{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:59:26Z","timestamp":1787029166919,"version":"build-2736575974"},"reference-count":42,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study explores an innovative approach based on deep eutectic solvent (DES)-synergistic ultrasonic-assisted extraction (UAE) of anthocyanins from blueberry pomace (BP). Choline chloride\u2013lactic acid (ChCl-LA, 1:2) was identified as the most efficient DES and provided the highest anthocyanin recovery. The optimal conditions for ChCl-LA-synergistic UAE (ChCl-LA-UAE) were obtained as follows: ultrasonic power of 270 W, extraction time of 30 min, liquid-to-solid ratio of 35:1, and water content in ChCl-LA of 40%, achieving a total anthocyanin content (TAC) of 3.5168 mg\/g, with R2 = 0.9860. This value was significantly higher than those obtained with 70% ethanol (3.1962 mg\/g) and pure water (1.9137 mg\/g). SEM images revealed that ChCl-LA-UAE disrupted the surface structure of the samples, thereby promoting anthocyanin release. COSMO-RS simulations confirmed that ChCl-LA significantly enhanced the interaction between the solvent and the representative anthocyanin (cyanidin-3-O-glucoside, C3G), resulting in higher extraction efficiency. In vitro antioxidant assays further demonstrated that the synergistic system exhibited stronger antioxidant activity. Overall, ultrasonic-synergistic DES extraction could be an eco-friendly method for recovering high-value compounds from blueberry and its byproducts.<\/jats:p>","DOI":"10.3390\/molecules31132356","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T14:33:06Z","timestamp":1783089186000},"page":"2356","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Deep Eutectic Solvent-Assisted Ultrasonic Extraction of Anthocyanins from Blueberry Pomace: Optimization, Mechanistic Insights and In Vitro Antioxidant Activity"],"prefix":"10.3390","volume":"31","author":[{"given":"Lina","family":"Chen","sequence":"first","affiliation":[{"name":"College of Chemistry, Changchun Normal University, Changchun 130032, China"},{"name":"Institute of Innovation Science and Technology, Changchun Normal University, Changchun 130032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Mi","sequence":"additional","affiliation":[{"name":"Institute of Innovation Science and Technology, Changchun Normal University, Changchun 130032, China"},{"name":"College of Life Science, Changchun Normal University, Changchun 130032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Innovation Science and Technology, Changchun Normal University, Changchun 130032, China"},{"name":"College of Life Science, Changchun Normal University, Changchun 130032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunmei","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Innovation Science and Technology, Changchun Normal University, Changchun 130032, China"},{"name":"College of Life Science, Changchun Normal University, Changchun 130032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3096-9457","authenticated-orcid":false,"given":"Chunting","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Innovation Science and Technology, Changchun Normal University, Changchun 130032, China"},{"name":"College of Life Science, Changchun Normal University, Changchun 130032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Innovation Science and Technology, Changchun Normal University, Changchun 130032, China"},{"name":"College of Life Science, Changchun Normal University, Changchun 130032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongfang","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute of Innovation Science and Technology, Changchun Normal University, Changchun 130032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100476","DOI":"10.1016\/j.fochx.2022.100476","article-title":"Recovering high value-added anthocyanins from blueberry pomace with ultrasound-assisted extraction","volume":"16","author":"Zhang","year":"2022","journal-title":"Food Chem. X"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/s12560-016-9247-3","article-title":"Reduction of enteric viruses by blueberry juice and blueberry proanthocyanidins","volume":"8","author":"Joshi","year":"2016","journal-title":"Food Environ. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ivarsson, J., Pecorelli, A., Lila, M.A., and Valacchi, G. (2023). Blueberry supplementation and skin health. Antioxidants, 12.","DOI":"10.3390\/antiox12061261"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"102459","DOI":"10.1016\/j.fochx.2025.102459","article-title":"Active ingredients of blueberry pomace: A comprehensive review of isolation, identification, health benefits and food applications","volume":"27","author":"Huang","year":"2025","journal-title":"Food Chem. X"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"125181","DOI":"10.1016\/j.biortech.2021.125181","article-title":"Sustainable blueberry waste recycling towards biorefinery strategy and circular bioeconomy: A review","volume":"332","author":"Liu","year":"2021","journal-title":"Bioresour. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1007\/s00217-026-05132-6","article-title":"Fruit peel\u2013derived bioactives for type 2 diabetes management","volume":"252","author":"Kaur","year":"2026","journal-title":"Eur. Food Res. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e15509","DOI":"10.1111\/jfpp.15509","article-title":"Antioxidant activities and inhibitory effects of blueberry pomace and wine pomace crude extracts on oxidation of oil in water emulsion and fish mince","volume":"45","author":"Dai","year":"2021","journal-title":"J. Food Process. Preserv."