{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:59:57Z","timestamp":1785416397920,"version":"3.56.0"},"reference-count":51,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T00:00:00Z","timestamp":1781395200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Fund for Professor of Hezhou University","award":["2024JSQD02"],"award-info":[{"award-number":["2024JSQD02"]}]},{"award":["2024JSQD02"],"award-info":[{"award-number":["2024JSQD02"]}],"id":[{"id":"https:\/\/ror.org\/04nm9ed20","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Guangxi","award":["2026GXNSFAA00640238"],"award-info":[{"award-number":["2026GXNSFAA00640238"]}]},{"name":"Natural Science Foundation of Guangxi","award":["2024GXNSFAA010380"],"award-info":[{"award-number":["2024GXNSFAA010380"]}]},{"name":"Natural Science Foundation of Guangxi","award":["2021GXNSFBA220038"],"award-info":[{"award-number":["2021GXNSFBA220038"]}]},{"name":"Natural Science Foundation of Guangxi","award":["2019GXNSFDA185003"],"award-info":[{"award-number":["2019GXNSFDA185003"]}]},{"name":"Guangxi Students\u2019 Innovation and Entrepreneurship Training Project","award":["S202511838104"],"award-info":[{"award-number":["S202511838104"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In this study, probe 1, a novel mitochondria-targeted fluorescent probe for the colorimetric and ratiometric detection of Hg2+, was developed. Upon addition of Hg2+ to the solution of 1, distinct spectral changes were observed. The absorption spectra underwent a blue shift from 510 nm to 450 nm (\u0394\u03bb = 60 nm), and the solution color changed from red to pale yellow under daylight. Concurrently, a significant blue shift occurred from 645 nm to 540 nm (\u0394\u03bb = 105 nm) in the fluorescence spectra. There were remarkable variations in the fluorescence intensity ratio of F540 nm\/F645 nm with the R\/R0 value reaching up to 824-fold, and the fluorescence color changed from red to green under a 365 nm UV lamp. Probe 1 featured a large Stokes shift of 135 nm, high sensitivity with an LOD of 25.5 nm, and excellent selectivity for Hg2+ even in the presence of other analytes. Furthermore, 1 was successfully applied for the ratiometric imaging of intracellular Hg2+ and was confirmed to localize specifically within mitochondria.<\/jats:p>","DOI":"10.3390\/molecules31122092","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T03:01:27Z","timestamp":1781492487000},"page":"2092","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mitochondria-Targeted Colorimetric and Ratiometric Fluorescent Probe for Hg2+ with Large Stokes Shift"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7484-4829","authenticated-orcid":false,"given":"Dongjian","family":"Zhu","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Health Care Food Science and Technology, College of Food and Bioengineering, Hezhou University, Hezhou 542899, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Health Care Food Science and Technology, College of Food and Bioengineering, Hezhou University, Hezhou 542899, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyan","family":"Pan","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Health Care Food Science and Technology, College of Food and Bioengineering, Hezhou University, Hezhou 542899, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Health Care Food Science and Technology, College of Food and Bioengineering, Hezhou University, Hezhou 542899, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0654-7803","authenticated-orcid":false,"given":"Aishan","family":"Ren","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Health Care Food Science and Technology, College of Food and Bioengineering, Hezhou University, Hezhou 542899, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jtemb.2012.02.004","article-title":"The toxicology of mercury and its compounds","volume":"26","author":"Syversen","year":"2012","journal-title":"J. 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