{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:17:30Z","timestamp":1785406650641,"version":"3.56.0"},"reference-count":51,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004892","name":"Agencia Espa\u00f1ola de Cooperaci\u00f3n Internacional para el Desarrollo","doi-asserted-by":"publisher","award":["2018\/SPE\/0000400192"],"award-info":[{"award-number":["2018\/SPE\/0000400192"]}],"id":[{"id":"10.13039\/501100004892","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2018\/SPE\/0000400192"],"award-info":[{"award-number":["2018\/SPE\/0000400192"]}],"id":[{"id":"https:\/\/ror.org\/00r6akf90","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Naranjilla (Solanum quitoense Lam.) is an Andean fruit crop of high economic and nutraceutical value, appreciated for its acidic-aromatic flavour and its contents of carotenoids, vitamin C and phenolic compounds. However, naranjilla production is constrained by susceptibility to pests and diseases, which has promoted the use of wild relatives from section Lasiocarpa in breeding programmes. The objective of this study was to characterise the physical, chemical, mineral and antioxidant attributes of 21 naranjilla materials developed in Ecuador and to identify elite segregants using multivariate approaches. Significant differences were observed among segregants for all evaluated variables. Antioxidant capacity, together with polyphenols and flavonoids, explained the largest proportion of total variation, whereas soluble solids and acidity were associated with organoleptic quality. Segregant P40 stood out due to its high antioxidant activity, soluble solids content and fruit weight; hence, it was identified as the most outstanding elite material. These results confirm the potential of naranjilla breeding in improving both yield and fruit quality.<\/jats:p>","DOI":"10.3390\/molecules31132217","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T01:03:56Z","timestamp":1782349436000},"page":"2217","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Characterisation of Naranjilla (Solanum quitoense Lam.) Segregants from Interspecific Crosses Within Section Lasiocarpa"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4472-4126","authenticated-orcid":false,"given":"William","family":"Viera-Arroyo","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Agropecuarias (INIAP), Av. Eloy Alfaro y Av. Amazonas, Quito 170515, Ecuador"},{"name":"Escuela Superior Polit\u00e9cnica de Chimborazo, Sede Orellana, El Coca 220001, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9274-7037","authenticated-orcid":false,"given":"Iv\u00e1n","family":"Samaniego","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Agropecuarias (INIAP), Av. Eloy Alfaro y Av. Amazonas, Quito 170515, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Salazar","sequence":"additional","affiliation":[{"name":"Food Chemistry Career, Faculty of Chemical Sciences, Central University of Ecuador, Quito 170521, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Noboa","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Agropecuarias (INIAP), Av. Eloy Alfaro y Av. Amazonas, Quito 170515, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2163-4243","authenticated-orcid":false,"given":"Wilson","family":"V\u00e1squez-Castillo","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Alimentos y Agroindustria (GIAA), Ingenier\u00eda Agroindustrial, Universidad de Las Am\u00e9ricas (UDLA), Redondel del Ciclista V\u00eda a Nay\u00f3n, Quito 170124, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Merino","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Agropecuarias (INIAP), Av. Eloy Alfaro y Av. Amazonas, Quito 170515, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s10722-020-01059-3","article-title":"Notes about lulo (Solanum quitoense Lam.): An important South American underutilized plant","volume":"68","year":"2021","journal-title":"Genet. Resour. Crop Evol."},{"key":"ref_2","first-page":"5465","article-title":"Genetic variability of the Colombian collection of lulo (Solanum quitoense Lam.) and related species of section Lasiocarpa","volume":"63","year":"2010","journal-title":"Rev. Fac. Nac. Agron. Medell\u00edn"},{"key":"ref_3","first-page":"e10386","article-title":"Nutritional and bioactive properties of Solanum quitoense Lam: Native fruit from the South American Andes","volume":"13","author":"Angeles","year":"2024","journal-title":"J. Microbiol. Biotechnol. Food Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1080\/15538362.2019.1613470","article-title":"The development of lulo plants (Solanum quitoense Lam. var. septentrionale) characterized by BBCH and landmark phenological scales","volume":"20","author":"Davenport","year":"2020","journal-title":"Int. J. Fruit Sci."