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We previously demonstrated that kujiol A exhibited multiple biological activities, including the inhibition of Ca2+ signal transduction. In the present study, we found that kujiol A prevented the transcription of interferon-\u03b3 (IFN-\u03b3), interleukin (IL)-2, IL-4, and Fas ligand in T-box transcription factor Eomesodermin (Eomes)-transfected murine T cell lymphoma BW5147 cells stimulated with phorbol ester and ionomycin (a calcium ionophore). In the murine cytotoxic T cell line CTLL-2, kujiol A reduced ionomycin-induced increases in IFN-\u03b3 mRNA expression. Luciferase reporter assays revealed that kujiol A inhibited the transcriptional activities of nuclear factor of activated T cells (NFAT) and, to a lesser extent, nuclear factor \u03baB in human embryonic kidney 293T cells. Kujiol A did not affect NFATc2 (also known as NFAT1) protein expression. However, a chromatin immunoprecipitation assay showed that kujiol A prevented the NFATc2 protein from interacting with the IFN-\u03b3 and IL-2 promoters in Eomes-transfected BW5147 cells. Collectively, these results demonstrate that kujiol A is a potent immunosuppressant that inhibits T cell cytokine expression by targeting the NFAT pathway.<\/jats:p>","DOI":"10.3390\/molecules31101613","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T13:10:42Z","timestamp":1778505042000},"page":"1613","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Kujiol A Inhibits Interferon-\u03b3 and Interleukin-2 Expression and the NFATc2 Interaction with Their Promoters in T Cells"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6197-2329","authenticated-orcid":false,"given":"Tanpitcha","family":"Yodweerapong","sequence":"first","affiliation":[{"name":"Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikako","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaka","family":"Nakao","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9840-6708","authenticated-orcid":false,"given":"Sakihito","family":"Kitajima","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9312-1298","authenticated-orcid":false,"given":"Tomoo","family":"Shiba","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken-ichi","family":"Kimura","sequence":"additional","affiliation":[{"name":"The United Graduate School of Agricultural Sciences, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6000-4500","authenticated-orcid":false,"given":"Takao","family":"Kataoka","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan"},{"name":"Center for Social and Biomedical Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Castro, F., Cardoso, A.P., Gon\u00e7alves, R.M., Serre, K., and Oliveira, M.J. 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