{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T18:48:53Z","timestamp":1788374933964,"version":"build-2803163510"},"reference-count":55,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education and Research, Romania, UEFISCDI Agency","award":["PN-IV-P7-7_1-PED-2024-2326\/08.01.2025"],"award-info":[{"award-number":["PN-IV-P7-7_1-PED-2024-2326\/08.01.2025"]}]},{"name":"Ministry of Education and Research, Romania, UEFISCDI Agency","award":["PN-IV-P6-6.1-CoEx-2024-0196\/09.02.2026"],"award-info":[{"award-number":["PN-IV-P6-6.1-CoEx-2024-0196\/09.02.2026"]}]},{"DOI":"10.13039\/501100006476","name":"Romanian Academy","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006476","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/0561n6946","id-type":"ROR","asserted-by":"publisher"}]},{"name":"EU\u2019s NextGenerationEU instrument through the National Recovery and Resilience Plan of Romania (PNRR)\u2014Pillar III-C9-I8","award":["760086\/23.05.2023"],"award-info":[{"award-number":["760086\/23.05.2023"]}]},{"name":"EU\u2019s NextGenerationEU instrument through the National Recovery and Resilience Plan of Romania (PNRR)\u2014Pillar III-C9-I8","award":["CF 43\/14.11.2023"],"award-info":[{"award-number":["CF 43\/14.11.2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In this study, we investigated the chemical composition, antimicrobial and antibiofilm activities, and cytotoxicity of Thuja occidentalis L. (thuja) and Myrtus communis L. (myrtle) essential oils (EOs) to evaluate their potential as natural alternatives for topical applications. Thuja EOs were extracted from fresh and dried leaves and cones, while myrtle EO was extracted from fresh leaves. GC-MS analysis revealed that myrtle EO was rich in oxygenated monoterpenes (58.2%), predominantly eucalyptol (41.86%) and \u03b1-pinene (20.98%). In contrast, thuja EOs were dominated by monoterpene hydrocarbons (55\u201382%), with \u03b1-pinene as the major component (29\u201366%), and their composition varied markedly with plant organ and moisture state. Myrtle EO displayed the most potent and broad-spectrum antimicrobial activity, with MIC values as low as 3.096 \u00b5L\/mL against Staphylococcus aureus and Escherichia coli, and effectively inhibited microbial adherence. Thuja EOs showed selective activity, particularly against Gram-positive bacteria and Candida parapsilosis, with EOs extracted from fresh leaves and cones exhibiting the lowest MICs (1.95\u20137.8 \u00b5L\/mL). All EOs did not show cytotoxicity to human keratinocytes at concentrations \u2264125 \u00b5g\/mL and, when diluted to 0.05%, indicated excellent skin compatibility in human patch tests. This study suggests that myrtle and thuja EOs, particularly myrtle, are promising and safe natural antimicrobial agents for dermatological and cosmetic formulations.<\/jats:p>","DOI":"10.3390\/molecules31071225","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T14:00:31Z","timestamp":1775570431000},"page":"1225","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Chemical Characterization, Evaluation of Antimicrobial Potential, and Cytotoxic Activity of Thuja occidentalis L. and Myrtus communis L. Essential Oils for Topical Applications"],"prefix":"10.3390","volume":"31","author":[{"given":"Elena","family":"D\u0103nil\u0103","sequence":"first","affiliation":[{"name":"INCDTP\u2014Leather and Footwear Research Institute, Collagen Department, 031215 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7342-5398","authenticated-orcid":false,"given":"Ioana Cristina","family":"Marinas","sequence":"additional","affiliation":[{"name":"The Research Institute of the University of Bucharest (ICUB), 050107 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4196-8160","authenticated-orcid":false,"given":"Madalina Diana","family":"Gaboreanu","sequence":"additional","affiliation":[{"name":"The Research Institute of the University of Bucharest (ICUB), 050107 Bucharest, Romania"},{"name":"Botany and Microbiology Department, Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, 050095 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2254-0799","authenticated-orcid":false,"given":"Vlad Andrei","family":"Neac\u0219u","sequence":"additional","affiliation":[{"name":"Faculty of Chemical Engineering and Biotechnology, National University of Science and Technology Politehnica Bucharest, 1\u20137 Gh. Polizu Street, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Titorencu","sequence":"additional","affiliation":[{"name":"Institute of Cellular Biology and Pathology \u201cNicolae Simionescu\u201d, 8 B. P. Hasdeu Street, District 5, 050568 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minodora Maria","family":"Marin","sequence":"additional","affiliation":[{"name":"Advanced Polymer Materials Group, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania"},{"name":"Research Institute for Advanced Materials, Products, and Processes (CAMPUS), National University of Science and Technology Politehnica Bucharest, 060042 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3544-9214","authenticated-orcid":false,"given":"Durmu\u015f Alpaslan","family":"Kaya","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Hatay Mustafa Kemal University, 31060 Antakya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0467-1034","authenticated-orcid":false,"given":"Nafiz","family":"\u00c7elikta\u015f","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Hatay Mustafa Kemal University, 31060 Antakya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9960-2244","authenticated-orcid":false,"given":"M\u0103d\u0103lina","family":"Albu Kaya","sequence":"additional","affiliation":[{"name":"INCDTP\u2014Leather and Footwear Research Institute, Collagen Department, 031215 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raluca","family":"\u021au\u021buianu","sequence":"additional","affiliation":[{"name":"Institute of Cellular Biology and Pathology \u201cNicolae Simionescu\u201d, 8 B. 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