},{"key":"ref_5","first-page":"57","article-title":"Morphological characterization of 39 lulo (Solanum quitoense Lam.) genotypes from the Nari\u00f1o University collection","volume":"29","author":"Riascos","year":"2012","journal-title":"Acta Agron."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"107","DOI":"10.29019\/enfoque.v10n2.425","article-title":"Use of the naranjilla (Solanum quitoense Lam.) variety INIAP Quitoense-2009 to obtain a carbonated beverage","volume":"10","author":"Gaona","year":"2019","journal-title":"Enfoque UTE"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"11890","DOI":"10.1021\/jf801515p","article-title":"Identifying carotenoids and phenolic compounds in naranjilla (Solanum quitoense Lam. var. Puyo hybrid), an Andean fruit","volume":"56","author":"Gancel","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_8","unstructured":"Reyna S\u00e1nchez, E.C. (2021). Evaluaci\u00f3n de la Resistencia a la Interacci\u00f3n Entre Meloidogyne incognita y Fusarium oxysporum en dos Especies de la Secci\u00f3n Lasiocarpa: Solanum hirtum y Solanum sp.. [Master\u2019s Thesis, Universidad de Cuenca]. Available online: https:\/\/dspace.ucuenca.edu.ec\/items\/d88e806f-4db2-4151-b9fd-4e3e2da09061."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Morillo, E., Buitr\u00f3n, J., Y\u00e1nez, D., Mournet, P., V\u00e1squez-Castillo, W., and Viteri, P. (2025). Genetic assessment in the Andean tropical fruits Solanum quitoense Lam and S. betaceum Cav.: Efforts towards a molecular breeding approach. Plants, 14.","DOI":"10.3390\/plants14060874"},{"key":"ref_10","unstructured":"Ochoa, J., Poveda, F., Manang\u00f3n, L., Clavijo, F., Eilis, M., and Allwang, J. (2026, March 23). Diversidad interespec\u00edfica como fuente de resistencia para la fusariosis de la naranjilla en Ecuador, Proceedings of the VIII Simposio Internacional de Recursos Gen\u00e9ticos de Am\u00e9rica Latina y El Caribe, Quito, Ecuador, 21\u201323 November 2011, Available online: http:\/\/repositorio.iniap.gob.ec\/handle\/41000\/3313."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Viera, W., Samaniego, I., Camacho, D., Habibi, N., Ron, L., Sediqui, N., \u00c1lvarez, J., Viteri, P., Sotomayor, A., and Merino, J. (2022). Phytochemical characterization of a tree tomato (Solanum betaceum Cav.) breeding population grown in the Inter-Andean Valley of Ecuador. Plants, 11.","DOI":"10.3390\/plants11030268"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Viera, W., Shinohara, T., Samaniego, I., Sanada, A., Terada, N., Ron, L., Su\u00e1rez-Tapia, A., and Koshio, K. (2022). Phytochemical composition and antioxidant activity of Passiflora spp. germplasm grown in Ecuador. Plants, 11.","DOI":"10.3390\/plants11030328"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Samaniego, I., Brito, B., Viera, W., Cabrera, A., Llerena, W., Kannangara, T., and Carrillo, W. (2020). Influence of the maturity stage on the phytochemical composition and the antioxidant activity of four Andean blackberry cultivars (Rubus glaucus Benth) from Ecuador. Plants, 9.","DOI":"10.3390\/plants9081027"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e20207289","DOI":"10.5935\/1806-6690.20210065","article-title":"Physical, chemical, nutritional, and antinutritional characterization of fresh peels of yellow pitaya (Selenicereus megalanthus) and red pitaya (Hylocereus costaricensis) and their flours","volume":"52","author":"Morais","year":"2021","journal-title":"Rev. Ci\u00eanc. Agron."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"89","DOI":"10.29019\/enfoqueute.v10n1.386","article-title":"Evaluaci\u00f3n f\u00edsico qu\u00edmica de fruta de pitahaya Selenicereus megalanthus en diferentes estados de desarrollo","volume":"10","author":"Sotomayor","year":"2019","journal-title":"Enfoque UTE"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Viera, W., Shinohara, T., Samaniego, I., Terada, N., Sanada, A., Ron, L., and Koshio, K. (2022). Pulp mineral content of passion fruit germplasm grown in Ecuador and its relationship with fruit quality traits. Plants, 11.","DOI":"10.3390\/plants11050697"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"190","DOI":"10.2503\/hortj.QH-150","article-title":"Physical and chemical characterization of passion fruit, focusing on the differences in juice carotenoids and sugars","volume":"94","author":"Viera","year":"2025","journal-title":"Hortic. J."