{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:57:34Z","timestamp":1785427054351,"version":"3.56.0"},"reference-count":90,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Polish Ministry of Science and Higher Education re-search project within the University of Rzesz\u00f3w","award":["PB\/KCHTZ\/2026"],"award-info":[{"award-number":["PB\/KCHTZ\/2026"]}]},{"award":["PB\/KCHTZ\/2026"],"award-info":[{"award-number":["PB\/KCHTZ\/2026"]}],"id":[{"id":"https:\/\/ror.org\/03pfsnq21","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Centre of Science","award":["2025\/09\/X\/NZ9\/01029"],"award-info":[{"award-number":["2025\/09\/X\/NZ9\/01029"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Gemmotherapy is a branch of phytotherapy based on the use of extracts derived from plant meristematic tissues, including buds, young shoots, and sprouts. Due to their embryonic nature and high metabolic activity, these tissues constitute a concentrated source of bioactive compounds such as polyphenols, phytohormones, amino acids, vitamins, and enzymes. The unique phytochemical profile of bud extracts, together with synergistic interactions within the phytocomplex, contributes to their enhanced biological activity compared to mature plant materials. This review provides a comprehensive overview of the current state of knowledge on the chemical composition, extraction technologies, and biological properties of gemmotherapeutic preparations. Particular attention is given to both traditional and modern extraction methods, including glycerin maceration and pulsed ultrasound-assisted extraction, as well as factors affecting the quality and variability of the obtained extracts. Available evidence indicates that gemmotherapeutic preparations exhibit a broad spectrum of biological activities, including antioxidant, anti-inflammatory, immunomodulatory, antimicrobial, and anticancer effects. These properties suggest their potential application as supportive agents in the management of chronic inflammatory diseases, metabolic disorders, and infections, as well as in functional foods and natural cosmetics. However, the lack of standardized production protocols, variability of raw materials, and limited clinical evidence remain significant challenges. Further research focusing on advanced analytical techniques, metabolomic profiling, and clinical validation is essential for the integration of gemmotherapy into evidence-based medicine.<\/jats:p>","DOI":"10.3390\/molecules31101559","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T19:35:43Z","timestamp":1778528143000},"page":"1559","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bioactive Compounds and Therapeutic Potential of Plant Buds: Current Evidence and Future Perspectives of Gemmotherapy"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4010-4352","authenticated-orcid":false,"given":"Monika","family":"Tomczyk","sequence":"first","affiliation":[{"name":"Department of Chemistry and Food Toxicology, Faculty of Technology and Life Sciences, University of Rzesz\u00f3w, \u0106wikli\u0144skiej 1a, 35-601 Rzeszow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1601-6296","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"D\u017cugan","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Food Toxicology, Faculty of Technology and Life Sciences, University of Rzesz\u00f3w, \u0106wikli\u0144skiej 1a, 35-601 Rzeszow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Charpentier, T., Boisard, S., Le Ray, A.-M., Br\u00e9ard, D., Chabrier, A., Esselin, H., Guilet, D., Ripoll, C., and Richomme, P. (2022). A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS2 Method\u2014Application to Alnus glutinosa, Ribes nigrum, Rosa canina, Rosmarinus officinalis and Tilia tomentosa. Plants, 11.","DOI":"10.3390\/plants11020144"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Rao, A.V., and Rao, L.G. (2015). Bud Extracts as New Phytochemical Source for Herbal Preparations\u2014Quality Control and Standardization by Analytical Fingerprint. Phytochemicals\u2014Isolation, Characterisation and Role in Human