{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:01:02Z","timestamp":1785416462074,"version":"3.56.0"},"reference-count":49,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["22-75-10151-P"],"award-info":[{"award-number":["22-75-10151-P"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"award":["22-75-10151-P"],"award-info":[{"award-number":["22-75-10151-P"]}],"id":[{"id":"https:\/\/ror.org\/03y2gwe85","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Hypericin (Hyp) is a naturally occurring photosensitizer (PS) exhibiting a broad spectrum of biological activities. However, its high hydrophobicity significantly limits its medical applicability. This study aimed to develop an efficient method for extracting Hyp from Hypericum perforatum L. biomass and to obtain its water-soluble micellar formulation. A protocol for Hyp isolation from the aerial parts of the plant was established, involving a preliminary defatting step using a Soxhlet apparatus, followed by ultrasonic-assisted extraction with acetone. The water-soluble formulation was prepared via the thin-film hydration method using the nonionic block copolymer Pluronic F-127. The resulting micelles demonstrated colloidal stability, with a mean hydrodynamic diameter of approximately 30 nm. A high Hyp loading efficiency was achieved, with an encapsulation efficiency (EE) of 95.4 \u00b1 2.7%, yielding a concentration of 600.6 \u00b1 16.6 \u00b5M within the micellar formulation. In vitro biological studies were performed using the murine GL261 glioblastoma cell line. The micellar Hyp formulation exhibited efficient time-dependent cellular accumulation and photoinduced cytotoxicity, with no observable dark toxicity. The proposed method enables the production of a stable Hyp formulation that retains its photosensitizing properties, thereby opening promising avenues for its application in photodynamic and sonodynamic therapies.<\/jats:p>","DOI":"10.3390\/molecules31122048","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T12:14:58Z","timestamp":1781180098000},"page":"2048","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Method for Isolating Hypericin from Hypericum perforatum and Preparing Its Micelles for Biomedical Applications"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3618-4629","authenticated-orcid":false,"given":"Dmitry","family":"Medvedev","sequence":"first","affiliation":[{"name":"Department of Chemistry and Technology of Biologically Active Compounds, Medicinal and Organic Chemistry, Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 86 Vernadsky Avenue, 119571 Moscow, Russia"},{"name":"Frumkin Institute of Physical Chemistry and Electrochemistry of Russian Academy of Sciences, 119071 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasilisa","family":"Dalinina","sequence":"additional","affiliation":[{"name":"Laboratory of Synthetic Neurotechnologies, Pirogov Russian National Medical University, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polina","family":"Golik","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technology of Biologically Active Compounds, Medicinal and Organic Chemistry, Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 86 Vernadsky Avenue, 119571 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polina","family":"Lavrinova","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technology of Biologically Active Compounds, Medicinal and Organic Chemistry, Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 86 Vernadsky Avenue, 119571 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8711-6919","authenticated-orcid":false,"given":"Ekaterina","family":"Plotnikova","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technology of Biologically Active Compounds, Medicinal and Organic Chemistry, Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 86 Vernadsky Avenue, 119571 Moscow, Russia"},{"name":"National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, P.A. Hertsen Moscow Oncology Research Institute, 125284 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8266-6337","authenticated-orcid":false,"given":"Maksim","family":"Usachev","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technology of Biologically Active Compounds, Medicinal and Organic Chemistry, Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 86 Vernadsky Avenue, 119571 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0984-3773","authenticated-orcid":false,"given":"Veronika","family":"Usatova","sequence":"additional","affiliation":[{"name":"Federal Center of Brain Research and Neurotechnologies, Federal Medical Biological Agency, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail","family":"Grin","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technology of Biologically Active Compounds, Medicinal and Organic Chemistry, Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 86 Vernadsky Avenue, 119571 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Abakumova","sequence":"additional","affiliation":[{"name":"Laboratory