{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:10:46Z","timestamp":1785431446519,"version":"3.56.0"},"reference-count":70,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Medicine and Pharmacy of Craiova"},{"id":[{"id":"https:\/\/ror.org\/031d5vw30","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Diabetes mellitus represents a major global health burden, necessitating the development of safer and more effective therapeutic alternatives. Medicinal plants have gained increasing attention due to their bioactive compounds with potential hypoglycemic and antioxidant effects. The present study aimed to investigate the physicochemical characteristics, phytochemical composition, antioxidant capacity, and toxicological profile of hydroalcoholic tinctures obtained from Agrimonia pilosa Ledeb., Calendula arvensis L., and Polygonum hydropiper L. The tinctures were prepared by simple percolation using 70% ethanol and evaluated according to pharmacopoeial standards, including organoleptic properties, relative density, refractive index, alcohol content, and purity parameters. Phytochemical analysis was performed using thin-layer chromatography and spectrophotometric methods, highlighting the presence of flavonoids and polyphenolcarboxylic acids, with several bands showing chromatographic and spectral similarities to chlorogenic and caffeic acid standards. Antioxidant activity was assessed through total polyphenol and flavonoid content, with Polygonum hydropiper exhibiting the highest values. The hypoglycemic effect was evaluated using the oral glucose tolerance test in normoglycemic mice, demonstrating significant reductions in blood glucose levels, particularly for Agrimonia pilosa at higher doses. Acute toxicity studies indicated a low toxicity profile, with no mortality observed even at high doses (up to 9 g\/kg body weight), corresponding to GHS category 5. However, subacute toxicity assessment revealed species-dependent effects, ranging from minimal hepatic changes for Calendula arvensis to moderate hepatotoxicity for Polygonum hydropiper and more pronounced hepatic, renal, and pancreatic alterations for Agrimonia pilosa. These findings suggest that the investigated tinctures possess significant hypoglycemic and antioxidant potential, with generally favorable safety profiles following acute administration. Nevertheless, prolonged use may induce organ-specific toxicity, highlighting the need for further pharmacological and clinical investigations to establish their therapeutic applicability and safety in diabetes management.<\/jats:p>","DOI":"10.3390\/molecules31132316","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T16:50:33Z","timestamp":1782924633000},"page":"2316","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Physicochemical, Phytochemical, and Toxicological Assessment of Agrimonia pilosa, Calendula arvensis, and Polygonum hydropiper Tinctures with Hypoglycemic Potential"],"prefix":"10.3390","volume":"31","author":[{"given":"Roxana","family":"Kostici","sequence":"first","affiliation":[{"name":"Department of Toxicology, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adina Maria","family":"Kamal","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana-Maria","family":"Trasca","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Vladulescu","sequence":"additional","affiliation":[{"name":"Department of Biology and Environmental Engineering, Faculty of Horticulture, University of Craiova, 200585 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Maria","family":"Varut","sequence":"additional","affiliation":[{"name":"Research Methodology Department, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pluta Ion","family":"Dorin","sequence":"additional","affiliation":[{"name":"Faculty of Medical and Behavioral Sciences, Constantin Br\u00e2ncu\u0219i University of T\u00e2rgu Jiu, 210185 T\u00e2rgu Jiu, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"C\u00eer\u021b\u00een\u0103","sequence":"additional","affiliation":[{"name":"Faculty of Medical and Behavioral Sciences, Constantin Br\u00e2ncu\u0219i University of T\u00e2rgu Jiu, 210185 T\u00e2rgu Jiu, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Stoica","sequence":"additional","affiliation":[{"name":"Department of Intensive Care and Anesthesia, Emergency County Hospital, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Pura","sequence":"additional","affiliation":[{"name":"Department of Medical Devices and Pharmaceutical Practice, Iuliu Ha\u021bieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romeo","family":"Popa","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihaela","family":"Popescu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pirscoveanu Denisa Floriana","family":"Vasilica","sequence":"additional","affiliation":[{"name":"Department of Neurology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (1999). Definition, Diagnosis and Classification of Diabetes Mellitus and Its Complications. Part 1: Diagnosis and Classification of Diabetes Mellitus; WHO\/NCD\/NCS\/99.2, World Health Organization."