{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T01:03:03Z","timestamp":1779670983831,"version":"3.53.1"},"reference-count":34,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Background: An original pre-column derivatisation strategy combining liquid chromatography, supported by gas chromatography, was developed for the determination of malondialdehyde (MDA), formaldehyde (FA), and 4-hydroxynonenal (4-HNE) in selected plant oils and model edible animal tissues (i.e., muscle, adipose tissue, liver, and brain). Methods: In oils, direct derivatisation with 2,4-dinitrophenylhydrazine (DNPH) was applied to quantify the target aldehydes (as hydrazones) without prior saponification. In the analysed animal tissue samples, MDA and FA were released by saponification and subsequently derivatised with DNPH, whereas 4-HNE was extracted from these samples and subsequently derivatised with DNPH. Derivatised aldehydes were quantified using C18 ultra-high performance liquid chromatography (C18-UHPLC) with photodiode array detection (DAD) under binary-gradient elution conditions, supported by gas chromatography (GC) with flame ionisation detection (FID). Results: The combination of the original binary gradient elution programme, selective DAD, and a high-performance C18 column (150 mm, 1.6 \u00b5m particle size) resulted in excellent baseline stability, good linearity, and satisfactory repeatability and specificity in the determination of MDA, FA, and 4-HNE. C18-UHPLC\u2013DAD enabled satisfactory separation of MDA, FA and 4-HNE hydrazones from endogenous matrix components in solutions of processed oils and animal tissues, while the addition of acetonitrile to these sample solutions further reduced background interference. C18-UPLC-DAD provided satisfactory symmetrical peak shapes, peak purities, and recoveries of MDA, FA, and 4-HNE in analysed plant oils and ovine tissues, compared with GC\u2013FID. Compared with GC\u2013FID, C18-UHPLC-DAD provided superior resolution of derivatised aldehydes in matrices of analysed biological samples. Conclusions: The determination of lipid peroxidation biomarkers in oils and animal tissues using our novel C18-UHPLC-DAD method may contribute to the optimisation of breeding practices, helping to minimise animal stress and enhance the health-promoting properties of food products.<\/jats:p>","DOI":"10.3390\/molecules31111800","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:30:33Z","timestamp":1779669033000},"page":"1800","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive Determination of Lipid Peroxidation Biomarkers in Ovine Tissues and Plant Oils by C18-UHPLC-DAD and GC\u2013FID"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7475-5340","authenticated-orcid":false,"given":"Marian","family":"Czauderna","sequence":"first","affiliation":[{"name":"The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Street, 05-110 Jab\u0142onna, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2003-8642","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Bia\u0142ek","sequence":"additional","affiliation":[{"name":"The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Street, 05-110 Jab\u0142onna, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0319-0770","authenticated-orcid":false,"given":"Wiktoria","family":"Wojtak","sequence":"additional","affiliation":[{"name":"The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Street, 05-110 Jab\u0142onna, Poland"},{"name":"Institute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8 Street, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7879-300X","authenticated-orcid":false,"given":"Agnieszka","family":"Bia\u0142ek","sequence":"additional","affiliation":[{"name":"The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Street, 05-110 Jab\u0142onna, Poland"},{"name":"School of Health and Medical Sciences, VIZJA University, Okopowa 59 Street, 01-043 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeriia","family":"Fesenko","sequence":"additional","affiliation":[{"name":"The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Street, 05-110 Jab\u0142onna, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1042\/EBC20200067","article-title":"Mammalian lipids: Structure, synthesis and function","volume":"65","author":"Cockcroft","year":"2021","journal-title":"Essays Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"108941","DOI":"10.1016\/j.abb.2021.108941","article-title":"Lipid peroxidation as measured by chromatographic determination of malondialdehyde. 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