{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:29:00Z","timestamp":1785414540415,"version":"3.56.0"},"reference-count":56,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Vietnam\u2019s Ministry of Education and Training","award":["B2024-TDV-09"],"award-info":[{"award-number":["B2024-TDV-09"]}]},{"award":["B2024-TDV-09"],"award-info":[{"award-number":["B2024-TDV-09"]}],"id":[{"id":"https:\/\/ror.org\/00drv3378","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study evaluates the chemical composition and bioactivities of essential oils extracted from the leaves and twigs of Glycosmis lanceolata growing in a natural forest in Vietnam. gas chromatography\u2013mass spectrometry identified 42 and 43 constituents in the leaf and twig oils, respectively. The main compounds in the leaf oil were (E)-\u03b2-caryophyllene (10.2%), \u03b2-bisabolene (23.7%), and brevifolin (21.3%), while the twig oil was dominated by \u03b2-bisabolene (11.6%) and brevifolin (12.7%). Neither oil exhibited inhibitory effects against two beneficial bacterial strains, Bacillus subtilis and Lactobacillus fermentum. In contrast, both oils showed weak antimicrobial activity against four pathogenic bacteria\u2014Staphylococcus aureus, Salmonella enterica, Escherichia coli, and Pseudomonas aeruginosa\u2014and one yeast, Candida albicans, with IC50 values ranging from 2012 \u00b1 118 to 10,593 \u00b1 557 \u00b5g\/mL. Notably, the twig oil demonstrated pronounced anti-inflammatory activity via inhibition of nitric oxide production (IC50 = 29.7 \u00b1 2.58 \u00b5g\/mL), whereas the leaf oil showed no detectable activity within the tested concentrations. Similarly, DPPH radical scavenging assays indicated stronger antioxidant activity for the twig oil compared to the leaf oil. These findings provide new insights into the phytochemistry and bioactivities of G. lanceolata essential oils and may support further investigations into their potential applications.<\/jats:p>","DOI":"10.3390\/molecules31132246","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:49:23Z","timestamp":1782434963000},"page":"2246","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["GC-MS Profiling and In Vitro Assessment of Antimicrobial, Antioxidant, and Anti-Inflammatory Activities of Essential Oils from Wild-Growing Glycosmis lanceolata (Blume) D. Dietr. in Vietnam"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9490-4208","authenticated-orcid":false,"given":"Quang Vuong","family":"Le","sequence":"first","affiliation":[{"name":"Faculty of Biology, Vinh University, 182 Le Duan Street, Truong Vinh Ward, Nghe An 43000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3479-9469","authenticated-orcid":false,"given":"Ha Thi Thu","family":"Chu","sequence":"additional","affiliation":[{"name":"Institute of Biology, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghia Do, Hanoi 10072, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6687-9340","authenticated-orcid":false,"given":"Thuy Thi Thu","family":"Dinh","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghia Do, Hanoi 10072, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Minh Chau","family":"Dao","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Vinh University, 182 Le Duan Street, Truong Vinh Ward, Nghe An 43000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9615-5095","authenticated-orcid":false,"given":"Thi Huyen","family":"Thai","sequence":"additional","affiliation":[{"name":"Faculty of Agronomy, Hue University of Agriculture and Forestry (HUAF), 102 Phung Hung, Hue City 49000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Nghiem","family":"Vu","sequence":"additional","affiliation":[{"name":"Institute of Materials Science, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghia Do, Hanoi 10072, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5941-1004","authenticated-orcid":false,"given":"Ha Chi","family":"Vuong","sequence":"additional","affiliation":[{"name":"Newton Grammar School, 136 Ho Tung Mau, Phu Dien, Hanoi 10053, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3639-0528","authenticated-orcid":false,"given":"William N.","family":"Setzer","sequence":"additional","affiliation":[{"name":"Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA"},{"name":"Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3329\/dujps.v13i2.21887","article-title":"Alkaloid, Sterol and Triterpenoids from Glycosmis pentaphylla (Retz.) DC","volume":"13","author":"Ahmed","year":"2014","journal-title":"Dhaka Univ. J. Pharm. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1021\/np9604238","article-title":"Bioactive Amides from Glycosmis Species","volume":"59","author":"Greger","year":"1996","journal-title":"J. Nat. Prod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1248\/cpb.48.65","article-title":"Chemical Constituents of Glycosmis citrifolia (Willd.) Lindl. Structures of Four New Acridones and Three New Quinolone Alkaloids","volume":"48","author":"Ito","year":"2000","journal-title":"Chem. Pharm. Bull."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.phytol.2013.03.018","article-title":"Alkaloids from Glycosmis Cochinchinensis Twigs","volume":"6","author":"Sripisut","year":"2013","journal-title":"Phytochem. Lett."},{"key":"ref_5","first-page":"109","article-title":"Phytochemical Composition and Evaluation of Anti-Inflammatory Activity in Glycosmis pentaphylla (Retz.) DC.\u2014An Ethnobotanically Important Medicinal Plant","volume":"8","author":"Babu","year":"2020","journal-title":"Adv. Zool. Bot."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"112865","DOI":"10.1016\/j.phytochem.2021.112865","article-title":"Traditional Uses, Phytochemistry, Pharmacology, Toxicology and Formulation Aspects of Glycosmis Species: A Systematic Review","volume":"190","author":"Teja","year":"2021","journal-title":"Phytochemistry"},{"key":"ref_7","first-page":"137","article-title":"Antimicrobial, Antioxidant and Cytotoxic Effects of Methanolic Extracts of Leaves and Stems of Glycosmis pentaphylla (Retz.)","volume":"1","author":"Howlader","year":"2011","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"80","DOI":"10.7324\/JAPS.2018.81112","article-title":"Pharmacological Evaluation of Ethnomedicinal Glycosmis pentaphylla Lour. as Antidiabetic, Antioxidant and Cytotoxic Agent","volume":"8","author":"Rahman","year":"2018","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_9","first-page":"98","article-title":"The Genus Glycosmis [Rutaceae]: A Comprehensive Review on Its Phytochemical and Pharmacological Perspectives","volume":"9","author":"Yasir","year":"2019","journal-title":"Nat. Prod. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"99","DOI":"10.3329\/bpj.v29i1.87383","article-title":"Exploring the Medicinal Potential of Glycosmis cyanocarpa (Rutaceae)","volume":"29","author":"Ashrafi","year":"2026","journal-title":"Bangladesh Pharm. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"703","DOI":"10.26538\/tjnpr\/v4i10.8","article-title":"In Vitro Cytotoxic Activity of Constituents of the Aerial Parts of Glycosmis parviflora","volume":"4","author":"Nguyen","year":"2020","journal-title":"Trop. J. Nat. Prod. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"105265","DOI":"10.1016\/j.fitote.2022.105265","article-title":"Cytotoxic Constituents of Glycosmis ovoidea Collected in Vietnam","volume":"162","author":"Carcache","year":"2022","journal-title":"Fitoterapia"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Nguyen, M.T.T., Hsu, I.-C., Liu, H.-K., Lin, Y.-C., Chen, S.-R., Chang, F.-R., and Cheng, Y.-B. (2022). Components with Anti-Diabetic Activity Isolated from the Leaves and Twigs of Glycosmis pentaphylla Collected in Vietnam. Pharmaceuticals, 15.","DOI":"10.3390\/ph15121543"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"114313","DOI":"10.1016\/j.jep.2021.114313","article-title":"Ethnomedicinal Uses, Phytochemistry, Pharmacological Activities and Toxicological Profile of Glycosmis pentaphylla (Retz.) DC.: A Review","volume":"278","author":"Khandokar","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1080\/10412905.2000.9699568","article-title":"Essential Oils of Glycosmis pentaphylla (Cor.). A New Report from Assam, India","volume":"12","author":"Ahmed","year":"2000","journal-title":"J. Essent. Oil Res."},{"key":"ref_16","first-page":"50","article-title":"Chemical Fingerprint of Essential Oil Components from Fresh Leaves of Glycosmis pentaphylla (Retz.) Correa","volume":"3","author":"Prakasia","year":"2015","journal-title":"Pharma Innov."},{"key":"ref_17","first-page":"62","article-title":"Chemical Composition and Larvicidal Activity of the Essential Oil of Glycosmis pentaphylla (Retz.) Against Three Mosquito Vectors","volume":"3","author":"Vignesh","year":"2016","journal-title":"Int. J. Mosq. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1080\/14786419.2016.1226825","article-title":"Repellence of the Main Components from the Essential Oil of Glycosmis lucida Wall. Ex Huang against Two Stored Product Insects","volume":"31","author":"Guo","year":"2017","journal-title":"Nat. Prod. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"275","DOI":"10.4314\/tjpr.v13i2.17","article-title":"Gas Chromatography-Mass Spectrometric Analysis of Essential Oil of Aerial Parts of Glycosmis parviflora (Sims) Little (Rutaceae)","volume":"13","author":"Liu","year":"2014","journal-title":"Trop. J. Pharm. Res."