{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:37:17Z","timestamp":1790372237770,"version":"4.1.0"},"reference-count":27,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:00:00Z","timestamp":1783728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["314858\/2020-2"],"award-info":[{"award-number":["314858\/2020-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"award":["314858\/2020-2"],"award-info":[{"award-number":["314858\/2020-2"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005672","name":"Funda\u00e7\u00e3o de Apoio ao Desenvolvimento do Ensino, Ci\u00eancia e Tecnologia do Estado de Mato Grosso do Sul","doi-asserted-by":"crossref","award":["71\/020.508\/2022"],"award-info":[{"award-number":["71\/020.508\/2022"]}],"id":[{"id":"10.13039\/501100005672","id-type":"DOI","asserted-by":"crossref"}]},{"award":["71\/020.508\/2022"],"award-info":[{"award-number":["71\/020.508\/2022"]}],"id":[{"id":"https:\/\/ror.org\/05jtrt935","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004809","name":"Financiadora de Estudos e Projetos","doi-asserted-by":"crossref","award":["01.19.0124.00"],"award-info":[{"award-number":["01.19.0124.00"]}],"id":[{"id":"10.13039\/501100004809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["01.19.0124.00"],"award-info":[{"award-number":["01.19.0124.00"]}],"id":[{"id":"https:\/\/ror.org\/030w99567","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"https:\/\/ror.org\/00x0ma614","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Cancer therapy remains limited by drug resistance and poor selectivity, while inflammation-driven tumor progression further complicates treatment outcomes. Antimicrobial peptides (AMPs) have emerged as promising therapeutic alternatives due to their multifunctional properties. In this study, we investigated the anticancer and immunomodulatory activities of KI17, a rationally designed peptide derived from GL18, a peptide fragment identified from the talisin protein of Talisia esculenta. KI17 exhibited dose-dependent antiproliferative effects against murine and human melanoma (B16F10-Nex2, SK-MEL-2, A375) and cervical cancer (HeLa) cell lines, while displaying reduced cytotoxicity toward non-tumoral BV-2 microglial cells, resulting in a favorable selectivity index. Mechanistic analyses revealed that KI17 induces morphological alterations, mitochondrial dysfunction, caspase activation, and late-stage apoptosis, together with G0\/G1 cell cycle arrest accompanied by accumulation of the Sub-G0 population, indicating coordinated regulation of cell death and cell cycle progression. KI17 effectively suppressed lipopolysaccharide (LPS)-induced microglial activation, markedly reducing pro-inflammatory cytokine and nitric oxide production without compromising cell viability. These biological activities are consistent with the peptide\u2019s optimized physicochemical features, including increased cationicity, amphipathicity, and \u03b1-helical folding. Overall, our findings demonstrate that KI17 combines selective anticancer activity with potent immunomodulatory effects, highlighting its potential as a bioinspired peptide for further preclinical development in cancer therapy.<\/jats:p>","DOI":"10.3390\/molecules31142434","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T08:05:31Z","timestamp":1783929931000},"page":"2434","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["KI17: A Bioinspired Peptide Derived from Talisia esculenta with In Vitro Anticancer and Immunomodulatory Activities"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9620-861X","authenticated-orcid":false,"given":"Ana Paula Ramos","family":"Pereira","sequence":"first","affiliation":[{"name":"Laboratory of Protein Purification and Biological Functions, FACFAN, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8233-0713","authenticated-orcid":false,"given":"Ana Cristina","family":"Jacobowski","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Biological Functions, FACFAN, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila de Oliveira","family":"Gutierrez","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Biological Functions, FACFAN, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oct\u00e1vio Luiz","family":"Franco","sequence":"additional","affiliation":[{"name":"S-Inova Biotech, Graduate Program in Biotechnology, Dom Bosco Catholic University, Campo Grande 79117-900, MS, Brazil"},{"name":"Center for Proteomic and Biochemical Analysis, Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Bras\u00edlia, Bras\u00edlia 71966-700, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6676-5362","authenticated-orcid":false,"given":"Marlon Henrique","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Center for Proteomic and Biochemical Analysis, Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Bras\u00edlia, Bras\u00edlia 71966-700, DF, Brazil"},{"name":"Graduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande 79117-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1937-0162","authenticated-orcid":false,"given":"Tha\u00eds de Andrade Farias","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"CeTroGen Laboratory, Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3514-3346","authenticated-orcid":false,"given":"Rodrigo Juliano","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CeTroGen Laboratory, Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1115-4083","authenticated-orcid":false,"given":"Priscila Aiko","family":"Hiane","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Food and Nutrition, Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita de C\u00e1ssia Avellaneda","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Food and Nutrition, Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula de Ara\u00fajo","family":"Boleti","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Biological Functions, FACFAN, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6969-6307","authenticated-orcid":false,"given":"Maria L\u00edgia Rodrigues","family":"Macedo","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Biological Functions, FACFAN, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"},{"name":"Faculty of Pharmaceutical Sciences, Food and Nutrition, Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, 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