{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T05:09:16Z","timestamp":1779253756245,"version":"3.51.4"},"reference-count":18,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study comparatively evaluated how solvent polarity affects the chemical antioxidant capacity, preliminary hyaluronidase inhibition, and supportive chemical features of Buddleja officinalis Maxim. Six extracts prepared with petroleum ether, ethyl acetate, n-butanol, water, 60% ethanol, and 95% ethanol were assessed using DPPH\u00b7, ABTS+\u00b7, ferric reducing power, and hyaluronidase inhibition assays, together with total flavonoid and linarin determinations. The 60% ethanol extract showed the strongest overall radical-scavenging activity, reaching 95% DPPH\u00b7 scavenging and 95% ABTS+\u00b7 scavenging at 2 mg\/mL, whereas the 95% ethanol extract showed the highest ferric reducing power under the tested conditions. Total flavonoid and linarin contents were highest in the ethanol-rich fractions, especially the 95% ethanol and 60% ethanol extracts. In the hyaluronidase assay, both the petroleum ether and 60% ethanol extracts showed relatively strong inhibition at 2.5 mg\/mL, with inhibition rates of 74% and 68%, respectively, suggesting that different chemical classes may contribute to this endpoint. Supportive 1H NMR and UPLC\u2013QTOF\u2013MS data indicated clear polarity-dependent compositional differences; the 60% ethanol extract was enriched in phenylethanoid glycosides and flavonoid glycosides, whereas the petroleum ether extract showed predominantly lipophilic features. Overall, medium-polarity phenolic-rich fractions were more closely associated with chemical antioxidant capacity, while hyaluronidase inhibition may involve contributions from both non-polar and medium-polarity constituents. The present extract-level comparison provides a useful basis for fraction selection, extract standardization, and follow-up activity-guided studies.<\/jats:p>","DOI":"10.3390\/molecules31101706","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T10:25:16Z","timestamp":1779099916000},"page":"1706","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Solvent Polarity Shapes Antioxidant Capacity, Preliminary Hyaluronidase Inhibition, and Chemical Profile of Buddleja officinalis Extracts"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7621-293X","authenticated-orcid":false,"given":"Gang","family":"Tian","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan 467000, China"},{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Pingdingshan 467000, China"},{"name":"Yaoshan Laboratory, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yihang","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan 467000, China"},{"name":"Yaoshan Laboratory, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4183-834X","authenticated-orcid":false,"given":"Shiping","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan 467000, China"},{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Pingdingshan 467000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cong","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Mathematics and Statistics, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjun","family":"Han","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan 467000, China"},{"name":"Yaoshan Laboratory, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, M., Wang, S., Tang, H.P., Li, J.Y., Zhang, Z.J., Yang, B.Y., and Kuang, H.X. (2024). Buddleja officinalis Maxim.: A review of its botany, ethnopharmacology, phytochemistry, pharmacology, and therapeutic potential for ophthalmic diseases. J. Ethnopharmacol., 318.","DOI":"10.1016\/j.jep.2023.116993"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.jsps.2015.03.013","article-title":"Diabetes mellitus and oxidative stress\u2014A concise review","volume":"24","author":"Asmat","year":"2016","journal-title":"Saudi Pharm. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.cmet.2018.08.014","article-title":"The role of advanced glycation end products in aging and metabolic diseases: Bridging association and causality","volume":"28","author":"Chaudhuri","year":"2018","journal-title":"Cell Metab."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5695","DOI":"10.2174\/1381612811319320005","article-title":"Hyperglycemia-induced oxidative stress and its role in diabetes mellitus related cardiovascular diseases","volume":"19","author":"Fiorentino","year":"2013","journal-title":"Curr. Pharm. Des."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1038\/nrendo.2011.74","article-title":"AGE restriction in diabetes mellitus: A paradigm shift","volume":"7","author":"Vlassara","year":"2011","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Xie, G., Li, R., Han, Y., Zhu, Y., Wu, G., and Qin, M. (2017). Optimization of the extraction conditions for Buddleja officinalis Maxim. using response surface methodology and exploration of the optimum harvest time. Molecules, 22.","DOI":"10.3390\/molecules22111877"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3031","DOI":"10.1080\/14786419.2021.1952577","article-title":"Anti-inflammatory and antioxidant activities of chemical constituents from the flower buds of Buddleja officinalis","volume":"36","author":"Huang","year":"2022","journal-title":"Nat. Prod. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1021\/acs.jafc.5b04739","article-title":"Antioxidant activity\/capacity measurement. 1. Classification, physicochemical principles, mechanisms, and electron transfer (ET)-based assays","volume":"64","author":"Apak","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"244","DOI":"10.3736\/jcim20100308","article-title":"Effects of eye drops of Buddleja officinalis Maxim. extract on lacrimal gland cell apoptosis in castrated rats with dry eye","volume":"8","author":"Peng","year":"2010","journal-title":"J. Chin. Integr. Med."},{"key":"ref_10","first-page":"1359","article-title":"Effect of Buddleja officinalis eye drops to inflammatory factors of lacrimal gland cells of castrated male rabbit with dry eye","volume":"18","author":"Peng","year":"2018","journal-title":"Int. Eye Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1142\/S0192415X1000807X","article-title":"Anti-inflammatory effect of Buddleja officinalis on vascular inflammation in human umbilical vein endothelial cells","volume":"38","author":"Lee","year":"2010","journal-title":"Am. J. Chin. Med."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Areesanan, A., Wasilewicz, A., Kirchweger, B., Nicolay, S., Zimmermann-Klemd, A., Grienke, U., Rollinger, J.M., and Gr\u00fcndemann, C. (2024). Pharmacological in vitro profiling of Buddleja officinalis flower extracts in the context of dry eye disease. Biomed. Pharmacother., 181.","DOI":"10.1016\/j.biopha.2024.117685"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Rutkowska, M., Kolodziejczyk-Czepas, J., and Olszewska, M.A. (2021). The Effects of Sorbus aucuparia L. Fruit Extracts on Oxidative\/Nitrative Modifications of Human Fibrinogen, Impact on Enzymatic Properties of Thrombin, and Hyaluronidase Activity In Vitro. Antioxidants, 10.","DOI":"10.3390\/antiox10122009"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1080\/13880200701545018","article-title":"Inhibition of hyaluronidase activity by Vitis rotundifolia. (Muscadine) berry seeds and skins","volume":"45","author":"Bralley","year":"2007","journal-title":"Pharm. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1080\/14756366.2022.2061966","article-title":"Paving the way towards effective plant-based inhibitors of hyaluronidase and tyrosinase: A critical review on a structure\u2013activity relationship","volume":"37","author":"Graczyk","year":"2022","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1631\/jzus.B0820185","article-title":"Anti-allergic effects of ethanol extracts from brown seaweeds","volume":"10","author":"Samee","year":"2009","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_17","first-page":"200","article-title":"Optimization of ultrasonic-assisted extraction for total flavonoids from Ginkgo biloba leaves and its scavenging capacity on hydroxyl free radicals","volume":"41","author":"Yan","year":"2020","journal-title":"Sci. Technol. Food Ind."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1021\/acs.jnatprod.4c00847","article-title":"Combining the strengths of MS and NMR in biochemometrics: A case study on Buddleja officinalis","volume":"88","author":"Wasilewicz","year":"2025","journal-title":"J. Nat. Prod."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/10\/1706\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T04:32:08Z","timestamp":1779251528000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/10\/1706"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,18]]},"references-count":18,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["molecules31101706"],"URL":"https:\/\/doi.org\/10.3390\/molecules31101706","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,18]]}}}