},{"key":"ref_2","unstructured":"World Health Organization (1985). Diabetes Mellitus: Report of a WHO Study Group, World Health Organization."},{"key":"ref_3","unstructured":"World Health Organization (2006). Definition and Diagnosis of Diabetes Mellitus and Intermediate Hyperglycemia: Report of a WHO\/IDF Consultation, World Health Organization."},{"key":"ref_4","unstructured":"World Health Organization (2016). Global Report on Diabetes, World Health Organization."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"38","DOI":"10.14445\/22312803\/IJCTT-V8P108","article-title":"Classification of diabetes mellitus using modified particle swarm optimization and least squares support vector machine","volume":"8","author":"Soliman","year":"2014","journal-title":"Int. J. Comput. Trends Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"S2","DOI":"10.1016\/j.amjmed.2013.06.007","article-title":"The early treatment of type 2 diabetes","volume":"126","author":"Pratley","year":"2013","journal-title":"Am. J. Med."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"(2003). Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Follow-up report on the diagnosis of diabetes mellitus. Diabetes Care, 26, 3160\u20133167.","DOI":"10.2337\/diacare.26.11.3160"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"217","DOI":"10.22543\/2392-7674.1445","article-title":"Diabetes Mellitus: Interdisciplinary Medical, Surgical and Psychological Therapeutic Approach","volume":"10","author":"Socea","year":"2023","journal-title":"J. Mind Med. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"American Diabetes Association (2014). Diagnosis and classification of diabetes mellitus. Diabetes Care, 37, S81\u2013S90.","DOI":"10.2337\/dc14-S081"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"American Diabetes Association (2015). Standards of medical care in diabetes\u20142015: Classification and diagnosis of diabetes mellitus. Diabetes Care, 38, S8\u2013S16.","DOI":"10.2337\/dc15-S005"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"American Diabetes Association (2017). Classification and diagnosis of diabetes. Diabetes Care, 40, S11\u2013S24.","DOI":"10.2337\/dc17-S005"},{"key":"ref_12","unstructured":"The Royal College of Ophthalmologists (2012). Diabetic Retinopathy Guidelines. Section 1: Terminology and Disease Definition, The Royal College of Ophthalmologists."},{"key":"ref_13","first-page":"609","article-title":"Predictors of post-partum persistence of glucose intolerance and its association with cardio-metabolic risk factors in gestational diabetes mellitus","volume":"6","author":"Jahan","year":"2015","journal-title":"J. Diabetes Metab."},{"key":"ref_14","first-page":"1","article-title":"A modern review of diabetes mellitus: An annihilatory metabolic disorder","volume":"3","author":"Deepthi","year":"2017","journal-title":"J. In Silico In Vitro Pharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Canadian Diabetes Association Clinical Practice Guidelines Expert Committee (2013). Definition, classification and diagnosis of diabetes, prediabetes and metabolic syndrome. Can. J. Diabetes, 37, S8\u2013S11.","DOI":"10.1016\/j.jcjd.2013.01.011"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ktenopoulos, N., Anagnostopoulou, L., Apostolos, A., Iliakis, P., Karakasis, P., Milaras, N., Theofilis, P., Fragoulis, C., Drakopoulou, M., and Synetos, A. (2025). Cellular and Molecular Pathways in Diabetes-Associated Heart Failure: Emerging Mechanistic Insights and Therapeutic Opportunities. Curr. Issues Mol. Biol., 47.","DOI":"10.3390\/cimb47110886"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1055\/s-0034-1366278","article-title":"Definition, classification and diagnosis of diabetes mellitus","volume":"122","author":"Kerner","year":"2014","journal-title":"Exp. Clin. Endocrinol. Diabetes"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"National Diabetes Data Group (1979). Classification and diagnosis of diabetes mellitus and other categories of glucose intolerance. Diabetes, 28, 1039\u20131057.","DOI":"10.2337\/diab.28.12.1039"},{"key":"ref_19","unstructured":"World Health Organization (1980). Report of the Expert Committee on Diabetes, World Health Organization. WHO Technical Report Series No. 646."