Health, IntechOpen.","DOI":"10.5772\/58648"},{"key":"ref_3","first-page":"24","article-title":"Buds, leaves, and seeds of blackcurrant\u2014Source of bioactive substances with pro-health properties","volume":"25","year":"2018","journal-title":"\u017bYWNO\u015a\u0106 Nauka Technol. Jako\u015b\u0107"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.1016\/j.phytochem.2007.06.028","article-title":"Phytochemistry and pharmacognosy","volume":"68","author":"Phillipson","year":"2007","journal-title":"Phytochemistry"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Di Vito, M., Gentile, M., Mattarelli, P., Barbanti, L., Micheli, L., Mazzuca, C., Garzoli, S., Titubante, M., Vitali, A., and Cacaci, M. (2020). Phytocomplex Influences Antimicrobial and Health Properties of Concentrated Glycerine Macerates. Antibiotics, 9.","DOI":"10.3390\/antibiotics9120858"},{"key":"ref_6","unstructured":"Nicoletti, M., and Piter\u00e0 di Clima, F. (2020). Gemmotherapy, and the Scientific Foundations of a Modern Meristemotherapy, Cambridge Scholars Publishing."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Raiciu, A.-D. (2019). Gemmotherapy\u2014Modern Medicine. Proceedings, 29.","DOI":"10.3390\/proceedings20190290117"},{"key":"ref_8","unstructured":"Andrianne, F. (2012). Treatise on Gemmotherapy: The Therapeutic Use of Buds, Editions Amyris."},{"key":"ref_9","first-page":"21","article-title":"The Research in Meristemotherapy\u2014Past and Perspectives","volume":"19","author":"Ardelean","year":"2016","journal-title":"J. Med. Arad."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Wojdy\u0142o, A., Nowicka, P., Grimalt, M., Legua, P., Almansa, M.S., Amor\u00f3s, A., Carbonell-Barrachina, \u00c1.A., and Hern\u00e1ndez, F. (2019). Polyphenol Compounds and Biological Activity of Caper (Capparis spinosa L.) Flowers Buds. Plants, 8.","DOI":"10.3390\/plants8120539"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Donno, D., Mellano, M.G., Cerutti, A.K., and Beccaro, G.L. (2016). Biomolecules and Natural Medicine Preparations: Analysis of New Sources of Bioactive Compounds from Ribes and Rubus spp. Buds. Pharmaceuticals, 9.","DOI":"10.3390\/ph9010007"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1007\/s13197-015-2115-6","article-title":"Phytochemical fingerprint and chemometrics for natural food preparation pattern recognition: An innovative technique in food supplement quality control","volume":"53","author":"Donno","year":"2015","journal-title":"J. Food Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jpba.2015.06.004","article-title":"Phenolic composition of \u201cbud extracts\u201d of Ribes nigrum L., Rosa canina L. and Tilia tomentosa M","volume":"115","author":"Ieri","year":"2015","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_14","first-page":"85","article-title":"Hystopathological and clinical investigations of five gemmo-derivatives from plants and their biotherapeutical properties","volume":"4","author":"Raiciu","year":"2016","journal-title":"Acad. Res. J. Agric. Sci. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.foodchem.2014.03.056","article-title":"Flavonol glycosides and other phenolic compounds in buds and leaves of different varieties of black currant (Ribes nigrum L.) and changes during growing season","volume":"160","author":"Liu","year":"2014","journal-title":"Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Turrini, F., Donno, D., Beccaro, G.L., Pittaluga, A., Grilli, M., Zunin, P., and Boggia, R. (2020). Bud-Derivatives, a Novel Source of Polyphenols and How Different Extraction Processes Affect Their Composition. Foods, 9.","DOI":"10.3390\/foods9101343"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"El-Saadony, M.T., Saad, A.M., Mohammed, D.M., Alkafaas, S.S., Abd El-Mageed, T.A., Fahmy, M.A., Ezzat Ahmed, A., Algopishi, U.B., Abu-Elsaoud, A.M., and Mosa, W.F.A. (2025). Plant bioactive compounds: Extraction, biological activities, immunological, nutritional aspects, food application, and human health benefits-A comprehensive review. Front. Nutr., 12.","DOI":"10.3389\/fnut.2025.1659743"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Negreanu-Pirjol, B.