{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T19:55:56Z","timestamp":1781553356110,"version":"3.54.5"},"reference-count":56,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Background: Hepatic ischemia\u2013reperfusion (I\/R) injury induces systemic oxidative stress and inflammatory responses that may lead to remote lung injury. This study investigated whether taxifolin attenuates hepatic I\/R-induced lung damage and examined the involvement of the nuclear factor-\u03baB (NF-\u03baB) and high-mobility group box-1 (HMGB1) signaling axis. Methods: Twenty-eight male Wistar rats were divided into four groups (n = 7): control, taxifolin, hepatic I\/R, and taxifolin+I\/R. Serum oxidative stress markers (malondialdehyde [MDA], interleukin [IL]-6, total antioxidant\/oxidant status [TAS\/TOS]) and wet-to-dry lung weight ratio were measured. Lung tissues were evaluated histopathologically and immunohistochemically for NF-\u03baB and HMGB1 expression. Bioinformatics pathway enrichment and molecular docking analyses were also performed. Results: Hepatic I\/R significantly increased serum MDA, IL-6, and TOS levels and decreased TAS (p &lt; 0.05). Severe lung injury was observed in the hepatic I\/R group (median score: 11), whereas taxifolin pretreatment significantly reduced the injury score (median score: 5, p &lt; 0.001). NF-\u03baB and HMGB1 expression were markedly elevated following hepatic I\/R and significantly decreased with taxifolin treatment (p &lt; 0.05). A strong positive correlation was found between NF-\u03baB and HMGB1 expression (r = 0.82, p &lt; 0.001). Pathway enrichment analysis indicated involvement of Toll-like receptor (TLR)-related inflammatory signaling, and docking analysis demonstrated favorable binding of taxifolin to TLR4 and NF-\u03baB p65. Conclusion: Taxifolin attenuated hepatic I\/R-induced lung injury by reducing oxidative stress and suppressing HMGB1\u2013TLR4\u2013NF-\u03baB-mediated inflammatory signaling.<\/jats:p>","DOI":"10.3390\/molecules31071134","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T09:57:24Z","timestamp":1774864644000},"page":"1134","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Taxifolin Attenuates Remote Lung Injury Induced by Hepatic Ischemia\u2013Reperfusion in Rats"],"prefix":"10.3390","volume":"31","author":[{"given":"Serkan","family":"Erbatur","sequence":"first","affiliation":[{"name":"Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Dicle University, 21280 Diyarbak\u0131r, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meral Erdal","family":"Erbatur","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Reanimation, Faculty of Medicine, Dicle University, 21280 Diyarbak\u0131r, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u0131rat","family":"\u015eahin","sequence":"additional","affiliation":[{"name":"Division of Infertility, Turan \u00c7etin In-Vitro Fertilization Center, \u00c7ukurova, 01360 Adana, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcseyin","family":"Bilge","sequence":"additional","affiliation":[{"name":"Division of General Surgery, Gazi Ya\u015fargil Training and Research Hospital, 21010 Diyarbak\u0131r, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0547-4139","authenticated-orcid":false,"given":"Aysun","family":"Ekinci","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Dicle University, 21280 Diyarbakir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/S0002-9610(00)00573-0","article-title":"Hepatic ischemia-reperfusion injury","volume":"181","author":"Habib","year":"2001","journal-title":"Am. 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