{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T14:50:42Z","timestamp":1787928642881,"version":"build-2784847793"},"reference-count":57,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Unit iNOVA4Health","award":["UID\/4462\/2025"],"award-info":[{"award-number":["UID\/4462\/2025"]}]},{"name":"Research Unit iNOVA4Health","award":["UID\/PRR\/4462\/2025"],"award-info":[{"award-number":["UID\/PRR\/4462\/2025"]}]},{"name":"Research Unit iNOVA4Health","award":["UID\/PRR2\/4462\/2025"],"award-info":[{"award-number":["UID\/PRR2\/4462\/2025"]}]},{"name":"Uni\u00e3o Europeia\u2014NextGenerationEU"},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0087\/2020"],"award-info":[{"award-number":["LA\/P\/0087\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["2022.08844.PTDC"],"award-info":[{"award-number":["2022.08844.PTDC"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"award":["LA\/P\/0087\/2020"],"award-info":[{"award-number":["LA\/P\/0087\/2020"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2022.08844.PTDC"],"award-info":[{"award-number":["2022.08844.PTDC"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Ergosterol (ERG) is a bioactive sterol found in fungal cell membranes with reported cholesterol-lowering, antioxidant, and antitumor properties. Supercritical Fluid Extraction (SFE) conditions were optimized for Lentinula edodes (shiitake) using Response Surface Methodology and subsequently applied to Pleurotus ostreatus (oyster). Optimized SFE (690 bar, 69.8 \u00b0C, no co-solvent) produced significantly more concentrated ERG extracts than Soxhlet extraction for both species\u2014280.57 \u00b1 10.80 mgERG\/gextract for shiitake and 95.87 \u00b1 7.18 mgERG\/gextract for oyster\u2014corresponding to a 107% and 65% increase, respectively. Three biomass pre-treatments\u2014ultrasound, microwave, and enzymatic\u2014were evaluated in combination with SFE. Enzymatic pre-treatment with chitinase significantly improved ERG concentration: 337.53 \u00b1 23.12 mgERG\/gextract for shiitake. These results obtained after analysis of samples by GC-MS demonstrate that high-pressure SFE combined with chitinase pre-treatment is an effective strategy for producing ERG-rich extracts from fungal matrices.<\/jats:p>","DOI":"10.3390\/molecules31122067","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T09:49:51Z","timestamp":1781257791000},"page":"2067","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Supercritical Fluid Extraction of Ergosterol from Lentinula edodes and Pleurotus ostreatus: Optimization and Synergistic Effects of Biomass Pre-Treatments"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2778-0242","authenticated-orcid":false,"given":"Rita","family":"Faustino","sequence":"first","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2881-901 Oeiras, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2666-3278","authenticated-orcid":false,"given":"Ant\u00f3nio","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2881-901 Oeiras, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5016-4634","authenticated-orcid":false,"given":"Maria Ros\u00e1rio","family":"Bronze","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2881-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"FFULisboa, Faculdade de Farm\u00e1cia da Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3902-2966","authenticated-orcid":false,"given":"Naiara","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2881-901 Oeiras, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1177\/2047487313497864","article-title":"Trends in Cardiovascular Diseases and Cancer Mortality in 45 Countries from Five Continents (1980\u20132010)","volume":"21","author":"Gouvinhas","year":"2014","journal-title":"Eur. J. Prev. 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