-S., Negreanu-Pirjol, T., Busuricu, F., Jurja, S., Craciunescu, O., Oprea, O., Motelica, L., Oprita, E.I., and Roncea, F.N. (2024). The Role of Antioxidant Plant Extracts\u2019 Composition and Encapsulation in Dietary Supplements and Gemmo-Derivatives, as Safe Adjuvants in Metabolic and Age-Related Conditions: A Review. Pharmaceuticals, 17.","DOI":"10.3390\/ph17121738"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"574","DOI":"10.3390\/nutraceuticals3040041","article-title":"Influence of Maceration Solvent on Chemical Composition of Gemmotherapy Macerates\u2014A Case Study on Olea europaea Young Shoots","volume":"3","author":"Esselin","year":"2023","journal-title":"Nutraceuticals"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"H\u00e9jja, M., Mihok, E., Alaya, A., Jolji, M., Gy\u00f6rgy, \u00c9., Meszaros, N., Turcus, V., Ol\u00e1h, N.K., and M\u00e1th\u00e9, E. (2024). Specific Antimicrobial Activities Revealed by Comparative Evaluation of Selected Gemmotherapy Extracts. Antibiotics, 13.","DOI":"10.3390\/antibiotics13020181"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"H\u00e9jja, M., Gy\u00f6rgy, \u00c9., L\u00f3ga, F.\u00c1., Nagy, R., Pacza, T., Sipos, P., Tank\u00f3, G., Laslo, \u00c9., M\u00e9sz\u00e1ros, N., and Turcu\u0219, V. (2025). Gemmotherapy Extracts Like the Dog Rose, Lingonberry, Sea Buckthorn, Blackthorn, Common Grape, Hawthorn, Raspberry and Boxwood Feature Variable Yet Excelling Antimicrobial Effects. Antibiotics, 14.","DOI":"10.3390\/antibiotics14101052"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Stanciu, G., Aonofriesei, F., Lupsor, S., Oancea, E., and Mititelu, M. (2023). Chemical Composition, Antioxidant Activity, and Antibacterial Activity of Black Poplar Buds\u2019 Hydroalcoholic Macerates from Dobrogea Area. Molecules, 28.","DOI":"10.3390\/molecules28134920"},{"key":"ref_23","first-page":"163","article-title":"Tilia tomentosa foliar bud extract: Phytochemical analysis and dermatological testing","volume":"19","author":"Peev","year":"2009","journal-title":"Stud. Univ. Vasile Goldi\u015f Ser. \u015etiin\u0163ele Vie\u0163ii"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Krauze-Baranowska, M., G\u0142\u00f3d, D., Kula, M., Majdan, M., Ha\u0142asa, R., Matkowski, A., Koz\u0142owska, W., and Kawiak, A. (2014). Chemical composition and biological activity of Rubus idaeus shoots\u2014A traditional herbal remedy of Eastern Europe. BMC Complement. Altern. Med., 14.","DOI":"10.1186\/1472-6882-14-480"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2863","DOI":"10.1002\/jsfa.6627","article-title":"Castanea spp. buds as a phytochemical source for herbal preparations: Botanical fingerprint for nutraceutical identification and functional food standardization","volume":"94","author":"Donno","year":"2014","journal-title":"J. Sci. Food Agric."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"19","DOI":"10.26353\/j.itahort\/2020.1.1929","article-title":"Phytochemicals from Castanea spp. buds and green extraction technologies: The Finnover project","volume":"27","author":"Donno","year":"2020","journal-title":"Italus Hortus"},{"key":"ref_27","first-page":"735","article-title":"Antimicrobial activity of Ribes nigrum, Rosmarinus officinalis, Betula pubescens, Salix alba, Vaccinium myrtillus gemoderivatives","volume":"58","author":"Raiciu","year":"2010","journal-title":"Farmacia"},{"key":"ref_28","first-page":"2519","article-title":"Phytohormones in Sweet Cherry Buds During Winter Rest and Bud Development","volume":"42","author":"Chmielewski","year":"2022","journal-title":"J. Plant Growth Regul."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.37358\/RC.17.7.5731","article-title":"Ficus carica SSP Dottato Buds by Intercropping Different Species: Metabolites, Antioxidant Activity and Endogenous Plant Hormones (IAA, ABA)","volume":"68","author":"Toma","year":"2017","journal-title":"Rev. Chim."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"930","DOI":"10.3906\/tar-1407-164","article-title":"Study on the expression of dehydrin genes and activities of antioxidative enzymes in floral buds of two sand pear (Pyrus pyrifolia Nakai) cultivars requiring different chilling hours for bud break","volume":"39","author":"Hussain","year":"2015","journal-title":"Turk. J. Agric. For."