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"373","DOI":"10.15537\/1658-3175.1664","article-title":"History of diabetes mellitus","volume":"23","author":"Ahmed","year":"2002","journal-title":"Saudi Med. J."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Asamoah, A., and Ahima, R.S. (2026). Review Article: Overview of Clinical Genetics of Diabetes Mellitus. Genes, 17.","DOI":"10.3390\/genes17020215"},{"key":"ref_22","first-page":"541","article-title":"Classification, pathophysiology, diagnosis and management of diabetes mellitus","volume":"6","author":"Baynes","year":"2015","journal-title":"J. Diabetes Metab."},{"key":"ref_23","unstructured":"Japan Diabetes Society (2014). Treatment Guide for Diabetes 2014\u20132015, Japan Diabetes Society."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"115734","DOI":"10.1016\/j.biopha.2023.115734","article-title":"Diabetes mellitus: Classification, mediators, and complications; A gate to identify potential targets for the development of new effective treatments","volume":"168","author":"Antar","year":"2023","journal-title":"Biomed. Pharmacother."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Husak, V., Shvadchak, V., Bobrova, O., Faltus, M., Hryhoriv, Y., Karbivska, U., Vatashchuk, M., Hurza, V., and Mel\u2019nyk, V. (2026). Plant-Derived Strategies for Glycemic Management in Diabetes: A Narrative Review. Diabetology, 7.","DOI":"10.3390\/diabetology7020029"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Trasca, D.M., Dop, D., Stoica, G.-A., Adrian, N.S., Carmen, N.E., V\u0103ru\u021b, R.M., and Singer, C.E. (2025). The Antidiabetic Activity of Wild-Growing and Cultivated Medicinal Plants Used in Romania for Diabetes Mellitus Management: A Phytochemical and Pharmacological Review. Pharmaceuticals, 18.","DOI":"10.3390\/ph18071035"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.19082\/1832","article-title":"The role of medicinal plants in the treatment of diabetes: A systematic review","volume":"8","author":"Kooti","year":"2016","journal-title":"Electron. Physician"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Alam, S., Sarker, M.M.R., Sultana, T.N., Chowdhury, M.N.R., Rashid, M.A., Chaity, N.I., Zhao, C., Xiao, J., Hafez, E.E., and Khan, S.A. (2022). Antidiabetic phytochemicals from medicinal plants: Prospective candidates for new drug discovery and development. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.800714"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Salehi, B., Ata, A., Anil Kumar, N.V., Sharopov, F., Ram\u00edrez-Alarc\u00f3n, K., Ruiz-Ortega, A., Ayatollahi, S.A., Fokou, P.V.T., Kobarfard, F., and Zakaria, Z.A. (2019). Antidiabetic potential of medicinal plants and their active components. Biomolecules, 9.","DOI":"10.3390\/biom9100551"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1356893","DOI":"10.1155\/2020\/1356893","article-title":"Antioxidant effects and mechanisms of medicinal plants and their bioactive compounds for the prevention and treatment of type 2 diabetes: An updated review","volume":"2020","author":"Unuofin","year":"2020","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Popescu, M., Radivojevic, K., Trasca, D.-M., Varut, R.M., Enache, I., and Osman, A. (2025). Natural Antidiabetic Agents: Insights into Ericaceae-Derived Phenolics and Their Role in Metabolic and Oxidative Modulation in Diabetes. Pharmaceuticals, 18.","DOI":"10.3390\/ph18050682"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"378657","DOI":"10.1155\/2013\/378657","article-title":"Herbal therapies for type 2 diabetes mellitus: Chemistry, biology, and potential application of selected plants and compounds","volume":"2013","author":"Chang","year":"2013","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Tran, N., Pham, B., and Le, L. (2020). Bioactive compounds in anti-diabetic plants: From herbal medicine to modern drug discovery. Biology, 9.","DOI":"10.3390\/biology9090252"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Sknepnek, A., Mileti\u0107, D., Stupar, A., Salevi\u0107-Jeli\u0107, A., Nedovi\u0107, V., and Cvetanovi\u0107 Kljaki\u0107, A. (2025). Natural solutions for diabetes: The therapeutic potential of plants and mushrooms. Front. Nutr., 12.","DOI":"10.3389\/fnut.2025.1511049"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3742208","DOI":"10.1155\/2022\/3742208","article-title":"Agrimonia pilosa: A phytochemical and pharmacological review","volume":"2022","author":"Jin","year":"2022","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_36","first-page":"96","article-title":"Studies on Agrimoniae herba selective extracts: Polyphenols content, antioxidant and antimicrobial potency, MTS test","volume":"5","author":"Pirvu","year":"2016","journal-title":"Ann. Acad. Rom. Sci. Ser. Biol. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Huzio, N., Grytsyk, A., Raal, A., Grytsyk, L., and Koshovyi, O. (2022). Phytochemical and pharmacological research in Agrimonia eupatoria L. herb extract with anti-inflammatory and hepatoprotective properties. Plants, 11.","DOI":"10.3390\/plants11182371"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Lahlou, R.A., Carvalho, F., Pereira, M.J., Lopes, J., and Silva, L.R. (2024). Overview of ethnobotanical\u2013pharmacological studies carried out on medicinal plants from the Serra da Estrela Natural Park: Focus on their antidiabetic potential. Pharmaceutics, 16.","DOI":"10.20944\/preprints202402.1579.v1"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3607396","DOI":"10.1155\/2024\/3607396","article-title":"Antidiabetic activity, phytochemical analysis, and acute oral toxicity test of combined ethanolic extract of Syzygium polyanthum and Muntingia calabura leaves","volume":"2024","author":"Widodo","year":"2024","journal-title":"Sci. World J."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Ansari, P., Khan, J.T., Chowdhury, S., Reberio, A.D., Kumar, S., Seidel, V., Abdel-Wahab, Y.H.A., and Flatt, P.R. (2024). Plant-based diets and phytochemicals in the management of diabetes mellitus and prevention of its complications: A review. Nutrients, 16.","DOI":"10.20944\/preprints202408.1739.v1"},{"key":"ref_41","first-page":"307","article-title":"Anti-diabetic potential of therapeutic medicinal plants: A review","volume":"9","author":"Selvakumar","year":"2024","journal-title":"Palest. Med. Pharm. J."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Nie, T., and Cooper, G.J.S. (2021). Mechanisms underlying the antidiabetic activities of polyphenolic compounds: A review. Front. Pharmacol., 12.","DOI":"10.3389\/fphar.2021.798329"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"505","DOI":"10.4103\/1735-5362.323917","article-title":"Antidiabetic potential of the isolated fractions from the plants of Rosaceae family in streptozotocin-induced diabetic rats","volume":"16","author":"Bahrami","year":"2021","journal-title":"Res. Pharm. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Kosmala, M., Milala, J., and Karli\u0144ska, E. (2025). Ellagitannins and other polyphenols along with dietary components of the Rosaceae medicinal plants. Molecules, 30.","DOI":"10.3390\/molecules30234574"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Mohanta, Y.K., Mishra, A.K., Nongbet, A., Chakrabartty, I., Mahanta, S., Sarma, B., Panda, J., and Panda, S.K. (2023). Potential use of the Asteraceae family as a cure for diabetes: A review of ethnopharmacology to modern day drug and nutraceuticals developments. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1153600"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ayeni, E.A., and Afolayan, A.J. (2025). Antidiabetic potential of Silybum marianum (L.) Gaertn. and Brachylaena discolor DC. (Asteraceae) in the management of type 2 diabetes mellitus. Plants, 14.","DOI":"10.3390\/plants14213267"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.15414\/jmbfs.2019.8.5.1205-1208","article-title":"Phytochemical profile and antioxidant activity of water pepper (Polygonum hydropiper L.)","volume":"8","author":"Mihaylova","year":"2019","journal-title":"J. Microbiol. Biotechnol. Food Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1080\/13880209.2017.1301493","article-title":"Unlocking the in vitro anti-inflammatory and antidiabetic potential of Polygonum maritimum","volume":"55","author":"Rodrigues","year":"2017","journal-title":"Pharm. Biol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"American Diabetes Association (2016). Standards of medical care in diabetes\u20142016: Classification and diagnosis of diabetes. Diabetes Care, 39, S13\u2013S22.","DOI":"10.2337\/dc16-S005"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4239\/wjd.v7.i1.1","article-title":"Milestones in the history of diabetes mellitus: The main contributors","volume":"7","author":"Karamanou","year":"2016","journal-title":"World J. Diabetes"},{"key":"ref_51","first-page":"WMC004831","article-title":"Diabetes mellitus: A complete ancient and modern historical perspective","volume":"6","author":"Nwaneri","year":"2015","journal-title":"WebmedCentral Diabetes"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1111\/j.1444-0938.1999.tb06760.x","article-title":"Diabetes mellitus: A brief history, epidemiology, definition and classification","volume":"82","author":"Gutteridge","year":"1999","journal-title":"Clin. Exp. Optom."