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Sanmiguel, J., Andrade, V., Vargas-Tierras, Y., Samaniego, I., Paredes-Arcos, F., V\u00e1squez-Castillo, W., and Viera-Arroyo, W. (2025). Physical-chemical characterization of fruit harvested at different maturity stages of grafted yellow pitahaya (Selenicereus megalanthus Haw.). Plants, 14.","DOI":"10.3390\/plants14020178"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Viera, W., Gaona, P., Samaniego, I., Sotomayor, A., Viteri, P., Noboa, M., Merino, J., Mej\u00eda, P., and Park, C.H. (2023). Mineral content and phytochemical composition of avocado var. Hass grown using sustainable agriculture practices in Ecuador. Plants, 12.","DOI":"10.3390\/plants12091791"},{"key":"ref_20","first-page":"23","article-title":"Selecci\u00f3n de l\u00edneas promisorias de naranjilla (Solanum quitoense Lam.) para calidad de fruta","volume":"3","author":"Silva","year":"2016","journal-title":"Ecuad. Calid."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"676","DOI":"10.21273\/JASHS.139.6.676","article-title":"Variation of Carotenoids and Polyphenolics in Peach and Implications on Breeding for Modified Phytochemical Profiles","volume":"139","author":"Brown","year":"2014","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Bharti, H., Hemalatha, N., Malita, R., Sharma, V.K., Bharadwaj, R., and Babu, P. (2026). Phytochemical characterization and correlation analysis of nutritional value in sweet pepper (Capsicum annuum L.) genotypes at various growth stages. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1719537"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"88","DOI":"10.37527\/2009.59.1.013","article-title":"Chemical characterization, antioxidant properties and volatile constituents of naranjilla (Solanum quitoense Lam.) cultivated in Costa Rica","volume":"59","author":"Acosta","year":"2009","journal-title":"Arch. Latinoam. Nutr."},{"key":"ref_24","first-page":"1498","article-title":"Volatile constituents of lulo (Solanum vestissimum D.) fruit","volume":"39","author":"Duque","year":"1997","journal-title":"J. Agric. Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"157","DOI":"10.17533\/udea.vitae.12242","article-title":"Physicochemical characterization of the lulo (Solanum quitoense Lam.) castilla variety in six ripening stages","volume":"19","author":"Gaviria","year":"2012","journal-title":"Vitae"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"A\u00f1ibarro-Ortega, M., Dias, M.I., Petrovi\u0107, J., Pereira, A., Sokovi\u0107, M., Barros, L., and Pinela, J. (2025). Nutrients, Phytochemicals and In Vitro Antioxidant and Antimicrobial Activities of Lulo (Solanum quitoense Lam.) Fruit Pulp, Peel and Seeds. Foods, 14.","DOI":"10.3390\/foods14122083"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3","DOI":"10.2307\/2419045","article-title":"Artificial hybrids in Solanum sect. Lasiocarpa","volume":"14","author":"Heiser","year":"1989","journal-title":"Syst. Bot."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1590\/S1415-47572010005000016","article-title":"Genetic variation in the Solanaceae fruit bearing species lulo and tree tomato revealed by Conserved Ortholog (COSII) markers","volume":"33","author":"Lobo","year":"2010","journal-title":"Genet. Mol. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"113012","DOI":"10.1016\/j.scienta.2024.113012","article-title":"Effect of temperature, 1-methylcyclopropene and modified atmosphere packaging on the post-harvest behavior of lulo (Solanum quitoense Lam)","volume":"329","author":"Reyes","year":"2024","journal-title":"Sci. Hortic."},{"key":"ref_30","first-page":"131","article-title":"Aspectos de la fisiolog\u00eda y el cultivo del lulo (Solanum quitoense LAM.). In Colombia: Una revisi\u00f3n","volume":"13","author":"Fischer","year":"2022","journal-title":"Rev. Investig. Agrar. Ambient."},{"key":"ref_31","first-page":"1","article-title":"Physiology and biochemistry of naranjilla (Solanum quitoense Lam) fruit during postharvest and the main conservation strategies: A review","volume":"41","author":"Reyes","year":"2023","journal-title":"Agron. Colomb."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"kiaf427","DOI":"10.1093\/plphys\/kiaf427","article-title":"Genetic and transcriptomic basis of heterosis in three intra- and intersubspecific hybrid rice","volume":"199","author":"Che","year":"2025","journal-title":"Plant Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"419","DOI":"10.15446\/agron.colomb.v40n3.103082","article-title":"Characterization and classification of lulo (Solanum quitoense Lam.) fruits by ripening stage","volume":"40","year":"2022","journal-title":"Agron. Colomb."