{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:12:58Z","timestamp":1785420778148,"version":"3.56.0"},"reference-count":22,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007224","name":"National Foundation for Science and Technology Development","doi-asserted-by":"publisher","award":["106.02-2021.43"],"award-info":[{"award-number":["106.02-2021.43"]}],"id":[{"id":"10.13039\/100007224","id-type":"DOI","asserted-by":"publisher"}]},{"award":["106.02-2021.43"],"award-info":[{"award-number":["106.02-2021.43"]}],"id":[{"id":"https:\/\/ror.org\/04rw64z44","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study aimed to investigate the anti-allergic potential of the ethyl acetate fraction (EtOAc) from Phyllanthus amarus using an ovalbumin (OVA)-induced allergic mouse model and in vitro RBL-2H3 mast cell assays. The EtOAc fraction was characterized by high total phenolic and flavonoid contents, reaching 261 \u00b1 18 mg GAE\/g EtOAc fraction and 86 \u00b1 7 mg QE\/g EtOAc fraction, respectively. It was found that EtOAc fraction significantly reduced histamine release up to 43.3% and suppressed reactive oxygen species (ROS) production from Fc\u025bRI-mediated mast cell activation at a concentration treatment of 200 \u00b5g\/mL. Furthermore, EtOAc fraction decreased interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-\u03b1) release up to 94.2 pg\/mL and 195.6 pg\/mL, respectively. In the OVA-induced allergic mouse model, EtOAc fraction treatment markedly lowered sneezing frequency and serum IgE and histamine levels by approximately 50% at a dose treatment of 50 mg\/kg. In addition, histopathological analysis revealed that the EtOAc fraction significantly alleviated inflammatory cell infiltration, particularly eosinophils, in lung tissue. Accordingly, the results highlight the potential of EtOAc fraction from P. amarus as a natural candidate for managing allergic diseases.<\/jats:p>","DOI":"10.3390\/molecules31111806","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:30:33Z","timestamp":1779669033000},"page":"1806","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Role of Ethyl Acetate Fraction from Phyllanthus amarus in Down-Regulation of Allergic Inflammatory Responses"],"prefix":"10.3390","volume":"31","author":[{"given":"Thanh Sang","family":"Vo","sequence":"first","affiliation":[{"name":"Center for Hi-Tech Development, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam"},{"name":"Interdisciplinary Science Institute, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Ngoc My","family":"Vo","sequence":"additional","affiliation":[{"name":"Faculty of Medical Laboratory, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1108-3521","authenticated-orcid":false,"given":"Hoang Dung","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Institute of Life Sciences, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7177-6557","authenticated-orcid":false,"given":"Dai-Nghiep","family":"Ngo","sequence":"additional","affiliation":[{"name":"Faculty of Biology-Biotechnology, University of Science, Ho Chi Minh City 700000, Vietnam"},{"name":"Vietnam National University, Ho Chi Minh City 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quang Vinh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot City 630000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4025-1694","authenticated-orcid":false,"given":"Dai-Hung","family":"Ngo","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Thu Dau Mot University, Ho Chi Minh City 750000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"681","DOI":"10.3897\/pharmacia.69.e87013","article-title":"A review of the Phyllanthus genus plants: Their phytochemistry, traditional uses, and potential inhibition of xanthine oxidase","volume":"69","author":"Husnunnisa","year":"2022","journal-title":"Pharmacia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s13237-022-00409-z","article-title":"An insight into the potent medicinal plant Phyllanthus amarus Schum. and Thonn","volume":"65","author":"Ghosh","year":"2022","journal-title":"Nucleus"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Nisar, M.F., He, J., Ahmed, A., Yang, Y., Li, M., and Wan, C. (2018). Chemical components and biological activities of the genus Phyllanthus: A review of the recent literature. Molecules, 23.","DOI":"10.3390\/molecules23102567"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Abd Rani, N.Z., Lam, K.W., Jalil, J., Mohamad, H.F., Mat Ali, M.S., and Husain, K. (2021). Mechanistic studies of the antiallergic activity of Phyllanthus amarus Schum. & Thonn. and its compounds. Molecules, 26.","DOI":"10.3390\/molecules26030695"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"196","DOI":"10.46542\/pe.2023.234.196202","article-title":"Investigating the anti-allergic activity of Phyllanthus niruri via MALT1 protease inhibition: An in silico approach","volume":"23","author":"Marhaeny","year":"2023","journal-title":"Pharm. 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