},{"key":"ref_53","first-page":"368","article-title":"The history of diabetes mellitus","volume":"13","author":"Lakhtakia","year":"2013","journal-title":"Sultan Qaboos Univ. Med. J."},{"key":"ref_54","first-page":"217","article-title":"Diabetul zaharat: Epoca modern\u0103 dintr-o perspectiv\u0103 istoric\u0103","volume":"10","year":"2015","journal-title":"Pract. Med."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"82","DOI":"10.2337\/diaspect.27.2.82","article-title":"A brief history of the development of diabetes medications","volume":"27","author":"White","year":"2014","journal-title":"Diabetes Spectr."},{"key":"ref_56","unstructured":"Diabetes Qu\u00e9bec (2016). Treating Diabetes: 1921 to the Present Day, Diabetes Qu\u00e9bec."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.2337\/dc09-9033","article-title":"International Expert Committee report on the role of the A1C assay in the diagnosis of diabetes","volume":"32","author":"Gillett","year":"2009","journal-title":"Diabetes Care"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"McAdams, B.H., and Rizvi, A.A. (2016). An overview of insulin pumps and glucose sensors for the generalist. J. Clin. Med., 5.","DOI":"10.3390\/jcm5010005"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"S85","DOI":"10.1016\/S1262-3636(11)70972-7","article-title":"Advances in pump technology: Insulin patch pumps, combined pumps and glucose sensors, and implanted pumps","volume":"37","author":"Schaepelynck","year":"2011","journal-title":"Diabetes Metab."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Michiu, D., Socaciu, M.-I., Fogarasi, M., Jimborean, A.M., Ranga, F., Mure\u015fan, V., and Semeniuc, C.A. (2022). Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils. Molecules, 27.","DOI":"10.3390\/molecules27041345"},{"key":"ref_61","first-page":"451","article-title":"Current antidiabetic drugs and strategies for the treatment of type 2 diabetes mellitus","volume":"6","author":"Datt","year":"2017","journal-title":"World J. Pharm. Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1016\/j.amjcard.2017.05.009","article-title":"Pharmacologic management of type 2 diabetes mellitus: Available therapies","volume":"120","author":"Thrasher","year":"2017","journal-title":"Am. J. Cardiol."},{"key":"ref_63","first-page":"474","article-title":"Alpha glucosidase inhibitors","volume":"64","author":"Kalra","year":"2014","journal-title":"J. Pak. Med. Assoc."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1517\/14656566.2015.1070827","article-title":"Therapeutic potential of \u03b1-glucosidase inhibitors in type 2 diabetes mellitus: An evidence-based review","volume":"16","author":"Joshi","year":"2015","journal-title":"Expert Opin. Pharmacother."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1016\/j.mpmed.2014.09.007","article-title":"Epidemiology of diabetes","volume":"42","author":"Forouhi","year":"2014","journal-title":"Medicine"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"232","DOI":"10.2337\/diacare.15.2.232","article-title":"Kelly West Lecture 1991. Challenges in diabetes epidemiology\u2014From West to the rest","volume":"15","author":"Zimmet","year":"1992","journal-title":"Diabetes Care"},{"key":"ref_67","unstructured":"International Diabetes Federation (2015). IDF Diabetes Atlas, International Diabetes Federation. [7th ed.]. Available online: http:\/\/diabetesatlas.org."},{"key":"ref_68","unstructured":"International Diabetes Federation (2013). IDF Diabetes Atlas, International Diabetes Federation. [6th ed.]. Available online: https:\/\/diabetesatlas.org\/resources\/previous-editions\/."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Porwal, M., Khan, N.A., and Maheshwari, K.K. (2017). Evaluation of acute and subacute oral toxicity induced by ethanolic extract of Marsdenia tenacissima leaves in experimental rats. Sci. Pharm., 85.","DOI":"10.3390\/scipharm85030029"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Benrahou, K., Mrabti, H.N., Assaggaf, H.M., Mortada, S., Salhi, N., Rouas, L., El Bacha, R., Dami, A., Masrar, A., and Alshahrani, M.M. (2022). Acute and subacute toxicity studies of Erodium guttatum extracts by oral administration in rodents. 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