University of Alabama in Huntsville]."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"7087","DOI":"10.1074\/jbc.275.10.7087","article-title":"Lack of Oxidative Phosphorylation and Low Mitochondrial Membrane Potential Decrease Susceptibility to Apoptosis and Do Not Modulate the Protective Effect of Bcl-x(L) in Osteosarcoma Cells","volume":"275","author":"Dey","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/0003-2697(68)90092-4","article-title":"Estimation of Total, Protein-Bound, and Nonprotein Sulfhydryl Groups in Tissue with Ellman\u2019s Reagent","volume":"25","author":"Sedlak","year":"1968","journal-title":"Anal. Biochem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/0076-6879(90)86134-H","article-title":"[42] Determination of Aldehydic Lipid Peroxidation Products: Malonaldehyde and 4-Hydroxynonenal","volume":"186","author":"Esterbauer","year":"1990","journal-title":"Methods Enzymol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1021\/tx970180z","article-title":"Reactions of N-Methyl-2-Phenylindole with Malondialdehyde and 4- Hydroxyalkenals. Mechanistic Aspects of the Colorimetric Assay of Lipid Peroxidation","volume":"11","author":"Erdelmeier","year":"1998","journal-title":"Chem. Res. Toxicol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1055\/s-2007-979938","article-title":"Peroxidation Potential and Antioxidant Activity of Serum in Patients with Diabetes Mellitus and Myocard Infarction","volume":"27","author":"Toker","year":"1995","journal-title":"Horm. Metab. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1128\/AAC.43.5.1234","article-title":"Selective Effect of 2\u2032,6\u2032-Dihydroxy-4\u2032-Methoxychalcone Isolated from Piper aduncum on Leishmania amazonensis","volume":"43","author":"Moreira","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1128\/AAC.45.5.1349-1354.2001","article-title":"Antileishmanial Activity of an Indole Alkaloid from Peschiera australis","volume":"45","author":"Delorenzi","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/0022-1759(93)90088-O","article-title":"An Improved MTT Assay","volume":"157","author":"Sladowski","year":"1993","journal-title":"J. Immunol. 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