{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:02:26Z","timestamp":1785402146443,"version":"3.56.0"},"reference-count":18,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004035","name":"Jordan University of Science and Technology","doi-asserted-by":"publisher","award":["20140291"],"award-info":[{"award-number":["20140291"]}],"id":[{"id":"10.13039\/501100004035","id-type":"DOI","asserted-by":"publisher"}]},{"award":["20140291"],"award-info":[{"award-number":["20140291"]}],"id":[{"id":"https:\/\/ror.org\/03y8mtb59","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Novel steroidal heterocyclic derivatives were prepared from cholesterol, stigmasterol, and diosgenin scaffolds via concise, reagent-efficient synthetic routes. Two 1,3-oxathiolan-5-one derivatives bearing cholest-5-ene (7) and stigmasta-5,22-diene (8) cores were obtained from the corresponding steroidal ketones through hydrazone formation, phenyl isothiocyanate addition, and S-selective cyclization with chloroacetic acid in refluxing toluene. An aminothiazole\u2013diosgenin hybrid (12) was independently prepared via regioselective \u03b1-bromination of an oxidized diosgenin intermediate followed by condensation with thiourea in ethanolic sodium ethoxide. The newly synthesized target compounds were characterized by FT-IR, 1H- and 13C-NMR spectroscopy, ESI mass spectrometry, and elemental (CHNS) analysis. The disc-diffusion assay against S. aureus and E. coli was retained only as a preliminary qualitative screen because no positive antibiotic control or MIC determination was included. Within this limited screen, compound 12 produced measurable 8.5\u20139.0 mm inhibition zones against both strains, supporting their prioritization for future standardized antimicrobial testing rather than establishing comparative potency.<\/jats:p>","DOI":"10.3390\/molecules31132301","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T10:41:01Z","timestamp":1782902461000},"page":"2301","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cholest-5-ene- and Stigmasta-5,22-diene-Based 1,3-Oxathiolan-5-one Lactones and an Aminothiazole\u2013Diosgenin Hybrid: Synthesis and Preliminary Antimicrobial Activity"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1455-0874","authenticated-orcid":false,"given":"Ahmad S.","family":"Barham","sequence":"first","affiliation":[{"name":"Department of Chemistry, School of Science, The University of Jordan, Amman 11942, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5308-5712","authenticated-orcid":false,"given":"Khaled Q.","family":"Shawakfeh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8560-1395","authenticated-orcid":false,"given":"Ali","family":"Elrashidi","sequence":"additional","affiliation":[{"name":"Electrical Engineering Department, College of Engineering, University of Business and Technology, Jeddah 23435, Saudi Arabia"},{"name":"Engineering Mathematics Department, Faculty of Engineering, Alexandria University, Alexandria 21544, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0672-0287","authenticated-orcid":false,"given":"Sameer Y.","family":"Jaradat","sequence":"additional","affiliation":[{"name":"Basic Sciences Department, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1021\/jm00332a027","article-title":"Steroidal Heterocycles. 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(1959). Steroids, Reinhold Publishing Corporation."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1016\/j.ejmech.2004.07.005","article-title":"Synthesis and Antifungal Activity of Cholesterol-Hydrazone Derivatives","volume":"39","author":"Loncle","year":"2004","journal-title":"Eur. J. Med. 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