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Llerena, W., Samaniego, I., Angos, I., Brito, B., Ortiz, B., and Carrillo, W. (2019). Biocompounds content prediction in Ecuadorian fruits using a mathematical model. Foods, 8.","DOI":"10.3390\/foods8080284"},{"key":"ref_35","first-page":"145","article-title":"Evaluaci\u00f3n fisicoqu\u00edmica y antioxidante de naranjilla (Solanum quitoense Lam.) durante la maduraci\u00f3n","volume":"22","author":"Figueroa","year":"2021","journal-title":"Rev. Iberoam. Tecnol. Postcosecha"},{"key":"ref_36","first-page":"786","article-title":"Total phenolic compounds and antioxidant capacities of major fruits from Ecuador","volume":"115","author":"Vasco","year":"2009","journal-title":"Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1016\/j.foodres.2010.11.003","article-title":"Antioxidant capacity, phenolic content and vitamin C in pulp, peel and seed from 24 exotic fruits from Colombia","volume":"44","year":"2011","journal-title":"Food Res. Int."},{"key":"ref_38","first-page":"1","article-title":"Efecto del tratamiento con ozono gaseoso sobre la calidad fisicoqu\u00edmica y capacidad antioxidante de naranjilla (Solanum quitoense Lam)","volume":"19","year":"2018","journal-title":"Rev. Iberoam. Tecnol. Postcosecha"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"41","DOI":"10.15446\/rev.colomb.quim.v43n3.53615","article-title":"Total phenolic content and antioxidant activity of pulp extracts of six tropical fruits","volume":"43","author":"Moreno","year":"2014","journal-title":"Rev. Colomb. Quim."},{"key":"ref_40","first-page":"92","article-title":"Compuestos nutricionales y bioactivos de Solanum quitoense Lam (Quito quito), fruta nativa de los andes con alto potencial de nutrientes","volume":"41","author":"Arias","year":"2021","journal-title":"Tecnol. Quim."},{"key":"ref_41","first-page":"341","article-title":"Antioxidant activity and physicochemical characteristics of lulo (Solanum quitoense) fruit cultivated in Brazil","volume":"21","author":"Urbanski","year":"2022","journal-title":"Sci. Agrar. Parana."},{"key":"ref_42","first-page":"69","article-title":"Solanine and chaconine: Occurrence, properties, methods for determination","volume":"50","author":"Badowski","year":"1999","journal-title":"Rocz. Panstw. Zakl. Hig."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3375","DOI":"10.1007\/s00122-019-03431-z","article-title":"Breeding for dual-purpose wheat varieties using marker\u2013trait associations for biomass yield and quality traits","volume":"132","author":"Malik","year":"2019","journal-title":"Theor. Appl. Genet."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3454","DOI":"10.1021\/jf200439q","article-title":"Bioactivities of glycoalkaloids and their aglycones from Solanum species","volume":"59","author":"Milner","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1080\/87559129409541010","article-title":"Glycoalkaloids in solanaceae","volume":"10","author":"Maga","year":"1994","journal-title":"Food Rev. Int."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Lovric, M. (2011). Principal Component Analysis. International Encyclopedia of Statistical Science, Springer.","DOI":"10.1007\/978-3-642-04898-2"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Cao, Z., Li, J., Lei, H., Yan, M., Wang, Q., Ji, R., and Wei, Z. (2025). PCA-Driven multivariate trait integration in alfalfa breeding: A selection model for High-Yield and stable progenies. Plants, 14.","DOI":"10.3390\/plants14182906"},{"key":"ref_48","unstructured":"S\u00e1nchez, P. (2020). Caracterizaci\u00f3n de la Producci\u00f3n de Compuestos Bioactivos y An\u00e1lisis de Prote\u00ednas en Cultivos Celulares de Br\u00f3coli. [Doctoral Dissertation, Universidad de Murcia]. Available online: https:\/\/dialnet.unirioja.es\/servlet\/tesis?codigo=291197."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"155","DOI":"10.18633\/biotecnia.v20i3.712","article-title":"Extractos de hojas de plantas para conservar la calidad de la carne y los productos c\u00e1rnicos frescos","volume":"20","year":"2018","journal-title":"Revisi\u00f3n. Biotecnia"},{"key":"ref_50","unstructured":"AOAC (2000). Official Methods of Analysis, Association of Official Analytical Chemists. [17th ed.]."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.jchromb.2012.09.025","article-title":"Preparative separation of glycoalkaloids \u03b1-solanine and \u03b1-chaconine by centrifugal partition chromatography","volume":"908","author":"Lesur","year":"2012","journal-title":"J. Chromatogr. B"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/13\/2217\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T01:05:12Z","timestamp":1782349512000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/13\/2217"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,24]]},"references-count":51,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["molecules31132217"],"URL":"https:\/\/doi.org\/10.3390\/molecules31132217","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,24]]}}}