of Synthetic Neurotechnologies, Pirogov Russian National Medical University, 117997 Moscow, Russia"},{"name":"Federal Center of Brain Research and Neurotechnologies, Federal Medical Biological Agency, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Ostroverkhov","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technology of Biologically Active Compounds, Medicinal and Organic Chemistry, Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 86 Vernadsky Avenue, 119571 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Benzie, I.F.F., and Wachtel-Galor, S. (2011). Medical Attributes of St. John\u2019s Wort (Hypericum perforatum). Herbal Medicine: Biomolecular and Clinical Aspects, CRC Press\/Taylor & Francis. [2nd ed.].","DOI":"10.1201\/b10787"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Lowen, E., Moulton, S.E., Palombo, E.A., Kwa, F., and Zaferanloo, B. (2026). Harnessing Endophytic Fungi as a Sustainable Source of Novel Anticancer Agents: Opportunities, Challenges, and Future Directions. Molecules, 31.","DOI":"10.3390\/molecules31040693"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"558923","DOI":"10.2147\/DDDT.S558923","article-title":"Hypericin as a Photodynamic Immunomodulator: A Natural Compound for Dermatological Therapy","volume":"20","author":"Ma","year":"2026","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2478","DOI":"10.2174\/0115680266330142250224101958","article-title":"Diverse Therapeutic Potentials of Hypericin: An In-Depth Review","volume":"25","author":"Maurya","year":"2025","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ion, V., Ielciu, I., C\u00e2rje, A.-G., Muntean, D.L., Cri\u015fan, G., and P\u0103ltinean, R. (2022). Hypericum Spp.\u2014An Overview of the Extraction Methods and Analysis of Compounds. Separations, 9.","DOI":"10.3390\/separations9010017"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"104411","DOI":"10.1016\/j.pdpdt.2024.104411","article-title":"Green Synthesis and in Vitro Photodynamic Efficacy of Hypericin: Cytotoxicity Assessment on MCF-7 Breast Cancer Cells","volume":"50","author":"Priyadarshini","year":"2024","journal-title":"Photodiagn. Photodyn. Ther."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"115545","DOI":"10.1016\/j.biopha.2023.115545","article-title":"Novel Antidepressant Mechanism of Hypericin: Role of Connexin 43-Based Gap Junctions","volume":"167","author":"Wang","year":"2023","journal-title":"Biomed. Pharmacother."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Ilieva, Y., Marinov, T., Trayanov, I., Kaleva, M., Zaharieva, M.M., Yocheva, L., Kokanova-Nedialkova, Z., Najdenski, H., and Nedialkov, P. (2023). Outstanding Antibacterial Activity of Hypericum Rochelii\u2014Comparison of the Antimicrobial Effects of Extracts and Fractions from Four Hypericum Species Growing in Bulgaria with a Focus on Prenylated Phloroglucinols. Life, 13.","DOI":"10.3390\/life13020274"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/s12985-026-03087-4","article-title":"Inhibition of Dengue Virus Type 2 by Hypericin Mediated through Viral Envelope Protein Interaction","volume":"23","author":"Qi","year":"2026","journal-title":"Virol. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3803","DOI":"10.1007\/s00210-023-02915-6","article-title":"Hypericum Perforatum: A Comprehensive Review on Pharmacognosy, Preclinical Studies, Putative Molecular Mechanism, and Clinical Studies in Neurodegenerative Diseases","volume":"397","author":"Suryawanshi","year":"2024","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1007\/s43630-026-00879-z","article-title":"Targeted Photodynamic Therapy for MCF-7 Breast Cancer Using Antibody-Conjugated, Green-Synthesized Gold Nanoparticles Loaded with Hypericin: A Proof of Concept","volume":"25","author":"Mohlongo","year":"2026","journal-title":"Photochem. Photobiol. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"P\u00e9li, E.R., and Cserhalmi, D. (2026). Primary and Hepatogenous Photosensitization in Livestock: A Review of Plant-Derived Phototoxins and Veterinary Implications. Vet. Sci., 13.","DOI":"10.20944\/preprints202602.0847.v1"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Fiegler-Rudol, J., Zi\u0119ba, N., Turski, R., Misio\u0142ek, M., and Wiench, R. (2025). Hypericin-Mediated Photodynamic Therapy for Head and Neck Cancers: A Systematic Review. Biomedicines, 13.","DOI":"10.3390\/biomedicines13010181"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"103821","DOI":"10.1016\/j.pdpdt.2023.103821","article-title":"Imaging of Heterogeneity in 3D Spheroids of U87MG Glioblastoma Cells and Its Implications for Photodynamic Therapy","volume":"44","year":"2023","journal-title":"Photodiagn. Photodyn. Ther."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"96","DOI":"10.33263\/BRIAC144.096","article-title":"Use of Hypericin in Clinical Trials of Photodynamic Therapy","volume":"14","author":"Mazur","year":"2024","journal-title":"Biointerface Res. Appl. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.pdpdt.2017.06.003","article-title":"Sonodynamic Therapy, a Treatment Developing from Photodynamic Therapy","volume":"19","author":"Liu","year":"2017","journal-title":"Photodiagn. Photodyn. Ther."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Chen, P., Zhang, P., Shah, N.H., Cui, Y., and Wang, Y. (2023). A Comprehensive Review of Inorganic Sonosensitizers for Sonodynamic Therapy. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241512001"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5685","DOI":"10.7150\/thno.130498","article-title":"Sensitization Strategy for Sonodynamic Therapy","volume":"16","author":"Li","year":"2026","journal-title":"Theranostics"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6002","DOI":"10.1039\/D5RA03591K","article-title":"Nanobiotechnology-Driven Advances in Sonodynamic Therapy: Unlocking the Potential of Sonosensitizers in Clinical Translation","volume":"16","author":"Zeng","year":"2026","journal-title":"RSC Adv."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2204365","DOI":"10.1002\/advs.202204365","article-title":"Emerging Sonodynamic Therapy-Based Nanomedicines for Cancer Immunotherapy","volume":"10","author":"Yang","year":"2023","journal-title":"Adv. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Foglietta, F., Canaparo, R., Cossari, S., Panzanelli, P., Dosio, F., and Serpe, L. (2022). Ultrasound Triggers Hypericin Activation Leading to Multifaceted Anticancer Activity. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14051102"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"118041","DOI":"10.1016\/j.biopha.2025.118041","article-title":"Glioblastoma Targeting by Water-Soluble Hypericin Derivate HHL-PVP and Photodynamic Tumour Killing","volume":"186","author":"Vejzovic","year":"2025","journal-title":"Biomed. Pharmacother."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Jernej, L., Gschwendtner, S., Kubin, A., Wightman, L., and Plaetzer, K. (2025). Hypericin-Mediated Photodynamic Inactivation Against the Plant Pathogen Clavibacter Michiganensis: Preventative Seed Decontamination Enhanced by Potassium Iodide. Microorganisms, 13.","DOI":"10.3390\/microorganisms13102360"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"112785","DOI":"10.1016\/j.jphotobiol.2023.112785","article-title":"Effective Transport of Aggregated Hypericin Encapsulated in SBA-15 Nanoporous Silica Particles for Photodynamic Therapy of Cancer Cells","volume":"247","author":"Benziane","year":"2023","journal-title":"J. Photochem. Photobiol. B"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.pdpdt.2018.12.013","article-title":"Benefits of Hypericin Transport and Delivery by Low- and High-Density Lipoproteins to Cancer Cells: From in Vitro to Ex Ovo","volume":"25","author":"Lenkavska","year":"2019","journal-title":"Photodiagn. Photodyn. Ther."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"121195","DOI":"10.1016\/j.ijpharm.2021.121195","article-title":"Thermosensitive Liposomes Encapsulating Hypericin: Characterization and Photodynamic Efficiency","volume":"609","author":"Alawak","year":"2021","journal-title":"Int. J. Pharm."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Devarajan, P.V., Dyawanapelly, S., and Junnuthula, V. (2026). Stimuli-Responsive Liposomes for Cancer Therapy. Advances in Stimuli-Responsive Nanosystems for Cancer Therapy, Springer Nature.","DOI":"10.1007\/978-981-95-2744-1"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.jphotobiol.2018.11.019","article-title":"Hypericin Photodynamic Activity in DPPC Liposome. PART I: Biomimetism of Loading, Location, Interactions and Thermodynamic Properties","volume":"190","author":"Vilsinski","year":"2019","journal-title":"J. Photochem. Photobiol. B"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"112782","DOI":"10.1016\/j.jphotobiol.2023.112782","article-title":"Advanced theranostic nanoplatforms for hypericin delivery in the cancer treatment","volume":"247","author":"Balbinot","year":"2023","journal-title":"J. Photochem. Photobiol. B."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.31557\/APJCP.2022.23.5.1741","article-title":"Effects of Hypericin Encapsulated on Pluronic F127 Photodynamic Therapy against Triple Negative Breast Cancer","volume":"23","author":"Meirelles","year":"2022","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wang, Q., Atluri, K., Tiwari, A.K., and Babu, R.J. (2023). Exploring the Application of Micellar Drug Delivery Systems in Cancer Nanomedicine. Pharmaceuticals, 16.","DOI":"10.3390\/ph16030433"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An Open-Source Platform for Biological-Image Analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/15422119.2020.1797792","article-title":"Hypericin: Source, Determination, Separation, and Properties","volume":"51","author":"Zhang","year":"2020","journal-title":"Sep. Purif. Rev."},{"key":"ref_34","first-page":"81","article-title":"Hypericin: Chemical Synthesis and Biosynthesis","volume":"12","author":"Huang","year":"2014","journal-title":"Chin. J. Nat. