{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:21:31Z","timestamp":1785424891440,"version":"3.56.0"},"reference-count":40,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chinese Academy of Medical Sciences (CAMS) Science and Technology Innovation Project for Medicine and Health","award":["2021-I2M-1-031"],"award-info":[{"award-number":["2021-I2M-1-031"]}]},{"award":["2021-I2M-1-031"],"award-info":[{"award-number":["2021-I2M-1-031"]}],"id":[{"id":"https:\/\/ror.org\/02drdmm93","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Yunnan Science and Technology Talent and Platform Program","award":["202405AF140073"],"award-info":[{"award-number":["202405AF140073"]}]},{"name":"Major Science and Technology Special Project of Yunnan Province","award":["202402AA310041"],"award-info":[{"award-number":["202402AA310041"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Cisplatin-induced acute kidney injury (AKI) poses a significant clinical challenge lacking specific therapeutic drugs. Arundina graminifolia, a traditional Dai medicine, exhibits notable renoprotective effects; however, its in vivo pharmacodynamic material basis and molecular mechanisms remain unclear. This study aimed to explore its mechanisms against AKI from the perspective of authentic kidney-migrating components. A cisplatin-induced mouse AKI model was established to evaluate the renoprotective effects of the A. graminifolia extract (BYJ) via biochemical markers and histopathology. UPLC-Q-TOF-MS\/MS was employed to comparatively analyze the blood and kidney-migrating components between normal and AKI mice. Network pharmacology and molecular docking were subsequently applied to predict and validate the core signaling pathways based on the specific components detected in the injured kidneys. Results showed that BYJ administration significantly ameliorated renal dysfunction, restored antioxidant capacity, and alleviated tubular necrosis. MS analysis identified 93 chemical components in vitro. In vivo tracking revealed a \u201cpathological targeted recruitment\u201d characteristic: only 6 prototype components entered normal kidneys, whereas 16 prototypes penetrated the AKI kidneys, highly enriched in lipophilic flavonoid aglycones such as kaempferol and apigenin. Network pharmacology predicted that these targeted components could potentially interact with 124 key targets (including AKT1, PIK3CA, and EGFR) to putatively exert anti-apoptotic and anti-inflammatory effects via the PI3K-Akt, TNF, and MAPK pathways. Molecular docking confirmed excellent binding affinities between these aglycones and core target proteins (e.g., kaempferol with PIK3CA at \u22128.9 kcal\/mol). Based on actual in vivo distribution, this study reveals the specific accumulation of polyhydroxy flavonoid aglycones in injured kidneys, providing a reliable scientific basis for defining the pharmacodynamic substances of A. graminifolia.<\/jats:p>","DOI":"10.3390\/molecules31111951","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T11:52:30Z","timestamp":1780573950000},"page":"1951","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Arundina graminifolia Ameliorates Cisplatin-Induced Acute Kidney Injury via Pathological Targeted Recruitment of Flavonoid Aglycones: A Study Integrating Serum\/Kidney Pharmacochemistry and Network Pharmacology"],"prefix":"10.3390","volume":"31","author":[{"given":"Meijia","family":"Chen","sequence":"first","affiliation":[{"name":"Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Xishuangbanna 666100, China"},{"name":"Yunnan Key Laboratory of Southern Medicine Utilization, Xishuangbanna 666100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5065-325X","authenticated-orcid":false,"given":"Yu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianglong","family":"Chen","sequence":"additional","affiliation":[{"name":"Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Xishuangbanna 666100, China"},{"name":"Yunnan Key Laboratory of Southern Medicine Utilization, Xishuangbanna 666100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rujie","family":"Zhou","sequence":"additional","affiliation":[{"name":"Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Xishuangbanna 666100, China"},{"name":"Yunnan Key Laboratory of Southern Medicine Utilization, Xishuangbanna 666100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2393-5269","authenticated-orcid":false,"given":"Guang","family":"Li","sequence":"additional","affiliation":[{"name":"Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Xishuangbanna 666100, China"},{"name":"Yunnan Key Laboratory of Southern Medicine Utilization, Xishuangbanna 666100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1038\/s41572-021-00284-z","article-title":"Acute Kidney Injury","volume":"7","author":"Kellum","year":"2021","journal-title":"Nat. Rev. Dis. Prim."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/s41581-018-0052-0","article-title":"Global Epidemiology and Outcomes of Acute Kidney Injury","volume":"14","author":"Hoste","year":"2018","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/s41581-022-00631-7","article-title":"Cisplatin Nephrotoxicity: New Insights and Therapeutic Implications","volume":"19","author":"Tang","year":"2022","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1093\/toxres\/tfac019","article-title":"Mechanism of Kidney Injury Induced by Cisplatin","volume":"11","author":"Huang","year":"2022","journal-title":"Toxicol. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1681\/ASN.2015070740","article-title":"For The Acute Dialysis Quality Initiative Xiii Working Group Cellular and Molecular Mechanisms of AKI","volume":"27","author":"Agarwal","year":"2016","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"114143","DOI":"10.1016\/j.jep.2021.114143","article-title":"Phytochemistry and Pharmacological Activities of Arundina graminifolia (D.Don) Hochr. And Other Common Orchidaceae Medicinal Plants","volume":"276","author":"Zhang","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1016\/j.sajb.2022.08.048","article-title":"Arundina graminifolia (D.Don) Hochr. (Orchidaceae): A Review of Its Medicinal Importance, Phytochemistry and Pharmacology Activities","volume":"150","author":"Kaur","year":"2022","journal-title":"S. Afr. J. Bot."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"141745","DOI":"10.1016\/j.molstruc.2025.141745","article-title":"Isolation, Bioactivity and Molecular Docking of Stilbene Acetylcholinesterase Inhibitors from Arundina graminifolia (D. Don) Hochr","volume":"1332","author":"Zhang","year":"2025","journal-title":"J. Mol. Struct."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1080\/102860204100016890181","article-title":"One New Benzyldihydrophenanthrene from Arundina graminifolia","volume":"7","author":"Liu","year":"2005","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.sajb.2022.07.020","article-title":"Chemical Composition, Antioxidant Activity and Bioactive Constituents of Six Native Endangered Medicinal Orchid Species from North-Eastern Himalayan Region of India","volume":"150","author":"Natta","year":"2022","journal-title":"S. Afr. J. Bot."},{"key":"ref_11","first-page":"11","article-title":"Simultaneous Determination of Podophyllotoxin, Quercetin and Kaempferol in Podophyllin by Liquid Chromatography Tandem Mass Spectrometry","volume":"16","author":"Lin","year":"2020","journal-title":"J. Food Drug Anal."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Vargas, F., Romec\u00edn, P., Garc\u00eda-Guill\u00e9n, A.I., Wangesteen, R., Vargas-Tendero, P., Paredes, M.D., Atucha, N.M., and Garc\u00eda-Esta\u00f1, J. (2018). Flavonoids in Kidney Health and Disease. Front. Physiol., 9.","DOI":"10.3389\/fphys.2018.00394"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ahmad, S., Gao, J., Wei, Y., Lu, C., Zhu, G., and Yang, F. (2022). The Transcriptome Profiling of Flavonoids and Bibenzyls Reveals Medicinal Importance of Rare Orchid Arundina graminifolia. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.923000"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"12024","DOI":"10.1088\/1757-899X\/274\/1\/012024","article-title":"Study on the Structures and Anti-Hepatic Fibrosis Activity of Stilbenoids from Arundina graminifolia (D. Don) Hochr","volume":"274","author":"Liu","year":"2017","journal-title":"IOP Conf. Ser. Mater. Sci. Eng."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/S0731-7085(99)00276-9","article-title":"Stability Study of Selected Adenosine Nucleosides Using LC and LC\/MS Analyses","volume":"22","author":"Novotny","year":"2000","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.jchromb.2011.12.006","article-title":"Development and Application of a LC\u2013MS\/MS Method to Quantify Basal Adenosine Concentration in Human Plasma from Patients Undergoing on-Pump CABG Surgery","volume":"885\u2013886","author":"Hui","year":"2012","journal-title":"J. Chromatogr. B"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/S1044-0305(03)00127-2","article-title":"Identification of Oxidation Products and Free Radicals of Tryptophan by Mass Spectrometry","volume":"14","author":"Domingues","year":"2003","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"967826","DOI":"10.1155\/2014\/967826","article-title":"Pathophysiology of Cisplatin-Induced Acute Kidney Injury","volume":"2014","author":"Ozkok","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Holditch, S.J., Brown, C.N., Lombardi, A.M., Nguyen, K.N., and Edelstein, C.L. (2019). Recent Advances in Models, Mechanisms, Biomarkers, and Interventions in Cisplatin-Induced Acute Kidney Injury. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20123011"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"967826","DOI":"10.1038\/s41598-024-67020-x","article-title":"Global Burden of Anticancer Drug-Induced Acute Kidney Injury and Tubulointerstitial Nephritis from 1967 to 2023","volume":"14","author":"Yoon","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.phymed.2018.03.006","article-title":"Quality Transitivity and Traceability System of Herbal Medicine Products Based on Quality Markers","volume":"44","author":"Liu","year":"2018","journal-title":"Phytomedicine"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"114588","DOI":"10.1016\/j.jpba.2022.114588","article-title":"Research Progress on Quality Markers of Traditional Chinese Medicine","volume":"211","author":"Zhang","year":"2022","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"iii450","DOI":"10.1093\/ndt\/gfv190.32","article-title":"Sp220carbonyl and Oxidative Stress\u2014A New Link in Pathogenesis of Tropical Aki","volume":"30","author":"Mukhopadhyay","year":"2015","journal-title":"Nephrol. Dial. Transplant."