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Tan, G.-F., Zhao, Q., Wang, F., Li, S.-Y., Liu, Z.-Y., Zhang, X.-Q., Zhong, X.-L., Zhu, S.-H., Lei, X.-J., and Han, Z.-M. (2025). Mechanistic insights into nutrient profiles, cellulose, and hemicellulose dynamics in red and green Toona sinensis buds during cold storage. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1518924"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"T\u00e9gl\u00e1s, T., Mihok, E., Czi\u00e1ky, Z., Ol\u00e1h, N.-K., Nyakas, C., and M\u00e1th\u00e9, E. (2023). The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-\u03b1 Level: Pilot Study. Molecules, 28.","DOI":"10.3390\/molecules28083571"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.3109\/13880209.2013.786101","article-title":"Botanicals in Ribes nigrum bud-preparations: An analytical fingerprinting to evaluate the bioactive contribution to total phytocomplex","volume":"51","author":"Donno","year":"2013","journal-title":"Pharm. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Theodosis-Nobelos, P., and Rekka, E.A. (2024). The Antioxidant Potential of Vitamins and Their Implication in Metabolic Abnormalities. Nutrients, 16.","DOI":"10.3390\/nu16162740"},{"key":"ref_35","unstructured":"Rabeea, H., Fadhil, F., and Fayyad, H. (2025, January 26). Broad-Spectrum Antibacterial Activity of the Buds Rosa Damascena Bud Extracts Against Pathogens Isolated Clinically. Proceedings of the 13th International Conference on Appliedon Applied Innovations in IT (ICAIIT), Koethen, Germany."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.jfoodeng.2013.01.014","article-title":"Techniques for extraction of bioactive compounds from plant materials: A review","volume":"117","author":"Azmir","year":"2013","journal-title":"J. Food Eng."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"8615","DOI":"10.3390\/ijms13078615","article-title":"Green Extraction of Natural Products: Concept and Principles","volume":"13","author":"Chemat","year":"2012","journal-title":"Int. J. Mol. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.aca.2012.12.019","article-title":"Natural deep eutectic solvents as new potential media for green technology","volume":"766","author":"Dai","year":"2013","journal-title":"Anal. Chim. Acta"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Turrini, F., Donno, D., Beccaro, G.L., Zunin, P., Pittaluga, A., and Boggia, R. (2019). Pulsed Ultrasound-Assisted Extraction as an Alternative Method to Conventional Maceration for the Extraction of the Polyphenolic Fraction of Ribes nigrum Buds: A New Category of Food Supplements Proposed by The FINNOVER Project. Foods, 8.","DOI":"10.3390\/foods8100466"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.foodres.2018.12.018","article-title":"An innovative green extraction and re-use strategy to valorize food supplement by-products: Castanea sativa bud preparations as case study","volume":"115","author":"Turrini","year":"2019","journal-title":"Food Res. Int."},{"key":"ref_41","first-page":"109","article-title":"Gemmotherapy extracts with quercetin and its derivatives","volume":"33","author":"Olah","year":"2023","journal-title":"Stud. Univ. Vasile Goldi\u015f Ser. \u015etiin\u0163ele Vie\u0163ii"},{"key":"ref_42","first-page":"72","article-title":"The effectiveness of gemmotherapy in current medical practice","volume":"18","year":"2015","journal-title":"Arad. Med. J."},{"key":"ref_43","first-page":"e02509","article-title":"Conventional and modern techniques for bioactive compounds recovery from plants: Review","volume":"27","author":"Mungwari","year":"2025","journal-title":"Sci. Afr."