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Mattioli, R., Francioso, A., Mosca, L., and Silva, P. (2020). Anthocyanins: A comprehensive review of their chemical properties and health effects on cardiovascular and neurodegenerative diseases. Molecules, 25.","DOI":"10.3390\/molecules25173809"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.1002\/ptr.6485","article-title":"Anthocyanidin attenuates myocardial ischemia induced injury via inhibition of ROS-JNK-Bcl-2 pathway: New mechanism of anthocyanidin action","volume":"33","author":"Syeda","year":"2019","journal-title":"Phytother. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1080\/15538362.2017.1285264","article-title":"Characterization of pilot scale processed Canadian organic cranberry (Vaccinium macrocarpon) and blueberry (Vaccinium angustifolium) juice pressing residues and phenolic-enriched extractives","volume":"17","author":"Ross","year":"2017","journal-title":"Int. J. Fruit Sci."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Bamba, B.S.B., Shi, J., Tranchant, C.C., Xue, S.J., Forney, C.F., and Lim, L.-T. (2018). Influence of extraction conditions on ultrasound-assisted recovery of bioactive phenolics from blueberry pomace and their antioxidant activity. Molecules, 23.","DOI":"10.3390\/molecules23071685"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"109260","DOI":"10.1016\/j.lwt.2020.109260","article-title":"Fermented blueberry pomace with antioxidant properties improves fecal microbiota community structure and short chain fatty acids production in an in vitro mode","volume":"125","author":"Cheng","year":"2020","journal-title":"Lwt"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zhu, C.-W., L\u00fc, H., Du, L.-L., Li, J., Chen, H., Zhao, H.-F., Wu, W.-L., Chen, J., and Li, W.-L. (2023). Five blueberry anthocyanins and their antioxidant, hypoglycemic, and hypolipidemic effects in vitro. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1172982"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"110064","DOI":"10.1016\/j.lwt.2020.110064","article-title":"Citric acid water-based solution for blueberry bagasse anthocyanins recovery: Optimization and comparisons with microwave-assisted extraction (MAE)","volume":"133","author":"Ferreira","year":"2020","journal-title":"Lwt"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1021\/jf3046158","article-title":"Composition of polyphenols and antioxidant activity of rabbiteye blueberry (Vaccinium ashei) in Nanjing","volume":"61","author":"Li","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"106572","DOI":"10.1016\/j.foodhyd.2020.106572","article-title":"Structure and composition of blueberry fiber pectin and xyloglucan that bind anthocyanins during fruit puree processing","volume":"116","author":"Hotchkiss","year":"2021","journal-title":"Food Hydrocoll."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Chima, B., Mathews, P., Morgan, S., Johnson, S.A., and Van Buiten, C.B. (2022). Physicochemical characterization of interactions between blueberry polyphenols and food proteins from dairy and plant sources. Foods, 11.","DOI":"10.3390\/foods11182846"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"136224","DOI":"10.1016\/j.foodchem.2023.136224","article-title":"Deep eutectic solvent combined with ultrasound technology: A promising integrated extraction strategy for anthocyanins and polyphenols from blueberry pomace","volume":"422","author":"Zhang","year":"2023","journal-title":"Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"124790","DOI":"10.1016\/j.molliq.2024.124790","article-title":"Separation of polyphenolic compounds from Citrus aurantium L. peel by deep eutectic solvents and their recovery using a new DES-based aqueous two-phase system","volume":"402","author":"Edrisi","year":"2024","journal-title":"J. Mol. Liq."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.1007\/s00217-025-04797-9","article-title":"A systematic review of recent advances in anthocyanin-biomacromolecule interactions and their applications in the food industry","volume":"251","author":"Tao","year":"2025","journal-title":"Eur. Food Res. Technol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"182","DOI":"10.3109\/13880200903062671","article-title":"Free radical scavenging and anti-acne activities of mangosteen fruit rind extracts prepared by different extraction methods","volume":"48","author":"Pothitirat","year":"2010","journal-title":"Pharm. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"9985604","DOI":"10.1155\/2021\/9985604","article-title":"Novel \u03b1-Mangostin Derivatives from Mangosteen (Garcinia mangostana L.) Peel Extract with Antioxidant and Anticancer Potential","volume":"2021","author":"Tran","year":"2021","journal-title":"J. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1039\/b210714g","article-title":"Novel solvent properties of choline chloride\/urea mixtures","volume":"39","author":"Abbott","year":"2003","journal-title":"Chem. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"106102","DOI":"10.1016\/j.ultsonch.2022.106102","article-title":"Composition and antioxidant activity of anthocyanins from Aronia melanocarpa extracted using an ultrasonic-microwave-assisted natural deep eutectic solvent extraction method","volume":"89","author":"Lin","year":"2022","journal-title":"Ultrason. Sonochem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e70075","DOI":"10.1002\/sscp.70075","article-title":"Natural deep eutectic solvents in extraction science: Progress, challenges, and future prospects","volume":"8","author":"Suthar","year":"2025","journal-title":"Sep. Sci. Plus"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1039\/D4SU00560K","article-title":"A review of designable deep eutectic solvents for green fabrication of advanced functional materials","volume":"3","author":"Wang","year":"2025","journal-title":"RSC Sustain."