},{"key":"ref_20","unstructured":"Hoang, D.T., Pham, H.B., Tran, D.T., and Tran, M.H. (2013, January 18). Composition of Essential Oil of Glycosmis mauritiana from Pumat National Park. Proceedings of the 5th National Conference on Ecology and Biological Resources, Hanoi, Vietnam."},{"key":"ref_21","first-page":"67","article-title":"Chemical Components of Essential Oils from the Leaves of Seven Species Belonging to Rutaceae Family from Binh Chau-Phuoc Buu Nature Reserve, Vietnam","volume":"86","author":"Van","year":"2021","journal-title":"Agric. Conspec. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1007\/s10600-025-04715-1","article-title":"Chemical Composition of Essential Oil from Glycosmis puberula var. eberhardtii and Its Herbicidal Activity","volume":"61","author":"Khoi","year":"2025","journal-title":"Chem. Nat. Compd."},{"key":"ref_23","unstructured":"Wu, Z., Raven, P.H., and Hong, D. (2008). Flora of China: Oxalidaceae Through Aceraceae, Missouri Botanical Garden Press."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.tetlet.2004.10.172","article-title":"Sulfur-Containing and Dimeric Flavanols from Glycosmis montana","volume":"46","author":"Wang","year":"2005","journal-title":"Tetrahedron Lett."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/j.phytochem.2005.02.003","article-title":"Indole and Carbazole Alkaloids from Glycosmis montana with Weak Anti-HIV and Cytotoxic Activities","volume":"66","author":"Wang","year":"2005","journal-title":"Phytochemistry"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1007\/s10600-015-1339-z","article-title":"Chemical Constituents of the Twigs and Leaves of Glycosmis montana","volume":"51","author":"Fan","year":"2015","journal-title":"Chem. Nat. Compd."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3665","DOI":"10.1080\/14786419.2024.2306175","article-title":"Three New Acridone Alkaloids from Glycosmis lanceolata (Blume) D. Dietr. and Their Cytotoxic Effects on Tumour Cell Lines","volume":"39","author":"Ito","year":"2025","journal-title":"Nat. Prod. Res."},{"key":"ref_28","first-page":"89","article-title":"Microscopical Characteristics and Leaf Oil Composition of Glycosmis lanceolata (Bl.) D. Dietr. Collected in Hai Van Pass, Danang City, Vietnam","volume":"70","author":"Vo","year":"2023","journal-title":"Vietnam J. Pharm."},{"key":"ref_29","first-page":"19","article-title":"Chemical Composition and Antioxidant Activity of Essential Oil from Glycosmis pentaphylla","volume":"4","author":"Vignesh","year":"2014","journal-title":"Int. J. Bot. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.ijfoodmicro.2004.03.022","article-title":"Essential Oils: Their Antibacterial Properties and Potential Applications in Foods\u2014A Review","volume":"94","author":"Burt","year":"2004","journal-title":"Int. J. Food Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1038\/nature01322","article-title":"Inflammation and Cancer","volume":"420","author":"Coussens","year":"2002","journal-title":"Nature"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/S0005-2728(99)00028-6","article-title":"Biological Aspects of Reactive Nitrogen Species","volume":"1411","author":"Patel","year":"1999","journal-title":"Biochim. Biophys. Acta (BBA)-Bioenerg."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/bja\/aep339","article-title":"Sepsis and the Heart","volume":"104","author":"Hunter","year":"2010","journal-title":"Br. J. Anaesth."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.indcrop.2013.02.036","article-title":"Margotia Gummifera Essential Oil as a Source of Anti-Inflammatory Drugs","volume":"47","author":"Valente","year":"2013","journal-title":"Ind. Crops Prod."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.3390\/molecules17043989","article-title":"Essential Oils in Combination and Their Antimicrobial Properties","volume":"17","author":"Juliani","year":"2012","journal-title":"Molecules"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"9252","DOI":"10.3390\/molecules15129252","article-title":"Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review","volume":"15","author":"Miguel","year":"2010","journal-title":"Molecules"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1078\/094471102321621322","article-title":"Anti-Inflammatory Activity of Linalool and Linalyl Acetate Constituents of Essential Oils","volume":"9","author":"Peana","year":"2002","journal-title":"Phytomedicine"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"6305","DOI":"10.3390\/molecules17066305","article-title":"Biological Activities of \u03b1-Pinene and \u03b2-Pinene Enantiomers","volume":"17","author":"Rivas","year":"2012","journal-title":"Molecules"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1016\/j.jss.2012.10.050","article-title":"Anti-Inflammatory Effects of Linalool in RAW 264.7 Macrophages and Lipopolysaccharide-Induced Lung Injury Model","volume":"180","author":"Huo","year":"2013","journal-title":"J. Surg. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.fct.2007.09.106","article-title":"Biological Effects of Essential Oils\u2014A Review","volume":"46","author":"Bakkali","year":"2008","journal-title":"Food Chem. Toxicol."},{"key":"ref_41","first-page":"105","article-title":"Natural Products as Antioxidants","volume":"37","author":"Maestri","year":"2006","journal-title":"Phytochem. Adv. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s11746-998-0032-9","article-title":"Free Radicals, Oxidative Stress, and Antioxidants in Human Health and Disease","volume":"75","author":"Aruoma","year":"1998","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11746-009-1483-3","article-title":"A Review of the Application and Pharmacological Properties of \u03b1-Bisabolol and \u03b1-Bisabolol-Rich Oils","volume":"87","author":"Kamatou","year":"2010","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"633","DOI":"10.3109\/10715762.2014.996146","article-title":"Advantages and Limitations of Common Testing Methods for Antioxidants","volume":"49","author":"Amorati","year":"2015","journal-title":"Free Radic. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.ejphar.2008.03.008","article-title":"Health Effects of Quercetin: From Antioxidant to Nutraceutical","volume":"585","author":"Boots","year":"2008","journal-title":"Eur. J. Pharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.fct.2009.09.007","article-title":"Chemical Composition and Antioxidant Activities of the Essential Oil and Methanol Extracts of Psammogeton Canescens","volume":"48","author":"Gholivand","year":"2010","journal-title":"Food Chem. Toxicol."},{"key":"ref_47","unstructured":"Ministry of Health (2017). Vietnamese Pharmacopoeia."},{"key":"ref_48","unstructured":"K\u00f6nig, W.A., Joulain, D., and Hochmuth, D.H. (2004). Terpenoids and Related Constituents of Essential Oils, Library of MassFinder."},{"key":"ref_49","unstructured":"Adams, R.P. (2017). Identification of Essential Oil Components by Gas Chromatography, Mass Spectrometry, Allured Publishing Corporation. [4th ed.]."},{"key":"ref_50","unstructured":"Linstrom, P.J., and Mallard, W.G. (2021). NIST Chemistry Webbook, NIST Standard Reference Database Number 69."},{"key":"ref_51","first-page":"137","article-title":"Testing of Antifungal Natural Products: Methodologies, Comparability of Results and Assay Choice","volume":"11","author":"Hadacek","year":"2000","journal-title":"Phytochem. Anal. Int. J. Plant Chem. Biochem. Tech."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.jep.2006.04.003","article-title":"Anti-Infective Potential of Natural Products: How to Develop a Stronger in Vitro \u2018Proof-of-Concept\u2019","volume":"106","author":"Cos","year":"2006","journal-title":"J. Ethnopharmacol."},{"key":"ref_53","first-page":"429","article-title":"Inhibition of Nitric Oxide (NO) Production in Lipopolysaccharide (LPS)-Activated Murine Macrophage RAW 264.7 Cells by the Norsesterterpene Peroxide, Epimuqubilin A. Mar","volume":"8","author":"Cheenpracha","year":"2010","journal-title":"Drugs"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Marques, R.V., Sestito, S.E., Bourgaud, F., Miguel, S., Cailotto, F., Reboul, P., Jouzeau, J.-Y., Rahuel-Clermont, S., Boschi-Muller, S., and Simonsen, H.T. (2022). Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages. Molecules, 27.","DOI":"10.3390\/molecules27061940"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1002\/hlca.19970800411","article-title":"Iridoid Glucosides with Free Radical Scavenging Properties from Fagraea Blumei","volume":"80","author":"Cuendet","year":"1997","journal-title":"Helv. Chim. Acta"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2080","DOI":"10.3390\/s7102080","article-title":"Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements","volume":"7","author":"Marxen","year":"2007","journal-title":"Sensors"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/13\/2246\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T04:20:46Z","timestamp":1783052446000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/13\/2246"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,25]]},"references-count":56,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["molecules31132246"],"URL":"https:\/\/doi.org\/10.3390\/molecules31132246","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,25]]}}}