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1039\/c9pp00118b","article-title":"Influence of the Electron Donor Properties of Hypericin on Its Sensitizing Ability in DSSCs","volume":"18","author":"Zobenica","year":"2019","journal-title":"Photochem. Photobiol. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1002\/jssc.200800700","article-title":"Rapid and Efficient Purification of Naphthodianthrones from St. John\u2019s Wort Extract by Using Liquid\u2013Liquid Extraction and SEC","volume":"32","author":"Karioti","year":"2009","journal-title":"J. Sep. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1080\/03639045.2017.1313857","article-title":"Improved Solubility and Oral Bioavailability of Apigenin via Soluplus\/Pluronic F127 Binary Mixed Micelles System","volume":"43","author":"Zhang","year":"2017","journal-title":"Drug Dev. Ind. Pharm."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3241","DOI":"10.1007\/s00210-024-03582-x","article-title":"Next-Generation Cancer Nanotherapeutics: Pluronic\u00ae F127 Based Mixed Micelles for Enhanced Drug Delivery","volume":"398","author":"Bhalodi","year":"2025","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Liu, S., Sun, M., and Fan, Z. (2025). Ultrasonic-Responsive Pluronic P105\/F127 Nanogels for Overcoming Multidrug Resistance in Cancer. Gels, 11.","DOI":"10.3390\/gels11110878"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.2174\/138945011795906615","article-title":"PEO-PPO Block Copolymers for Passive Micellar Targeting and Overcoming Multidrug Resistance in Cancer Therapy","volume":"12","author":"Sosnik","year":"2011","journal-title":"Curr. Drug Targets"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"104718","DOI":"10.1016\/j.jddst.2023.104718","article-title":"Enhanced activity of a pluronic F127 formulated Pin1 inhibitor for ovarian cancer therapy","volume":"87","author":"Saorin","year":"2023","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Jend\u017eelovsk\u00e1, Z., Jend\u017eelovsk\u00fd, R., Kuch\u00e1rov\u00e1, B., and Fedorocko, P. (2016). Hypericin in the Light and in the Dark: Two Sides of the Same Coin. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00560"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1021\/acsomega.4c08553","article-title":"Effects of Redox Status on Immediate Hypericin-Mediated Photodynamic Therapy in Human Glioblastoma T98G Cell Line","volume":"10","author":"Cardinali","year":"2025","journal-title":"ACS Omega"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1021\/acsapm.0c00114","article-title":"Hypericin Delivery System Based on P84 Copolymeric Micelles Linked with N -(3-Aminopropyl)-2-Pyrrolidone for Melanoma-Targeted Photodynamic Therapy","volume":"2","author":"Braga","year":"2020","journal-title":"ACS Appl. Polym. Mater."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Mischkulnig, M., Reichert, D., Wightman, L., Roth, V., H\u00f6lz, M., K\u00f6rner, L.I., Kiesel, B., Vejzovic, D., Giardina, G.A., and Erkkilae, M.T. (2024). Detection of a Water-Soluble Hypericin Formulation in Glioblastoma Tissue with Fluorescence Lifetime and Intensity Using a Dual-Tap CMOS Camera System. Diagnostics, 14.","DOI":"10.3390\/diagnostics14212423"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"119173","DOI":"10.1016\/j.saa.2020.119173","article-title":"Photophysical Characterization of Hypericin-Loaded in Micellar, Liposomal and Copolymer-Lipid Nanostructures Based F127 and DPPC Liposomes","volume":"248","author":"Caetano","year":"2021","journal-title":"Spectrochim. Acta Part A"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"104043","DOI":"10.1016\/j.jddst.2022.104043","article-title":"Poloxamers-Based Nanomicelles as Delivery Vehicles of Hypericin for Hepatic Photodynamic Therapy","volume":"79","author":"Martins","year":"2023","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Inglut, C.T., Sorrin, A.J., Kuruppu, T., Vig, S., Cicalo, J., Ahmad, H., and Huang, H.-C. (2020). Immunological and Toxicological Considerations for the Design of Liposomes. Nanomaterials, 10.","DOI":"10.3390\/nano10020190"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"102046","DOI":"10.1016\/j.jddst.2020.102046","article-title":"Pharmaceutical Assessment of Polyvinylpyrrolidone (PVP): As Excipient from Conventional to Controlled Delivery Systems with a Spotlight on COVID-19 Inhibition","volume":"60","author":"Kurakula","year":"2020","journal-title":"J. Drug Deliv. Sci. Technol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/12\/2048\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T12:17:26Z","timestamp":1781180246000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/12\/2048"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,11]]},"references-count":49,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["molecules31122048"],"URL":"https:\/\/doi.org\/10.3390\/molecules31122048","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,11]]}}}