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e16732","DOI":"10.1111\/jfpp.16732","article-title":"Effects of process parameters on the extraction of total anthocyanins from purple sweet potatoes by ultrasound with wide frequency and its kinetics study","volume":"46","author":"Liao","year":"2022","journal-title":"J. Food Process. Preserv."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Lon\u010dari\u0107, A., Celeiro, M., Jozinovi\u0107, A., Jelini\u0107, J., Kova\u010d, T., Joki\u0107, S., Babi\u0107, J., Moslavac, T., Zavadlav, S., and Lores, M. (2020). Green extraction methods for extraction of polyphenolic compounds from blueberry pomace. Foods, 9.","DOI":"10.3390\/foods9111521"},{"key":"ref_29","first-page":"1","article-title":"Bibliometric analysis and literature review of ultrasound-assisted extraction","volume":"67","author":"Kusuma","year":"2024","journal-title":"Egypt. J. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1093\/jaoacint\/qsaa150","article-title":"Multi-frequency ultrasonic extraction of anthocyanins from blueberry pomace and evaluation of its antioxidant activity","volume":"104","author":"Hu","year":"2021","journal-title":"J. AOAC Int."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"129577","DOI":"10.1016\/j.foodchem.2021.129577","article-title":"Sonication-synergistic natural deep eutectic solvent as a green and efficient approach for extraction of phenolic compounds from peels of Carya cathayensis Sarg","volume":"355","author":"Fu","year":"2021","journal-title":"Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"140046","DOI":"10.1016\/j.foodchem.2024.140046","article-title":"A high-permeability method for extracting purple yam saponins based on ultrasonic-assisted natural deep eutectic solvent","volume":"457","author":"Hou","year":"2024","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"133226","DOI":"10.1016\/j.foodchem.2022.133226","article-title":"Acidified glycerol as a one-step efficient green extraction and preservation strategy for anthocyanin from blueberry pomace: New insights into extraction and stability protection mechanism with molecular dynamic simulation","volume":"390","author":"Fu","year":"2022","journal-title":"Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"120237","DOI":"10.1016\/j.indcrop.2024.120237","article-title":"Natural deep eutectic solvents extraction of anthocyanins\u2013effective method for valorisation of black raspberry (Rubus occidentalis L.) pomace","volume":"223","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"103470","DOI":"10.1016\/j.jfca.2020.103470","article-title":"Natural deep eutectic solvents as a biocompatible tool for the extraction of blueberry anthocyanins","volume":"89","author":"Pauletto","year":"2020","journal-title":"J. Food Compos. Anal."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.3390\/molecules21091264","article-title":"Cyanidin-3-O-glucoside: Physical-chemistry, foodomics and health effects","volume":"21","year":"2016","journal-title":"Molecules"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Gon\u00e7alves, A.C., Nunes, A.R., Falc\u00e3o, A., Alves, G., and Silva, L.R. (2021). Dietary effects of anthocyanins in human health: A comprehensive review. Pharmaceuticals, 14.","DOI":"10.3390\/ph14070690"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1016\/j.foodres.2010.01.022","article-title":"The properties and stability of anthocyanins in mulberry fruits","volume":"43","author":"Aramwit","year":"2010","journal-title":"Food Res. Int."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"142648","DOI":"10.1016\/j.foodchem.2024.142648","article-title":"Advancing anthocyanin extraction: Optimising solvent, preservation, and microwave techniques for enhanced recovery from merlot grape marc","volume":"472","author":"Yang","year":"2025","journal-title":"Food Chem."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Muti, L., Nascimento, L.B.d.S., Goracci, G., Detti, C., Brunetti, C., Bilia, A.R., Ferrini, F., and Gori, A. (2025). From Waste to Value: Optimization of Ultrasound-Assisted Extraction of Anthocyanins and Flavonols from Pistacia lentiscus L. Oilcakes. Molecules, 30.","DOI":"10.3390\/molecules30020237"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.foodchem.2016.01.048","article-title":"In vitro antioxidant and anti-inflammatory properties of Limonium algarvense flowers\u2019 infusions and decoctions: A comparison with green tea (Camellia sinensis)","volume":"200","author":"Rodrigues","year":"2016","journal-title":"Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3694","DOI":"10.1111\/1750-3841.16706","article-title":"Bergamot juice powder with high bioactive properties: Spray-drying for the preservation of antioxidant activity and ultrasound-assisted extraction for enhanced phenolic compound extraction","volume":"88","author":"Demircan","year":"2023","journal-title":"J. Food Sci."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/13\/2356\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T04:36:00Z","timestamp":1783485360000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/13\/2356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,3]]},"references-count":42,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["molecules31132356"],"URL":"https:\/\/doi.org\/10.3390\/molecules31132356","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,3]]}}}