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1016\/j.foodchem.2007.03.005","article-title":"Optimisation of Extraction of Phenolics and Antioxidants from Black Currant Leaves and Buds and of Stability during Storage","volume":"105","author":"Tabart","year":"2007","journal-title":"Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Seyyedi-Mansour, S., Donn, P., Carpena, M., Chamorro, F., Barciela, P., Perez-Vazquez, A., Jorge, A.O.S., and Prieto, M.A. (2024). Utilization of Ultrasonic-Assisted Extraction for Bioactive Compounds from Floral Sources. Biol. Life Sci. Forum, 40.","DOI":"10.3390\/blsf2024040015"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.tifs.2012.03.003","article-title":"Recovery of high added-value components from food wastes: Conventional, emerging technologies and commercialized applications","volume":"26","author":"Galanakis","year":"2012","journal-title":"Trends Food Sci. Technol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1080\/14620316.2002.11511528","article-title":"Variation during dormancy and the effect of freezing and postharvest incubation on the chemical composition of blackcurrant buds (Ribes nigrum L.)","volume":"77","author":"Garland","year":"2002","journal-title":"J. Hortic. Sci. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"10501","DOI":"10.1021\/jf303398z","article-title":"An Optimized Method for Analysis of Phenolic Compounds in Buds, Leaves, and Fruits of Black Currant (Ribes nigrum L.)","volume":"60","author":"Vagiri","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.phymed.2008.12.018","article-title":"Synergy research: Approaching a new generation of phytopharmaceuticals","volume":"16","author":"Wagner","year":"2009","journal-title":"Phytomedicine"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"137","DOI":"10.5114\/bta.2024.139753","article-title":"Phytochemical composition, antioxidant, antibacterial, and enzyme inhibitory activities of organic extracts from flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry","volume":"105","author":"Thanh","year":"2024","journal-title":"BioTechnologia"},{"key":"ref_51","first-page":"117","article-title":"Antioxidant Capacity of Blackcurrant (Ribes nigrum L.) Leaves and Buds","volume":"14","author":"Kis","year":"2021","journal-title":"Acta Univ. Sapientiae Aliment."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"682","DOI":"10.3390\/antiox4040682","article-title":"Olive Tree (Olea europeae L.) Leaves: Importance and Advances in the Analysis of Phenolic Compounds","volume":"4","author":"Abaza","year":"2015","journal-title":"Antioxidants"},{"key":"ref_53","first-page":"155","article-title":"Pomegranate Leaves, Buds, and Flowers: Phytochemical, Antioxidant, and Comparative Solvent Analyzes","volume":"13","author":"Tekin","year":"2025","journal-title":"Foods Raw Mater."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1093\/ajcn\/79.5.727","article-title":"Polyphenols: Food sources and bioavailability","volume":"79","author":"Manach","year":"2004","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"7313","DOI":"10.3390\/molecules15107313","article-title":"Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties","volume":"15","author":"Dai","year":"2010","journal-title":"Molecules"},{"key":"ref_56","first-page":"45","article-title":"Plant extracts from meristematic tissues (foliar buds and shoots): Antioxidant and therapeutic action","volume":"20","author":"Militaru","year":"2010","journal-title":"Stud. Univ. Vasile Goldi\u015f Ser. \u015etiin\u0163ele Vie\u0163ii"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Albano, G.D., Gagliardo, R.P., Montalbano, A.M., and Profita, M. (2022). Overview of the Mechanisms of Oxidative Stress: Impact in Inflammation of the Airway Diseases. Antioxidants, 11.","DOI":"10.3390\/antiox11112237"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Muscolo, A., Mariateresa, O., Giulio, T., and Mariateresa, R. (2024). Oxidative Stress: The Role of Antioxidant Phytochemicals in the Prevention and Treatment of Diseases. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25063264"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Kalogerakou, T., and Antoniadou, M. (2024). The Role of Dietary Antioxidants, Food Supplements and Functional Foods for Energy Enhancement in Healthcare Professionals. Antioxidants, 13.","DOI":"10.3390\/antiox13121508"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Prata, C., Angeloni, C., and Maraldi, T. (2024). Strategies to Counteract Oxidative Stress and Inflammation in Chronic-Degenerative Diseases 2.0. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25095026"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4290","DOI":"10.1021\/jf0502698","article-title":"Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements","volume":"53","author":"Prior","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"G\u0103l\u0103\u0163anu, M.L., Pan\u0163uroiu, M., Cima, L.M., Neculai, A.M., P\u0103nu\u015f, E., Bleotu, C., Enescu, C.M., Mircioiu, I., Gavriloaia, R.M., and Auric\u0103, S.N. (2025). Polyphenolic Composition, Antioxidant Activity, and Cytotoxic Effect of Male Floral Buds from Three Populus Species Growing in the South of Romania. Molecules, 30.","DOI":"10.3390\/molecules30040913"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1007\/s00210-002-0553-y","article-title":"Effects of prodelphinidins isolated from Ribes nigrum on chondrocyte metabolism and COX activity","volume":"365","author":"Garbacki","year":"2002","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"key":"ref_64","first-page":"12","article-title":"Phytotherapy in the treatment of infectious diseases","volume":"2","author":"Ohanme","year":"2024","journal-title":"Glob. J. Clin. Med. Med. Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"28045","DOI":"10.1074\/jbc.M109.035329","article-title":"LANCL2 Is Necessary for Abscisic Acid Binding and Signaling in Human Granulocytes and in Rat Insulinoma Cells","volume":"284","author":"Sturla","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"4783","DOI":"10.1096\/fj.15-277731","article-title":"Microgram Amounts of Abscisic Acid in Fruit Extracts Improve Glucose Tolerance and Reduce Insulinemia in Rats and in Humans","volume":"29","author":"Magnone","year":"2015","journal-title":"FASEB J."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.jep.2015.06.016","article-title":"Bud extracts from Tilia tomentosa Moench inhibit hippocampal neuronal firing through GABAA and benzodiazepine receptors activation","volume":"172","author":"Allio","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.neuropharm.2011.08.040","article-title":"The GABA system in anxiety and depression and its therapeutic potential","volume":"62","year":"2012","journal-title":"Neuropharmacology"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.3390\/ph6121451","article-title":"Effect of Essential Oils on Pathogenic Bacteria","volume":"6","author":"Nazzaro","year":"2013","journal-title":"Pharmaceuticals"},{"key":"ref_70","first-page":"74","article-title":"The Effect of Gemmoderivatives on Probiotic and Pathogenic Microorganisms","volume":"52","author":"Dinu","year":"2019","journal-title":"Anim. Sci. Biotechnol."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Oki\u0144czyc, P., Widelski, J., Nowak, K., Radwan, S., W\u0142odarczyk, M., Ku\u015b, P.M., Susniak, K., and Korona-G\u0142owniak, I. (2024). Phytochemical Profiles and Antimicrobial Activity of Selected Populus spp. Bud Extracts. Molecules, 29.","DOI":"10.3390\/molecules29020437"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.ijfoodmicro.2004.03.022","article-title":"Essential oils: Their antibacterial properties and potential applications in foods\u2014A review","volume":"94","author":"Burt","year":"2004","journal-title":"Int. J. Food Microbiol."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Isidorov, V., Szoka, \u0141., and Nazaruk, J. (2018). Cytotoxicity of white birch bud extracts: Perspectives for therapy of tumours. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0201949"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.jff.2012.01.009","article-title":"Radical scavenging activity of black currant (Ribes nigrum L.) extract and its inhibitory effect on gastric cancer cell proliferation via induction of apoptosis","volume":"4","author":"Jia","year":"2012","journal-title":"J. Funct. Foods"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Chaudhry, G.-S., Md Akim, A., Sung, Y.Y., and Sifzizul, T.M.T. (2022). Cancer and apoptosis: The apoptotic activity of plant and marine natural products and their potential as targeted cancer therapeutics. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.842376"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"100396","DOI":"10.1016\/j.phyplu.2022.100396","article-title":"Anticancer properties and mechanisms of botanical derivatives","volume":"3","author":"Banerjee","year":"2023","journal-title":"Phytomed. Plus"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1039\/D4RA05089D","article-title":"Incredible use of plant-derived bioactives as anticancer agents","volume":"15","author":"Kangra","year":"2025","journal-title":"RSC Adv."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Ekor, M. (2014). The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Front. Pharmacol., 4.","DOI":"10.3389\/fphar.2013.00177"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"3240","DOI":"10.1038\/s41598-021-82207-2","article-title":"Assessment of cytotoxicity and antioxidant properties of berry leaves as by-products with potential application in cosmetic and pharmaceutical products","volume":"11","author":"Ziemlewska","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_80","first-page":"6","article-title":"The pharmacovigilance of complementary medicines: Unpacking the complexities","volume":"92","author":"Beer","year":"2025","journal-title":"S. Afr. Pharm. J."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1111\/bcp.13496","article-title":"Nutraceuticals: Opening the debate for a regulatory framework","volume":"84","author":"Santini","year":"2018","journal-title":"Br. J. Clin. Pharmacol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"2986","DOI":"10.1016\/j.phytochem.2007.09.014","article-title":"Nutraceuticals: Facts and fiction","volume":"68","year":"2007","journal-title":"Phytochemistry"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1038\/nrm.2016.25","article-title":"Metabolomics: Beyond biomarkers and towards mechanisms","volume":"17","author":"Johnson","year":"2016","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1152\/physrev.00035.2018","article-title":"Metabolomics for Investigating Physiological and Pathophysiological Processes","volume":"99","author":"Wishart","year":"2019","journal-title":"Physiol. Rev."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Turrini, F., Donno, D., Grasso, F., Mustorgi, E., Beccaro, G.L., Guido, M., Fior, T., Grilli, M., Pittaluga, A., and Boggia, R. (2023). EEM Fluorescence Spectroscopy Coupled with HPLC-DAD Analysis for the Characterization of Bud Derivative Dietary Supplements: A Preliminary Introduction to GEMMAPP, the Free Data-Repository from the FINNOVER Project. Appl. Sci., 13.","DOI":"10.3390\/app13158679"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Michalak, M. (2023). Plant Extracts as Skin Care and Therapeutic Agents. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242015444"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"G\u01celb\u01ceu, C.-\u015e., Irimie, M., Neculau, A.E., Dima, L., Poga\u010dnik da Silva, L., V\u00e2rciu, M., and Badea, M. (2024). The Potential of Plant Extracts Used in Cosmetic Product Applications\u2014Antioxidants Delivery and Mechanism of Actions. Antioxidants, 13.","DOI":"10.3390\/antiox13111425"},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Ziemlewska, A., Nizio\u0142-\u0141ukaszewska, Z., Zag\u00f3rska-Dziok, M., Bujak, T., W\u00f3jciak, M., and Sowa, I. (2022). Evaluation of Cosmetic and Dermatological Properties of Kombucha-Fermented Berry Leaf Extracts Considered to Be By-Products. Molecules, 27.","DOI":"10.3390\/molecules27072345"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Salazar, J., Ortega, \u00c1., P\u00e9rez, J.L., Garrido, B., Santeliz, R., Galb\u00e1n, N., D\u00edaz, M.P., Cano, R., Cano, G., and Contreras-Velasquez, J.C. (2025). Role of Polyphenols in Dermatological Diseases: Exploring Pharmacotherapeutic Mechanisms and Clinical Implications. Pharmaceuticals, 18.","DOI":"10.3390\/ph18020247"},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Domaga\u0142a, B., Orli\u0144ska, J., Duda, M., Setkowicz-Janeczko, Z., Starzyk, M., Piasna-S\u0142upecka, E., Drozdowska, M., and Godos, E. (2025). Natural Phytotherapeutics in Dermatology and Cosmetology: Bioactive Potential of Grape Pomace on Human Skin Fibroblasts. 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