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Rashid, H., Jali, A., Akhter, M.S., and Abdi, S.A.H. (2023). Molecular Mechanisms of Oxidative Stress in Acute Kidney Injury: Targeting the Loci by Resveratrol. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25010003"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"F1501","DOI":"10.1152\/ajprenal.00195.2018","article-title":"Immune Cells and Inflammation in AKI to CKD Progression","volume":"315","author":"Sato","year":"2018","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"557661","DOI":"10.2147\/JIR.S557661","article-title":"Inflammation: The Pathological Axis of Cisplatin-Induced Renal Injury","volume":"19","author":"Tian","year":"2026","journal-title":"J. Inflamm. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"F426","DOI":"10.1152\/ajprenal.00203.2018","article-title":"Periostin Induces Kidney Fibrosis after Acute Kidney Injury via the P38 MAPK Pathway","volume":"316","author":"An","year":"2019","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"113077","DOI":"10.1016\/j.intimp.2024.113077","article-title":"RG108 Attenuates Acute Kidney Injury by Inhibiting P38 MAPK\/FOS and JNK\/JUN Pathways","volume":"142","author":"Kong","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"S17","DOI":"10.1016\/j.ekir.2023.02.038","article-title":"Wcn23-0818 Paeoniflorin Protects against Cisplatin-Induced Acute Kidney Injury through the Pi3k-Akt Pathway","volume":"8","author":"Zhang","year":"2023","journal-title":"Kidney Int. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"BSR20230873","DOI":"10.1042\/BSR20230873","article-title":"Kaempferol Improves Acute Kidney Injury via Inhibition of Macrophage Infiltration in Septic Mice","volume":"43","author":"Xu","year":"2023","journal-title":"Biosci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"8907717","DOI":"10.1155\/2024\/8907717","article-title":"Potential Nephroprotective Effect of Kaempferol: Biosynthesis, Mechanisms of Action, and Clinical Prospects","volume":"2024","author":"Alkandahri","year":"2024","journal-title":"Adv. Pharmacol. Pharm. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.freeradbiomed.2023.12.010","article-title":"Isorhamnetin Alleviates Cisplatin-Induced Acute Kidney Injury via Enhancing Fatty Acid Oxidation","volume":"212","author":"Wang","year":"2024","journal-title":"Free Radic. Biol. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"F107","DOI":"10.1152\/ajprenal.00347.2019","article-title":"TNF-\u03b1 in T Lymphocytes Attenuates Renal Injury and Fibrosis during Nephrotoxic Nephritis","volume":"318","author":"Wen","year":"2020","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.foodchem.2004.09.028","article-title":"The Effects of Different Extraction Solvents of Varying Polarities on Polyphenols of Orthosiphon Stamineus and Evaluation of the Free Radical-Scavenging Activity","volume":"93","author":"Akowuah","year":"2005","journal-title":"Food Chem."},{"key":"ref_35","first-page":"1462802","article-title":"Cisplatin-Induced Rodent Model of Kidney Injury: Characteristics and Challenges","volume":"2018","year":"2018","journal-title":"BioMed Res. Int."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1258\/la.2008.008098","article-title":"Microsatellite Analysis in Two Populations of Kunming Mice","volume":"43","author":"Shang","year":"2009","journal-title":"Lab. Anim."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"185","DOI":"10.4103\/JAPTR.JAPTR_467_23","article-title":"Evaluation of the Methanol Extract of BaiYangJie: Toxicology and Protective Effect against Acute Kidney Injury","volume":"15","author":"Li","year":"2024","journal-title":"J. Adv. Pharm. Technol. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"W32","DOI":"10.1093\/nar\/gku293","article-title":"SwissTargetPrediction: A Web Server for Target Prediction of Bioactive Small Molecules","volume":"42","author":"Gfeller","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Que, W., Chen, M., Yang, L., Zhang, B., Zhao, Z., Liu, M., Cheng, Y., and Qiu, H. (2021). A Network Pharmacology-Based Investigation on the Bioactive Ingredients and Molecular Mechanisms of Gelsemium Elegans Benth against Colorectal Cancer. BMC Complement. Med. Ther., 21.","DOI":"10.1186\/s12906-021-03273-7"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Fu, K., Li, Y., Dai, S., and Li, Y. (2023). Exploration of the Molecular Basis of Forsythia Fruit in the Prevention and Treatment of Cholestatic Liver Injury through Network Pharmacology and Molecular Docking. Nutrients, 15.","DOI":"10.3390\/nu15092065"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/11\/1951\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T11:53:36Z","timestamp":1780574016000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/11\/1951"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,4]]},"references-count":40,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["molecules31111951"],"URL":"https:\/\/doi.org\/10.3390\/molecules31111951","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,4]]}}}